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The map label for this gene is mdeA [H]

Identifier: 150397402

GI number: 150397402

Start: 2282883

End: 2284166

Strand: Direct

Name: mdeA [H]

Synonym: Smed_2201

Alternate gene names: 150397402

Gene position: 2282883-2284166 (Clockwise)

Preceding gene: 150397400

Following gene: 150397406

Centisome position: 60.36

GC content: 62.23

Gene sequence:

>1284_bases
ATGACCGCGCCGCACCCCTCCAAGACCCATATAGGCAACCACAAACTGCATCCCGAAACGCTGATGCTGAATTACGGATA
TGACCCAGAGCTTTCGGAGGGGGCTGTCAAGCCGCCGATCTTTCTGACCTCGACCTTCGTCTTCCACTCCGCCGAGGAAG
GCCGCGACTTCTTCGATTTCGTGTCCGGCCGGCGCGAGCCGCCCGAAGGCGTCGGTGCCGGCCTCGTTTACTCCCGCTTC
AATCATCCGAACAGCGAGATCGTCGAAGACCGGCTCGCCATCTACGAACGGGCCGAAGCCGGCGCCCTCTTTTCCTCCGG
CATGTCGGCGATCGCCACCACGCTTCTCGCTTTCGTGAGGCCGGGCGATTCAATCCTGCATTCGCAGCCGCTTTACGGCG
GCACGGAAACCCTGCTGGCCAAGACGTTCCTCAATCTCGGTATTTCCGCCACCGGTTTTGCCGATGGTGTCGATGAGGCG
GCCGTCAGCGCCGCGGCGGAAGAAGCGATGAGCAAGGGACGGGTCTCCATGATCCTGATAGAGACCCCGGCCAATCCCAC
GAACAGCCTTGTCGATGTCGCGATGATCCGGCGCATCGCCGAAAGGATCGAGGAAAGACAGGCGCATCGGCCGATCGTTG
CCTGCGACAACACCCTGCTCGGTCCCGTCTTTCAACACCCTATCGAACACGGCGCCGATCTTTCCCTTTATTCGTTGACC
AAGTATGTCGGCGGCCATTCCGATCTCATCGCCGGCGCCGTCCTCGGTTCCAAAGCACTGATCAGACAGGTTAAGGCACT
TCGCGGCTCGATCGGCACGCAACTCGACCCGCATTCCTGCTGGATGATCGGCCGCTCTCTAGAAACCCTGTCCGTGCGAA
TGGAAAAGGCGAACGAAAACGCCCGCATCGTCGCCGAATTCCTGCGCGACCATCCCAAGGTCGAGCACATCCACTATCTG
CCGTTCCACGACCCGGAGGCGCCGGTCGGCCGAGTCTTCGCCGCGCAATGCACGGGCGCGGGATCAACCTTCTCCTTCGA
CATCGCCGGCGGCCAGGAGGCTGCCTTCCGCTTCCTCAACGCACTGCAGATATTCAAGCTGGCCGTCAGTCTCGGAGGCA
CGGAATCGCTCGCGAGCCACCCGGCGGCGATGACGCATTCGGGCGTGCCTATCGAGGTCCGCGCCCGCATCGGTGTGCTC
GAATCCACGATCCGCCTGTCGATCGGCATTGAGCACCCGGACGATCTCGTCGCCGACGTCGCCAACGCGCTGACGACGAT
CTAG

Upstream 100 bases:

>100_bases
GTGCATCAAAAGGAAGTGATCGCAAATAGCAAGGACCCCAGCGGGGCGTTTGTTGTAATTTAATCCGTAGCAGAGTCTGC
CTGCCCCCAAGGAGAAGCCC

Downstream 100 bases:

>100_bases
AGCCTGAGTCGAGACCCGGACCGTCCAGACCGTTCCGGAATTGATTCTCTCGTCTTCCATGCCGCTTCCTGCTGCGTTTT
CGAGTAAAACGGGCATGAAG

Product: methionine gamma-lyase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MTAPHPSKTHIGNHKLHPETLMLNYGYDPELSEGAVKPPIFLTSTFVFHSAEEGRDFFDFVSGRREPPEGVGAGLVYSRF
NHPNSEIVEDRLAIYERAEAGALFSSGMSAIATTLLAFVRPGDSILHSQPLYGGTETLLAKTFLNLGISATGFADGVDEA
AVSAAAEEAMSKGRVSMILIETPANPTNSLVDVAMIRRIAERIEERQAHRPIVACDNTLLGPVFQHPIEHGADLSLYSLT
KYVGGHSDLIAGAVLGSKALIRQVKALRGSIGTQLDPHSCWMIGRSLETLSVRMEKANENARIVAEFLRDHPKVEHIHYL
PFHDPEAPVGRVFAAQCTGAGSTFSFDIAGGQEAAFRFLNALQIFKLAVSLGGTESLASHPAAMTHSGVPIEVRARIGVL
ESTIRLSIGIEHPDDLVADVANALTTI

Sequences:

>Translated_427_residues
MTAPHPSKTHIGNHKLHPETLMLNYGYDPELSEGAVKPPIFLTSTFVFHSAEEGRDFFDFVSGRREPPEGVGAGLVYSRF
NHPNSEIVEDRLAIYERAEAGALFSSGMSAIATTLLAFVRPGDSILHSQPLYGGTETLLAKTFLNLGISATGFADGVDEA
AVSAAAEEAMSKGRVSMILIETPANPTNSLVDVAMIRRIAERIEERQAHRPIVACDNTLLGPVFQHPIEHGADLSLYSLT
KYVGGHSDLIAGAVLGSKALIRQVKALRGSIGTQLDPHSCWMIGRSLETLSVRMEKANENARIVAEFLRDHPKVEHIHYL
PFHDPEAPVGRVFAAQCTGAGSTFSFDIAGGQEAAFRFLNALQIFKLAVSLGGTESLASHPAAMTHSGVPIEVRARIGVL
ESTIRLSIGIEHPDDLVADVANALTTI
>Mature_426_residues
TAPHPSKTHIGNHKLHPETLMLNYGYDPELSEGAVKPPIFLTSTFVFHSAEEGRDFFDFVSGRREPPEGVGAGLVYSRFN
HPNSEIVEDRLAIYERAEAGALFSSGMSAIATTLLAFVRPGDSILHSQPLYGGTETLLAKTFLNLGISATGFADGVDEAA
VSAAAEEAMSKGRVSMILIETPANPTNSLVDVAMIRRIAERIEERQAHRPIVACDNTLLGPVFQHPIEHGADLSLYSLTK
YVGGHSDLIAGAVLGSKALIRQVKALRGSIGTQLDPHSCWMIGRSLETLSVRMEKANENARIVAEFLRDHPKVEHIHYLP
FHDPEAPVGRVFAAQCTGAGSTFSFDIAGGQEAAFRFLNALQIFKLAVSLGGTESLASHPAAMTHSGVPIEVRARIGVLE
STIRLSIGIEHPDDLVADVANALTTI

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=400, Percent_Identity=34.25, Blast_Score=202, Evalue=5e-52,
Organism=Homo sapiens, GI299473759, Length=400, Percent_Identity=33, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI25453487, Length=400, Percent_Identity=30.75, Blast_Score=159, Evalue=4e-39,
Organism=Escherichia coli, GI1790375, Length=406, Percent_Identity=33.9901477832512, Blast_Score=214, Evalue=7e-57,
Organism=Escherichia coli, GI1789383, Length=407, Percent_Identity=25.7985257985258, Blast_Score=127, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17538055, Length=413, Percent_Identity=34.3825665859564, Blast_Score=202, Evalue=3e-52,
Organism=Caenorhabditis elegans, GI17560056, Length=413, Percent_Identity=33.4140435835351, Blast_Score=194, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI71980708, Length=389, Percent_Identity=32.133676092545, Blast_Score=191, Evalue=5e-49,
Organism=Saccharomyces cerevisiae, GI6319307, Length=398, Percent_Identity=33.9195979899498, Blast_Score=192, Evalue=9e-50,
Organism=Saccharomyces cerevisiae, GI6321254, Length=428, Percent_Identity=26.8691588785047, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6323333, Length=436, Percent_Identity=27.5229357798165, Blast_Score=127, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6321904, Length=415, Percent_Identity=28.6746987951807, Blast_Score=123, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6321170, Length=350, Percent_Identity=22, Blast_Score=94, Evalue=6e-20,
Organism=Drosophila melanogaster, GI19922554, Length=398, Percent_Identity=36.1809045226131, Blast_Score=234, Evalue=7e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 45928; Mature: 45797

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPHPSKTHIGNHKLHPETLMLNYGYDPELSEGAVKPPIFLTSTFVFHSAEEGRDFFDF
CCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEHHHHEECCCCCCHHHHH
VSGRREPPEGVGAGLVYSRFNHPNSEIVEDRLAIYERAEAGALFSSGMSAIATTLLAFVR
HCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PGDSILHSQPLYGGTETLLAKTFLNLGISATGFADGVDEAAVSAAAEEAMSKGRVSMILI
CCCHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEE
ETPANPTNSLVDVAMIRRIAERIEERQAHRPIVACDNTLLGPVFQHPIEHGADLSLYSLT
ECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHCCCCCHHHHHH
KYVGGHSDLIAGAVLGSKALIRQVKALRGSIGTQLDPHSCWMIGRSLETLSVRMEKANEN
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHCCCC
ARIVAEFLRDHPKVEHIHYLPFHDPEAPVGRVFAAQCTGAGSTFSFDIAGGQEAAFRFLN
HHHHHHHHHCCCCCCEEEECCCCCCCCCHHHEEEEEECCCCCEEEEEECCCHHHHHHHHH
ALQIFKLAVSLGGTESLASHPAAMTHSGVPIEVRARIGVLESTIRLSIGIEHPDDLVADV
HHHHHHHHHHCCCCHHHHCCCCHHCCCCCCEEEEHHHHHHHEEEEEEECCCCCHHHHHHH
ANALTTI
HHHHHCC
>Mature Secondary Structure 
TAPHPSKTHIGNHKLHPETLMLNYGYDPELSEGAVKPPIFLTSTFVFHSAEEGRDFFDF
CCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEHHHHEECCCCCCHHHHH
VSGRREPPEGVGAGLVYSRFNHPNSEIVEDRLAIYERAEAGALFSSGMSAIATTLLAFVR
HCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PGDSILHSQPLYGGTETLLAKTFLNLGISATGFADGVDEAAVSAAAEEAMSKGRVSMILI
CCCHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEE
ETPANPTNSLVDVAMIRRIAERIEERQAHRPIVACDNTLLGPVFQHPIEHGADLSLYSLT
ECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHCCCCCHHHHHH
KYVGGHSDLIAGAVLGSKALIRQVKALRGSIGTQLDPHSCWMIGRSLETLSVRMEKANEN
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHCCCC
ARIVAEFLRDHPKVEHIHYLPFHDPEAPVGRVFAAQCTGAGSTFSFDIAGGQEAAFRFLN
HHHHHHHHHCCCCCCEEEECCCCCCCCCHHHEEEEEECCCCCEEEEEECCCHHHHHHHHH
ALQIFKLAVSLGGTESLASHPAAMTHSGVPIEVRARIGVLESTIRLSIGIEHPDDLVADV
HHHHHHHHHHCCCCHHHHCCCCHHCCCCCCEEEEHHHHHHHEEEEEEECCCCCHHHHHHH
ANALTTI
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]