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The map label for this gene is spoIVB [H]

Identifier: 152976577

GI number: 152976577

Start: 2939223

End: 2940521

Strand: Reverse

Name: spoIVB [H]

Synonym: Bcer98_2866

Alternate gene names: 152976577

Gene position: 2940521-2939223 (Counterclockwise)

Preceding gene: 152976578

Following gene: 152976576

Centisome position: 71.95

GC content: 36.87

Gene sequence:

>1299_bases
GTGAATAAATTGAGATTAGAACACTTCCGGAAAATAATAGGGATTTGTCTCCTTGTTTCGTTAGTTTTTATTGTCTGTTT
TAAACCATTACGTACATTTATTTCATTTCCAAAACAACTTGTTATTTTTGAAGGGGAACAATCAGAAATAGAATCACTGC
CTGTTTTTCAAGCTTCATCTACCAATCATAACGTGTTTACCGTAAGTTCTAAACAAAAAGCGCCGGGGATTATGGTGAAT
TCACATCAAAATGGTGAGGCGGATATGGTGTTTCAATTGGCTGGTTTCCCTGTGAAGAAAGTAAACGTAAAAGTGTTAAA
AGATTTTAAAGTTATTCCAGGTGGACAATCTATCGGTGTGAAGCTGAATACGAAAGGTGTCCTTGTTGTTGGACATCATT
TAATTCAAACGGAAAAAGGGAAAGTATCGCCAGGTGAACTAGCAGGTGTACAAGTTGGTGATATGATTACTGCAATTAAT
GGAAAAACAATCGAAAGAATGAGCGATGTAGCACCATTTATTCATAGTAGTGGTGAAACAGGTGAACCGCTTCATCTTGT
GTTATTGAGGGATGGGAAGCATATTCGCACAAAGCTAACGCCACAAAAAGATAGTGGGGAATCATCTTATCGCATTGGTC
TATATATTCGTGATTCGGCAGCTGGTATTGGAACGATGACATTTATCGACCCAAATTCCATGAAATATGGAGCGCTTGGT
CATGTTATTTCTGACAATGATACAAAAAAACCGATTCAAGTGGAAGATGGGCAAATTATGCGTTCGACAGTCACTTCGAT
TGAAAGAGGAAGCCATGGAAATCCCGGAGAAAAGTTAGCGAAATTCTCACCAGATCATGAAGTAATTGGGAATATTACAA
CAAATAGTCCATTTGGAATTTTTGGAAAGCTAAATACAAATGTGAAAAACGGGATAATGGATAAGGCGATGCCAATCGCA
TTATCTCATCAAGTAAAAGAAGGACCAGCAAAAATATTAACAGTAATTGATAACGATAAAGTAGAATCATTTGATGTTGA
CATAGTAAGTGCAGTGCCGCAAAAATTTCCAGCTACAAAAGGAATGGTTATAAAGGTTACTGATAAGCGTTTATTAGAAA
AAACAGGTGGTATTGTACAAGGAATGAGCGGAAGCCCAATTGTTCAAAATGGAAAAGTGATTGGTGCAGTTACACATGTA
TTTGTAAATGACCCAACTTCCGGATATGGTGTGCACATTGAATGGATGTTACATGAAGCGGGAATCAATATTTATGAACA
AAATAAAAAAGCAAGCTGA

Upstream 100 bases:

>100_bases
AATAATGATTTCTTAATTCTTCCGGTTATAAACAGGGCGTTGCAAGGAGAGATTAAAAGATGAGGCCGAAGAGGCTGAAT
GTGGGGTAGGAGCGAGGAGA

Downstream 100 bases:

>100_bases
TGTGTATCAGCTTGCTTTTTTATTGAGAAATTCGTTGAAATTTTGGAAAAGCTCCGCAAGAGCTGTTTTTTGTTGTAAAA
TAAAAGAAAAGAATGGATAT

Product: peptidase S55 sporulation stage IV protein B

Products: NA

Alternate protein names: Sporulation factor IV B protease; Stage IV sporulation protein B [H]

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MNKLRLEHFRKIIGICLLVSLVFIVCFKPLRTFISFPKQLVIFEGEQSEIESLPVFQASSTNHNVFTVSSKQKAPGIMVN
SHQNGEADMVFQLAGFPVKKVNVKVLKDFKVIPGGQSIGVKLNTKGVLVVGHHLIQTEKGKVSPGELAGVQVGDMITAIN
GKTIERMSDVAPFIHSSGETGEPLHLVLLRDGKHIRTKLTPQKDSGESSYRIGLYIRDSAAGIGTMTFIDPNSMKYGALG
HVISDNDTKKPIQVEDGQIMRSTVTSIERGSHGNPGEKLAKFSPDHEVIGNITTNSPFGIFGKLNTNVKNGIMDKAMPIA
LSHQVKEGPAKILTVIDNDKVESFDVDIVSAVPQKFPATKGMVIKVTDKRLLEKTGGIVQGMSGSPIVQNGKVIGAVTHV
FVNDPTSGYGVHIEWMLHEAGINIYEQNKKAS

Sequences:

>Translated_432_residues
MNKLRLEHFRKIIGICLLVSLVFIVCFKPLRTFISFPKQLVIFEGEQSEIESLPVFQASSTNHNVFTVSSKQKAPGIMVN
SHQNGEADMVFQLAGFPVKKVNVKVLKDFKVIPGGQSIGVKLNTKGVLVVGHHLIQTEKGKVSPGELAGVQVGDMITAIN
GKTIERMSDVAPFIHSSGETGEPLHLVLLRDGKHIRTKLTPQKDSGESSYRIGLYIRDSAAGIGTMTFIDPNSMKYGALG
HVISDNDTKKPIQVEDGQIMRSTVTSIERGSHGNPGEKLAKFSPDHEVIGNITTNSPFGIFGKLNTNVKNGIMDKAMPIA
LSHQVKEGPAKILTVIDNDKVESFDVDIVSAVPQKFPATKGMVIKVTDKRLLEKTGGIVQGMSGSPIVQNGKVIGAVTHV
FVNDPTSGYGVHIEWMLHEAGINIYEQNKKAS
>Mature_432_residues
MNKLRLEHFRKIIGICLLVSLVFIVCFKPLRTFISFPKQLVIFEGEQSEIESLPVFQASSTNHNVFTVSSKQKAPGIMVN
SHQNGEADMVFQLAGFPVKKVNVKVLKDFKVIPGGQSIGVKLNTKGVLVVGHHLIQTEKGKVSPGELAGVQVGDMITAIN
GKTIERMSDVAPFIHSSGETGEPLHLVLLRDGKHIRTKLTPQKDSGESSYRIGLYIRDSAAGIGTMTFIDPNSMKYGALG
HVISDNDTKKPIQVEDGQIMRSTVTSIERGSHGNPGEKLAKFSPDHEVIGNITTNSPFGIFGKLNTNVKNGIMDKAMPIA
LSHQVKEGPAKILTVIDNDKVESFDVDIVSAVPQKFPATKGMVIKVTDKRLLEKTGGIVQGMSGSPIVQNGKVIGAVTHV
FVNDPTSGYGVHIEWMLHEAGINIYEQNKKAS

Specific function: Plays a central role in the sigma-K checkpoint which coordinates gene expression during the later stages of spore formation. The protease is activated by trans cleavage of the zymogen precursor producing SpoIVB-45 kDa. This undergoes further trimming by c

COG id: COG0750

COG function: function code M; Predicted membrane-associated Zn-dependent proteases 1

Gene ontology:

Cell location: Forespore intermembrane space [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 peptidase S55 domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001478
- InterPro:   IPR014219
- InterPro:   IPR008763 [H]

Pfam domain/function: PF00595 PDZ; PF05580 Peptidase_S55 [H]

EC number: =3.4.21.116 [H]

Molecular weight: Translated: 46877; Mature: 46877

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKLRLEHFRKIIGICLLVSLVFIVCFKPLRTFISFPKQLVIFEGEQSEIESLPVFQASS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHCCCEEEECC
TNHNVFTVSSKQKAPGIMVNSHQNGEADMVFQLAGFPVKKVNVKVLKDFKVIPGGQSIGV
CCCEEEEEECCCCCCCEEEECCCCCCCEEEEEECCCCEEEEEEEEEEEEEECCCCCEEEE
KLNTKGVLVVGHHLIQTEKGKVSPGELAGVQVGDMITAINGKTIERMSDVAPFIHSSGET
EECCCEEEEEEEEEEECCCCCCCCCCCCCEECCCEEEECCCCHHHHHHHHHHHHHCCCCC
GEPLHLVLLRDGKHIRTKLTPQKDSGESSYRIGLYIRDSAAGIGTMTFIDPNSMKYGALG
CCCEEEEEEECCCEEEEECCCCCCCCCCCEEEEEEEECCCCCCEEEEEECCCCCCCCCCC
HVISDNDTKKPIQVEDGQIMRSTVTSIERGSHGNPGEKLAKFSPDHEVIGNITTNSPFGI
EEECCCCCCCCEEECCCCHHHHHHHHHHCCCCCCCHHHHHHCCCCHHEEEEEECCCCCEE
FGKLNTNVKNGIMDKAMPIALSHQVKEGPAKILTVIDNDKVESFDVDIVSAVPQKFPATK
EEEECCCHHHCCHHCCCCEEEHHHCCCCCEEEEEEEECCCCCEECCHHHHHCHHHCCCCC
GMVIKVTDKRLLEKTGGIVQGMSGSPIVQNGKVIGAVTHVFVNDPTSGYGVHIEWMLHEA
CEEEEECCHHHHHHCCCEEECCCCCCEEECCEEEEEEEEEEECCCCCCCEEEEEEEEEEC
GINIYEQNKKAS
CCCEEECCCCCC
>Mature Secondary Structure
MNKLRLEHFRKIIGICLLVSLVFIVCFKPLRTFISFPKQLVIFEGEQSEIESLPVFQASS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHCCCEEEECC
TNHNVFTVSSKQKAPGIMVNSHQNGEADMVFQLAGFPVKKVNVKVLKDFKVIPGGQSIGV
CCCEEEEEECCCCCCCEEEECCCCCCCEEEEEECCCCEEEEEEEEEEEEEECCCCCEEEE
KLNTKGVLVVGHHLIQTEKGKVSPGELAGVQVGDMITAINGKTIERMSDVAPFIHSSGET
EECCCEEEEEEEEEEECCCCCCCCCCCCCEECCCEEEECCCCHHHHHHHHHHHHHCCCCC
GEPLHLVLLRDGKHIRTKLTPQKDSGESSYRIGLYIRDSAAGIGTMTFIDPNSMKYGALG
CCCEEEEEEECCCEEEEECCCCCCCCCCCEEEEEEEECCCCCCEEEEEECCCCCCCCCCC
HVISDNDTKKPIQVEDGQIMRSTVTSIERGSHGNPGEKLAKFSPDHEVIGNITTNSPFGI
EEECCCCCCCCEEECCCCHHHHHHHHHHCCCCCCCHHHHHHCCCCHHEEEEEECCCCCEE
FGKLNTNVKNGIMDKAMPIALSHQVKEGPAKILTVIDNDKVESFDVDIVSAVPQKFPATK
EEEECCCHHHCCHHCCCCEEEHHHCCCCCEEEEEEEECCCCCEECCHHHHHCHHHCCCCC
GMVIKVTDKRLLEKTGGIVQGMSGSPIVQNGKVIGAVTHVFVNDPTSGYGVHIEWMLHEA
CEEEEECCHHHHHHCCCEEECCCCCCEEECCEEEEEEEEEEECCCCCCCEEEEEEEEEEC
GINIYEQNKKAS
CCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2106508; 8969508; 9384377; 11741860 [H]