Click here to switch to the map view.

The map label for this gene is dagK [H]

Identifier: 152974141

GI number: 152974141

Start: 343887

End: 344792

Strand: Direct

Name: dagK [H]

Synonym: Bcer98_0300

Alternate gene names: 152974141

Gene position: 343887-344792 (Clockwise)

Preceding gene: 152974140

Following gene: 152974143

Centisome position: 8.41

GC content: 38.52

Gene sequence:

>906_bases
ATGATGAAACGAGCAAGAATAATTTATAATCCTACTTCTGGTCGTGAGTTATTTAAAAAGCATTTACCAGAAGTATTACA
GAAATTAGAACAAGCTGGATATGAAACATCTTGTCACGCGACAACAGGGCCTGGAGACGCAACAATTGCGGCAAGGCAAG
CGGCAAAGCGTAAATTTGATGTAGTCATTGCAGCAGGCGGTGATGGGACTTTAAATGAAGTGGTAAATGGTTTAGTAGAG
CAAGAACATCGTCCGAAGTTTGGAATTATTCCAGTTGGAACAACGAATGATTTTGCACGTGCTATTGGTGTCCCACGTTC
TATTGAAGAAGCGGCAGATATTATCTGTGAAGGGAAAACGGTCCCGTTAGATCTTGGTAGAGCAAACGATACATATTTTA
TTAATATTGCTGGAGGCGGACGTATTACAGAGTTAACGTATGAAGTGCCGAGCAAATTAAAAACAGTGCTAGGACAATTA
GCATATTACTTAAAAGGAATTGAAATGTTACCGTCATTACATCCAACGTATGTTGAGATTGAGTATGATGGAAAGTTGCT
GCAAGAAGAAATTACGATGTTTTTAATTACAAATACACGTTCAGTAGGTGGATTTGAAAAGGTAGCACCATATGCTTCTA
TTAATGATGGGTTATTTGACTTGTTAGTACTGAAAAAAGGATCAATAGCGGATTTAATTAAAGCGGCAACTCAAGCGCAG
CGCGGTGAGCATATTAACAATCCAAAAGTATTATATACGCAAGCAAATCGTATTAAGGTGCATTCGCCTGATAAGTTAAT
GATCAACTTAGATGGTGAATACGGTGGAGATGCACCGATGGAGTTTGAAAACATCTATCATTGCTTAGAACTATTTGTTC
CTGAGCAGAAAGAGGACGCCTTATAA

Upstream 100 bases:

>100_bases
GGATATGGAGATGAGAGGAGGCAGGCATAGGAAGGCTGGAGAAAAGTTGGATATATTCTTTTTTCTGAATTGATGAATGA
GAAAATTCCTGTGCTAATAC

Downstream 100 bases:

>100_bases
TGGCATCCTCTTTTTTAATAGCTTATTATTGCTTTGTATCATACATATAGGCGCGTGTCCATGGAATATCATCATTAATC
CCTTTAGTAAATACGAATTG

Product: putative lipid kinase

Products: NA

Alternate protein names: DAG kinase; DAGK [H]

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MMKRARIIYNPTSGRELFKKHLPEVLQKLEQAGYETSCHATTGPGDATIAARQAAKRKFDVVIAAGGDGTLNEVVNGLVE
QEHRPKFGIIPVGTTNDFARAIGVPRSIEEAADIICEGKTVPLDLGRANDTYFINIAGGGRITELTYEVPSKLKTVLGQL
AYYLKGIEMLPSLHPTYVEIEYDGKLLQEEITMFLITNTRSVGGFEKVAPYASINDGLFDLLVLKKGSIADLIKAATQAQ
RGEHINNPKVLYTQANRIKVHSPDKLMINLDGEYGGDAPMEFENIYHCLELFVPEQKEDAL

Sequences:

>Translated_301_residues
MMKRARIIYNPTSGRELFKKHLPEVLQKLEQAGYETSCHATTGPGDATIAARQAAKRKFDVVIAAGGDGTLNEVVNGLVE
QEHRPKFGIIPVGTTNDFARAIGVPRSIEEAADIICEGKTVPLDLGRANDTYFINIAGGGRITELTYEVPSKLKTVLGQL
AYYLKGIEMLPSLHPTYVEIEYDGKLLQEEITMFLITNTRSVGGFEKVAPYASINDGLFDLLVLKKGSIADLIKAATQAQ
RGEHINNPKVLYTQANRIKVHSPDKLMINLDGEYGGDAPMEFENIYHCLELFVPEQKEDAL
>Mature_301_residues
MMKRARIIYNPTSGRELFKKHLPEVLQKLEQAGYETSCHATTGPGDATIAARQAAKRKFDVVIAAGGDGTLNEVVNGLVE
QEHRPKFGIIPVGTTNDFARAIGVPRSIEEAADIICEGKTVPLDLGRANDTYFINIAGGGRITELTYEVPSKLKTVLGQL
AYYLKGIEMLPSLHPTYVEIEYDGKLLQEEITMFLITNTRSVGGFEKVAPYASINDGLFDLLVLKKGSIADLIKAATQAQ
RGEHINNPKVLYTQANRIKVHSPDKLMINLDGEYGGDAPMEFENIYHCLELFVPEQKEDAL

Specific function: Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is a key enzyme involved in the production of lipoteichoic acid by reintroducing DAG formed from the breakdown of membrane phospholipids into the phosphatidylglycerol biosynthet

COG id: COG1597

COG function: function code IR; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DAGKc domain [H]

Homologues:

Organism=Escherichia coli, GI1788402, Length=277, Percent_Identity=31.4079422382672, Blast_Score=145, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017438
- InterPro:   IPR005218
- InterPro:   IPR001206 [H]

Pfam domain/function: PF00781 DAGK_cat [H]

EC number: =2.7.1.107 [H]

Molecular weight: Translated: 33149; Mature: 33149

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKRARIIYNPTSGRELFKKHLPEVLQKLEQAGYETSCHATTGPGDATIAARQAAKRKFD
CCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCEEE
VVIAAGGDGTLNEVVNGLVEQEHRPKFGIIPVGTTNDFARAIGVPRSIEEAADIICEGKT
EEEEECCCCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHCCCCHHHHHHHHEECCCC
VPLDLGRANDTYFINIAGGGRITELTYEVPSKLKTVLGQLAYYLKGIEMLPSLHPTYVEI
CEEECCCCCCEEEEEEECCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
EYDGKLLQEEITMFLITNTRSVGGFEKVAPYASINDGLFDLLVLKKGSIADLIKAATQAQ
EECCHHHHCCEEEEEEECCCCCCCHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHH
RGEHINNPKVLYTQANRIKVHSPDKLMINLDGEYGGDAPMEFENIYHCLELFVPEQKEDA
CCCCCCCCEEEEEECCEEEEECCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCC
L
C
>Mature Secondary Structure
MMKRARIIYNPTSGRELFKKHLPEVLQKLEQAGYETSCHATTGPGDATIAARQAAKRKFD
CCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCEEE
VVIAAGGDGTLNEVVNGLVEQEHRPKFGIIPVGTTNDFARAIGVPRSIEEAADIICEGKT
EEEEECCCCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHCCCCHHHHHHHHEECCCC
VPLDLGRANDTYFINIAGGGRITELTYEVPSKLKTVLGQLAYYLKGIEMLPSLHPTYVEI
CEEECCCCCCEEEEEEECCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
EYDGKLLQEEITMFLITNTRSVGGFEKVAPYASINDGLFDLLVLKKGSIADLIKAATQAQ
EECCHHHHCCEEEEEEECCCCCCCHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHH
RGEHINNPKVLYTQANRIKVHSPDKLMINLDGEYGGDAPMEFENIYHCLELFVPEQKEDA
CCCCCCCCEEEEEECCEEEEECCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCC
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]