Click here to switch to the map view.

The map label for this gene is hpaG [H]

Identifier: 152974780

GI number: 152974780

Start: 1092955

End: 1093746

Strand: Direct

Name: hpaG [H]

Synonym: Bcer98_0971

Alternate gene names: 152974780

Gene position: 1092955-1093746 (Clockwise)

Preceding gene: 152974779

Following gene: 152974781

Centisome position: 26.74

GC content: 40.15

Gene sequence:

>792_bases
ATGAGGAAAATACAGTTTAAATGTTATGGCCGATCCCAAATAGAAGAAGCGGAGCTACATATAACAGAAGATATGGTCAT
ATGGAACGGAAAAGAGTATAAAAGTCACGAGCTTGCATTGGATATTCCAACTTCAGGAAATATTTACGGGACATTGCTCA
ATTATAAAGGAGCACTTGCCGCATTAGGAAATTCGGTACATGAATTGCCATATAAACAAGCGCCAATGGCTCCCATTTTA
TATATCAAACCGATAAACACAATGATTGCCCGGGGAATGCCGATTCCGTTACCGAGTGAAGAAAGGGAGTTAGAAGTTGG
GGCAGCACTAGGAATTGTCATCGGAAAAAGAGCGACGAAAGTAAGGGAAGAAGAAGCGTTAACATATATTCAAGGATATA
CGATTGTAAATGACATCAGCATACCTCATGAAAGCGTGTATCGCCCAGCGATTAAGCAAAAAGCACGCGATGGATTTTGT
CCAGTTGGCCCATGGGTGATAGAGAAAGGGGCTATTCAAAACCCAAATGATGTAAGCATTCAAGTATATGTGAACGGTAT
ATTGCGGCAAGAAAATCATACGAAAAACTTAATTAGACCAGTGGAACGACTTATCGCAGATGTAACAGAATTTATGACTT
TATATGAAGGAGATATACTGCTTGTTGGTGTCCCGGAAAATCCACCACTCGTAAAAAATGGAGACCGCATTCGAATTGAA
ATCGAAGGAATTGGCAGCTTAGAAAATCAAGTCGTTTTAGAAAAAGAACTTGTGAGAGGAGGAGTACGATGA

Upstream 100 bases:

>100_bases
GAAGTAAGACGGGTAATTTTGAAGAAATATTGGTGAAGTCGAATACAGGAAGCAATGTGAAAGGTATTAGTCATCATTAA
GTGAAATGAGGGAGGAAAAT

Downstream 100 bases:

>100_bases
AACGAGCGCGCATCGCATATGCTGGTGGGATTTACTACGCAACTGAAGTAGATGGCAAGTTGCAATTAGATGATGGACGG
ATGCTAGTAGAAGAGGAAGT

Product: 4-hydroxyphenylacetate degradation bifunctional isomerase/decarboxylase, HpaG1 subunit

Products: NA

Alternate protein names: 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase; HHDD isomerase; 5-carboxymethyl-2-hydroxymuconate Delta-isomerase; 5-carboxymethyl-2-oxo-hex-3-ene-1,7-dioate decarboxylase; 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase; OPET decarboxylase [H]

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MRKIQFKCYGRSQIEEAELHITEDMVIWNGKEYKSHELALDIPTSGNIYGTLLNYKGALAALGNSVHELPYKQAPMAPIL
YIKPINTMIARGMPIPLPSEERELEVGAALGIVIGKRATKVREEEALTYIQGYTIVNDISIPHESVYRPAIKQKARDGFC
PVGPWVIEKGAIQNPNDVSIQVYVNGILRQENHTKNLIRPVERLIADVTEFMTLYEGDILLVGVPENPPLVKNGDRIRIE
IEGIGSLENQVVLEKELVRGGVR

Sequences:

>Translated_263_residues
MRKIQFKCYGRSQIEEAELHITEDMVIWNGKEYKSHELALDIPTSGNIYGTLLNYKGALAALGNSVHELPYKQAPMAPIL
YIKPINTMIARGMPIPLPSEERELEVGAALGIVIGKRATKVREEEALTYIQGYTIVNDISIPHESVYRPAIKQKARDGFC
PVGPWVIEKGAIQNPNDVSIQVYVNGILRQENHTKNLIRPVERLIADVTEFMTLYEGDILLVGVPENPPLVKNGDRIRIE
IEGIGSLENQVVLEKELVRGGVR
>Mature_263_residues
MRKIQFKCYGRSQIEEAELHITEDMVIWNGKEYKSHELALDIPTSGNIYGTLLNYKGALAALGNSVHELPYKQAPMAPIL
YIKPINTMIARGMPIPLPSEERELEVGAALGIVIGKRATKVREEEALTYIQGYTIVNDISIPHESVYRPAIKQKARDGFC
PVGPWVIEKGAIQNPNDVSIQVYVNGILRQENHTKNLIRPVERLIADVTEFMTLYEGDILLVGVPENPPLVKNGDRIRIE
IEGIGSLENQVVLEKELVRGGVR

Specific function: Decarboxylates OPET (5-oxo-pent-3-ene-1,2,5- tricarboxylic acid) into HHDD (2-hydroxy-hept-2,4-diene-1,7- dioate) and isomerizes it to OHED (2-oxo-hept-3-ene-1,7-dioate) [H]

COG id: COG0179

COG function: function code Q; 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAH family [H]

Homologues:

Organism=Homo sapiens, GI40786394, Length=189, Percent_Identity=31.7460317460317, Blast_Score=110, Evalue=9e-25,
Organism=Homo sapiens, GI156231349, Length=188, Percent_Identity=30.8510638297872, Blast_Score=109, Evalue=2e-24,
Organism=Homo sapiens, GI13654274, Length=179, Percent_Identity=34.0782122905028, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI66348062, Length=172, Percent_Identity=34.3023255813954, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI215422413, Length=172, Percent_Identity=34.3023255813954, Blast_Score=95, Evalue=6e-20,
Organism=Escherichia coli, GI1787428, Length=209, Percent_Identity=26.3157894736842, Blast_Score=87, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17557057, Length=191, Percent_Identity=31.413612565445, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI28571789, Length=210, Percent_Identity=30, Blast_Score=94, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24663695, Length=185, Percent_Identity=29.7297297297297, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI28572127, Length=191, Percent_Identity=28.2722513089005, Blast_Score=89, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234
- InterPro:   IPR012684
- InterPro:   IPR012686 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: =5.3.3.10; =4.1.1.68 [H]

Molecular weight: Translated: 29405; Mature: 29405

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKIQFKCYGRSQIEEAELHITEDMVIWNGKEYKSHELALDIPTSGNIYGTLLNYKGALA
CCEEEEEECCCCCCCCCCEEEEEEEEEECCCCCCCCEEEEECCCCCCEEEEEECCCCHHH
ALGNSVHELPYKQAPMAPILYIKPINTMIARGMPIPLPSEERELEVGAALGIVIGKRATK
HHCCHHHHCCCCCCCCCCEEEEECHHHHHHCCCCCCCCCCCCCEEHHEEEEEEECCHHHH
VREEEALTYIQGYTIVNDISIPHESVYRPAIKQKARDGFCPVGPWVIEKGAIQNPNDVSI
HHHHHHHEEEECEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCCCCCCEE
QVYVNGILRQENHTKNLIRPVERLIADVTEFMTLYEGDILLVGVPENPPLVKNGDRIRIE
EEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEEEEE
IEGIGSLENQVVLEKELVRGGVR
EECCCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRKIQFKCYGRSQIEEAELHITEDMVIWNGKEYKSHELALDIPTSGNIYGTLLNYKGALA
CCEEEEEECCCCCCCCCCEEEEEEEEEECCCCCCCCEEEEECCCCCCEEEEEECCCCHHH
ALGNSVHELPYKQAPMAPILYIKPINTMIARGMPIPLPSEERELEVGAALGIVIGKRATK
HHCCHHHHCCCCCCCCCCEEEEECHHHHHHCCCCCCCCCCCCCEEHHEEEEEEECCHHHH
VREEEALTYIQGYTIVNDISIPHESVYRPAIKQKARDGFCPVGPWVIEKGAIQNPNDVSI
HHHHHHHEEEECEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCCCCCCEE
QVYVNGILRQENHTKNLIRPVERLIADVTEFMTLYEGDILLVGVPENPPLVKNGDRIRIE
EEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEEEEE
IEGIGSLENQVVLEKELVRGGVR
EECCCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550403 [H]