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The map label for this gene is bioH [H]

Identifier: 152976526

GI number: 152976526

Start: 2890289

End: 2891005

Strand: Reverse

Name: bioH [H]

Synonym: Bcer98_2815

Alternate gene names: 152976526

Gene position: 2891005-2890289 (Counterclockwise)

Preceding gene: 152976527

Following gene: 152976525

Centisome position: 70.74

GC content: 35.43

Gene sequence:

>717_bases
ATGAAACAACTTAAGCTAATCTTTATCCCTGGATGGGGAATGGAAAAACAAGTATGGAGCCCAATCCTTTCACTTTTGCA
GGAAAGTTTTGCCGAGTATATTGAGTGGCATGATGTAAAAGAAATCAATGAATTTGCAAAGCGGGTAGAAAAAGCTGCTG
AAGGATACGATGTCATTTTAATCGGGTGGTCGCTTGGCGCGTTAGTTGCCCTTGAAGTATGTAACAATGTGAAAACAAAG
GGAATGATTTTAATAAGTAGTACAGCAAAGTTTACAGTAGATGAAAGGTATAAACATGGCTGGAAACCTTCTTTTGTTGA
GAGAATGAAAAGGAATATAAAAAAAAGAAAGAATGAGACATTGAGCCGTTTTTATATAAGTATGTTTGCCGAAGAAGAGA
GTGCTGTAAAAGAATCATTTGAAAATATGATAAAAGAATTTCAAGGTGATTCGACAGAGTCTTTACAAACAGGGCTCGAT
TATTTAATGAGAGCGGACATGAGGGAACGCTTAAAAGATGTAAATGTACCAATGTTACTTTTACATGGAGAACAAGATTC
CATTTGTCCTTTGCCTGCTGCTCATTACATAAAAGAAAAGACAAATGCAACACTTAAAGTGATTCATCAAGCGGGGCATG
CCATTTGTATAACGAATTTTGAATATTGCGCAAATGAAATTAATAAATTCATAGAGGGGATACGAAATGATCAATAA

Upstream 100 bases:

>100_bases
CGCGCATTCGATTTACAGTGACATCTGAGCATACAAAGTCTGATTTAGAGTGGGCGATTCGGCAAATTGTGAACATTGGA
AAAGAAGAGGGGTTTGTGGA

Downstream 100 bases:

>100_bases
AACATTGTTGCAAAAACGATTTAATAGAGCGGCTGTATCCTATGATCAATATGCCAATGTACAAAAGAAAATGGCTCGTC
ATTTACTTTCGCAGTTGGAA

Product: carboxylesterase

Products: NA

Alternate protein names: Biotin synthesis protein BioH [H]

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MKQLKLIFIPGWGMEKQVWSPILSLLQESFAEYIEWHDVKEINEFAKRVEKAAEGYDVILIGWSLGALVALEVCNNVKTK
GMILISSTAKFTVDERYKHGWKPSFVERMKRNIKKRKNETLSRFYISMFAEEESAVKESFENMIKEFQGDSTESLQTGLD
YLMRADMRERLKDVNVPMLLLHGEQDSICPLPAAHYIKEKTNATLKVIHQAGHAICITNFEYCANEINKFIEGIRNDQ

Sequences:

>Translated_238_residues
MKQLKLIFIPGWGMEKQVWSPILSLLQESFAEYIEWHDVKEINEFAKRVEKAAEGYDVILIGWSLGALVALEVCNNVKTK
GMILISSTAKFTVDERYKHGWKPSFVERMKRNIKKRKNETLSRFYISMFAEEESAVKESFENMIKEFQGDSTESLQTGLD
YLMRADMRERLKDVNVPMLLLHGEQDSICPLPAAHYIKEKTNATLKVIHQAGHAICITNFEYCANEINKFIEGIRNDQ
>Mature_238_residues
MKQLKLIFIPGWGMEKQVWSPILSLLQESFAEYIEWHDVKEINEFAKRVEKAAEGYDVILIGWSLGALVALEVCNNVKTK
GMILISSTAKFTVDERYKHGWKPSFVERMKRNIKKRKNETLSRFYISMFAEEESAVKESFENMIKEFQGDSTESLQTGLD
YLMRADMRERLKDVNVPMLLLHGEQDSICPLPAAHYIKEKTNATLKVIHQAGHAICITNFEYCANEINKFIEGIRNDQ

Specific function: Shows carboxylesterase activity with a preference for short chain fatty acid esters (acyl chain length of up to 6 carbons). Also displays a weak thioesterase activity. Can form a complex with CoA, and may be involved in the condensation of CoA and pimelic

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family [H]

Homologues:

Organism=Escherichia coli, GI1789817, Length=235, Percent_Identity=22.1276595744681, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010076 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 27515; Mature: 27515

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00120 LIPASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQLKLIFIPGWGMEKQVWSPILSLLQESFAEYIEWHDVKEINEFAKRVEKAAEGYDVIL
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEE
IGWSLGALVALEVCNNVKTKGMILISSTAKFTVDERYKHGWKPSFVERMKRNIKKRKNET
ECCHHHHHHHHHHHHCCCCCCEEEEECCCCEEHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LSRFYISMFAEEESAVKESFENMIKEFQGDSTESLQTGLDYLMRADMRERLKDVNVPMLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LHGEQDSICPLPAAHYIKEKTNATLKVIHQAGHAICITNFEYCANEINKFIEGIRNDQ
EECCCCCCCCCCHHHHHHHHCCHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKQLKLIFIPGWGMEKQVWSPILSLLQESFAEYIEWHDVKEINEFAKRVEKAAEGYDVIL
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEE
IGWSLGALVALEVCNNVKTKGMILISSTAKFTVDERYKHGWKPSFVERMKRNIKKRKNET
ECCHHHHHHHHHHHHCCCCCCEEEEECCCCEEHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LSRFYISMFAEEESAVKESFENMIKEFQGDSTESLQTGLDYLMRADMRERLKDVNVPMLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LHGEQDSICPLPAAHYIKEKTNATLKVIHQAGHAICITNFEYCANEINKFIEGIRNDQ
EECCCCCCCCCCHHHHHHHHCCHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA