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The map label for this gene is ruvC

Identifier: 150397837

GI number: 150397837

Start: 2740691

End: 2741194

Strand: Reverse

Name: ruvC

Synonym: Smed_2639

Alternate gene names: 150397837

Gene position: 2741194-2740691 (Counterclockwise)

Preceding gene: 150397839

Following gene: 150397836

Centisome position: 72.48

GC content: 61.51

Gene sequence:

>504_bases
ATGATTCGCATCATAGGCGTAGATCCAGGCCTAAGGCGCACCGGTTGGGGAATAATTGAGACTCTCGGCAATTCTCTGCG
TTTCATCGCTTCGGGAACGATCACGTCCGATGGCGACATGGACCTTGCCTCGCGCCTCTGCCAATTGCATGACGGGCTTG
CCGAGGTGGTCCACAGCTATCAGCCGCACGAGGCGGCGGTCGAGCAGACCTTCGTCAACAAGGACGCGACCGCCACACTG
AAGCTCGGCCAGGCGCGTGGCATTGCAATGCTGGTGCCGGCCCGGGCAGGCCTGAGGGTGGCGGAATATGCCCCCAACGC
CGTGAAGAAGGCGGTAATCGGTGTGGGCCACGGCGAAAAGCAGCAGATTCACATGATGCTGAAGGTGCTGATGCCCAAGG
CGGAATTCAAAGGCAACGACGCGGCCGACGCGCTCGCCATCGCCATCTGCCATGCCCATAACAGGCAGGCGATTACCAGC
CGCCTCGCAGCGCTTGCAGGGTAG

Upstream 100 bases:

>100_bases
CTTTTGGTCAGCGAGAAGCCATTTCTGTGTAATGTTTTTGTTTTGTTCACTTTTCGCTGGCAGCGTTCCCCTGACCGAAA
TAGGGTGAAGCATGCAGAAT

Downstream 100 bases:

>100_bases
CCCGGCCATCGGTACTGCTTCAAAGGCGACGGATACCCAACCCCATCCAGAGAGACCAGATGATCGGCAAGCTGAAAGGC
ACCATAGACGAGATCGGCGA

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC

Number of amino acids: Translated: 167; Mature: 167

Protein sequence:

>167_residues
MIRIIGVDPGLRRTGWGIIETLGNSLRFIASGTITSDGDMDLASRLCQLHDGLAEVVHSYQPHEAAVEQTFVNKDATATL
KLGQARGIAMLVPARAGLRVAEYAPNAVKKAVIGVGHGEKQQIHMMLKVLMPKAEFKGNDAADALAIAICHAHNRQAITS
RLAALAG

Sequences:

>Translated_167_residues
MIRIIGVDPGLRRTGWGIIETLGNSLRFIASGTITSDGDMDLASRLCQLHDGLAEVVHSYQPHEAAVEQTFVNKDATATL
KLGQARGIAMLVPARAGLRVAEYAPNAVKKAVIGVGHGEKQQIHMMLKVLMPKAEFKGNDAADALAIAICHAHNRQAITS
RLAALAG
>Mature_167_residues
MIRIIGVDPGLRRTGWGIIETLGNSLRFIASGTITSDGDMDLASRLCQLHDGLAEVVHSYQPHEAAVEQTFVNKDATATL
KLGQARGIAMLVPARAGLRVAEYAPNAVKKAVIGVGHGEKQQIHMMLKVLMPKAEFKGNDAADALAIAICHAHNRQAITS
RLAALAG

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family

Homologues:

Organism=Escherichia coli, GI1788170, Length=128, Percent_Identity=42.96875, Blast_Score=115, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVC_SINMW (A6UCT9)

Other databases:

- EMBL:   CP000738
- RefSeq:   YP_001328304.1
- ProteinModelPortal:   A6UCT9
- SMR:   A6UCT9
- STRING:   A6UCT9
- GeneID:   5323508
- GenomeReviews:   CP000738_GR
- KEGG:   smd:Smed_2639
- eggNOG:   COG0817
- HOGENOM:   HBG437552
- OMA:   RTGWGVI
- ProtClustDB:   PRK00039
- BioCyc:   SMED366394:SMED_2639-MONOMER
- HAMAP:   MF_00034
- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176
- PRINTS:   PR00696
- TIGRFAMs:   TIGR00228

Pfam domain/function: PF02075 RuvC; SSF53098 RNaseH_fold

EC number: =3.1.22.4

Molecular weight: Translated: 17755; Mature: 17755

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRIIGVDPGLRRTGWGIIETLGNSLRFIASGTITSDGDMDLASRLCQLHDGLAEVVHSY
CEEEEECCCCCCHHCCHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
QPHEAAVEQTFVNKDATATLKLGQARGIAMLVPARAGLRVAEYAPNAVKKAVIGVGHGEK
CCHHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCCCCHHHHCCHHHHHHHHCCCCCCH
QQIHMMLKVLMPKAEFKGNDAADALAIAICHAHNRQAITSRLAALAG
HHHHHHHHHHHCHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MIRIIGVDPGLRRTGWGIIETLGNSLRFIASGTITSDGDMDLASRLCQLHDGLAEVVHSY
CEEEEECCCCCCHHCCHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
QPHEAAVEQTFVNKDATATLKLGQARGIAMLVPARAGLRVAEYAPNAVKKAVIGVGHGEK
CCHHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCCCCHHHHCCHHHHHHHHCCCCCCH
QQIHMMLKVLMPKAEFKGNDAADALAIAICHAHNRQAITSRLAALAG
HHHHHHHHHHHCHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA