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The map label for this gene is gatB

Identifier: 152974140

GI number: 152974140

Start: 341953

End: 343383

Strand: Direct

Name: gatB

Synonym: Bcer98_0299

Alternate gene names: 152974140

Gene position: 341953-343383 (Clockwise)

Preceding gene: 152974139

Following gene: 152974141

Centisome position: 8.37

GC content: 36.55

Gene sequence:

>1431_bases
ATGAATTTTGAAACAATTATTGGTTTAGAGGTTCACGTTGAGTTAAAAACAAATTCGAAAATTTTCTCTGCGAGTCCAAC
AGAATTCGGAGCGGATCCAAATACACAAACAAGTGTAATTGATTTAGGATACCCAGGGGTACTTCCAACTTTAAACAAAG
AGGCAGTAAACTTTGCAATGAAAGCTGCAATGGCTTTAAACTGTGAAATCGCAACAGAAACGAAGTTTGACCGCAAGAAC
TATTTCTATCCAGATAATCCGAAAGCATACCAAATCTCTCAATTTGATAAACCAATTGGGCAAAATGGTTGGATTGAAAT
CGAAGTAGGTGGTCAAAAGAAACGAATCGGTATTACTCGTCTTCATTTAGAAGAAGATGCAGGTAAATCAACACATACAG
GTGAAGGTTACTCATTAGTAGACTACAATCGTCAAGGCCTTCCGTTAATTGAGATCGTATCTGAGCCAGATATGCGTACG
CCAGAAGAAGCATATGCATACTTAGAAAAATTAAAATCAATCATTCAATACACTGGTGTATCTGATTGTAAGATGGAAGA
AGGTTCTCTTCGCTGTGATGCAAACATTTCTCTTCGTCCAGTAGGGCAAGAGAAGTTTGGTACAAAAGCAGAACTAAAAA
ACTTAAACTCTTTCACTTATGTTCAAAAAGGTCTTGAGTTTGAACAAGCTCGTCAAGAAAAAGAATTACTATCCGGTGGT
ATTATTCAACAAGAAACACGTCGTTATGATGAAGCTACAAAGAAAACAATCTTAATGCGTGTAAAAGAAGGATCTGACGA
TTATCGTTACTTCCCAGAACCGGATTTAGTTGAACTTTATATTGATGAGGAATGGAAAGAACAAGTTCGTGCTTCCATTC
CTGAACTTCCAGATGCTCGTAAAGCACGTTATGTTGCAGAACTTGGTCTGCCAGCTTACGATGCACACGTATTAACATTA
ACAAAAGAAATGTCTGATTTCTTTGAGGAAACAGTTGCAGGCGGTGCAGATGCGAAGTTAGCATCAAACTGGCTAATGGG
TGAAGTGCTTGCATATTTAAACAAACAACAAAAAGAATTAAAAGATGTAGCTTTAACACCTGCTGGGCTATCTAAAATGA
TTCAATTAATCGAGAAAGGTACGATTTCTTCTAAAATTGCGAAGAAAGTATTTAACGAGTTAATTGAAAAAGGTGGAGAC
CCAGAAGAAATCGTGAAAGCAAAAGGTCTTGTTCAAATTTCTGATGAAGGTACACTTCGTAAGATTGTAACAGAAATCCT
TGATAATAATGCACAATCTATTGAAGACTTTAAAAACGGTAAAGACCGTGCAATTGGTTTCTTAGTTGGTCAAATTATGA
AAGCGACAAAAGGACAAGCAAACCCACCGCTTGTGAATAAAATCTTACTAGAAGAAATAAATAAGCGATAA

Upstream 100 bases:

>100_bases
AACATTTCGATGAAGCGACAATTTACCGCGTTGCACATGCATTTGAGCAAGCAACAGACTATCATACAAAAAAAGCAAGT
CTGTAAGGAGGCGAGCATAG

Downstream 100 bases:

>100_bases
AAAGAAAAGCATAGGCGGTTCGATTAGAAGAAGGAGGCGTTGGAGCCTTCGAAAGAGAAGTGTTTTTTGCTTCACTACAG
GAGGAGAAACGCCTGATCCT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MNFETIIGLEVHVELKTNSKIFSASPTEFGADPNTQTSVIDLGYPGVLPTLNKEAVNFAMKAAMALNCEIATETKFDRKN
YFYPDNPKAYQISQFDKPIGQNGWIEIEVGGQKKRIGITRLHLEEDAGKSTHTGEGYSLVDYNRQGLPLIEIVSEPDMRT
PEEAYAYLEKLKSIIQYTGVSDCKMEEGSLRCDANISLRPVGQEKFGTKAELKNLNSFTYVQKGLEFEQARQEKELLSGG
IIQQETRRYDEATKKTILMRVKEGSDDYRYFPEPDLVELYIDEEWKEQVRASIPELPDARKARYVAELGLPAYDAHVLTL
TKEMSDFFEETVAGGADAKLASNWLMGEVLAYLNKQQKELKDVALTPAGLSKMIQLIEKGTISSKIAKKVFNELIEKGGD
PEEIVKAKGLVQISDEGTLRKIVTEILDNNAQSIEDFKNGKDRAIGFLVGQIMKATKGQANPPLVNKILLEEINKR

Sequences:

>Translated_476_residues
MNFETIIGLEVHVELKTNSKIFSASPTEFGADPNTQTSVIDLGYPGVLPTLNKEAVNFAMKAAMALNCEIATETKFDRKN
YFYPDNPKAYQISQFDKPIGQNGWIEIEVGGQKKRIGITRLHLEEDAGKSTHTGEGYSLVDYNRQGLPLIEIVSEPDMRT
PEEAYAYLEKLKSIIQYTGVSDCKMEEGSLRCDANISLRPVGQEKFGTKAELKNLNSFTYVQKGLEFEQARQEKELLSGG
IIQQETRRYDEATKKTILMRVKEGSDDYRYFPEPDLVELYIDEEWKEQVRASIPELPDARKARYVAELGLPAYDAHVLTL
TKEMSDFFEETVAGGADAKLASNWLMGEVLAYLNKQQKELKDVALTPAGLSKMIQLIEKGTISSKIAKKVFNELIEKGGD
PEEIVKAKGLVQISDEGTLRKIVTEILDNNAQSIEDFKNGKDRAIGFLVGQIMKATKGQANPPLVNKILLEEINKR
>Mature_476_residues
MNFETIIGLEVHVELKTNSKIFSASPTEFGADPNTQTSVIDLGYPGVLPTLNKEAVNFAMKAAMALNCEIATETKFDRKN
YFYPDNPKAYQISQFDKPIGQNGWIEIEVGGQKKRIGITRLHLEEDAGKSTHTGEGYSLVDYNRQGLPLIEIVSEPDMRT
PEEAYAYLEKLKSIIQYTGVSDCKMEEGSLRCDANISLRPVGQEKFGTKAELKNLNSFTYVQKGLEFEQARQEKELLSGG
IIQQETRRYDEATKKTILMRVKEGSDDYRYFPEPDLVELYIDEEWKEQVRASIPELPDARKARYVAELGLPAYDAHVLTL
TKEMSDFFEETVAGGADAKLASNWLMGEVLAYLNKQQKELKDVALTPAGLSKMIQLIEKGTISSKIAKKVFNELIEKGGD
PEEIVKAKGLVQISDEGTLRKIVTEILDNNAQSIEDFKNGKDRAIGFLVGQIMKATKGQANPPLVNKILLEEINKR

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=492, Percent_Identity=36.9918699186992, Blast_Score=313, Evalue=2e-85,
Organism=Caenorhabditis elegans, GI115534094, Length=299, Percent_Identity=39.4648829431438, Blast_Score=219, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6319391, Length=515, Percent_Identity=29.7087378640777, Blast_Score=220, Evalue=5e-58,
Organism=Drosophila melanogaster, GI45550808, Length=487, Percent_Identity=32.6488706365503, Blast_Score=268, Evalue=8e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_BACCN (A7GKK4)

Other databases:

- EMBL:   CP000764
- RefSeq:   YP_001373657.1
- ProteinModelPortal:   A7GKK4
- SMR:   A7GKK4
- STRING:   A7GKK4
- EnsemblBacteria:   EBBACT00000039542
- GeneID:   5344748
- GenomeReviews:   CP000764_GR
- KEGG:   bcy:Bcer98_0299
- eggNOG:   COG0064
- GeneTree:   EBGT00050000002603
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- ProtClustDB:   PRK05477
- BioCyc:   BCER315749:BCER98_0299-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 53337; Mature: 53337

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFETIIGLEVHVELKTNSKIFSASPTEFGADPNTQTSVIDLGYPGVLPTLNKEAVNFAM
CCCCEEEEEEEEEEEECCCEEEECCCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHH
KAAMALNCEIATETKFDRKNYFYPDNPKAYQISQFDKPIGQNGWIEIEVGGQKKRIGITR
HHHHHEEEEEECCCCCCCCCCCCCCCCCEEEHHHHCCCCCCCCEEEEEECCCEEEECEEE
LHLEEDAGKSTHTGEGYSLVDYNRQGLPLIEIVSEPDMRTPEEAYAYLEKLKSIIQYTGV
EEEECCCCCCCCCCCCEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCC
SDCKMEEGSLRCDANISLRPVGQEKFGTKAELKNLNSFTYVQKGLEFEQARQEKELLSGG
CCCEECCCCEEECCCCEEEECCHHHCCCHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHCC
IIQQETRRYDEATKKTILMRVKEGSDDYRYFPEPDLVELYIDEEWKEQVRASIPELPDAR
CHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCEEEEEECHHHHHHHHHCCCCCCCHH
KARYVAELGLPAYDAHVLTLTKEMSDFFEETVAGGADAKLASNWLMGEVLAYLNKQQKEL
HHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KDVALTPAGLSKMIQLIEKGTISSKIAKKVFNELIEKGGDPEEIVKAKGLVQISDEGTLR
HHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEECCCCHHH
KIVTEILDNNAQSIEDFKNGKDRAIGFLVGQIMKATKGQANPPLVNKILLEEINKR
HHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MNFETIIGLEVHVELKTNSKIFSASPTEFGADPNTQTSVIDLGYPGVLPTLNKEAVNFAM
CCCCEEEEEEEEEEEECCCEEEECCCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHH
KAAMALNCEIATETKFDRKNYFYPDNPKAYQISQFDKPIGQNGWIEIEVGGQKKRIGITR
HHHHHEEEEEECCCCCCCCCCCCCCCCCEEEHHHHCCCCCCCCEEEEEECCCEEEECEEE
LHLEEDAGKSTHTGEGYSLVDYNRQGLPLIEIVSEPDMRTPEEAYAYLEKLKSIIQYTGV
EEEECCCCCCCCCCCCEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCC
SDCKMEEGSLRCDANISLRPVGQEKFGTKAELKNLNSFTYVQKGLEFEQARQEKELLSGG
CCCEECCCCEEECCCCEEEECCHHHCCCHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHCC
IIQQETRRYDEATKKTILMRVKEGSDDYRYFPEPDLVELYIDEEWKEQVRASIPELPDAR
CHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCEEEEEECHHHHHHHHHCCCCCCCHH
KARYVAELGLPAYDAHVLTLTKEMSDFFEETVAGGADAKLASNWLMGEVLAYLNKQQKEL
HHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KDVALTPAGLSKMIQLIEKGTISSKIAKKVFNELIEKGGDPEEIVKAKGLVQISDEGTLR
HHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEECCCCHHH
KIVTEILDNNAQSIEDFKNGKDRAIGFLVGQIMKATKGQANPPLVNKILLEEINKR
HHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA