BacMap

Definition	Bacillus cereus subsp. cytotoxis NVH 391-98, complete genome.
Accession	NC_009674
Length	4,087,024

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The map label for this gene is kbl [C]

Identifier: 152976527

GI number: 152976527

Start: 2891002

End: 2892189

Strand: Reverse

Name: kbl [C]

Synonym: Bcer98_2816

Alternate gene names: 152976527

Gene position: 2892189-2891002 (Counterclockwise)

Preceding gene: 152976528

Following gene: 152976526

Centisome position: 70.77

GC content: 40.66

Gene sequence:

>1188_bases
ATGGAACAAGCATGGAATTTACACTTTTACGCTAGTTTAAATCGTCTAAAAGAAAAGTCACAATACCGTGAAGTAGTGGT
AACGAATCAAGCTGAAGAACCGTGGCTGATTCGGAACGGAGCGCGTATGCTGAATTTGGCATCGAATAATTATCTCGGTT
TAGCGGGGGATAGTCGGTTAAAAGAAGCGGCTATTGAAGCGATAAGGACATATGGAGTAGGAGCGACGGCATCACGGCTT
GTGGTAGGAAACTATTCCCTTTATGAAGAGGTAGAAAAGAGTATTTGCGATTGGAAGCATACAGAGAAAGCGATGATTGT
GAACAGTGGATATACAGCGAATGTTGGAGCCATTTCTTCTCTAATGGGACGCCATGATGTCATTTTCAGTGATAAATTAA
ATCATGCTAGTATTGTTGACGGAGTGAAACTGAGCGGTGCAAAGCAGAAACGATATCGTCATAATGATTTACATCACTTG
GAGCAGCTACTACAAACAACACCTTCTGAAAAGAGAAAGTTAATTGTAACAGATACGGTGTTTAGTATGGAGGGAGATGT
TGCTCATTTAAAAGAATTGATTGCATTGAAAGAGAAATATGGAGCACTTCTTATGATTGATGAAGCACATGCCAGTGGCA
TATACGGGGAACGCGGTGCTGGAATTGCGCATGTGGAGCCAGAGCTTTCTAAAAAAATTGATATTCATATGGGTACATTT
AGTAAAGCGTTAGGGTGTTATGGGGCTTATTTAGCAGGAAATGAAGTTTGCATTGATTATATGAGAAACAAGATGAGAAG
TTTTATTTTTACAACGGCATTGCCACCAGGGGTACTTGCCACTATACAGTGTGCAATTGCTATTGTGAAAGAAGACGAGG
CAAGACGAAAAAGACTTTTAGAAAACGGTGCGTATTTTCGTAATGTGTTAAAAGAAGCTGGATATAACATTGGCAATAGT
TCAACACATATTGTTCCGGTTATTGTAACGTCGAATGAAGCGGCGATACGCTTTAGTAGAAAAGTGCAAGAAGCTGGAAT
TGTAGCGATTGCAATTCGGCCACCAACTGTTCCGCATAATAGCTCGCGCATTCGATTTACAGTGACATCTGAGCATACAA
AGTCTGATTTAGAGTGGGCGATTCGGCAAATTGTGAACATTGGAAAAGAAGAGGGGTTTGTGGAATGA

Upstream 100 bases:

>100_bases
GGTTTATTTCCAAAGATTCGGGAAGAATATACGCGCGATGAACTAATAAAAGCAGCAGAAGAGAGTATTCAGATTTCATA
TTTAGAGGAGTATATGAAAA

Downstream 100 bases:

>100_bases
AACAACTTAAGCTAATCTTTATCCCTGGATGGGGAATGGAAAAACAAGTATGGAGCCCAATCCTTTCACTTTTGCAGGAA
AGTTTTGCCGAGTATATTGA

Product: 8-amino-7-oxononanoate synthase

Products: NA

Alternate protein names: AONS/AKB ligase; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; Alpha-oxoamine synthase; L-alanine--pimeloyl-CoA ligase

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MEQAWNLHFYASLNRLKEKSQYREVVVTNQAEEPWLIRNGARMLNLASNNYLGLAGDSRLKEAAIEAIRTYGVGATASRL
VVGNYSLYEEVEKSICDWKHTEKAMIVNSGYTANVGAISSLMGRHDVIFSDKLNHASIVDGVKLSGAKQKRYRHNDLHHL
EQLLQTTPSEKRKLIVTDTVFSMEGDVAHLKELIALKEKYGALLMIDEAHASGIYGERGAGIAHVEPELSKKIDIHMGTF
SKALGCYGAYLAGNEVCIDYMRNKMRSFIFTTALPPGVLATIQCAIAIVKEDEARRKRLLENGAYFRNVLKEAGYNIGNS
STHIVPVIVTSNEAAIRFSRKVQEAGIVAIAIRPPTVPHNSSRIRFTVTSEHTKSDLEWAIRQIVNIGKEEGFVE

Sequences:

>Translated_395_residues
MEQAWNLHFYASLNRLKEKSQYREVVVTNQAEEPWLIRNGARMLNLASNNYLGLAGDSRLKEAAIEAIRTYGVGATASRL
VVGNYSLYEEVEKSICDWKHTEKAMIVNSGYTANVGAISSLMGRHDVIFSDKLNHASIVDGVKLSGAKQKRYRHNDLHHL
EQLLQTTPSEKRKLIVTDTVFSMEGDVAHLKELIALKEKYGALLMIDEAHASGIYGERGAGIAHVEPELSKKIDIHMGTF
SKALGCYGAYLAGNEVCIDYMRNKMRSFIFTTALPPGVLATIQCAIAIVKEDEARRKRLLENGAYFRNVLKEAGYNIGNS
STHIVPVIVTSNEAAIRFSRKVQEAGIVAIAIRPPTVPHNSSRIRFTVTSEHTKSDLEWAIRQIVNIGKEEGFVE
>Mature_395_residues
MEQAWNLHFYASLNRLKEKSQYREVVVTNQAEEPWLIRNGARMLNLASNNYLGLAGDSRLKEAAIEAIRTYGVGATASRL
VVGNYSLYEEVEKSICDWKHTEKAMIVNSGYTANVGAISSLMGRHDVIFSDKLNHASIVDGVKLSGAKQKRYRHNDLHHL
EQLLQTTPSEKRKLIVTDTVFSMEGDVAHLKELIALKEKYGALLMIDEAHASGIYGERGAGIAHVEPELSKKIDIHMGTF
SKALGCYGAYLAGNEVCIDYMRNKMRSFIFTTALPPGVLATIQCAIAIVKEDEARRKRLLENGAYFRNVLKEAGYNIGNS
STHIVPVIVTSNEAAIRFSRKVQEAGIVAIAIRPPTVPHNSSRIRFTVTSEHTKSDLEWAIRQIVNIGKEEGFVE

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A and/or converts 2-amino-3-ketobutyrate to glycine and acetyl-CoA

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family

Homologues:

Organism=Homo sapiens, GI7657118, Length=351, Percent_Identity=32.1937321937322, Blast_Score=223, Evalue=4e-58,
Organism=Homo sapiens, GI284448556, Length=351, Percent_Identity=32.1937321937322, Blast_Score=222, Evalue=4e-58,
Organism=Homo sapiens, GI4758668, Length=366, Percent_Identity=34.4262295081967, Blast_Score=216, Evalue=4e-56,
Organism=Homo sapiens, GI119220554, Length=376, Percent_Identity=31.3829787234043, Blast_Score=201, Evalue=1e-51,
Organism=Homo sapiens, GI83977442, Length=344, Percent_Identity=32.2674418604651, Blast_Score=193, Evalue=2e-49,
Organism=Homo sapiens, GI83977444, Length=344, Percent_Identity=32.2674418604651, Blast_Score=193, Evalue=3e-49,
Organism=Homo sapiens, GI83977440, Length=344, Percent_Identity=32.2674418604651, Blast_Score=192, Evalue=3e-49,
Organism=Homo sapiens, GI4502025, Length=352, Percent_Identity=32.3863636363636, Blast_Score=185, Evalue=7e-47,
Organism=Homo sapiens, GI40316939, Length=352, Percent_Identity=32.3863636363636, Blast_Score=185, Evalue=7e-47,
Organism=Homo sapiens, GI5454084, Length=250, Percent_Identity=30, Blast_Score=134, Evalue=2e-31,
Organism=Escherichia coli, GI1790046, Length=374, Percent_Identity=33.6898395721925, Blast_Score=235, Evalue=3e-63,
Organism=Escherichia coli, GI1786993, Length=349, Percent_Identity=32.378223495702, Blast_Score=217, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI71994529, Length=351, Percent_Identity=35.042735042735, Blast_Score=229, Evalue=2e-60,
Organism=Caenorhabditis elegans, GI32566772, Length=369, Percent_Identity=33.0623306233062, Blast_Score=209, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI17560912, Length=363, Percent_Identity=29.4765840220386, Blast_Score=181, Evalue=5e-46,
Organism=Caenorhabditis elegans, GI17560914, Length=363, Percent_Identity=29.4765840220386, Blast_Score=181, Evalue=5e-46,
Organism=Caenorhabditis elegans, GI71982617, Length=285, Percent_Identity=25.9649122807018, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI71982625, Length=285, Percent_Identity=25.9649122807018, Blast_Score=125, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6320267, Length=364, Percent_Identity=29.6703296703297, Blast_Score=195, Evalue=8e-51,
Organism=Saccharomyces cerevisiae, GI6320438, Length=378, Percent_Identity=33.0687830687831, Blast_Score=194, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6323954, Length=243, Percent_Identity=30.4526748971193, Blast_Score=127, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24662918, Length=366, Percent_Identity=32.5136612021858, Blast_Score=237, Evalue=9e-63,
Organism=Drosophila melanogaster, GI17137420, Length=332, Percent_Identity=34.3373493975904, Blast_Score=196, Evalue=2e-50,
Organism=Drosophila melanogaster, GI17136286, Length=373, Percent_Identity=32.9758713136729, Blast_Score=195, Evalue=4e-50,
Organism=Drosophila melanogaster, GI24653280, Length=248, Percent_Identity=27.8225806451613, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24653276, Length=248, Percent_Identity=27.8225806451613, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24653278, Length=248, Percent_Identity=27.8225806451613, Blast_Score=119, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIKB_BACCN (A7GSE1)

Other databases:

- EMBL:   CP000764
- RefSeq:   YP_001376044.1
- ProteinModelPortal:   A7GSE1
- SMR:   A7GSE1
- STRING:   A7GSE1
- EnsemblBacteria:   EBBACT00000036123
- GeneID:   5343870
- GenomeReviews:   CP000764_GR
- KEGG:   bcy:Bcer98_2816
- eggNOG:   COG0156
- GeneTree:   EBGT00050000000589
- HOGENOM:   HBG649839
- OMA:   IDEAHAS
- ProtClustDB:   PRK05958
- BioCyc:   BCER315749:BCER98_2816-MONOMER
- InterPro:   IPR001917
- InterPro:   IPR004839
- InterPro:   IPR004723
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- TIGRFAMs:   TIGR00858

Pfam domain/function: PF00155 Aminotran_1_2; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.3.1.29; =2.3.1.47

Molecular weight: Translated: 43959; Mature: 43959

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: BINDING 23-23 BINDING 135-135 BINDING 182-182 BINDING 356-356

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQAWNLHFYASLNRLKEKSQYREVVVTNQAEEPWLIRNGARMLNLASNNYLGLAGDSRL
CCCCEEEEHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCCEEEEECCCCEEEECCCHHH
KEAAIEAIRTYGVGATASRLVVGNYSLYEEVEKSICDWKHTEKAMIVNSGYTANVGAISS
HHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHH
LMGRHDVIFSDKLNHASIVDGVKLSGAKQKRYRHNDLHHLEQLLQTTPSEKRKLIVTDTV
HHCCCCEEEECCCCCCHHHCCCCCCCCHHHHHHCCHHHHHHHHHHCCCCCCCEEEEEEEE
FSMEGDVAHLKELIALKEKYGALLMIDEAHASGIYGERGAGIAHVEPELSKKIDIHMGTF
ECCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCEEEECHHHHCEEEEEECHH
SKALGCYGAYLAGNEVCIDYMRNKMRSFIFTTALPPGVLATIQCAIAIVKEDEARRKRLL
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEECCCHHHHHHHH
ENGAYFRNVLKEAGYNIGNSSTHIVPVIVTSNEAAIRFSRKVQEAGIVAIAIRPPTVPHN
HCCHHHHHHHHHCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHCCEEEEEEECCCCCCC
SSRIRFTVTSEHTKSDLEWAIRQIVNIGKEEGFVE
CCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MEQAWNLHFYASLNRLKEKSQYREVVVTNQAEEPWLIRNGARMLNLASNNYLGLAGDSRL
CCCCEEEEHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCCEEEEECCCCEEEECCCHHH
KEAAIEAIRTYGVGATASRLVVGNYSLYEEVEKSICDWKHTEKAMIVNSGYTANVGAISS
HHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHH
LMGRHDVIFSDKLNHASIVDGVKLSGAKQKRYRHNDLHHLEQLLQTTPSEKRKLIVTDTV
HHCCCCEEEECCCCCCHHHCCCCCCCCHHHHHHCCHHHHHHHHHHCCCCCCCEEEEEEEE
FSMEGDVAHLKELIALKEKYGALLMIDEAHASGIYGERGAGIAHVEPELSKKIDIHMGTF
ECCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCEEEECHHHHCEEEEEECHH
SKALGCYGAYLAGNEVCIDYMRNKMRSFIFTTALPPGVLATIQCAIAIVKEDEARRKRLL
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEECCCHHHHHHHH
ENGAYFRNVLKEAGYNIGNSSTHIVPVIVTSNEAAIRFSRKVQEAGIVAIAIRPPTVPHN
HCCHHHHHHHHHCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHCCEEEEEEECCCCCCC
SSRIRFTVTSEHTKSDLEWAIRQIVNIGKEEGFVE
CCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA