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The map label for this gene is ilvD [H]

Identifier: 150397688

GI number: 150397688

Start: 2572435

End: 2574213

Strand: Direct

Name: ilvD [H]

Synonym: Smed_2488

Alternate gene names: 150397688

Gene position: 2572435-2574213 (Clockwise)

Preceding gene: 150397687

Following gene: 150397689

Centisome position: 68.02

GC content: 63.35

Gene sequence:

>1779_bases
ATGGCTGACAAACTCCAGCGCCGCTTGCGCTCGCAGGACTGGTTCGACAATCCGGACCATATCGATCTTACGGCACTCTA
TCTCGAGCGCTTCATGAACTACGGCGTAACGCCGGAGGAGTTGCGTTCCGGCAAGCCCGTCATCGGCATTGCCCAGAGCG
GCAGCGATCTCACCCCATGCAATCGGGTCCACATGGATCTGGCGAAGCGTGTTCGCGACGGCATCCGCGATGCAGGCGGC
ATTCCGATCGAATTCCCGACGCACCCGATCTTCGAGAACTGCAAGCGTCCGACCGCCGCCCTCGACCGCAACCTCGCCTA
TCTGGGCCTGGTGGAAATTCTCTACGGCTATCCGCTGGATGGCGTGGTGCTGACCACCGGCTGCGACAAGACCACGCCGT
CCGCCCTGATGGCGGCTTCGACGGTCGATATTCCGGCGATCGTGCTCTCCGGCGGACCCATGCTCGATGGCTGGCACGAA
GGCGACCTCGTCGGCTCGGGCACGGTGATCTGGCGCATGCGCCGCAAACTGGCGGCAGGCGAGATCGATCGCGAGGAATT
CATGCAGGCCGCGCTCGATTCGGCGCCTTCGGTCGGCCATTGCAACACCATGGGCACGGCTTCGACTATGAACGCCATGG
CCGAAGCGCTCGGCATGTCGCTCACCGGCTGCGGCGCCATCCCCGCTGCCTATCGCGAGCGTGGGCAGATGGCCTATCGT
ACCGGACGCCGCGCCGTGGAACTCGTCATGGAGGACCTGAAGCCCTCCGACATCCTGACCCGCGAAGCCTTTCTCAACGC
TATCCGAGTCAACTCGGCGATCGGCGGGTCGACCAATGCCCAGCCACATCTTGCGGCCATGGCGAAGCATGCGGGCGTGG
AACTTTATCCGGACGACTGGCAGGTTCATGGCTTCGATATCCCGCTGCTCGCCAACATCCAGCCGGCGGGTGCCTATCTC
GGCGAACGCTACCACCGCGCGGGCGGCACACCCGCCATCATGTGGGAACTGCTGCAGGCCGGAAAGCTCGACGGTAGCTG
TCGCACCGTCACGGGAAAATCCATGGCCGAGAACCTCGAGGGACGCGAATCGACGGACCGTGAGGTCATCAGGCGCTTTG
AGGAGCCGCTCAGGGAAAAGGCGGGCTTCCTGGTGCTGAAGGGCAATCTCTTCGACTTCGCGATCATGAAGATGAGCGTG
GTCTCAGACGATTTCAGGAAGCGGTATCTTCAGGAGCCGGGGCGCGAGGGTGTTTTCGAAGGCAAGGCCGTGGTCTTCGA
TGGTTCGGAAGACTATCACAAGCGCATCAACGACCCCCAACTCGACATCGATGAAGACACCATCCTGGTGATCCGCGGTG
CGGGGCCGCTCGGCTGGCCGGGATCGGCGGAAGTCGTGAACATGCAGCCGCCGGATCACCTCCTGAAGCGTGGCATCAAG
AGCCTGCCCACCATCGGTGACGGTCGCCAGTCCGGCACGGCGGACAGCCCCTCCATCCTGAACGCCTCGCCGGAGAGTGC
GGCCGGCGGCGGTCTCGCCTGGTTGCGGAACGGCGATGTGATCCGGATCGATTTCAACCTGGGCCTTTGCGACATGCTGG
TTTCCGACGGCGAGATCGAAAGGCGAAAGGCGGATGGCATACCGGCCGTGCCGTCCGACGCCACGCCCTGGCAGCGTATC
TATCGCAAATCCGTCACCCAGCTTTCCGATGGAGCGGTGCTCGAGGGTGCCGCAGACTTCCGGCAAATCGCCAAAAACAT
GCCGCGCCACAATCACTAG

Upstream 100 bases:

>100_bases
AGGAGTTTGCCGCTTTCGTCGAGTCCGGCGGTGCCGGACGCGACCACAGCGGATTGCTTCTGCATCTCGAAAAGCTCAAC
CGGAGAAACTAGGTTCCGCC

Downstream 100 bases:

>100_bases
CGGGATGAGGAAAATGTGCGCGGCTTCCGCCGCGGTCCCGCGTCTCAACTCGTTTGAATAAATCACGTTCATGGTTTTAG
GTCCGTGCGACCTAAAACCA

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD [H]

Number of amino acids: Translated: 592; Mature: 591

Protein sequence:

>592_residues
MADKLQRRLRSQDWFDNPDHIDLTALYLERFMNYGVTPEELRSGKPVIGIAQSGSDLTPCNRVHMDLAKRVRDGIRDAGG
IPIEFPTHPIFENCKRPTAALDRNLAYLGLVEILYGYPLDGVVLTTGCDKTTPSALMAASTVDIPAIVLSGGPMLDGWHE
GDLVGSGTVIWRMRRKLAAGEIDREEFMQAALDSAPSVGHCNTMGTASTMNAMAEALGMSLTGCGAIPAAYRERGQMAYR
TGRRAVELVMEDLKPSDILTREAFLNAIRVNSAIGGSTNAQPHLAAMAKHAGVELYPDDWQVHGFDIPLLANIQPAGAYL
GERYHRAGGTPAIMWELLQAGKLDGSCRTVTGKSMAENLEGRESTDREVIRRFEEPLREKAGFLVLKGNLFDFAIMKMSV
VSDDFRKRYLQEPGREGVFEGKAVVFDGSEDYHKRINDPQLDIDEDTILVIRGAGPLGWPGSAEVVNMQPPDHLLKRGIK
SLPTIGDGRQSGTADSPSILNASPESAAGGGLAWLRNGDVIRIDFNLGLCDMLVSDGEIERRKADGIPAVPSDATPWQRI
YRKSVTQLSDGAVLEGAADFRQIAKNMPRHNH

Sequences:

>Translated_592_residues
MADKLQRRLRSQDWFDNPDHIDLTALYLERFMNYGVTPEELRSGKPVIGIAQSGSDLTPCNRVHMDLAKRVRDGIRDAGG
IPIEFPTHPIFENCKRPTAALDRNLAYLGLVEILYGYPLDGVVLTTGCDKTTPSALMAASTVDIPAIVLSGGPMLDGWHE
GDLVGSGTVIWRMRRKLAAGEIDREEFMQAALDSAPSVGHCNTMGTASTMNAMAEALGMSLTGCGAIPAAYRERGQMAYR
TGRRAVELVMEDLKPSDILTREAFLNAIRVNSAIGGSTNAQPHLAAMAKHAGVELYPDDWQVHGFDIPLLANIQPAGAYL
GERYHRAGGTPAIMWELLQAGKLDGSCRTVTGKSMAENLEGRESTDREVIRRFEEPLREKAGFLVLKGNLFDFAIMKMSV
VSDDFRKRYLQEPGREGVFEGKAVVFDGSEDYHKRINDPQLDIDEDTILVIRGAGPLGWPGSAEVVNMQPPDHLLKRGIK
SLPTIGDGRQSGTADSPSILNASPESAAGGGLAWLRNGDVIRIDFNLGLCDMLVSDGEIERRKADGIPAVPSDATPWQRI
YRKSVTQLSDGAVLEGAADFRQIAKNMPRHNH
>Mature_591_residues
ADKLQRRLRSQDWFDNPDHIDLTALYLERFMNYGVTPEELRSGKPVIGIAQSGSDLTPCNRVHMDLAKRVRDGIRDAGGI
PIEFPTHPIFENCKRPTAALDRNLAYLGLVEILYGYPLDGVVLTTGCDKTTPSALMAASTVDIPAIVLSGGPMLDGWHEG
DLVGSGTVIWRMRRKLAAGEIDREEFMQAALDSAPSVGHCNTMGTASTMNAMAEALGMSLTGCGAIPAAYRERGQMAYRT
GRRAVELVMEDLKPSDILTREAFLNAIRVNSAIGGSTNAQPHLAAMAKHAGVELYPDDWQVHGFDIPLLANIQPAGAYLG
ERYHRAGGTPAIMWELLQAGKLDGSCRTVTGKSMAENLEGRESTDREVIRRFEEPLREKAGFLVLKGNLFDFAIMKMSVV
SDDFRKRYLQEPGREGVFEGKAVVFDGSEDYHKRINDPQLDIDEDTILVIRGAGPLGWPGSAEVVNMQPPDHLLKRGIKS
LPTIGDGRQSGTADSPSILNASPESAAGGGLAWLRNGDVIRIDFNLGLCDMLVSDGEIERRKADGIPAVPSDATPWQRIY
RKSVTQLSDGAVLEGAADFRQIAKNMPRHNH

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family [H]

Homologues:

Organism=Escherichia coli, GI48994964, Length=555, Percent_Identity=30.2702702702703, Blast_Score=189, Evalue=4e-49,
Organism=Escherichia coli, GI1788157, Length=452, Percent_Identity=30.5309734513274, Blast_Score=169, Evalue=4e-43,
Organism=Escherichia coli, GI2367371, Length=464, Percent_Identity=27.801724137931, Blast_Score=125, Evalue=8e-30,
Organism=Escherichia coli, GI1786464, Length=461, Percent_Identity=28.4164859002169, Blast_Score=121, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6322476, Length=559, Percent_Identity=29.3381037567084, Blast_Score=208, Evalue=2e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558 [H]

Pfam domain/function: PF00920 ILVD_EDD [H]

EC number: =4.2.1.9 [H]

Molecular weight: Translated: 64586; Mature: 64455

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADKLQRRLRSQDWFDNPDHIDLTALYLERFMNYGVTPEELRSGKPVIGIAQSGSDLTPC
CCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHCCCCEEEEECCCCCCCCH
NRVHMDLAKRVRDGIRDAGGIPIEFPTHPIFENCKRPTAALDRNLAYLGLVEILYGYPLD
HHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHCCCCHHHHHCCHHHHHHHHHHHCCCCC
GVVLTTGCDKTTPSALMAASTVDIPAIVLSGGPMLDGWHEGDLVGSGTVIWRMRRKLAAG
CEEEECCCCCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
EIDREEFMQAALDSAPSVGHCNTMGTASTMNAMAEALGMSLTGCGAIPAAYRERGQMAYR
CCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHCCHHHHH
TGRRAVELVMEDLKPSDILTREAFLNAIRVNSAIGGSTNAQPHLAAMAKHAGVELYPDDW
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCEECCCCC
QVHGFDIPLLANIQPAGAYLGERYHRAGGTPAIMWELLQAGKLDGSCRTVTGKSMAENLE
EEECCCCCEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEECCHHHHHHCC
GRESTDREVIRRFEEPLREKAGFLVLKGNLFDFAIMKMSVVSDDFRKRYLQEPGREGVFE
CCCCHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GKAVVFDGSEDYHKRINDPQLDIDEDTILVIRGAGPLGWPGSAEVVNMQPPDHLLKRGIK
CCEEEECCCHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEECCCHHHHHHHHHH
SLPTIGDGRQSGTADSPSILNASPESAAGGGLAWLRNGDVIRIDFNLGLCDMLVSDGEIE
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEECCCHHHHHHCCCCHH
RRKADGIPAVPSDATPWQRIYRKSVTQLSDGAVLEGAADFRQIAKNMPRHNH
HHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
ADKLQRRLRSQDWFDNPDHIDLTALYLERFMNYGVTPEELRSGKPVIGIAQSGSDLTPC
CHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHCCCCEEEEECCCCCCCCH
NRVHMDLAKRVRDGIRDAGGIPIEFPTHPIFENCKRPTAALDRNLAYLGLVEILYGYPLD
HHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHCCCCHHHHHCCHHHHHHHHHHHCCCCC
GVVLTTGCDKTTPSALMAASTVDIPAIVLSGGPMLDGWHEGDLVGSGTVIWRMRRKLAAG
CEEEECCCCCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
EIDREEFMQAALDSAPSVGHCNTMGTASTMNAMAEALGMSLTGCGAIPAAYRERGQMAYR
CCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHCCHHHHH
TGRRAVELVMEDLKPSDILTREAFLNAIRVNSAIGGSTNAQPHLAAMAKHAGVELYPDDW
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCEECCCCC
QVHGFDIPLLANIQPAGAYLGERYHRAGGTPAIMWELLQAGKLDGSCRTVTGKSMAENLE
EEECCCCCEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEECCHHHHHHCC
GRESTDREVIRRFEEPLREKAGFLVLKGNLFDFAIMKMSVVSDDFRKRYLQEPGREGVFE
CCCCHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GKAVVFDGSEDYHKRINDPQLDIDEDTILVIRGAGPLGWPGSAEVVNMQPPDHLLKRGIK
CCEEEECCCHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEECCCHHHHHHHHHH
SLPTIGDGRQSGTADSPSILNASPESAAGGGLAWLRNGDVIRIDFNLGLCDMLVSDGEIE
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEECCCHHHHHHCCCCHH
RRKADGIPAVPSDATPWQRIYRKSVTQLSDGAVLEGAADFRQIAKNMPRHNH
HHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]