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The map label for this gene is proB1 [H]

Identifier: 150398205

GI number: 150398205

Start: 3158406

End: 3159587

Strand: Direct

Name: proB1 [H]

Synonym: Smed_3010

Alternate gene names: 150398205

Gene position: 3158406-3159587 (Clockwise)

Preceding gene: 150398204

Following gene: 150398206

Centisome position: 83.51

GC content: 66.84

Gene sequence:

>1182_bases
ATGACCAAGGCCCGCAAAGCGCTCGATAAATACCGCCGGGTCGTGATCAAGATAGGCTCGGCGCTGCTCGTCGACCGCAA
GACGGGTTTGAAGAAGAGCTGGCTCGACGCCATGTGCGCCGATATCGCGGCCCTCAGGGCCAAGGGCGTGGAGGTGCTCG
TCGTCTCCTCCGGGGCGATCGCGCTCGGACGCACGGTGCTCGACCTTTCCGCGGGTGCCTTGAAGCTCGAAGAGAGCCAG
GCGGCCGCCGCCGTCGGCCAGATCGTGCTGGCGCGTGCCTGGTCGGAGAGCCTCTCCACCCATGCGATCGTCGCCGGCCA
GATCCTTCTGACGCTCGGCGACACCGAGGAGCGCCGGCGCTACCTCAATGCCCGCGCGACGATCGGCCAGCTTCTGAGGC
TTGGCTCCGTGCCGATCATCAACGAGAATGACACGGTCGCCACGACCGAAATCCGCTATGGCGACAACGATCGGCTCGCA
GCGCGGGTCGCGACCATGGTCGGCGCCGATCTTCTGGTCCTGCTTTCGGATATAGACGGGCTCTATACGGCACCGCCGCA
TCTCGATCCGAATGCCCGCCTTCTTGAAACCGTCGCCGAAATCACGCCCGACATAGAGGCAATGGCAGGCGGCGCAGCCT
CGGAACTCTCGCGCGGCGGAATGCGCACCAAGATCGACGCCGGCAAGATCGCCACCACGGCCGGCTGCGCGATGATCATC
GCCTCCGGCAAGCCAGAGCATCCGCTGGCAGCGGTGGAAGCAGGTGGGCGCTCCTCCTGGTTCGCCCCCGCCGGCTCTCC
AGTAACGGCCCGAAAAACCTGGATCGCAGGGCAGCTTCTGCCGGCCGGTTCGCTCTCGATCGACGCCGGGGCGGAAACGG
CGCTCAGATCCGGCAAGAGCCTGCTGCCGGCCGGTGTCCGGCAGGTGACGGGAAGCTTCGGCCGCGGCGATACCATTGCC
ATAATCGGCGCCTCAGGGCGCGAGATCGCCCGCGGGCTCGCCGGCTACGACGCCGAGGAGGCGCGCCAGATCGCCGGCAA
AAAATCGGCTGAGATCGCCGTCATTCTCGGCTATGCCGGCCGCGCGGCCATGGTGCACCGCGACGATCTGGTCATGACGG
CTCCATCGGGTGGGCGCCTGGCGGAGGAAAGCGACGAGGGAAAGGGCAAGCTCCATGCTTGA

Upstream 100 bases:

>100_bases
TGCTTATCTCCGCCGTCACCAACAAGGGCATGACGGAAACACTGAGAGCATTGCGCAGCGTCATCGACGCGGCCAAGGCG
GGCGAGGAGGACGCCTGAGC

Downstream 100 bases:

>100_bases
AACGGTCGAGAACGGCGATGACGTTAAGGCGATTATGCTGGAGATCGGGCGCCGCGCCAAAGCGGCCGCTCGGCCGCTCG
CCGTTGCAAGCGCCGAGCGC

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase 1; GK 1 [H]

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MTKARKALDKYRRVVIKIGSALLVDRKTGLKKSWLDAMCADIAALRAKGVEVLVVSSGAIALGRTVLDLSAGALKLEESQ
AAAAVGQIVLARAWSESLSTHAIVAGQILLTLGDTEERRRYLNARATIGQLLRLGSVPIINENDTVATTEIRYGDNDRLA
ARVATMVGADLLVLLSDIDGLYTAPPHLDPNARLLETVAEITPDIEAMAGGAASELSRGGMRTKIDAGKIATTAGCAMII
ASGKPEHPLAAVEAGGRSSWFAPAGSPVTARKTWIAGQLLPAGSLSIDAGAETALRSGKSLLPAGVRQVTGSFGRGDTIA
IIGASGREIARGLAGYDAEEARQIAGKKSAEIAVILGYAGRAAMVHRDDLVMTAPSGGRLAEESDEGKGKLHA

Sequences:

>Translated_393_residues
MTKARKALDKYRRVVIKIGSALLVDRKTGLKKSWLDAMCADIAALRAKGVEVLVVSSGAIALGRTVLDLSAGALKLEESQ
AAAAVGQIVLARAWSESLSTHAIVAGQILLTLGDTEERRRYLNARATIGQLLRLGSVPIINENDTVATTEIRYGDNDRLA
ARVATMVGADLLVLLSDIDGLYTAPPHLDPNARLLETVAEITPDIEAMAGGAASELSRGGMRTKIDAGKIATTAGCAMII
ASGKPEHPLAAVEAGGRSSWFAPAGSPVTARKTWIAGQLLPAGSLSIDAGAETALRSGKSLLPAGVRQVTGSFGRGDTIA
IIGASGREIARGLAGYDAEEARQIAGKKSAEIAVILGYAGRAAMVHRDDLVMTAPSGGRLAEESDEGKGKLHA
>Mature_392_residues
TKARKALDKYRRVVIKIGSALLVDRKTGLKKSWLDAMCADIAALRAKGVEVLVVSSGAIALGRTVLDLSAGALKLEESQA
AAAVGQIVLARAWSESLSTHAIVAGQILLTLGDTEERRRYLNARATIGQLLRLGSVPIINENDTVATTEIRYGDNDRLAA
RVATMVGADLLVLLSDIDGLYTAPPHLDPNARLLETVAEITPDIEAMAGGAASELSRGGMRTKIDAGKIATTAGCAMIIA
SGKPEHPLAAVEAGGRSSWFAPAGSPVTARKTWIAGQLLPAGSLSIDAGAETALRSGKSLLPAGVRQVTGSFGRGDTIAI
IGASGREIARGLAGYDAEEARQIAGKKSAEIAVILGYAGRAAMVHRDDLVMTAPSGGRLAEESDEGKGKLHA

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=306, Percent_Identity=30.718954248366, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI62912457, Length=304, Percent_Identity=30.9210526315789, Blast_Score=122, Evalue=7e-28,
Organism=Escherichia coli, GI1786437, Length=360, Percent_Identity=42.5, Blast_Score=255, Evalue=4e-69,
Organism=Caenorhabditis elegans, GI17569969, Length=259, Percent_Identity=33.2046332046332, Blast_Score=127, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI17569967, Length=259, Percent_Identity=33.2046332046332, Blast_Score=127, Evalue=9e-30,
Organism=Saccharomyces cerevisiae, GI6321822, Length=417, Percent_Identity=32.3741007194245, Blast_Score=182, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6320506, Length=418, Percent_Identity=32.0574162679426, Blast_Score=178, Evalue=2e-45,
Organism=Drosophila melanogaster, GI21357643, Length=297, Percent_Identity=30.976430976431, Blast_Score=114, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 40739; Mature: 40608

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKARKALDKYRRVVIKIGSALLVDRKTGLKKSWLDAMCADIAALRAKGVEVLVVSSGAI
CCHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCEEEEEEECCCH
ALGRTVLDLSAGALKLEESQAAAAVGQIVLARAWSESLSTHAIVAGQILLTLGDTEERRR
HHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCHHHHH
YLNARATIGQLLRLGSVPIINENDTVATTEIRYGDNDRLAARVATMVGADLLVLLSDIDG
HHHHHHHHHHHHHHCCCCEECCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCC
LYTAPPHLDPNARLLETVAEITPDIEAMAGGAASELSRGGMRTKIDAGKIATTAGCAMII
CCCCCCCCCCCHHHHHHHHHHCCCHHHHCCCCHHHHHCCCCEEEECCCCEEHHCCEEEEE
ASGKPEHPLAAVEAGGRSSWFAPAGSPVTARKTWIAGQLLPAGSLSIDAGAETALRSGKS
ECCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHEEECCCCCCCCCEECCCHHHHHHCCCC
LLPAGVRQVTGSFGRGDTIAIIGASGREIARGLAGYDAEEARQIAGKKSAEIAVILGYAG
CCHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHCCCCHHHHHHHHCCCCCCEEEEEECCC
RAAMVHRDDLVMTAPSGGRLAEESDEGKGKLHA
CEEEEECCCEEEECCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TKARKALDKYRRVVIKIGSALLVDRKTGLKKSWLDAMCADIAALRAKGVEVLVVSSGAI
CHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCEEEEEEECCCH
ALGRTVLDLSAGALKLEESQAAAAVGQIVLARAWSESLSTHAIVAGQILLTLGDTEERRR
HHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCHHHHH
YLNARATIGQLLRLGSVPIINENDTVATTEIRYGDNDRLAARVATMVGADLLVLLSDIDG
HHHHHHHHHHHHHHCCCCEECCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCC
LYTAPPHLDPNARLLETVAEITPDIEAMAGGAASELSRGGMRTKIDAGKIATTAGCAMII
CCCCCCCCCCCHHHHHHHHHHCCCHHHHCCCCHHHHHCCCCEEEECCCCEEHHCCEEEEE
ASGKPEHPLAAVEAGGRSSWFAPAGSPVTARKTWIAGQLLPAGSLSIDAGAETALRSGKS
ECCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHEEECCCCCCCCCEECCCHHHHHHCCCC
LLPAGVRQVTGSFGRGDTIAIIGASGREIARGLAGYDAEEARQIAGKKSAEIAVILGYAG
CCHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHCCCCHHHHHHHHCCCCCCEEEEEECCC
RAAMVHRDDLVMTAPSGGRLAEESDEGKGKLHA
CEEEEECCCEEEECCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]