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The map label for this gene is cocE [H]

Identifier: 150397385

GI number: 150397385

Start: 2258719

End: 2260716

Strand: Direct

Name: cocE [H]

Synonym: Smed_2184

Alternate gene names: 150397385

Gene position: 2258719-2260716 (Clockwise)

Preceding gene: 150397384

Following gene: 150397387

Centisome position: 59.72

GC content: 63.71

Gene sequence:

>1998_bases
ATGGTCGATCGCAATTTCAGCGTCATCGAAAACGAATGGATCACCCTCAAGGACGGAACCCGGCTTGCCGCTCGCATCTG
GATGCCTGAAGGGACCGAGCAGAGCCCGGTTCCGGCGGTGCTGGAATATCTCCCCTATCGCAAGCGCGACGGCACCTGCG
CAAGGGACGAATCGACCTACCCGGCCTTCGCGGCGGCTGGCATCGCCGGCGTGCGCGTCGATATCCGCGGCTCGGGCGAA
TCCGAGGGCGTGATCGACGGCGAATACACACCGCGCGAATTATCCGACGGCTGCGAAATCATCGAATGGATTGCGGCGCA
GCCCTGGTCGAACGGCAAGGTTGGTATGATGGGCATTTCCTGGGGCGGCTTCAACTGTCTGCAGGTGGCGGCTCTCAAGC
CCGCGGCGCTGAAGGCGGTCATCTCGATCGCCTCGACCGTCGACCGCTACAATGACGACATCCACTACAAGAACGGCTGC
CATCTTTCCGCTCAGCTCTCCTGGGCCGCGACCATGCTCGCCTACCAGTCCCGATCCCCCGATCCGGAACTCGTCGGTGA
GGGCTGGCGGGAGATGTGGCTGCAGCGACTCGAGAACGAGCCCTTCTTCCTGGAGGAATGGCTGGAGCACCAGCGCCGCG
ACGACTTCTGGCGGCATGGCTCGATCTGCGAAGATTTTGAAAGCTTCCAGATCCCGGCGCTGGTGATCGCCGGCTGGGCG
GACGGCTACCGCAACACGCCGGTCAAGGCGGTCGAGGGCTTGGGCGGCAGGGCCAAGGCGCTCATCGGTCCATGGGTCCA
CAAATATCCGCATTTCGCCTGGCCGAAGCCGCGGGCGGACTTTCTCGGAGAGGCGATCCGCTGGTGGAACCGCTGGCTTC
GTGACGAAATGAACGACGCCGAGCAGGTGCCGCAGATGCGCGCCTATATCCTCGACGGCCCAAGACCCTCGGTCAGGCGC
AATGAAGATCCGGGCCGCTGGATCGCCAAGGACATCTGGAGGACGCCGGAGTTGCGGGCCTTCACCATATCGAACGACGG
GAGCCTGGCCGCTCAGCCGGCACCGGGACGCGGGGTCGGCGATGTCTACCTCCGATCGCCGCTCGACACCGGCACTGCTG
CCGGTGAGTATTTCACGCTGAAGCCGGACGCGGAGATGCCCGGCGACCAGCGCATCGATGATGCGGGCTCGCTGACTTTC
GACACACATCCCCTGCTCGAGGCGGAGGATTATCTCGGCCAGCCTGTGCTCGACCTCGAGCTTTCCTGCGCTTCCGACAC
TGCCAATCTCGTCGCGCGGATCGTCGACGTCCACCCGGACGGTACGGCGACCCGCGTCGCCTTCGGCGTCCTCAACCTGG
CGCATCGCAGCGGCAATGCGAAGCCGGTGCCGATGGAGAAAAACAAGAAGACGCATGTTTCGCTGGTGCTCGACGCCTGC
GGCTATCGCTTCCGTGCCGGTCACCGCATCCGTTTGTCGCTGTCGACTTCCTATTGGCCGATAATCCTTCCGTCACCGAC
CGATCCCGGCCTGGCGATCGACACGACCAGCATCTCGCTTTCGCTGCCGCTTCTCGGGGAACATCGCGAGATCGTGGTGC
CGCAGCCCGCCAACCCCGACCCGTTGCCGCATTATATCGAGCATTCGCCTGCCGAAACGCGCCGCACCGTCGAGCGCGAT
ATGACGAAGTGCCTGACGCATTACCGCGTCTACGAGAATACCGGCCTCGTCGAGCATCCCGGCACGGGCAATGCGACCCA
GGACATCCGCGACGAAACCTGGACGATCGCGCCGGACGATCCGAATTCGATGACGGGCGTATCCACCTGGACCTGTGTCG
CGCGCCGTGGGCAGCAATCGCTGAAGACCGTCTCTACCTCCCGCCTCGGCTGTACGGAAACCGAGTGGATCACCTCCGCT
CAGGTCGAGGCGTTCGAGGGTGAAGAGAAGATCTTTGAGAAGAGCTTCGAGAAGCGGATCCGGCGCGATTTCATGTGA

Upstream 100 bases:

>100_bases
GTCTGGTTCGACAGCTAAAAGTGCAATGAGTAGGTGCAGGTGGCTTTTTCCACCGGCACCGCGCATCTCAACAGTCAAGC
ACCGAATGGAGGCGGCCGAC

Downstream 100 bases:

>100_bases
ACGACCGGAGCGGGAAAGCCCCTCATCCGGCTGCGGGGGTTAGTCCTCGGCTTAGACCCGAGGAGAGGGGCAAGTGCCGG
CAAGTCCCGCAACACCCCGC

Product: peptidase S15

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 665; Mature: 665

Protein sequence:

>665_residues
MVDRNFSVIENEWITLKDGTRLAARIWMPEGTEQSPVPAVLEYLPYRKRDGTCARDESTYPAFAAAGIAGVRVDIRGSGE
SEGVIDGEYTPRELSDGCEIIEWIAAQPWSNGKVGMMGISWGGFNCLQVAALKPAALKAVISIASTVDRYNDDIHYKNGC
HLSAQLSWAATMLAYQSRSPDPELVGEGWREMWLQRLENEPFFLEEWLEHQRRDDFWRHGSICEDFESFQIPALVIAGWA
DGYRNTPVKAVEGLGGRAKALIGPWVHKYPHFAWPKPRADFLGEAIRWWNRWLRDEMNDAEQVPQMRAYILDGPRPSVRR
NEDPGRWIAKDIWRTPELRAFTISNDGSLAAQPAPGRGVGDVYLRSPLDTGTAAGEYFTLKPDAEMPGDQRIDDAGSLTF
DTHPLLEAEDYLGQPVLDLELSCASDTANLVARIVDVHPDGTATRVAFGVLNLAHRSGNAKPVPMEKNKKTHVSLVLDAC
GYRFRAGHRIRLSLSTSYWPIILPSPTDPGLAIDTTSISLSLPLLGEHREIVVPQPANPDPLPHYIEHSPAETRRTVERD
MTKCLTHYRVYENTGLVEHPGTGNATQDIRDETWTIAPDDPNSMTGVSTWTCVARRGQQSLKTVSTSRLGCTETEWITSA
QVEAFEGEEKIFEKSFEKRIRRDFM

Sequences:

>Translated_665_residues
MVDRNFSVIENEWITLKDGTRLAARIWMPEGTEQSPVPAVLEYLPYRKRDGTCARDESTYPAFAAAGIAGVRVDIRGSGE
SEGVIDGEYTPRELSDGCEIIEWIAAQPWSNGKVGMMGISWGGFNCLQVAALKPAALKAVISIASTVDRYNDDIHYKNGC
HLSAQLSWAATMLAYQSRSPDPELVGEGWREMWLQRLENEPFFLEEWLEHQRRDDFWRHGSICEDFESFQIPALVIAGWA
DGYRNTPVKAVEGLGGRAKALIGPWVHKYPHFAWPKPRADFLGEAIRWWNRWLRDEMNDAEQVPQMRAYILDGPRPSVRR
NEDPGRWIAKDIWRTPELRAFTISNDGSLAAQPAPGRGVGDVYLRSPLDTGTAAGEYFTLKPDAEMPGDQRIDDAGSLTF
DTHPLLEAEDYLGQPVLDLELSCASDTANLVARIVDVHPDGTATRVAFGVLNLAHRSGNAKPVPMEKNKKTHVSLVLDAC
GYRFRAGHRIRLSLSTSYWPIILPSPTDPGLAIDTTSISLSLPLLGEHREIVVPQPANPDPLPHYIEHSPAETRRTVERD
MTKCLTHYRVYENTGLVEHPGTGNATQDIRDETWTIAPDDPNSMTGVSTWTCVARRGQQSLKTVSTSRLGCTETEWITSA
QVEAFEGEEKIFEKSFEKRIRRDFM
>Mature_665_residues
MVDRNFSVIENEWITLKDGTRLAARIWMPEGTEQSPVPAVLEYLPYRKRDGTCARDESTYPAFAAAGIAGVRVDIRGSGE
SEGVIDGEYTPRELSDGCEIIEWIAAQPWSNGKVGMMGISWGGFNCLQVAALKPAALKAVISIASTVDRYNDDIHYKNGC
HLSAQLSWAATMLAYQSRSPDPELVGEGWREMWLQRLENEPFFLEEWLEHQRRDDFWRHGSICEDFESFQIPALVIAGWA
DGYRNTPVKAVEGLGGRAKALIGPWVHKYPHFAWPKPRADFLGEAIRWWNRWLRDEMNDAEQVPQMRAYILDGPRPSVRR
NEDPGRWIAKDIWRTPELRAFTISNDGSLAAQPAPGRGVGDVYLRSPLDTGTAAGEYFTLKPDAEMPGDQRIDDAGSLTF
DTHPLLEAEDYLGQPVLDLELSCASDTANLVARIVDVHPDGTATRVAFGVLNLAHRSGNAKPVPMEKNKKTHVSLVLDAC
GYRFRAGHRIRLSLSTSYWPIILPSPTDPGLAIDTTSISLSLPLLGEHREIVVPQPANPDPLPHYIEHSPAETRRTVERD
MTKCLTHYRVYENTGLVEHPGTGNATQDIRDETWTIAPDDPNSMTGVSTWTCVARRGQQSLKTVSTSRLGCTETEWITSA
QVEAFEGEEKIFEKSFEKRIRRDFM

Specific function: Hydrolyzes cocaine to benzoate and ecgonine methyl ester, endowing the bacteria with the ability to utilize cocaine as a sole source of carbon and energy for growth, as this bacterium lives in the rhizosphere of coca plants [H]

COG id: COG2936

COG function: function code R; Predicted acyl esterases

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha/beta hydrolase family. Carboxylesterase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005674
- InterPro:   IPR008979
- InterPro:   IPR013736
- InterPro:   IPR000383 [H]

Pfam domain/function: PF02129 Peptidase_S15; PF08530 PepX_C [H]

EC number: NA

Molecular weight: Translated: 74469; Mature: 74469

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDRNFSVIENEWITLKDGTRLAARIWMPEGTEQSPVPAVLEYLPYRKRDGTCARDESTY
CCCCCCEEECCCEEEEECCCEEEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCC
PAFAAAGIAGVRVDIRGSGESEGVIDGEYTPRELSDGCEIIEWIAAQPWSNGKVGMMGIS
CHHHHCCCEEEEEEEECCCCCCCEECCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEC
WGGFNCLQVAALKPAALKAVISIASTVDRYNDDIHYKNGCHLSAQLSWAATMLAYQSRSP
CCCCCHHHEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHHCCCC
DPELVGEGWREMWLQRLENEPFFLEEWLEHQRRDDFWRHGSICEDFESFQIPALVIAGWA
CHHHHHCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEEECC
DGYRNTPVKAVEGLGGRAKALIGPWVHKYPHFAWPKPRADFLGEAIRWWNRWLRDEMNDA
CCCCCCCHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHH
EQVPQMRAYILDGPRPSVRRNEDPGRWIAKDIWRTPELRAFTISNDGSLAAQPAPGRGVG
HHHHHHEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCEEEEEECCCCCEEECCCCCCCCC
DVYLRSPLDTGTAAGEYFTLKPDAEMPGDQRIDDAGSLTFDTHPLLEAEDYLGQPVLDLE
CCEEECCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCEEECCCCCCCCHHHCCCCEEEEE
LSCASDTANLVARIVDVHPDGTATRVAFGVLNLAHRSGNAKPVPMEKNKKTHVSLVLDAC
EECCCHHHHHHHHHHEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECC
GYRFRAGHRIRLSLSTSYWPIILPSPTDPGLAIDTTSISLSLPLLGEHREIVVPQPANPD
CCEECCCCEEEEEECCCCCEEEECCCCCCCCEEEECEEEEEECCCCCCCEEEECCCCCCC
PLPHYIEHSPAETRRTVERDMTKCLTHYRVYENTGLVEHPGTGNATQDIRDETWTIAPDD
CCCHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCEEEECCCC
PNSMTGVSTWTCVARRGQQSLKTVSTSRLGCTETEWITSAQVEAFEGEEKIFEKSFEKRI
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHCCHHHHHHHHHHHHH
RRDFM
HHHCC
>Mature Secondary Structure
MVDRNFSVIENEWITLKDGTRLAARIWMPEGTEQSPVPAVLEYLPYRKRDGTCARDESTY
CCCCCCEEECCCEEEEECCCEEEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCC
PAFAAAGIAGVRVDIRGSGESEGVIDGEYTPRELSDGCEIIEWIAAQPWSNGKVGMMGIS
CHHHHCCCEEEEEEEECCCCCCCEECCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEC
WGGFNCLQVAALKPAALKAVISIASTVDRYNDDIHYKNGCHLSAQLSWAATMLAYQSRSP
CCCCCHHHEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHHCCCC
DPELVGEGWREMWLQRLENEPFFLEEWLEHQRRDDFWRHGSICEDFESFQIPALVIAGWA
CHHHHHCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEEECC
DGYRNTPVKAVEGLGGRAKALIGPWVHKYPHFAWPKPRADFLGEAIRWWNRWLRDEMNDA
CCCCCCCHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHH
EQVPQMRAYILDGPRPSVRRNEDPGRWIAKDIWRTPELRAFTISNDGSLAAQPAPGRGVG
HHHHHHEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCEEEEEECCCCCEEECCCCCCCCC
DVYLRSPLDTGTAAGEYFTLKPDAEMPGDQRIDDAGSLTFDTHPLLEAEDYLGQPVLDLE
CCEEECCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCEEECCCCCCCCHHHCCCCEEEEE
LSCASDTANLVARIVDVHPDGTATRVAFGVLNLAHRSGNAKPVPMEKNKKTHVSLVLDAC
EECCCHHHHHHHHHHEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECC
GYRFRAGHRIRLSLSTSYWPIILPSPTDPGLAIDTTSISLSLPLLGEHREIVVPQPANPD
CCEECCCCEEEEEECCCCCEEEECCCCCCCCEEEECEEEEEECCCCCCCEEEECCCCCCC
PLPHYIEHSPAETRRTVERDMTKCLTHYRVYENTGLVEHPGTGNATQDIRDETWTIAPDD
CCCHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCEEEECCCC
PNSMTGVSTWTCVARRGQQSLKTVSTSRLGCTETEWITSAQVEAFEGEEKIFEKSFEKRI
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHCCHHHHHHHHHHHHH
RRDFM
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10698749; 11742345; 12369817 [H]