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The map label for this gene is lutR [H]

Identifier: 150397684

GI number: 150397684

Start: 2568133

End: 2568849

Strand: Reverse

Name: lutR [H]

Synonym: Smed_2484

Alternate gene names: 150397684

Gene position: 2568849-2568133 (Counterclockwise)

Preceding gene: 150397685

Following gene: 150397683

Centisome position: 67.92

GC content: 61.65

Gene sequence:

>717_bases
ATGGCAACGGATTTCGGACAGATCAGCAGGAGCGAGCACTTGCCTGCGCGCATCGCGGCCAAGATCGGCAGGGAGATCAC
CGAAGGACGTATCGCGCCCGGAGAGAAACTGCCGACCGAAAATCTGCTCGCCACGACCTTCGGGGTCAGCCGTTCGGTGG
TGCGGGAGGCGATAGCGCAATTGCGCAATGAAGGGCTGGTGGAAACCCGCCAGGGCGTCGGCGCCTTCGCTACGGAAATC
GAGCGCCGGCAATCTCTTCGGATAGAGCAGGGAGACCTTGCCAACCGGGGAAGTTTCCGTGATCTCTTTCAGCTTCGTAT
CCCGCTGGAAGTGGAGGCGGCGCGGCTTGCGGCGATCCATCATACACCGGAGGAATTGGGCAGGATCGACGAGGCTTTGC
TGCAGATGACCGGCACGGATAAATGGACGGGCCAGGGGATCGTCGCCGACCTCGCTTTTCACCGCGCGATCGCGGCCGCA
ACCCACAACGACTATTTCCTGCTATTCATAGGTTTCATCGCCGAGCGGATCAGCCTGGCGATCAATGCGGCACGCGCCGC
TGCGATACTGGAGGAGATCGTTGAAGTCACGATCGCGGAACACGTGTCCATTCGAGATGGCGTGGCGGCGCGGGACCCGA
TCAAAGCGGGGGAAGCAATGCGCCATCATCTGAACGGGGCGGCGGCCCGTCTGGACCTGACGATCGAAAGCTTCTAG

Upstream 100 bases:

>100_bases
CCAGCTGCGATTCTAGATACGAACGTGATCTATTCTAATAGATGGGGCGGGATGAGAGCGGACTTTCCTCAATCTGCTCG
AAGCGGGAGAAGCAATCTTG

Downstream 100 bases:

>100_bases
GCGTTGAAGCTTTGCTCGGCTGGCGCCGCCCCCGCTCGCTCCTGGGAGAATATCAGGCTTCACTTACCCACGGCCTAGAC
ATGGCCCAAAAGTCATACTA

Product: GntR domain-containing protein

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 238; Mature: 237

Protein sequence:

>238_residues
MATDFGQISRSEHLPARIAAKIGREITEGRIAPGEKLPTENLLATTFGVSRSVVREAIAQLRNEGLVETRQGVGAFATEI
ERRQSLRIEQGDLANRGSFRDLFQLRIPLEVEAARLAAIHHTPEELGRIDEALLQMTGTDKWTGQGIVADLAFHRAIAAA
THNDYFLLFIGFIAERISLAINAARAAAILEEIVEVTIAEHVSIRDGVAARDPIKAGEAMRHHLNGAAARLDLTIESF

Sequences:

>Translated_238_residues
MATDFGQISRSEHLPARIAAKIGREITEGRIAPGEKLPTENLLATTFGVSRSVVREAIAQLRNEGLVETRQGVGAFATEI
ERRQSLRIEQGDLANRGSFRDLFQLRIPLEVEAARLAAIHHTPEELGRIDEALLQMTGTDKWTGQGIVADLAFHRAIAAA
THNDYFLLFIGFIAERISLAINAARAAAILEEIVEVTIAEHVSIRDGVAARDPIKAGEAMRHHLNGAAARLDLTIESF
>Mature_237_residues
ATDFGQISRSEHLPARIAAKIGREITEGRIAPGEKLPTENLLATTFGVSRSVVREAIAQLRNEGLVETRQGVGAFATEIE
RRQSLRIEQGDLANRGSFRDLFQLRIPLEVEAARLAAIHHTPEELGRIDEALLQMTGTDKWTGQGIVADLAFHRAIAAAT
HNDYFLLFIGFIAERISLAINAARAAAILEEIVEVTIAEHVSIRDGVAARDPIKAGEAMRHHLNGAAARLDLTIESF

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: COG2186

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790780, Length=218, Percent_Identity=28.8990825688073, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1789621, Length=211, Percent_Identity=30.8056872037915, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI87082208, Length=227, Percent_Identity=28.6343612334802, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790032, Length=224, Percent_Identity=29.4642857142857, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789353, Length=228, Percent_Identity=29.3859649122807, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI48994955, Length=207, Percent_Identity=26.5700483091787, Blast_Score=64, Evalue=7e-12,
Organism=Escherichia coli, GI1786303, Length=209, Percent_Identity=32.5358851674641, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 26000; Mature: 25869

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATDFGQISRSEHLPARIAAKIGREITEGRIAPGEKLPTENLLATTFGVSRSVVREAIAQ
CCCCCHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LRNEGLVETRQGVGAFATEIERRQSLRIEQGDLANRGSFRDLFQLRIPLEVEAARLAAIH
HHHCCCHHHHHCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHC
HTPEELGRIDEALLQMTGTDKWTGQGIVADLAFHRAIAAATHNDYFLLFIGFIAERISLA
CCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
INAARAAAILEEIVEVTIAEHVSIRDGVAARDPIKAGEAMRHHLNGAAARLDLTIESF
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHEEEEEEEECC
>Mature Secondary Structure 
ATDFGQISRSEHLPARIAAKIGREITEGRIAPGEKLPTENLLATTFGVSRSVVREAIAQ
CCCCHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LRNEGLVETRQGVGAFATEIERRQSLRIEQGDLANRGSFRDLFQLRIPLEVEAARLAAIH
HHHCCCHHHHHCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHC
HTPEELGRIDEALLQMTGTDKWTGQGIVADLAFHRAIAAATHNDYFLLFIGFIAERISLA
CCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
INAARAAAILEEIVEVTIAEHVSIRDGVAARDPIKAGEAMRHHLNGAAARLDLTIESF
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]