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The map label for this gene is hpaG [H]

Identifier: 152974781

GI number: 152974781

Start: 1093743

End: 1094501

Strand: Direct

Name: hpaG [H]

Synonym: Bcer98_0972

Alternate gene names: 152974781

Gene position: 1093743-1094501 (Clockwise)

Preceding gene: 152974780

Following gene: 152974782

Centisome position: 26.76

GC content: 43.74

Gene sequence:

>759_bases
ATGAAACGAGCGCGCATCGCATATGCTGGTGGGATTTACTACGCAACTGAAGTAGATGGCAAGTTGCAATTAGATGATGG
ACGGATGCTAGTAGAAGAGGAAGTAACATGGCTCCCACCAGTTGAGCCGCGAACAGTATTTGCCCTCGGCCTAAATTACG
CAGATCACGCAAAAGAGTTATCATTTAACGCGCCAACAGAACCCCTTGTCTTCTTAAAGGGACCTAACACATTCATCGGT
CACCGTGGTCAAACATATCGTCCAGAAGATGTAACATATATGCACTATGAATGTGAGCTAGCTGTTGTTATGGGAAAAAC
AGCTCGAGGCGTTAAGAAAGAAGAGGCATACGATTACGTCGCTGGCTATACAGTAGCGAATGACTATGCAATACGGGATT
ATTTAGAAAATTATTATCGTCCAAATTTGAGAGTGAAAAACCGCGATACATGTACGCCAATCGGTCCATGGATTGTCGAT
GCATCCGACATTGACGACCCGATGAACTTAGCGCTCCGTACATATGTGAATGGCGAGCTGACGCAAGAAGGAACAACGAA
AGATATGATCTTTTCCATCCGGGACTTAATTGCCTACTTAAGTAGCTTTATGACATTAAGTAAAGGAGACATCATTTTAA
CAGGTACGCCAAAAGGATCCGTCGATACGAAAGTAGGAGACGAAGTCATCACCGAAATTGAAGGAATAGGCCGTCTGATC
AATACCATTGTTGGGGAAGAAACGTTTTCTAAACGATAG

Upstream 100 bases:

>100_bases
TCGTAAAAAATGGAGACCGCATTCGAATTGAAATCGAAGGAATTGGCAGCTTAGAAAATCAAGTCGTTTTAGAAAAAGAA
CTTGTGAGAGGAGGAGTACG

Downstream 100 bases:

>100_bases
AAATCGAGAGAGTAGCTAACAGAACGGCTACTCTTATTCTAGTAATCTATAAAAAGAAGGGGGCCTACGTCCATGCCGCA
TTTTATAGTCGAGTATACGG

Product: 4-hydroxyphenylacetate degradation bifunctional isomerase/decarboxylase,HpaG2 subunit

Products: NA

Alternate protein names: 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase; HHDD isomerase; 5-carboxymethyl-2-hydroxymuconate Delta-isomerase; 5-carboxymethyl-2-oxo-hex-3-ene-1,7-dioate decarboxylase; 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase; OPET decarboxylase [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MKRARIAYAGGIYYATEVDGKLQLDDGRMLVEEEVTWLPPVEPRTVFALGLNYADHAKELSFNAPTEPLVFLKGPNTFIG
HRGQTYRPEDVTYMHYECELAVVMGKTARGVKKEEAYDYVAGYTVANDYAIRDYLENYYRPNLRVKNRDTCTPIGPWIVD
ASDIDDPMNLALRTYVNGELTQEGTTKDMIFSIRDLIAYLSSFMTLSKGDIILTGTPKGSVDTKVGDEVITEIEGIGRLI
NTIVGEETFSKR

Sequences:

>Translated_252_residues
MKRARIAYAGGIYYATEVDGKLQLDDGRMLVEEEVTWLPPVEPRTVFALGLNYADHAKELSFNAPTEPLVFLKGPNTFIG
HRGQTYRPEDVTYMHYECELAVVMGKTARGVKKEEAYDYVAGYTVANDYAIRDYLENYYRPNLRVKNRDTCTPIGPWIVD
ASDIDDPMNLALRTYVNGELTQEGTTKDMIFSIRDLIAYLSSFMTLSKGDIILTGTPKGSVDTKVGDEVITEIEGIGRLI
NTIVGEETFSKR
>Mature_252_residues
MKRARIAYAGGIYYATEVDGKLQLDDGRMLVEEEVTWLPPVEPRTVFALGLNYADHAKELSFNAPTEPLVFLKGPNTFIG
HRGQTYRPEDVTYMHYECELAVVMGKTARGVKKEEAYDYVAGYTVANDYAIRDYLENYYRPNLRVKNRDTCTPIGPWIVD
ASDIDDPMNLALRTYVNGELTQEGTTKDMIFSIRDLIAYLSSFMTLSKGDIILTGTPKGSVDTKVGDEVITEIEGIGRLI
NTIVGEETFSKR

Specific function: Decarboxylates OPET (5-oxo-pent-3-ene-1,2,5- tricarboxylic acid) into HHDD (2-hydroxy-hept-2,4-diene-1,7- dioate) and isomerizes it to OHED (2-oxo-hept-3-ene-1,7-dioate) [H]

COG id: COG0179

COG function: function code Q; 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAH family [H]

Homologues:

Organism=Homo sapiens, GI156231349, Length=225, Percent_Identity=40.8888888888889, Blast_Score=165, Evalue=5e-41,
Organism=Homo sapiens, GI40786394, Length=225, Percent_Identity=40.8888888888889, Blast_Score=162, Evalue=2e-40,
Organism=Homo sapiens, GI215422413, Length=199, Percent_Identity=38.6934673366834, Blast_Score=134, Evalue=8e-32,
Organism=Homo sapiens, GI66348062, Length=196, Percent_Identity=39.2857142857143, Blast_Score=133, Evalue=1e-31,
Organism=Homo sapiens, GI13654274, Length=196, Percent_Identity=39.2857142857143, Blast_Score=133, Evalue=1e-31,
Organism=Escherichia coli, GI1787428, Length=193, Percent_Identity=39.3782383419689, Blast_Score=135, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17557057, Length=187, Percent_Identity=37.4331550802139, Blast_Score=123, Evalue=9e-29,
Organism=Saccharomyces cerevisiae, GI6324161, Length=224, Percent_Identity=29.9107142857143, Blast_Score=95, Evalue=1e-20,
Organism=Drosophila melanogaster, GI28572127, Length=227, Percent_Identity=41.4096916299559, Blast_Score=156, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24663695, Length=221, Percent_Identity=35.7466063348416, Blast_Score=135, Evalue=2e-32,
Organism=Drosophila melanogaster, GI28571789, Length=199, Percent_Identity=37.1859296482412, Blast_Score=126, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234
- InterPro:   IPR012684
- InterPro:   IPR012686 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: =5.3.3.10; =4.1.1.68 [H]

Molecular weight: Translated: 28319; Mature: 28319

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRARIAYAGGIYYATEVDGKLQLDDGRMLVEEEVTWLPPVEPRTVFALGLNYADHAKEL
CCCCEEEEECCEEEEEECCCEEEECCCCEEEECCCCCCCCCCCCEEEEEECCCHHHHHHC
SFNAPTEPLVFLKGPNTFIGHRGQTYRPEDVTYMHYECELAVVMGKTARGVKKEEAYDYV
CCCCCCCCEEEEECCCCEECCCCCCCCCCCCEEEEEEEEEEEEECCCCCCCCHHHHHHHH
AGYTVANDYAIRDYLENYYRPNLRVKNRDTCTPIGPWIVDASDIDDPMNLALRTYVNGEL
HCEEEECCHHHHHHHHHHCCCCCEECCCCCCCCCCCEEEECCCCCCHHHHEEEEEECCEE
TQEGTTKDMIFSIRDLIAYLSSFMTLSKGDIILTGTPKGSVDTKVGDEVITEIEGIGRLI
CCCCCHHHHHHHHHHHHHHHHHHHEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
NTIVGEETFSKR
HHHHCCHHHCCC
>Mature Secondary Structure
MKRARIAYAGGIYYATEVDGKLQLDDGRMLVEEEVTWLPPVEPRTVFALGLNYADHAKEL
CCCCEEEEECCEEEEEECCCEEEECCCCEEEECCCCCCCCCCCCEEEEEECCCHHHHHHC
SFNAPTEPLVFLKGPNTFIGHRGQTYRPEDVTYMHYECELAVVMGKTARGVKKEEAYDYV
CCCCCCCCEEEEECCCCEECCCCCCCCCCCCEEEEEEEEEEEEECCCCCCCCHHHHHHHH
AGYTVANDYAIRDYLENYYRPNLRVKNRDTCTPIGPWIVDASDIDDPMNLALRTYVNGEL
HCEEEECCHHHHHHHHHHCCCCCEECCCCCCCCCCCEEEECCCCCCHHHHEEEEEECCEE
TQEGTTKDMIFSIRDLIAYLSSFMTLSKGDIILTGTPKGSVDTKVGDEVITEIEGIGRLI
CCCCCHHHHHHHHHHHHHHHHHHHEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
NTIVGEETFSKR
HHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA