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The map label for this gene is pksJ [H]

Identifier: 150397689

GI number: 150397689

Start: 2574943

End: 2575329

Strand: Direct

Name: pksJ [H]

Synonym: Smed_2490

Alternate gene names: 150397689

Gene position: 2574943-2575329 (Clockwise)

Preceding gene: 150397688

Following gene: 150397690

Centisome position: 68.09

GC content: 61.5

Gene sequence:

>387_bases
ATGCTCGTCGAGGCGGGATGGCAAGGTACGCCAGAACTTAAAATGCTGTGCGGTGGCGAGGCGCTCAACAGTGCCTTGGC
GGCAGATCTTCTCACGCGTGGCGGCGAACTCTGGAATCTCTACGGCCCGACCGAGACGACGATCTGGTCAGCAGCACTTC
GCGTTGAGCCGTCGCTTCTCGATCCCCCAAGCGTGCCAGTGGGCGGCCCGATCTCGAACACGCAGATCTACATTCTGGAC
GCACACGGCGCTCCGGTCCCGATCGGCGTTACCGGCGAGCTTTACATAAGCGGAGCCGGCGTCGCGCGTGGCTATCTGAA
CCGCCCGAAAGGCAGCAAGCCTTTCTCGGCATTGCGCAGATCGGCCCGCCAAGCTATTTTAACATGA

Upstream 100 bases:

>100_bases
GTGATTTCCACTTCATCGTCGCCCTTCTCGAATTTCCACGTCACCATGGTTCCGCCCGGGAAACGGCGACGAATACAAGG
CGACACCGGCCTTATGGCGC

Downstream 100 bases:

>100_bases
ACGAAAATCTCATATTTGGATTCTTCATGCGCTTTGTTGTGTTGGCTTTACTAGGCATCATGCTGTCTTCTCCTGTTTTC
GCTCAATGTGACGGTAGGCT

Product: putative peptide synthetase

Products: pyrophosphate; AMP; enterobactin; pyrophosphate; L-Seryl-AMP [C]

Alternate protein names: PKS [H]

Number of amino acids: Translated: 128; Mature: 128

Protein sequence:

>128_residues
MLVEAGWQGTPELKMLCGGEALNSALAADLLTRGGELWNLYGPTETTIWSAALRVEPSLLDPPSVPVGGPISNTQIYILD
AHGAPVPIGVTGELYISGAGVARGYLNRPKGSKPFSALRRSARQAILT

Sequences:

>Translated_128_residues
MLVEAGWQGTPELKMLCGGEALNSALAADLLTRGGELWNLYGPTETTIWSAALRVEPSLLDPPSVPVGGPISNTQIYILD
AHGAPVPIGVTGELYISGAGVARGYLNRPKGSKPFSALRRSARQAILT
>Mature_128_residues
MLVEAGWQGTPELKMLCGGEALNSALAADLLTRGGELWNLYGPTETTIWSAALRVEPSLLDPPSVPVGGPISNTQIYILD
AHGAPVPIGVTGELYISGAGVARGYLNRPKGSKPFSALRRSARQAILT

Specific function: Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 5 acyl carrier domains [H]

Homologues:

Organism=Escherichia coli, GI1786801, Length=101, Percent_Identity=43.5643564356436, Blast_Score=74, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24648676, Length=100, Percent_Identity=39, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR000794
- InterPro:   IPR001242
- InterPro:   IPR018201
- InterPro:   IPR014031
- InterPro:   IPR014030
- InterPro:   IPR016040
- InterPro:   IPR006163
- InterPro:   IPR020842
- InterPro:   IPR020841
- InterPro:   IPR020807
- InterPro:   IPR013968
- InterPro:   IPR020806
- InterPro:   IPR006162
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF00501 AMP-binding; PF00668 Condensation; PF00109 ketoacyl-synt; PF02801 Ketoacyl-synt_C; PF08659 KR; PF00550 PP-binding [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 13426; Mature: 13426

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVEAGWQGTPELKMLCGGEALNSALAADLLTRGGELWNLYGPTETTIWSAALRVEPSLL
CEECCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEHEEEECCCCC
DPPSVPVGGPISNTQIYILDAHGAPVPIGVTGELYISGAGVARGYLNRPKGSKPFSALRR
CCCCCCCCCCCCCCEEEEEECCCCCEEECCCCEEEEECCCCCCCCCCCCCCCCHHHHHHH
SARQAILT
HHHHHCCC
>Mature Secondary Structure
MLVEAGWQGTPELKMLCGGEALNSALAADLLTRGGELWNLYGPTETTIWSAALRVEPSLL
CEECCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEHEEEECCCCC
DPPSVPVGGPISNTQIYILDAHGAPVPIGVTGELYISGAGVARGYLNRPKGSKPFSALRR
CCCCCCCCCCCCCCEEEEEECCCCCEEECCCCEEEEECCCCCCCCCCCCCCCCHHHHHHH
SARQAILT
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Phosphopantetheine. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 6 ATP; L-serine; 2,3-dihydroxybenzoate [C]

Specific reaction: 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Seryl-AMP 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Ser

General reaction: Transferases; Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7704258; 9384377; 10568751 [H]