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The map label for this gene is proA [H]

Identifier: 150398206

GI number: 150398206

Start: 3159580

End: 3160863

Strand: Direct

Name: proA [H]

Synonym: Smed_3011

Alternate gene names: 150398206

Gene position: 3159580-3160863 (Clockwise)

Preceding gene: 150398205

Following gene: 150398207

Centisome position: 83.54

GC content: 65.81

Gene sequence:

>1284_bases
ATGCTTGAAACGGTCGAGAACGGCGATGACGTTAAGGCGATTATGCTGGAGATCGGGCGCCGCGCCAAAGCGGCCGCTCG
GCCGCTCGCCGTTGCAAGCGCCGAGCGCAAGCATGCGGCACTTGTCGCCGTGGCGGGCGCTCTTGGCGCACGGGCGGCGG
AAATTCTCGCCGCCAACGCGCTCGACCTCGAAAACGCGCGGGAAAGCGGCGTCGCAAACGCCTTCATCGACCGGCTTACG
CTGACGGAAAGCCGTATCCGCGACATGGCGGACGGTATTCGCGCGATCGCCGAACTCAAGGATCCCGTCGGCGAGGTGAT
CGCCGAATGGGATCGCCCGAACGGGCTCCACATCGAGCGGGTGCGTACGCCGCTCGGCGTCATCGGCGTCATTTATGAGA
GCCGGCCGAACGTTACGGCCGATGCGGGCGCGCTCTGCCTCAAAGCGGGAAATGCAGTGATCCTGCGTGGCGGGTCCGAC
AGTTTCCACTCGTCGCGGGCCATCCACGCCTGCCTTGTCGAGGGCCTGAAGGCCGCCGGCCTGCCGGCCGACGCTATCCA
GATGGTGCCGGTCGCCGACCGCGCCGCCGTCGGCGCCATGCTCACCGGTTTGAATGGCGCGGTCGACGTGATCGTGCCGC
GCGGCGGCAAGAGCCTGGTTGCGCGCGTCCAGAACGAGGCGCGGGTGCCGGTCTTCGCGCATCTCGAAGGCCTCTGCCAC
ATCTATGTCGATGCGTCGGCCGATCTCGCCATGGCAACGCAGATCGTCGTCAATGCCAAGATGCGCCGTACCGGCATCTG
CGGCGCCGCCGAAACGCTGCTGATCGACCGCAACGCCGCAGAAAAGCTCGCAAGGCCACTGATCGAAGCGCTCGTTGAGG
CGGGCTGCGAGGTGCGCGCCGAGAGTAGCCTCTCAAGCGTCATGCCTGGCCTGAAATCCGCCACCGACGAAGACTGGGCG
ACCGAATATCTGGACGCGATCATTTCGGTGAAACTGGTCGACGGCATCTCCGGCGCGATCGAGCACATCAATATCTGGTC
CTCGGCCCATACGGAGGCCATAATCGCGGAAGAGCCGAGGGTGGTGGAGCGCTTCTTCTCCGAGATCGACTCGGCGATCC
TCCTGCACAATGCTTCGACGCAGTTCGCCGATGGCGGCGAGTTCGGCATGGGCGGCGAGATCGGCATCGCCACCGGCAAG
ATGCATGCGCGCGGACCCGTCGGCGTGGAGCAGCTCACCTCGTTCAAGTATCGTGTGCGCGGCACGGGACAGGTGCGGCC
CTGA

Upstream 100 bases:

>100_bases
GCCGGCCGCGCGGCCATGGTGCACCGCGACGATCTGGTCATGACGGCTCCATCGGGTGGGCGCCTGGCGGAGGAAAGCGA
CGAGGGAAAGGGCAAGCTCC

Downstream 100 bases:

>100_bases
CGACACCGAGCCAAGTGAATGCGCGCTATCTGAAGATGCCGCATGTCGAGTCCGGAATGACGGTCGGCCTGTTCGGCGGG
TCCTTCAATCCGCCGCATGA

Product: gamma-glutamyl phosphate reductase

Products: NA

Alternate protein names: GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSA dehydrogenase [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MLETVENGDDVKAIMLEIGRRAKAAARPLAVASAERKHAALVAVAGALGARAAEILAANALDLENARESGVANAFIDRLT
LTESRIRDMADGIRAIAELKDPVGEVIAEWDRPNGLHIERVRTPLGVIGVIYESRPNVTADAGALCLKAGNAVILRGGSD
SFHSSRAIHACLVEGLKAAGLPADAIQMVPVADRAAVGAMLTGLNGAVDVIVPRGGKSLVARVQNEARVPVFAHLEGLCH
IYVDASADLAMATQIVVNAKMRRTGICGAAETLLIDRNAAEKLARPLIEALVEAGCEVRAESSLSSVMPGLKSATDEDWA
TEYLDAIISVKLVDGISGAIEHINIWSSAHTEAIIAEEPRVVERFFSEIDSAILLHNASTQFADGGEFGMGGEIGIATGK
MHARGPVGVEQLTSFKYRVRGTGQVRP

Sequences:

>Translated_427_residues
MLETVENGDDVKAIMLEIGRRAKAAARPLAVASAERKHAALVAVAGALGARAAEILAANALDLENARESGVANAFIDRLT
LTESRIRDMADGIRAIAELKDPVGEVIAEWDRPNGLHIERVRTPLGVIGVIYESRPNVTADAGALCLKAGNAVILRGGSD
SFHSSRAIHACLVEGLKAAGLPADAIQMVPVADRAAVGAMLTGLNGAVDVIVPRGGKSLVARVQNEARVPVFAHLEGLCH
IYVDASADLAMATQIVVNAKMRRTGICGAAETLLIDRNAAEKLARPLIEALVEAGCEVRAESSLSSVMPGLKSATDEDWA
TEYLDAIISVKLVDGISGAIEHINIWSSAHTEAIIAEEPRVVERFFSEIDSAILLHNASTQFADGGEFGMGGEIGIATGK
MHARGPVGVEQLTSFKYRVRGTGQVRP
>Mature_427_residues
MLETVENGDDVKAIMLEIGRRAKAAARPLAVASAERKHAALVAVAGALGARAAEILAANALDLENARESGVANAFIDRLT
LTESRIRDMADGIRAIAELKDPVGEVIAEWDRPNGLHIERVRTPLGVIGVIYESRPNVTADAGALCLKAGNAVILRGGSD
SFHSSRAIHACLVEGLKAAGLPADAIQMVPVADRAAVGAMLTGLNGAVDVIVPRGGKSLVARVQNEARVPVFAHLEGLCH
IYVDASADLAMATQIVVNAKMRRTGICGAAETLLIDRNAAEKLARPLIEALVEAGCEVRAESSLSSVMPGLKSATDEDWA
TEYLDAIISVKLVDGISGAIEHINIWSSAHTEAIIAEEPRVVERFFSEIDSAILLHNASTQFADGGEFGMGGEIGIATGK
MHARGPVGVEQLTSFKYRVRGTGQVRP

Specific function: Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate [H]

COG id: COG0014

COG function: function code E; Gamma-glutamyl phosphate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyl phosphate reductase family [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=413, Percent_Identity=35.5932203389831, Blast_Score=249, Evalue=4e-66,
Organism=Homo sapiens, GI62912457, Length=413, Percent_Identity=35.5932203389831, Blast_Score=249, Evalue=4e-66,
Organism=Escherichia coli, GI1786438, Length=408, Percent_Identity=41.6666666666667, Blast_Score=315, Evalue=4e-87,
Organism=Caenorhabditis elegans, GI17569967, Length=416, Percent_Identity=35.3365384615385, Blast_Score=251, Evalue=4e-67,
Organism=Caenorhabditis elegans, GI17569969, Length=416, Percent_Identity=35.3365384615385, Blast_Score=251, Evalue=4e-67,
Organism=Saccharomyces cerevisiae, GI6324899, Length=430, Percent_Identity=35.5813953488372, Blast_Score=266, Evalue=6e-72,
Organism=Drosophila melanogaster, GI21357643, Length=411, Percent_Identity=33.0900243309002, Blast_Score=227, Evalue=1e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR000965
- InterPro:   IPR020593
- InterPro:   IPR012134 [H]

Pfam domain/function: PF00171 Aldedh [H]

EC number: =1.2.1.41 [H]

Molecular weight: Translated: 44915; Mature: 44915

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS01223 PROA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLETVENGDDVKAIMLEIGRRAKAAARPLAVASAERKHAALVAVAGALGARAAEILAANA
CCCCCCCCCHHHHHHHHHCCHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCC
LDLENARESGVANAFIDRLTLTESRIRDMADGIRAIAELKDPVGEVIAEWDRPNGLHIER
CCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHH
VRTPLGVIGVIYESRPNVTADAGALCLKAGNAVILRGGSDSFHSSRAIHACLVEGLKAAG
HHCCHHEEEEEEECCCCCCCCCCHHEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHCC
LPADAIQMVPVADRAAVGAMLTGLNGAVDVIVPRGGKSLVARVQNEARVPVFAHLEGLCH
CCHHHEEECCCHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHCCCCCCHHEECCCEEE
IYVDASADLAMATQIVVNAKMRRTGICGAAETLLIDRNAAEKLARPLIEALVEAGCEVRA
EEEECCCCHHHHHHHHHHHHHHHCCCCCCHHEEEEECCHHHHHHHHHHHHHHHCCCCEEC
ESSLSSVMPGLKSATDEDWATEYLDAIISVKLVDGISGAIEHINIWSSAHTEAIIAEEPR
HHHHHHHCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCEEECCCCH
VVERFFSEIDSAILLHNASTQFADGGEFGMGGEIGIATGKMHARGPVGVEQLTSFKYRVR
HHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHCCEEEEE
GTGQVRP
CCCCCCC
>Mature Secondary Structure
MLETVENGDDVKAIMLEIGRRAKAAARPLAVASAERKHAALVAVAGALGARAAEILAANA
CCCCCCCCCHHHHHHHHHCCHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCC
LDLENARESGVANAFIDRLTLTESRIRDMADGIRAIAELKDPVGEVIAEWDRPNGLHIER
CCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHH
VRTPLGVIGVIYESRPNVTADAGALCLKAGNAVILRGGSDSFHSSRAIHACLVEGLKAAG
HHCCHHEEEEEEECCCCCCCCCCHHEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHCC
LPADAIQMVPVADRAAVGAMLTGLNGAVDVIVPRGGKSLVARVQNEARVPVFAHLEGLCH
CCHHHEEECCCHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHCCCCCCHHEECCCEEE
IYVDASADLAMATQIVVNAKMRRTGICGAAETLLIDRNAAEKLARPLIEALVEAGCEVRA
EEEECCCCHHHHHHHHHHHHHHHCCCCCCHHEEEEECCHHHHHHHHHHHHHHHCCCCEEC
ESSLSSVMPGLKSATDEDWATEYLDAIISVKLVDGISGAIEHINIWSSAHTEAIIAEEPR
HHHHHHHCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCEEECCCCH
VVERFFSEIDSAILLHNASTQFADGGEFGMGGEIGIATGKMHARGPVGVEQLTSFKYRVR
HHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHCCEEEEE
GTGQVRP
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]