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The map label for this gene is metA [H]

Identifier: 150398225

GI number: 150398225

Start: 3178464

End: 3179435

Strand: Direct

Name: metA [H]

Synonym: Smed_3030

Alternate gene names: 150398225

Gene position: 3178464-3179435 (Clockwise)

Preceding gene: 150398224

Following gene: 150398226

Centisome position: 84.04

GC content: 58.33

Gene sequence:

>972_bases
ATGGTTTCGCCGCCGGTTTTTTTGTATTTGATCGAGTGGACACCCATGCCCATCAAGATACCCGATACGCTGCCCGCCTT
CGAAACCCTCGTACACGAGGGCGTGCGGCTGATGACCGAAACAGAGGCGATCCGCCAGGACATCCGGCCGCTGCAGATCG
GGCTCTTGAACCTCATGCCGAACAAGATCAAGACCGAGGTCCAGATGGCGCGGCTGATCGGCGCCACGCCGCTGCAGGTC
GAACTGACGCTGGTGCGTGTCAACGGCCACCGGCCGAAAAATACGCCCGAAGAACATCTGCTCGCCTTCTACGAGACCTT
TGAAGAAGTCGAGGCCCGCAAGTTCGACGGCTTCATCATCACCGGCGCGCCCGTCGAGACGCTCGAATATGAAGAGGTCA
CCTACTGGAAGGAACTGCAGCGGATCTTCGACTGGACGACGACCAACGTGCATTCGACGCTGAACGTCTGCTGGGGCGCG
ATGGCTGCCATTTACCATTTTCACGGCATTCCGAAATATCCGCTGAAGGAGAAGGCATTCGGCGTCTACCGCCACCAGAA
CCTCAAGCCCTCCTCGGTCTATCTGAACGGCTTCTCCGACGATTTCGCCGTGCCCGTTTCGCGCTGGACCGAAGTGCGCC
GCGCCGATATAGATCGGGTGCCGGATCTCGAAATCCTGATGGAATCCAAGGAGATGGGCGTCTGCCTCGTGCACGAGAAG
AAAGGCAACCGGCTTTATATGTTCAACCACGTCGAATATGATTCAACGTCGCTGTCAGAGGAATATTTCCGCGACGTCGA
TGCCGGCGTGCCGATCAAGCTGCCGCACGACTATTTCCCGCACAACGACTCCGCCCTTCCCCCGCAGAACCGCTGGCGCA
GCCACGCACATCTCTTCTTCGGCAATTGGATCAACGAGATCTACCAGACGACGCCCTACGAACCGGCGAAAATCGGAGCC
GGAGCGTATTGA

Upstream 100 bases:

>100_bases
TATCAAACAATCCGTGGTGATTTGGCCGGCCGGCTTGCAGCCACGTTAAAGAAGTCGCTAAAGGGCCGAGGGAACGATAG
ATTTCCGAGGACCGGTCGCG

Downstream 100 bases:

>100_bases
AGCGGAAAAGTATGACTTTATAGTTGCCGCACCGCCGGAAATGCCGCAGGTTGGCGCCCGATTGCGGAAATGGGGAGACA
AAATGACGACCGACATGGAA

Product: homoserine O-succinyltransferase

Products: NA

Alternate protein names: Homoserine O-transsuccinylase; HTS [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MVSPPVFLYLIEWTPMPIKIPDTLPAFETLVHEGVRLMTETEAIRQDIRPLQIGLLNLMPNKIKTEVQMARLIGATPLQV
ELTLVRVNGHRPKNTPEEHLLAFYETFEEVEARKFDGFIITGAPVETLEYEEVTYWKELQRIFDWTTTNVHSTLNVCWGA
MAAIYHFHGIPKYPLKEKAFGVYRHQNLKPSSVYLNGFSDDFAVPVSRWTEVRRADIDRVPDLEILMESKEMGVCLVHEK
KGNRLYMFNHVEYDSTSLSEEYFRDVDAGVPIKLPHDYFPHNDSALPPQNRWRSHAHLFFGNWINEIYQTTPYEPAKIGA
GAY

Sequences:

>Translated_323_residues
MVSPPVFLYLIEWTPMPIKIPDTLPAFETLVHEGVRLMTETEAIRQDIRPLQIGLLNLMPNKIKTEVQMARLIGATPLQV
ELTLVRVNGHRPKNTPEEHLLAFYETFEEVEARKFDGFIITGAPVETLEYEEVTYWKELQRIFDWTTTNVHSTLNVCWGA
MAAIYHFHGIPKYPLKEKAFGVYRHQNLKPSSVYLNGFSDDFAVPVSRWTEVRRADIDRVPDLEILMESKEMGVCLVHEK
KGNRLYMFNHVEYDSTSLSEEYFRDVDAGVPIKLPHDYFPHNDSALPPQNRWRSHAHLFFGNWINEIYQTTPYEPAKIGA
GAY
>Mature_323_residues
MVSPPVFLYLIEWTPMPIKIPDTLPAFETLVHEGVRLMTETEAIRQDIRPLQIGLLNLMPNKIKTEVQMARLIGATPLQV
ELTLVRVNGHRPKNTPEEHLLAFYETFEEVEARKFDGFIITGAPVETLEYEEVTYWKELQRIFDWTTTNVHSTLNVCWGA
MAAIYHFHGIPKYPLKEKAFGVYRHQNLKPSSVYLNGFSDDFAVPVSRWTEVRRADIDRVPDLEILMESKEMGVCLVHEK
KGNRLYMFNHVEYDSTSLSEEYFRDVDAGVPIKLPHDYFPHNDSALPPQNRWRSHAHLFFGNWINEIYQTTPYEPAKIGA
GAY

Specific function: Methionine biosynthesis; HTS variant; first step. [C]

COG id: COG1897

COG function: function code E; Homoserine trans-succinylase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HTS family [H]

Homologues:

Organism=Escherichia coli, GI1790443, Length=300, Percent_Identity=44.3333333333333, Blast_Score=276, Evalue=1e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005697
- ProDom:   PD037892 [H]

Pfam domain/function: PF04204 HTS [H]

EC number: =2.3.1.46 [H]

Molecular weight: Translated: 37423; Mature: 37423

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSPPVFLYLIEWTPMPIKIPDTLPAFETLVHEGVRLMTETEAIRQDIRPLQIGLLNLMP
CCCCCEEEEEEECCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
NKIKTEVQMARLIGATPLQVELTLVRVNGHRPKNTPEEHLLAFYETFEEVEARKFDGFII
HHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEE
TGAPVETLEYEEVTYWKELQRIFDWTTTNVHSTLNVCWGAMAAIYHFHGIPKYPLKEKAF
ECCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
GVYRHQNLKPSSVYLNGFSDDFAVPVSRWTEVRRADIDRVPDLEILMESKEMGVCLVHEK
HHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCHHHEECCCCCCEEEEEEC
KGNRLYMFNHVEYDSTSLSEEYFRDVDAGVPIKLPHDYFPHNDSALPPQNRWRSHAHLFF
CCCEEEEEEECCCCCCHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCHHHHCCHHEEHH
GNWINEIYQTTPYEPAKIGAGAY
HHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MVSPPVFLYLIEWTPMPIKIPDTLPAFETLVHEGVRLMTETEAIRQDIRPLQIGLLNLMP
CCCCCEEEEEEECCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
NKIKTEVQMARLIGATPLQVELTLVRVNGHRPKNTPEEHLLAFYETFEEVEARKFDGFII
HHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEE
TGAPVETLEYEEVTYWKELQRIFDWTTTNVHSTLNVCWGAMAAIYHFHGIPKYPLKEKAF
ECCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
GVYRHQNLKPSSVYLNGFSDDFAVPVSRWTEVRRADIDRVPDLEILMESKEMGVCLVHEK
HHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCHHHEECCCCCCEEEEEEC
KGNRLYMFNHVEYDSTSLSEEYFRDVDAGVPIKLPHDYFPHNDSALPPQNRWRSHAHLFF
CCCEEEEEEECCCCCCHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCHHHHCCHHEEHH
GNWINEIYQTTPYEPAKIGAGAY
HHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]