BacMap

Definition	Sinorhizobium medicae WSM419 chromosome, complete genome.
Accession	NC_009636
Length	3,781,904

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The map label for this gene is crt [H]

Identifier: 150397406

GI number: 150397406

Start: 2286083

End: 2286874

Strand: Direct

Name: crt [H]

Synonym: Smed_2206

Alternate gene names: 150397406

Gene position: 2286083-2286874 (Clockwise)

Preceding gene: 150397402

Following gene: 150397409

Centisome position: 60.45

GC content: 64.27

Gene sequence:

>792_bases
ATGTCCGATACTGTGCTTCTGAAAATTTCGGGCGGAACCGCCCTCCTGACGCTCAACCGGCCCGAAAGGCTGAATGCGCT
CAATTACGAGCTGATCGACCGGCTTTTGACCCTCCTCGATCGCCTCGAGGTGGACGCCAAGGTGCATGCCGTCATCCTCA
CCGGAGCGGGTGATCGAGCCTTTTCGGCAGGCGGCGACATTCACGAATTCTCCCACAGCGTGGCCGAAGGCGTGAACGCC
GCGATCCGCGATTTCGTGCGAAGAGGGCAGCAGCTTACCAGCCGTCTCGAGGCCTTTCCGAAGCCTGTGATCGCCGCCGT
CAACGGCATCGCCTATGGCGGCGGCTGCGAAATCACGGAGGCGGTGCATCTCGCGATCGCGAGCGACCGGTCGCGATTTG
CCAAGCCGGAGATCAAGCTTGGCATGCCGCCCACTTTCGGCGGCACGCAGAGACTGCCGCGCCTTGCCGGACGCAAGCGC
GCGCTGGAGCTACTTCTCACCGGCGACGCGTTTTCGGCTGAGCGCGCGCTGGAGATGGGGCTGGTAAATGCGGTGGTTCC
GCACGCGGATCTTATCGAGACCGCCCGAGCGCTCGCGGCGCGCATTATCAGCCACTCTCCTGTTGCGACCGCCGCTATCA
TCTCGGCCGTGACGCGCGGCCTCAACATGGCGATCGGCGAAGGTCTGCTTTGCGAGAGCGAGCAATTCGCACGAATGGTT
CCGACCCACGACCTCAACGAAGGCCTCGCCGCCTGGAAAGAGCGGCGCGAGCCGCGCTATGCCGGCCGCTGA

Upstream 100 bases:

>100_bases
TTTTGTGTCGCCGCCATAATCCAGGTATACCGACTAGTGAATATACATTCCTTTGTCTCCCACTTAGCCTCCGTTCGTCA
ACACCCGACGGGAGACAAAC

Downstream 100 bases:

>100_bases
GATTGCCCGATCATCAGGTCCGGGCGAAGCCGAGGACGAAGAGCGCCAGAGCAGCGGTCGAAGCGAAGCTGCGCACGTGG
TTCCAGCGGACCCATTCGGT

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: Crotonase [H]

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MSDTVLLKISGGTALLTLNRPERLNALNYELIDRLLTLLDRLEVDAKVHAVILTGAGDRAFSAGGDIHEFSHSVAEGVNA
AIRDFVRRGQQLTSRLEAFPKPVIAAVNGIAYGGGCEITEAVHLAIASDRSRFAKPEIKLGMPPTFGGTQRLPRLAGRKR
ALELLLTGDAFSAERALEMGLVNAVVPHADLIETARALAARIISHSPVATAAIISAVTRGLNMAIGEGLLCESEQFARMV
PTHDLNEGLAAWKERREPRYAGR

Sequences:

>Translated_263_residues
MSDTVLLKISGGTALLTLNRPERLNALNYELIDRLLTLLDRLEVDAKVHAVILTGAGDRAFSAGGDIHEFSHSVAEGVNA
AIRDFVRRGQQLTSRLEAFPKPVIAAVNGIAYGGGCEITEAVHLAIASDRSRFAKPEIKLGMPPTFGGTQRLPRLAGRKR
ALELLLTGDAFSAERALEMGLVNAVVPHADLIETARALAARIISHSPVATAAIISAVTRGLNMAIGEGLLCESEQFARMV
PTHDLNEGLAAWKERREPRYAGR
>Mature_262_residues
SDTVLLKISGGTALLTLNRPERLNALNYELIDRLLTLLDRLEVDAKVHAVILTGAGDRAFSAGGDIHEFSHSVAEGVNAA
IRDFVRRGQQLTSRLEAFPKPVIAAVNGIAYGGGCEITEAVHLAIASDRSRFAKPEIKLGMPPTFGGTQRLPRLAGRKRA
LELLLTGDAFSAERALEMGLVNAVVPHADLIETARALAARIISHSPVATAAIISAVTRGLNMAIGEGLLCESEQFARMVP
THDLNEGLAAWKERREPRYAGR

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=246, Percent_Identity=39.8373983739837, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI70995211, Length=251, Percent_Identity=30.2788844621514, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI4502327, Length=255, Percent_Identity=32.156862745098, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI31542718, Length=247, Percent_Identity=29.5546558704453, Blast_Score=104, Evalue=7e-23,
Organism=Homo sapiens, GI37594469, Length=168, Percent_Identity=37.5, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI37594471, Length=168, Percent_Identity=37.5, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI20127408, Length=294, Percent_Identity=26.530612244898, Blast_Score=98, Evalue=9e-21,
Organism=Homo sapiens, GI150378541, Length=256, Percent_Identity=30.078125, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI68989263, Length=218, Percent_Identity=32.1100917431193, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI260274832, Length=189, Percent_Identity=28.5714285714286, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI45643119, Length=187, Percent_Identity=28.8770053475936, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI260275230, Length=187, Percent_Identity=28.8770053475936, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI213417737, Length=230, Percent_Identity=28.2608695652174, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI157694516, Length=230, Percent_Identity=28.2608695652174, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI25453481, Length=206, Percent_Identity=25.7281553398058, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI51538573, Length=206, Percent_Identity=25.7281553398058, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI4757966, Length=206, Percent_Identity=25.7281553398058, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI51538581, Length=206, Percent_Identity=25.7281553398058, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI4757968, Length=183, Percent_Identity=27.8688524590164, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI49355787, Length=183, Percent_Identity=27.8688524590164, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI221136756, Length=213, Percent_Identity=27.6995305164319, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI221136753, Length=213, Percent_Identity=27.6995305164319, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI221307494, Length=213, Percent_Identity=27.6995305164319, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI62530384, Length=212, Percent_Identity=28.3018867924528, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI157694520, Length=204, Percent_Identity=26.9607843137255, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1787659, Length=249, Percent_Identity=39.3574297188755, Blast_Score=164, Evalue=7e-42,
Organism=Escherichia coli, GI221142681, Length=274, Percent_Identity=34.6715328467153, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1788682, Length=192, Percent_Identity=37.5, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1787660, Length=267, Percent_Identity=34.4569288389513, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI87082183, Length=259, Percent_Identity=31.2741312741313, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1788597, Length=268, Percent_Identity=30.2238805970149, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1790281, Length=240, Percent_Identity=31.25, Blast_Score=107, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI25145438, Length=251, Percent_Identity=39.0438247011952, Blast_Score=160, Evalue=8e-40,
Organism=Caenorhabditis elegans, GI17554946, Length=250, Percent_Identity=36, Blast_Score=155, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17540714, Length=231, Percent_Identity=32.4675324675325, Blast_Score=120, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI17558304, Length=206, Percent_Identity=33.495145631068, Blast_Score=111, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI25144276, Length=189, Percent_Identity=33.3333333333333, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI25144157, Length=198, Percent_Identity=31.8181818181818, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI25144160, Length=198, Percent_Identity=31.8181818181818, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17508953, Length=189, Percent_Identity=33.3333333333333, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17508951, Length=189, Percent_Identity=33.3333333333333, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17560910, Length=214, Percent_Identity=30.3738317757009, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17549921, Length=194, Percent_Identity=31.9587628865979, Blast_Score=94, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17536985, Length=254, Percent_Identity=24.8031496062992, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17535521, Length=245, Percent_Identity=28.1632653061224, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17534483, Length=226, Percent_Identity=26.1061946902655, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17540306, Length=201, Percent_Identity=28.3582089552239, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI25141363, Length=179, Percent_Identity=26.2569832402235, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=235, Percent_Identity=29.3617021276596, Blast_Score=93, Evalue=5e-20,
Organism=Drosophila melanogaster, GI20129971, Length=246, Percent_Identity=36.9918699186992, Blast_Score=157, Evalue=6e-39,
Organism=Drosophila melanogaster, GI24653477, Length=246, Percent_Identity=36.9918699186992, Blast_Score=157, Evalue=6e-39,
Organism=Drosophila melanogaster, GI24653139, Length=255, Percent_Identity=31.7647058823529, Blast_Score=127, Evalue=9e-30,
Organism=Drosophila melanogaster, GI28571729, Length=167, Percent_Identity=36.5269461077844, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI28571730, Length=167, Percent_Identity=36.5269461077844, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI19920382, Length=224, Percent_Identity=31.25, Blast_Score=95, Evalue=6e-20,
Organism=Drosophila melanogaster, GI19922422, Length=261, Percent_Identity=25.2873563218391, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21357171, Length=196, Percent_Identity=28.0612244897959, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24654903, Length=190, Percent_Identity=28.4210526315789, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI19921000, Length=185, Percent_Identity=31.3513513513514, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24583077, Length=185, Percent_Identity=31.3513513513514, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24583079, Length=185, Percent_Identity=31.3513513513514, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24650670, Length=199, Percent_Identity=27.6381909547739, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.55 [H]

Molecular weight: Translated: 28244; Mature: 28113

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTVLLKISGGTALLTLNRPERLNALNYELIDRLLTLLDRLEVDAKVHAVILTGAGDRA
CCCEEEEEECCCEEEEEECCCHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCC
FSAGGDIHEFSHSVAEGVNAAIRDFVRRGQQLTSRLEAFPKPVIAAVNGIAYGGGCEITE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCEECCCCCHHH
AVHLAIASDRSRFAKPEIKLGMPPTFGGTQRLPRLAGRKRALELLLTGDAFSAERALEMG
HHHHHHHCCHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHH
LVNAVVPHADLIETARALAARIISHSPVATAAIISAVTRGLNMAIGEGLLCESEQFARMV
HHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHC
PTHDLNEGLAAWKERREPRYAGR
CCCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SDTVLLKISGGTALLTLNRPERLNALNYELIDRLLTLLDRLEVDAKVHAVILTGAGDRA
CCEEEEEECCCEEEEEECCCHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCC
FSAGGDIHEFSHSVAEGVNAAIRDFVRRGQQLTSRLEAFPKPVIAAVNGIAYGGGCEITE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCEECCCCCHHH
AVHLAIASDRSRFAKPEIKLGMPPTFGGTQRLPRLAGRKRALELLLTGDAFSAERALEMG
HHHHHHHCCHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHH
LVNAVVPHADLIETARALAARIISHSPVATAAIISAVTRGLNMAIGEGLLCESEQFARMV
HHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHC
PTHDLNEGLAAWKERREPRYAGR
CCCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8655474; 11466286 [H]