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The map label for this gene is gatA [H]

Identifier: 152974139

GI number: 152974139

Start: 340481

End: 341938

Strand: Direct

Name: gatA [H]

Synonym: Bcer98_0298

Alternate gene names: 152974139

Gene position: 340481-341938 (Clockwise)

Preceding gene: 152974138

Following gene: 152974140

Centisome position: 8.33

GC content: 38.55

Gene sequence:

>1458_bases
ATGTCATTATTTGATCATTCTGTATCAGAGTTACATAAGAAATTAAACAACAAAGAAATTTCCGTTACGGATTTAGTAGA
AGAGTCTTACAAACGTATTGCGGATGTTGAAGATAACGTAAAAGCGTTTCTTACATTAAATGAAGAAAACGCACGTGCGA
AAGCGAAAGAATTAGATGCAAAAATTGGTGCTGAAGATAATGGTCTATTATTCGGTATGCCAATCGGTGTTAAAGATAAC
ATTGTAACAAAAGGTCTTCGTACAACTTGTGCAAGCAAAATGTTAGCAAACTTTGATCCAATTTATGATGCGACAGTTGT
GCAGAAGTTAAACGAAGCTGACACAATTACAATCGGTAAATTAAATATGGACGAGTTTGCAATGGGTTCTTCCAATGAAA
ACTCTGGTTTTTATGCTACGAAAAATCCATGGAATTTAGAATATGTTCCAGGTGGCTCTAGCGGTGGTTCTGCAGCAGCA
GTTGCAGCAGGAGAAGTATTATTCTCTCTAGGTTCTGATACCGGCGGTTCCATCCGTCAGCCAGCTGCATATTGCGGTGT
TGTAGGTTTAAAACCAACTTATGGACGTGTATCTCGTTATGGTTTAGTCGCATTTGCATCTTCACTTGATCAAATCGGAC
CAATTACACGTACTGTAGAAGACAATGCGTACTTATTACAAGCTATTTCAGGTGTTGACCGTATGGATGCAACTTCTGCA
AATATTGAAGTAGGTAACTATTTAGCTGGTTTAACAGGCGATGTAAAAGGTTTACGTATTGCTGTACCAAAAGAATATTT
AGGAGAAGGTGTTGGTGAAGAAGCTCGTGAGTCCGTACTAGCTGCTTTAAAAGTGTTAGAAGGTATGGGCGCAACTTGGG
AAGAAGTATCCCTTCCGCACTCTAAATACGCGCTAGCAACGTACTACTTAATTTCTTCTTCTGAAGCGTCTTCTAATCTG
TCACGCTTTGATGGCGTACGCTACGGTGTTCGTTCTGATAATGTGAATAACTTAATGGATCTTTACAAAAATACGCGTAG
TGAAGGGTTCGGAGACGAAGTAAAACGTCGTATTATGCTCGGTACATTTGCACTTAGCTCTGGTTACTATGATGCATATT
ACAAAAAAGCACAACAAGTACGTACATTAATTAAAAATGACTTTGAAAATGTATTTGCAAATTACGATGTGATTATTGGA
CCAACAACGCCAACTCCAGCGTTTAAAGTAGGCGAAAAAATTGATGATCCAATGACAATGTATGCAAATGACATTTTAAC
AATCCCAGTAAACTTAGCAGGTGTTCCAGCGATTTCTGTTCCATGTGGATTCGGCGCTAATAATATGCCGCTTGGTTTGC
AAATCATTGGTAAACATTTCGATGAAGCGACAATTTACCGCGTTGCACATGCATTTGAGCAAGCAACAGACTATCATACA
AAAAAAGCAAGTCTGTAA

Upstream 100 bases:

>100_bases
GAACAAGGTTTACCAGTAGAAGAAGTATTAAAAAATGCACCGGATCACAAAGAGAATCAAATCCGTGTTCCAGCAGTATT
AGAATAGGGGGGGAATTTCG

Downstream 100 bases:

>100_bases
GGAGGCGAGCATAGATGAATTTTGAAACAATTATTGGTTTAGAGGTTCACGTTGAGTTAAAAACAAATTCGAAAATTTTC
TCTGCGAGTCCAACAGAATT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: ADP; phosphate; Gln-tRNA(Gln); L-glutamate

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MSLFDHSVSELHKKLNNKEISVTDLVEESYKRIADVEDNVKAFLTLNEENARAKAKELDAKIGAEDNGLLFGMPIGVKDN
IVTKGLRTTCASKMLANFDPIYDATVVQKLNEADTITIGKLNMDEFAMGSSNENSGFYATKNPWNLEYVPGGSSGGSAAA
VAAGEVLFSLGSDTGGSIRQPAAYCGVVGLKPTYGRVSRYGLVAFASSLDQIGPITRTVEDNAYLLQAISGVDRMDATSA
NIEVGNYLAGLTGDVKGLRIAVPKEYLGEGVGEEARESVLAALKVLEGMGATWEEVSLPHSKYALATYYLISSSEASSNL
SRFDGVRYGVRSDNVNNLMDLYKNTRSEGFGDEVKRRIMLGTFALSSGYYDAYYKKAQQVRTLIKNDFENVFANYDVIIG
PTTPTPAFKVGEKIDDPMTMYANDILTIPVNLAGVPAISVPCGFGANNMPLGLQIIGKHFDEATIYRVAHAFEQATDYHT
KKASL

Sequences:

>Translated_485_residues
MSLFDHSVSELHKKLNNKEISVTDLVEESYKRIADVEDNVKAFLTLNEENARAKAKELDAKIGAEDNGLLFGMPIGVKDN
IVTKGLRTTCASKMLANFDPIYDATVVQKLNEADTITIGKLNMDEFAMGSSNENSGFYATKNPWNLEYVPGGSSGGSAAA
VAAGEVLFSLGSDTGGSIRQPAAYCGVVGLKPTYGRVSRYGLVAFASSLDQIGPITRTVEDNAYLLQAISGVDRMDATSA
NIEVGNYLAGLTGDVKGLRIAVPKEYLGEGVGEEARESVLAALKVLEGMGATWEEVSLPHSKYALATYYLISSSEASSNL
SRFDGVRYGVRSDNVNNLMDLYKNTRSEGFGDEVKRRIMLGTFALSSGYYDAYYKKAQQVRTLIKNDFENVFANYDVIIG
PTTPTPAFKVGEKIDDPMTMYANDILTIPVNLAGVPAISVPCGFGANNMPLGLQIIGKHFDEATIYRVAHAFEQATDYHT
KKASL
>Mature_484_residues
SLFDHSVSELHKKLNNKEISVTDLVEESYKRIADVEDNVKAFLTLNEENARAKAKELDAKIGAEDNGLLFGMPIGVKDNI
VTKGLRTTCASKMLANFDPIYDATVVQKLNEADTITIGKLNMDEFAMGSSNENSGFYATKNPWNLEYVPGGSSGGSAAAV
AAGEVLFSLGSDTGGSIRQPAAYCGVVGLKPTYGRVSRYGLVAFASSLDQIGPITRTVEDNAYLLQAISGVDRMDATSAN
IEVGNYLAGLTGDVKGLRIAVPKEYLGEGVGEEARESVLAALKVLEGMGATWEEVSLPHSKYALATYYLISSSEASSNLS
RFDGVRYGVRSDNVNNLMDLYKNTRSEGFGDEVKRRIMLGTFALSSGYYDAYYKKAQQVRTLIKNDFENVFANYDVIIGP
TTPTPAFKVGEKIDDPMTMYANDILTIPVNLAGVPAISVPCGFGANNMPLGLQIIGKHFDEATIYRVAHAFEQATDYHTK
KASL

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=501, Percent_Identity=37.9241516966068, Blast_Score=322, Evalue=7e-88,
Organism=Homo sapiens, GI195972892, Length=487, Percent_Identity=25.4620123203285, Blast_Score=135, Evalue=9e-32,
Organism=Homo sapiens, GI166795287, Length=161, Percent_Identity=34.1614906832298, Blast_Score=71, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17543272, Length=439, Percent_Identity=37.3576309794989, Blast_Score=270, Evalue=9e-73,
Organism=Caenorhabditis elegans, GI17537465, Length=501, Percent_Identity=25.5489021956088, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17556264, Length=187, Percent_Identity=34.7593582887701, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17538252, Length=515, Percent_Identity=22.3300970873786, Blast_Score=82, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI71990152, Length=156, Percent_Identity=33.3333333333333, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17556278, Length=170, Percent_Identity=35.2941176470588, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17556276, Length=170, Percent_Identity=35.2941176470588, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17538254, Length=227, Percent_Identity=29.0748898678414, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=462, Percent_Identity=34.8484848484849, Blast_Score=244, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6319685, Length=413, Percent_Identity=30.9927360774818, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6320448, Length=465, Percent_Identity=24.7311827956989, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24648113, Length=502, Percent_Identity=37.0517928286853, Blast_Score=294, Evalue=9e-80,
Organism=Drosophila melanogaster, GI24652985, Length=498, Percent_Identity=26.5060240963855, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI19922090, Length=498, Percent_Identity=26.5060240963855, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24652981, Length=498, Percent_Identity=26.5060240963855, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24652983, Length=498, Percent_Identity=26.5060240963855, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI161078093, Length=509, Percent_Identity=27.3084479371316, Blast_Score=122, Evalue=7e-28,
Organism=Drosophila melanogaster, GI24644968, Length=503, Percent_Identity=26.6401590457256, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI45550774, Length=492, Percent_Identity=24.5934959349593, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24648435, Length=492, Percent_Identity=24.390243902439, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24648437, Length=492, Percent_Identity=24.390243902439, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI21356731, Length=499, Percent_Identity=24.0480961923848, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24648441, Length=417, Percent_Identity=25.1798561151079, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24648439, Length=417, Percent_Identity=25.1798561151079, Blast_Score=98, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.7

Molecular weight: Translated: 52441; Mature: 52310

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLFDHSVSELHKKLNNKEISVTDLVEESYKRIADVEDNVKAFLTLNEENARAKAKELDA
CCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHH
KIGAEDNGLLFGMPIGVKDNIVTKGLRTTCASKMLANFDPIYDATVVQKLNEADTITIGK
HCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEE
LNMDEFAMGSSNENSGFYATKNPWNLEYVPGGSSGGSAAAVAAGEVLFSLGSDTGGSIRQ
CCHHHHCCCCCCCCCCEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCC
PAAYCGVVGLKPTYGRVSRYGLVAFASSLDQIGPITRTVEDNAYLLQAISGVDRMDATSA
CHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHCCCCCCCCCC
NIEVGNYLAGLTGDVKGLRIAVPKEYLGEGVGEEARESVLAALKVLEGMGATWEEVSLPH
CEEECCCHHCCCCCCCCEEEECCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCC
SKYALATYYLISSSEASSNLSRFDGVRYGVRSDNVNNLMDLYKNTRSEGFGDEVKRRIML
CHHEEEEEEEEECCCCHHHHHHHCCCEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHH
GTFALSSGYYDAYYKKAQQVRTLIKNDFENVFANYDVIIGPTTPTPAFKVGEKIDDPMTM
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHCCCCCCCHHH
YANDILTIPVNLAGVPAISVPCGFGANNMPLGLQIIGKHFDEATIYRVAHAFEQATDYHT
HCCCEEEEEEECCCCCEEECCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCH
KKASL
HCCCC
>Mature Secondary Structure 
SLFDHSVSELHKKLNNKEISVTDLVEESYKRIADVEDNVKAFLTLNEENARAKAKELDA
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHH
KIGAEDNGLLFGMPIGVKDNIVTKGLRTTCASKMLANFDPIYDATVVQKLNEADTITIGK
HCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEE
LNMDEFAMGSSNENSGFYATKNPWNLEYVPGGSSGGSAAAVAAGEVLFSLGSDTGGSIRQ
CCHHHHCCCCCCCCCCEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCC
PAAYCGVVGLKPTYGRVSRYGLVAFASSLDQIGPITRTVEDNAYLLQAISGVDRMDATSA
CHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHCCCCCCCCCC
NIEVGNYLAGLTGDVKGLRIAVPKEYLGEGVGEEARESVLAALKVLEGMGATWEEVSLPH
CEEECCCHHCCCCCCCCEEEECCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCC
SKYALATYYLISSSEASSNLSRFDGVRYGVRSDNVNNLMDLYKNTRSEGFGDEVKRRIML
CHHEEEEEEEEECCCCHHHHHHHCCCEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHH
GTFALSSGYYDAYYKKAQQVRTLIKNDFENVFANYDVIIGPTTPTPAFKVGEKIDDPMTM
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHCCCCCCCHHH
YANDILTIPVNLAGVPAISVPCGFGANNMPLGLQIIGKHFDEATIYRVAHAFEQATDYHT
HCCCEEEEEEECCCCCEEECCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCH
KKASL
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; Glu-tRNA(Gln); L-glutamine

Specific reaction: ATP + Glu-tRNA(Gln) + L-glutamine = ADP + phosphate + Gln-tRNA(Gln) + L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA