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The map label for this gene is fabI2 [H]

Identifier: 150398634

GI number: 150398634

Start: 3657262

End: 3658068

Strand: Reverse

Name: fabI2 [H]

Synonym: Smed_3448

Alternate gene names: 150398634

Gene position: 3658068-3657262 (Counterclockwise)

Preceding gene: 150398635

Following gene: 150398623

Centisome position: 96.73

GC content: 63.57

Gene sequence:

>807_bases
ATGAACGGATTGATGAACGGAAAGCGCGGCCTCATCATGGGCGTCGCCAACAACCATTCTATTGCCTGGGGGATCGCCAA
GACACTCGCCGCGCAGGGCGCCGAGCTGGCCTTCACCTACCAGGGCGAGGCCCTCGGCAAGCGCGTCAAGCCGCTGGCAG
CAGAGGTCGGTTCCGACTTCCTCCTCCCCTGCGACGTCGAGGACATCGCCTCCGTCGACGCTGTCGTCGCTGCCATGAAG
GAACGCTGGGGCAAGCTCGATTTCGTCGTCCACGCCATTGGCTTCTCGGACAAGAACGAGCTCAAGGGTCTCTATGCCGA
CACCACGCGGGACAATTTCAGCCGCACCATGGTGATCTCCTGCTTCTCCTTCACCGAAATAGCCCGGCGTGCGGCGGAGC
TGATGAGCGATGGCGGCACCATGCTGACGCTCACCTATGGCGGCTCGGTGCGGGTCATGCCGAACTACAATGTCATGGGC
GTTGCCAAGGCCGCGCTCGAGGCGTCGGTGCGCTATCTCGCAGCCGACTACGGAACGCGCGGCATCCGCGTCAATGCGAT
CTCGGCCGGCCCGATCCGGACACTGGCCGGAGCAGGCATTTCCGACGCGCGCGCCATGCTCTCGTGGCAGCAGAAGAATT
CGCCCCTGCGCCGCACCGTCACCATTGAGGACGTCGGCAGTTCCGCACTGTACCTGCTTTCGGATCTCTCGCGCGGCGTC
ACCGGCGAAATCCACTACGTCGACTCCGGCTACAACATCACTTCGATGCCGACGCTCGAGGCATTACGGACCGCAGACGC
GGATTAG

Upstream 100 bases:

>100_bases
CGCATCGACAATGCCAGGATCGACACGGTTCTTTCGAACTCCTTCGGCTTCGGCGGCACCAACGCGACGCTCGTCTTCCA
GCGCTATAACGGATAACACG

Downstream 100 bases:

>100_bases
CCGTCTGCCGGGTTTGACCCGAGGACTAACCCATCCCCTCGAGCGGGGTTGGGGATTTGGCTGCGCCCCTCTCTCCCCTC
GATAGGACGGTGGGGGCGGG

Product: enoyl-(acyl carrier protein) reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase 2 [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MNGLMNGKRGLIMGVANNHSIAWGIAKTLAAQGAELAFTYQGEALGKRVKPLAAEVGSDFLLPCDVEDIASVDAVVAAMK
ERWGKLDFVVHAIGFSDKNELKGLYADTTRDNFSRTMVISCFSFTEIARRAAELMSDGGTMLTLTYGGSVRVMPNYNVMG
VAKAALEASVRYLAADYGTRGIRVNAISAGPIRTLAGAGISDARAMLSWQQKNSPLRRTVTIEDVGSSALYLLSDLSRGV
TGEIHYVDSGYNITSMPTLEALRTADAD

Sequences:

>Translated_268_residues
MNGLMNGKRGLIMGVANNHSIAWGIAKTLAAQGAELAFTYQGEALGKRVKPLAAEVGSDFLLPCDVEDIASVDAVVAAMK
ERWGKLDFVVHAIGFSDKNELKGLYADTTRDNFSRTMVISCFSFTEIARRAAELMSDGGTMLTLTYGGSVRVMPNYNVMG
VAKAALEASVRYLAADYGTRGIRVNAISAGPIRTLAGAGISDARAMLSWQQKNSPLRRTVTIEDVGSSALYLLSDLSRGV
TGEIHYVDSGYNITSMPTLEALRTADAD
>Mature_268_residues
MNGLMNGKRGLIMGVANNHSIAWGIAKTLAAQGAELAFTYQGEALGKRVKPLAAEVGSDFLLPCDVEDIASVDAVVAAMK
ERWGKLDFVVHAIGFSDKNELKGLYADTTRDNFSRTMVISCFSFTEIARRAAELMSDGGTMLTLTYGGSVRVMPNYNVMG
VAKAALEASVRYLAADYGTRGIRVNAISAGPIRTLAGAGISDARAMLSWQQKNSPLRRTVTIEDVGSSALYLLSDLSRGV
TGEIHYVDSGYNITSMPTLEALRTADAD

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787545, Length=259, Percent_Identity=50.965250965251, Blast_Score=264, Evalue=5e-72,
Organism=Escherichia coli, GI87082100, Length=257, Percent_Identity=28.0155642023346, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1789378, Length=258, Percent_Identity=26.3565891472868, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI2367365, Length=253, Percent_Identity=30.0395256916996, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1787905, Length=249, Percent_Identity=27.710843373494, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1787335, Length=251, Percent_Identity=27.0916334661355, Blast_Score=64, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24644337, Length=204, Percent_Identity=29.4117647058824, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 28642; Mature: 28642

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGLMNGKRGLIMGVANNHSIAWGIAKTLAAQGAELAFTYQGEALGKRVKPLAAEVGSDF
CCCCCCCCCEEEEEECCCCEEEHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHCCCCC
LLPCDVEDIASVDAVVAAMKERWGKLDFVVHAIGFSDKNELKGLYADTTRDNFSRTMVIS
EEECCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHCCCEEECCCCCCCCEEEEEE
CFSFTEIARRAAELMSDGGTMLTLTYGGSVRVMPNYNVMGVAKAALEASVRYLAADYGTR
HHHHHHHHHHHHHHHHCCCCEEEEEECCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCC
GIRVNAISAGPIRTLAGAGISDARAMLSWQQKNSPLRRTVTIEDVGSSALYLLSDLSRGV
CEEEEEECCCCHHHHHCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHCCCC
TGEIHYVDSGYNITSMPTLEALRTADAD
CEEEEEEECCCCCCCCCHHHHHHHCCCC
>Mature Secondary Structure
MNGLMNGKRGLIMGVANNHSIAWGIAKTLAAQGAELAFTYQGEALGKRVKPLAAEVGSDF
CCCCCCCCCEEEEEECCCCEEEHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHCCCCC
LLPCDVEDIASVDAVVAAMKERWGKLDFVVHAIGFSDKNELKGLYADTTRDNFSRTMVIS
EEECCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHCCCEEECCCCCCCCEEEEEE
CFSFTEIARRAAELMSDGGTMLTLTYGGSVRVMPNYNVMGVAKAALEASVRYLAADYGTR
HHHHHHHHHHHHHHHHCCCCEEEEEECCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCC
GIRVNAISAGPIRTLAGAGISDARAMLSWQQKNSPLRRTVTIEDVGSSALYLLSDLSRGV
CEEEEEECCCCHHHHHCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHCCCC
TGEIHYVDSGYNITSMPTLEALRTADAD
CEEEEEEECCCCCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]