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The map label for this gene is recN [H]

Identifier: 152976578

GI number: 152976578

Start: 2940641

End: 2942380

Strand: Reverse

Name: recN [H]

Synonym: Bcer98_2867

Alternate gene names: 152976578

Gene position: 2942380-2940641 (Counterclockwise)

Preceding gene: 152976579

Following gene: 152976577

Centisome position: 71.99

GC content: 34.48

Gene sequence:

>1740_bases
TTGTTATCGGAATTATCGATTAGAAACTTTGCTATTATTGAGTCATTAAATATTTCTTTTCAAAAAGGCTTAACTGTATT
AAGTGGTGAAACAGGAGCTGGAAAATCAATTATTATTGATGCAATTAGCTTGCTTGTTGGTGGTCGTGGGTCAGCAGAAT
TTGTTCGATATGGAACAGAGAAAGCAGAAATTGAAGGATTGTTTTATATAGAGGACGATAAGCACCCGTGCATTGCGAAG
GCAGAAGAGCTGGATATTGAAATTGAAGATGGCATGATGATTTTAAAACGCGACATAGCTGCGAATGGAAAAAGTGTATG
TCGCATAAATGGTAAACTTGTTACATTAAGCATATTGAAAGAAATTGGGAAAACACTTGTTGATATTCACGGACAACATG
AGACACAAGATTTAATGAATGAAGAACGGCATCTCTTTATGCTGGATCATTTCGATGGAAATCGAATTGTAAAACAATTA
GAAAAATATCAAGAAGTATATGTAAAGTATGAACAATTGAAGAAACAATTAAAAGCTTTAACAGAAAATGAACAACAGAT
GGCTCACCGATTGGATTTAATTCAATTCCAATATGAAGAAATTCGTAAAGCGGATTTAAAGGTAGATGAAGAAGAGGAAT
TAACAGAGGAGCGCTTGAAAATCTCTAATTTTGAGAAGATTTATAAAGCGTTAGGTGATGCTTATCGTTCATTAACTGCA
GATGGACAAGGCCTTGATCATGTGCGAAATGCAATGGGGCAAATGGAGAGTATTGCACATTTAGATGAATCGTATCAAGA
AAATCATGATTCAATAGCGAATAGCTATTATTTGCTGGAAGAAGTAGCCTATCAGCTAAGAGAAAAATTAGATATGATGG
AATATGATCCAAATCGTTTAGATGAAATTGAAACTCGTTTAAATGAAATTCGTATGCTAAAGAGAAAGTATGGCAATACT
GTAGAAGAGATTTTAGCGTATGCAGATAAAATAGAGCAAGAAATATTTACGATTGAAAATAAAGATGTGCATATTGAAAA
GGCTAAAAAAGAGTTAAAAGAATTGGAAAGTGTAATCTTAAAAGAAGCGACAATGTTAAGTGATATGCGTTATGAACTTG
CAGAGCGCTTAACAAGTGCTATTCACCAAGAATTGAAAGAACTATATATGGAAAAAACAAAATTTGAAGTGAGAATAACA
AAAAAAGAAGGAAATATAGAAGATCCACTCGTTGAAGGAATACCTGTCAAATTAACAGTGGATGGATATGACCAAGTGGA
GTTCTATATTTCCACAAATCCAGGTGAACCGTTGAAACAACTTTCAAAAGTTGCTTCTGGAGGAGAGTTATCTCGTATTA
TTTTAGCTTTAAAGAGTATTTTTTCGAAACATCATGGTGTCACATCTGTCATTTTTGATGAAGTGGATACAGGTGTGAGC
GGCCGAGTTGCACAAGCGATTGCTGAAAAAATTTACAGGGTATCAGTAAATTCACAAGTACTTTGCATTACTCATTTACC
TCAAGTAGCTTCAATGGCGGATTCACATTTATTTATCCGAAAGCAAGTGGCGAATGATCGGACAATGACATCTGTCACAA
TACTAAGTCCAGAAGATAAAGTGACAGAAATTGCTCGTATGATTTCTGGTGTAGAAATTACGGATTTAACTACGGAACAT
GCGAAAGAGTTACTTACGCAAGCAAATCATTTTAAGCAAGCAGCAGAAGCTATCCAATAA

Upstream 100 bases:

>100_bases
AGAATACATGATACATGTGATATATAGAAGATAACGATTTAAATAATACGTAATGAAAGCTCTTAATGGGGCATTAGTTT
ATAACGAGGTGAATGAAGCA

Downstream 100 bases:

>100_bases
TGGATAGCTTTTTTGTTCAAATAATGATTTCTTAATTCTTCCGGTTATAAACAGGGCGTTGCAAGGAGAGATTAAAAGAT
GAGGCCGAAGAGGCTGAATG

Product: DNA repair protein RecN

Products: NA

Alternate protein names: Recombination protein N [H]

Number of amino acids: Translated: 579; Mature: 579

Protein sequence:

>579_residues
MLSELSIRNFAIIESLNISFQKGLTVLSGETGAGKSIIIDAISLLVGGRGSAEFVRYGTEKAEIEGLFYIEDDKHPCIAK
AEELDIEIEDGMMILKRDIAANGKSVCRINGKLVTLSILKEIGKTLVDIHGQHETQDLMNEERHLFMLDHFDGNRIVKQL
EKYQEVYVKYEQLKKQLKALTENEQQMAHRLDLIQFQYEEIRKADLKVDEEEELTEERLKISNFEKIYKALGDAYRSLTA
DGQGLDHVRNAMGQMESIAHLDESYQENHDSIANSYYLLEEVAYQLREKLDMMEYDPNRLDEIETRLNEIRMLKRKYGNT
VEEILAYADKIEQEIFTIENKDVHIEKAKKELKELESVILKEATMLSDMRYELAERLTSAIHQELKELYMEKTKFEVRIT
KKEGNIEDPLVEGIPVKLTVDGYDQVEFYISTNPGEPLKQLSKVASGGELSRIILALKSIFSKHHGVTSVIFDEVDTGVS
GRVAQAIAEKIYRVSVNSQVLCITHLPQVASMADSHLFIRKQVANDRTMTSVTILSPEDKVTEIARMISGVEITDLTTEH
AKELLTQANHFKQAAEAIQ

Sequences:

>Translated_579_residues
MLSELSIRNFAIIESLNISFQKGLTVLSGETGAGKSIIIDAISLLVGGRGSAEFVRYGTEKAEIEGLFYIEDDKHPCIAK
AEELDIEIEDGMMILKRDIAANGKSVCRINGKLVTLSILKEIGKTLVDIHGQHETQDLMNEERHLFMLDHFDGNRIVKQL
EKYQEVYVKYEQLKKQLKALTENEQQMAHRLDLIQFQYEEIRKADLKVDEEEELTEERLKISNFEKIYKALGDAYRSLTA
DGQGLDHVRNAMGQMESIAHLDESYQENHDSIANSYYLLEEVAYQLREKLDMMEYDPNRLDEIETRLNEIRMLKRKYGNT
VEEILAYADKIEQEIFTIENKDVHIEKAKKELKELESVILKEATMLSDMRYELAERLTSAIHQELKELYMEKTKFEVRIT
KKEGNIEDPLVEGIPVKLTVDGYDQVEFYISTNPGEPLKQLSKVASGGELSRIILALKSIFSKHHGVTSVIFDEVDTGVS
GRVAQAIAEKIYRVSVNSQVLCITHLPQVASMADSHLFIRKQVANDRTMTSVTILSPEDKVTEIARMISGVEITDLTTEH
AKELLTQANHFKQAAEAIQ
>Mature_579_residues
MLSELSIRNFAIIESLNISFQKGLTVLSGETGAGKSIIIDAISLLVGGRGSAEFVRYGTEKAEIEGLFYIEDDKHPCIAK
AEELDIEIEDGMMILKRDIAANGKSVCRINGKLVTLSILKEIGKTLVDIHGQHETQDLMNEERHLFMLDHFDGNRIVKQL
EKYQEVYVKYEQLKKQLKALTENEQQMAHRLDLIQFQYEEIRKADLKVDEEEELTEERLKISNFEKIYKALGDAYRSLTA
DGQGLDHVRNAMGQMESIAHLDESYQENHDSIANSYYLLEEVAYQLREKLDMMEYDPNRLDEIETRLNEIRMLKRKYGNT
VEEILAYADKIEQEIFTIENKDVHIEKAKKELKELESVILKEATMLSDMRYELAERLTSAIHQELKELYMEKTKFEVRIT
KKEGNIEDPLVEGIPVKLTVDGYDQVEFYISTNPGEPLKQLSKVASGGELSRIILALKSIFSKHHGVTSVIFDEVDTGVS
GRVAQAIAEKIYRVSVNSQVLCITHLPQVASMADSHLFIRKQVANDRTMTSVTILSPEDKVTEIARMISGVEITDLTTEH
AKELLTQANHFKQAAEAIQ

Specific function: Involved in recombinational repair of damaged DNA. Seems to be the first protein recruited to repair centers, foci that are the site of double-strand DNA break(s), followed by recO and then recF [H]

COG id: COG0497

COG function: function code L; ATPase involved in DNA repair

Gene ontology:

Cell location: Cytoplasm, nucleoid. Note=Cytoplasmically located in untreated cells. Recruited to foci following treatment with DNA damaging agents; these foci are presumably the breaks themselves. They are almost always located within nucleoids [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recN family [H]

Homologues:

Organism=Escherichia coli, GI48994901, Length=573, Percent_Identity=32.4607329842932, Blast_Score=294, Evalue=1e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004604
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 65965; Mature: 65965

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSELSIRNFAIIESLNISFQKGLTVLSGETGAGKSIIIDAISLLVGGRGSAEFVRYGTE
CCCCCCCCCCHHHHHCCCHHHHCCEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHCCCC
KAEIEGLFYIEDDKHPCIAKAEELDIEIEDGMMILKRDIAANGKSVCRINGKLVTLSILK
HHHCCEEEEEECCCCCCCCCHHHCCEEECCCEEEEHHHHCCCCCCEEEECCEEEHHHHHH
EIGKTLVDIHGQHETQDLMNEERHLFMLDHFDGNRIVKQLEKYQEVYVKYEQLKKQLKAL
HHHHHHHHHCCCCHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
TENEQQMAHRLDLIQFQYEEIRKADLKVDEEEELTEERLKISNFEKIYKALGDAYRSLTA
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DGQGLDHVRNAMGQMESIAHLDESYQENHDSIANSYYLLEEVAYQLREKLDMMEYDPNRL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
DEIETRLNEIRMLKRKYGNTVEEILAYADKIEQEIFTIENKDVHIEKAKKELKELESVIL
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHH
KEATMLSDMRYELAERLTSAIHQELKELYMEKTKFEVRITKKEGNIEDPLVEGIPVKLTV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCHHCCCCEEEEE
DGYDQVEFYISTNPGEPLKQLSKVASGGELSRIILALKSIFSKHHGVTSVIFDEVDTGVS
CCCCEEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCC
GRVAQAIAEKIYRVSVNSQVLCITHLPQVASMADSHLFIRKQVANDRTMTSVTILSPEDK
HHHHHHHHHHHHHEECCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHH
VTEIARMISGVEITDLTTEHAKELLTQANHFKQAAEAIQ
HHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLSELSIRNFAIIESLNISFQKGLTVLSGETGAGKSIIIDAISLLVGGRGSAEFVRYGTE
CCCCCCCCCCHHHHHCCCHHHHCCEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHCCCC
KAEIEGLFYIEDDKHPCIAKAEELDIEIEDGMMILKRDIAANGKSVCRINGKLVTLSILK
HHHCCEEEEEECCCCCCCCCHHHCCEEECCCEEEEHHHHCCCCCCEEEECCEEEHHHHHH
EIGKTLVDIHGQHETQDLMNEERHLFMLDHFDGNRIVKQLEKYQEVYVKYEQLKKQLKAL
HHHHHHHHHCCCCHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
TENEQQMAHRLDLIQFQYEEIRKADLKVDEEEELTEERLKISNFEKIYKALGDAYRSLTA
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DGQGLDHVRNAMGQMESIAHLDESYQENHDSIANSYYLLEEVAYQLREKLDMMEYDPNRL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
DEIETRLNEIRMLKRKYGNTVEEILAYADKIEQEIFTIENKDVHIEKAKKELKELESVIL
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHH
KEATMLSDMRYELAERLTSAIHQELKELYMEKTKFEVRITKKEGNIEDPLVEGIPVKLTV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCHHCCCCEEEEE
DGYDQVEFYISTNPGEPLKQLSKVASGGELSRIILALKSIFSKHHGVTSVIFDEVDTGVS
CCCCEEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCC
GRVAQAIAEKIYRVSVNSQVLCITHLPQVASMADSHLFIRKQVANDRTMTSVTILSPEDK
HHHHHHHHHHHHHEECCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHH
VTEIARMISGVEITDLTTEHAKELLTQANHFKQAAEAIQ
HHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2106508; 8969508; 9384377; 2507400; 9044256 [H]