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The map label for this gene is pntA [H]

Identifier: 152970078

GI number: 152970078

Start: 1696001

End: 1697545

Strand: Direct

Name: pntA [H]

Synonym: KPN_01526

Alternate gene names: 152970078

Gene position: 1696001-1697545 (Clockwise)

Preceding gene: 152970074

Following gene: 152970079

Centisome position: 31.91

GC content: 58.32

Gene sequence:

>1545_bases
ATGGAAGGGAAAACTATGCGTATTGGTGTACCACAAGAAAGGCTAGCCCAGGAGACCCGGGCCGCCGCCACGCCGAAGAC
CGTTGAGCAATTGCTCAAACTCGGCTTTAGCGTCGCGGTAGAAAGCGGCGCCGGGAAGCTGGCCAGCTTTGACGATGAGG
CCTTTGCCGAGGCAGGAGCTGAAATCGTGACCGGCGACGAGGTATGGCAGTCTGATGTCATCCTTAAGGTCAATGCTCCG
AATGACGATGAAATTGCCCTGCTGAATCCGGGGACCACGCTGATTAGTTTTATCTGGCCGGCGCAAAACCCGCAGCTGAT
GGAAAAACTGGCGGCCCGTAATATCAACGTAATGGCGATGGATTCCGTGCCGCGTATTTCCCGCGCCCAGTCGCTGGATG
CGCTCAGCTCAATGGCCAACATCGCCGGGTACCGCGCTATCGTTGAAGCGGCGCATGAATTTGGTCGATTCTTTACCGGG
CAGATTACGGCGGCAGGCAAGGTTCCGCCCGCCAAGGTGATGGTCATTGGCGCCGGGGTAGCCGGTCTGGCGGCGATTGG
CGCCGCCAACAGCCTGGGCGCTATTGTTCGCGCCTTCGATACCCGTCCGGAAGTGAAGGAGCAGGTGCAGAGTATGGGCG
CCGAGTTCCTCGAGCTGGATTTTAAAGAGGAAGCGGGCAGCGGCGATGGCTACGCCAAGGTGATGTCTGAGGCCTTTATT
AAGGCTGAAATGGCGCTGTTCGCCGCGCAGGCGAAAGAGGTCGATATCATTGTCACCACGGCGTTAATCCCGGGCAAACC
GGCGCCGAAGCTGATCACCCGCGAAATGGTCGACTCCATGAAATCGGGCAGCGTGGTGGTCGATCTGGCCTCGCAAAACG
GCGGCAACTGTGAGTACACCGTACCGGGCGAAGTGGTGACCACCGCCAACGGCGTGAAAATCATCGGTTATACCGATCTT
CCTGGCCGTCTGCCGACCCAGTCCTCCCAGCTCTACGGCACCAACCTGGTGAACCTGCTGAAGCTGCTGTGCAAAGAGAA
AGACGGCAATATCGTTATCGATTTCGATGACGTGGTAGTGCGCGGTGTGACCGTTGTTCGCGAAGGTGAAATCACCTGGC
CGGCGCCGCCGATTCAGGTCTCCGCTCAGCCGCAGGCCGCGGCGAAAAAAGTGGAAGCGCCGAAAGAAGCGGTGAAACCG
GCGTCGCCGTGGCGCAAATATGCGCTGATGGCGCTGGCGATTATTCTCTTCGGCTGGCTGGCCAACGTGGCGCCAAAAGA
GTTCCTCGGCCATTTCACCGTCTTCGCGCTGGCCTGCGTGGTGGGTTACTACGTGGTGTGGAACGTCTCCCATGCGCTGC
ATACGCCGCTGATGTCGGTGACCAATGCCATTTCGGGCATTATCGTGGTTGGCGCGTTGTTGCAGATAGGCCACGGCGGC
TGGGTCAGCTTCCTGAGCTTTATCGCGGTGCTGATCGCCAGCATTAATATTTTCGGTGGTTTTACCGTGACTCAGCGCAT
GCTGAAAATGTTTCGCAAAGGATAA

Upstream 100 bases:

>100_bases
AGTTTGTTACTGTTTCATTTTGTAGACAGCGAAAGATGGCGAGGAAGATTTTGCCGTCCTGGCGTTGTTGGGCAGAAAAG
TAAACCATCATCAAAAACCG

Downstream 100 bases:

>100_bases
GGGGTAGCACATGTCTGGTGGATTAGTAACGGCTGCATACATTGTTGCTGCAATCCTGTTTATTTTCAGCCTGGCGGGCC
TGTCGAAACACGAAACGTCG

Product: NAD(P) transhydrogenase subunit alpha

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit alpha; Pyridine nucleotide transhydrogenase subunit alpha [H]

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MEGKTMRIGVPQERLAQETRAAATPKTVEQLLKLGFSVAVESGAGKLASFDDEAFAEAGAEIVTGDEVWQSDVILKVNAP
NDDEIALLNPGTTLISFIWPAQNPQLMEKLAARNINVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTG
QITAAGKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKEEAGSGDGYAKVMSEAFI
KAEMALFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMKSGSVVVDLASQNGGNCEYTVPGEVVTTANGVKIIGYTDL
PGRLPTQSSQLYGTNLVNLLKLLCKEKDGNIVIDFDDVVVRGVTVVREGEITWPAPPIQVSAQPQAAAKKVEAPKEAVKP
ASPWRKYALMALAIILFGWLANVAPKEFLGHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGHGG
WVSFLSFIAVLIASINIFGGFTVTQRMLKMFRKG

Sequences:

>Translated_514_residues
MEGKTMRIGVPQERLAQETRAAATPKTVEQLLKLGFSVAVESGAGKLASFDDEAFAEAGAEIVTGDEVWQSDVILKVNAP
NDDEIALLNPGTTLISFIWPAQNPQLMEKLAARNINVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTG
QITAAGKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKEEAGSGDGYAKVMSEAFI
KAEMALFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMKSGSVVVDLASQNGGNCEYTVPGEVVTTANGVKIIGYTDL
PGRLPTQSSQLYGTNLVNLLKLLCKEKDGNIVIDFDDVVVRGVTVVREGEITWPAPPIQVSAQPQAAAKKVEAPKEAVKP
ASPWRKYALMALAIILFGWLANVAPKEFLGHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGHGG
WVSFLSFIAVLIASINIFGGFTVTQRMLKMFRKG
>Mature_514_residues
MEGKTMRIGVPQERLAQETRAAATPKTVEQLLKLGFSVAVESGAGKLASFDDEAFAEAGAEIVTGDEVWQSDVILKVNAP
NDDEIALLNPGTTLISFIWPAQNPQLMEKLAARNINVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTG
QITAAGKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKEEAGSGDGYAKVMSEAFI
KAEMALFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMKSGSVVVDLASQNGGNCEYTVPGEVVTTANGVKIIGYTDL
PGRLPTQSSQLYGTNLVNLLKLLCKEKDGNIVIDFDDVVVRGVTVVREGEITWPAPPIQVSAQPQAAAKKVEAPKEAVKP
ASPWRKYALMALAIILFGWLANVAPKEFLGHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGHGG
WVSFLSFIAVLIASINIFGGFTVTQRMLKMFRKG

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG3288

COG function: function code C; NAD/NADP transhydrogenase alpha subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=534, Percent_Identity=51.310861423221, Blast_Score=484, Evalue=1e-137,
Organism=Homo sapiens, GI122939153, Length=534, Percent_Identity=51.310861423221, Blast_Score=484, Evalue=1e-137,
Organism=Escherichia coli, GI1787887, Length=509, Percent_Identity=88.8015717092338, Blast_Score=926, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17550456, Length=526, Percent_Identity=51.3307984790874, Blast_Score=489, Evalue=1e-138,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR004571 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 54687; Mature: 54687

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGKTMRIGVPQERLAQETRAAATPKTVEQLLKLGFSVAVESGAGKLASFDDEAFAEAGA
CCCCEEEECCCHHHHHHHHHHCCCHHHHHHHHHCCHHEEEECCCCCCCCCCHHHHHHCCC
EIVTGDEVWQSDVILKVNAPNDDEIALLNPGTTLISFIWPAQNPQLMEKLAARNINVMAM
EEEECCHHCCCCEEEEEECCCCCCEEEECCCCEEEEEECCCCCHHHHHHHHHCCCCEEEE
DSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAAGKVPPAKVMVIGAGV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECEEEECCCCCCCEEEEEECCH
AGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKEEAGSGDGYAKVMSEAFI
HHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHCCCHHHEEECHHCCCCCCHHHHHHHHHHH
KAEMALFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMKSGSVVVDLASQNGGNCEYT
HHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEE
VPGEVVTTANGVKIIGYTDLPGRLPTQSSQLYGTNLVNLLKLLCKEKDGNIVIDFDDVVV
CCCCEEEECCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEHHHHH
RGVTVVREGEITWPAPPIQVSAQPQAAAKKVEAPKEAVKPASPWRKYALMALAIILFGWL
HCEEEEEECCEECCCCCCEECCCCHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHHHHHH
ANVAPKEFLGHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGHGG
HHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
WVSFLSFIAVLIASINIFGGFTVTQRMLKMFRKG
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MEGKTMRIGVPQERLAQETRAAATPKTVEQLLKLGFSVAVESGAGKLASFDDEAFAEAGA
CCCCEEEECCCHHHHHHHHHHCCCHHHHHHHHHCCHHEEEECCCCCCCCCCHHHHHHCCC
EIVTGDEVWQSDVILKVNAPNDDEIALLNPGTTLISFIWPAQNPQLMEKLAARNINVMAM
EEEECCHHCCCCEEEEEECCCCCCEEEECCCCEEEEEECCCCCHHHHHHHHHCCCCEEEE
DSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAAGKVPPAKVMVIGAGV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECEEEECCCCCCCEEEEEECCH
AGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKEEAGSGDGYAKVMSEAFI
HHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHCCCHHHEEECHHCCCCCCHHHHHHHHHHH
KAEMALFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMKSGSVVVDLASQNGGNCEYT
HHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEE
VPGEVVTTANGVKIIGYTDLPGRLPTQSSQLYGTNLVNLLKLLCKEKDGNIVIDFDDVVV
CCCCEEEECCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEHHHHH
RGVTVVREGEITWPAPPIQVSAQPQAAAKKVEAPKEAVKPASPWRKYALMALAIILFGWL
HCEEEEEECCEECCCCCCEECCCCHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHHHHHH
ANVAPKEFLGHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGHGG
HHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
WVSFLSFIAVLIASINIFGGFTVTQRMLKMFRKG
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3525165; 1633824; 9097039; 9278503; 1932078 [H]