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The map label for this gene is spo0A [H]

Identifier: 152976576

GI number: 152976576

Start: 2938143

End: 2938937

Strand: Reverse

Name: spo0A [H]

Synonym: Bcer98_2865

Alternate gene names: 152976576

Gene position: 2938937-2938143 (Counterclockwise)

Preceding gene: 152976577

Following gene: 152976574

Centisome position: 71.91

GC content: 36.23

Gene sequence:

>795_bases
GTGGAGAAAATCAAAGTATGTCTTGTGGATGATAATAAAGAATTGGTATCAATGCTAGAAAGTTATGTTGCTGCCCAAGA
TGATATGGAAGTAATCGGAACGGCTTATAATGGTCAAGAATGTTTAAATTTATTAAAAGAAAAACAACCAGATGTACTCG
TTTTAGACATTATTATGCCACATTTAGATGGCCTAGCAGTACTGGAGAAAATGCGACATATTGAACGACTAAAACAACCG
AATGTTATTATGTTAACAGCATTTGGGCAAGAAGATGTGACAAAGAAAGCAGTTGATCTAGGAGCTTCTTATTTCATATT
AAAACCATTTGATATGGAGAATTTAACGAGTCATATTCGTCAAGTAAGTGGTAAAACAAATGCTGCGATAAAACGCCCAC
TACCATCTTTCCGATCGGTAACAACAGCAGATGGAAAACCGAAAAATTTAGATGCGAGTATTACGAGCATTATTCACGAA
ATTGGTGTCCCAGCTCATATTAAAGGATATATGTATTTACGCGAAGCAATTTCTATGGTGTACAACGACATTGAGTTGTT
AGGATCTATTACGAAAGTATTATATCCAGATATCGCTAAGAAATATAATACAACTGCAAGTCGTGTGGAACGTGCAATTC
GACATGCGATTGAAGTAGCATGGAGCCGTGGGAATATTGATTCTATCTCATCTTTATTCGGTTATACCGTATCCATGTCA
AAAGCAAAACCGACGAATTCCGAATTTATTGCAATGGTTGCGGATAAATTAAGGCTTGAACATAAGGCGAGTTGA

Upstream 100 bases:

>100_bases
GAAACTTTAAAAGGGAAATTTCACACAATTGTCGAACAATTCATGTAGCCTAGAAGCTTACACATGTCGGAAAATTTGAT
TCGGTGAGGGAGGAAAAGCT

Downstream 100 bases:

>100_bases
AATTATTAGTAACGACTCTAATATTATTAGAGAGCGTTGAGCGATACATGTTTTTTCGGACCGATCAATAACTCGGTCGT
TTGCTGATGAACACTGTTCT

Product: response regulator receiver protein

Products: protein L-glutamate+ methanol; methanol; Tapglu; methanol; Targlu; Trgglu; Tsrglu; NH3; protein-L-glutamate [C]

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MEKIKVCLVDDNKELVSMLESYVAAQDDMEVIGTAYNGQECLNLLKEKQPDVLVLDIIMPHLDGLAVLEKMRHIERLKQP
NVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLTSHIRQVSGKTNAAIKRPLPSFRSVTTADGKPKNLDASITSIIHE
IGVPAHIKGYMYLREAISMVYNDIELLGSITKVLYPDIAKKYNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTVSMS
KAKPTNSEFIAMVADKLRLEHKAS

Sequences:

>Translated_264_residues
MEKIKVCLVDDNKELVSMLESYVAAQDDMEVIGTAYNGQECLNLLKEKQPDVLVLDIIMPHLDGLAVLEKMRHIERLKQP
NVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLTSHIRQVSGKTNAAIKRPLPSFRSVTTADGKPKNLDASITSIIHE
IGVPAHIKGYMYLREAISMVYNDIELLGSITKVLYPDIAKKYNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTVSMS
KAKPTNSEFIAMVADKLRLEHKAS
>Mature_264_residues
MEKIKVCLVDDNKELVSMLESYVAAQDDMEVIGTAYNGQECLNLLKEKQPDVLVLDIIMPHLDGLAVLEKMRHIERLKQP
NVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLTSHIRQVSGKTNAAIKRPLPSFRSVTTADGKPKNLDASITSIIHE
IGVPAHIKGYMYLREAISMVYNDIELLGSITKVLYPDIAKKYNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTVSMS
KAKPTNSEFIAMVADKLRLEHKAS

Specific function: May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with Spo0H (a sigma factor) to control the ex

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788192, Length=109, Percent_Identity=38.5321100917431, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788550, Length=111, Percent_Identity=35.1351351351351, Blast_Score=67, Evalue=9e-13,
Organism=Escherichia coli, GI1790299, Length=140, Percent_Identity=32.8571428571429, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI87082052, Length=130, Percent_Identity=31.5384615384615, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR014879
- InterPro:   IPR012052
- InterPro:   IPR011991
- ProDom:   PD010908 [H]

Pfam domain/function: PF00072 Response_reg; PF08769 Spo0A_C [H]

EC number: 3.1.1.61 [C]

Molecular weight: Translated: 29454; Mature: 29454

Theoretical pI: Translated: 7.69; Mature: 7.69

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKIKVCLVDDNKELVSMLESYVAAQDDMEVIGTAYNGQECLNLLKEKQPDVLVLDIIMP
CCCEEEEEECCCHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHCCCCEEEHHHHHH
HLDGLAVLEKMRHIERLKQPNVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLTSHIR
CCCHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHH
QVSGKTNAAIKRPLPSFRSVTTADGKPKNLDASITSIIHEIGVPAHIKGYMYLREAISMV
HHCCCCCHHHHCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
YNDIELLGSITKVLYPDIAKKYNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTVSMS
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEECC
KAKPTNSEFIAMVADKLRLEHKAS
CCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEKIKVCLVDDNKELVSMLESYVAAQDDMEVIGTAYNGQECLNLLKEKQPDVLVLDIIMP
CCCEEEEEECCCHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHCCCCEEEHHHHHH
HLDGLAVLEKMRHIERLKQPNVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLTSHIR
CCCHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHH
QVSGKTNAAIKRPLPSFRSVTTADGKPKNLDASITSIIHEIGVPAHIKGYMYLREAISMV
HHCCCCCHHHHCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
YNDIELLGSITKVLYPDIAKKYNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTVSMS
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEECC
KAKPTNSEFIAMVADKLRLEHKAS
CCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Protein L-glutamate Omethyl ester; Tapglu-Me; H2O; Targlu-Me; Trgglu-Me; Tsrglu-Me; Tsrgln; Trggln; Targln; Tapgln; protein-L-glutamine [C]

Specific reaction: Protein L-glutamate Omethyl ester + H2O = protein L-glutamate+ methanol H2O + Tapglu-Me = methanol + Tapglu H2O + Targlu-Me = methanol + Targlu H2O + Trgglu-Me = methanol + Trgglu H2O + Tsrglu-Me = methanol + Tsrglu H2O + Tsrgln = NH3 + Tsrglu H2O + Trggl

General reaction: Hydrolysis of carboxylic ester [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629; 7885226 [H]