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The map label for this gene is oppA [C]

Identifier: 150397384

GI number: 150397384

Start: 2257047

End: 2258636

Strand: Direct

Name: oppA [C]

Synonym: Smed_2183

Alternate gene names: 150397384

Gene position: 2257047-2258636 (Clockwise)

Preceding gene: 150397382

Following gene: 150397385

Centisome position: 59.68

GC content: 61.76

Gene sequence:

>1590_bases
ATGACCGAATTCACGAAGCGTCCCGATGGCCTCATCGTCCCGGCCGGCATCAACCGGCGCGGCTTTCTCGCGGGCACAGC
AGCGCTTGGCATCGCCGCAGGGCTCGGCAGTTCACTCGTAGGCGGTGAAGCGCGGGCGCAGGAACCGAAGCGAGGCGGCC
ATCTGAAGCTCGGCCTCGACGGCGGCGCGACAACCGATTCACTCGACCCGGCAAGCTACAGCGGTTCCGTATCCTTCGTA
ATCGGCCATCTCTGGGGCGACACACTGGTCGAATCCCATCCCGAGACCGGCGCTCCGCTGCCGTCAATCGCTTCCTCCTG
GGAGTCGTCGGCCGACGCTTCGGTCTGGACGTTCAAGATTCGAAAGGACATTGCGTTCCACGATGGCAGGAAACTCGCCG
TTGCAGACGTGATCGCAACCTTGAAGCGGCACTCCGACGAAGGCTCCAAATCGGGCGCGCTGGGACTTATGCGCTCCGTG
AAGGCAATTGAGGAAAAGGCCGGCGATCTCGTCCTGACGCTGACGGAAGGCAATGCCGACCTGCCGCTTCTGCTGACCGA
CTATCACCTCATCATTCAGCCGGACGGCGGCGTCGATAATCCGGCTTCGCCGGTCGGCACCGGACCCTACAAGCTCGCAA
GCTACGAAGCCGGTATACGTGCGACCTTTGAAAAGAACACCGCCGACTGGCGCTCGGACCGCGGCTATGTCGACAGCGTC
GAAATCATCGTCATGAATGACACCACGGCGCGCATTGCGGCGCTTTCCTCCGGGCAGGTGCACTTCATCAACGGCGTCGA
ACCGAAAACCGTGCCGCTGCTCAAGCGCGCGCCCCGCGTGGAAATTCTGCAGACTTCGGGCAAGGGCTTCTACAGCTTCC
TCATGCATTGCGACACGAGCCCCTTCGACAATAACGACCTGAGGCTCGCGCTGAAATATGCGATCGACCGGCAGGCCATT
CTCGACCGTGTGCTCGGCGGCTTTGGCACGCTCGGCAACGATTATCCGGTCAATGAAAACTACGCGCTCGCACCTGAAGG
CATCGAGCAGCGTGCCTACGATCCCGACAAGGCCGCCTTCCACTACAAGAAGTCCGGCCATGACCGGCCGATCCTGCTGC
GCACATCCGACGCCGCCTTCCCGGGCGCGGTCGACGCCTCCGTCCTCTTCCAGGAAAGCGCCCGCAAGGCCGGCATCGAA
ATCGAGGTACGTCGCGAACCGGAAGACGGCTACTGGACCAATGTCTGGAATGTGCAGCCTTTCTGCGCATCCTACTGGGG
CGGCCGCCCCACACAGGACTCCCGCTATTCCACCTCCTATCTTTCGAGTGCGGAATGGAACGACACGCGGTTCAAGCGAG
AGGATTTCGACAAGCTGCTCCTGCAGGCGCGCTCCGAACTGGACGAAGCCAAGCGCAAGGCGCTCTACCATACCATGGCT
CTCATGGTGCGCGATGAAGGCGGCCTCATTCTGCCGGTCTTCAACGACTATGTGAACGCCGCCTCCGCTTCGCTGAAAGG
TTTCGTGCACGACATCGGCAACGACCTTTCGAACGGCTATGTCGGAAGCCGCGTCTGGTTCGACAGCTAA

Upstream 100 bases:

>100_bases
GATAGCGTAAAGCAGCGAAAACCAGCGTCAAGTCGCGAAATTGTCTGGCGCCCGGCAGGCGCTAGATTTCCTCCCAACAA
AACACTCAAGGGGAACTGGA

Downstream 100 bases:

>100_bases
AAGTGCAATGAGTAGGTGCAGGTGGCTTTTTCCACCGGCACCGCGCATCTCAACAGTCAAGCACCGAATGGAGGCGGCCG
ACATGGTCGATCGCAATTTC

Product: extracellular solute-binding protein

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 529; Mature: 528

Protein sequence:

>529_residues
MTEFTKRPDGLIVPAGINRRGFLAGTAALGIAAGLGSSLVGGEARAQEPKRGGHLKLGLDGGATTDSLDPASYSGSVSFV
IGHLWGDTLVESHPETGAPLPSIASSWESSADASVWTFKIRKDIAFHDGRKLAVADVIATLKRHSDEGSKSGALGLMRSV
KAIEEKAGDLVLTLTEGNADLPLLLTDYHLIIQPDGGVDNPASPVGTGPYKLASYEAGIRATFEKNTADWRSDRGYVDSV
EIIVMNDTTARIAALSSGQVHFINGVEPKTVPLLKRAPRVEILQTSGKGFYSFLMHCDTSPFDNNDLRLALKYAIDRQAI
LDRVLGGFGTLGNDYPVNENYALAPEGIEQRAYDPDKAAFHYKKSGHDRPILLRTSDAAFPGAVDASVLFQESARKAGIE
IEVRREPEDGYWTNVWNVQPFCASYWGGRPTQDSRYSTSYLSSAEWNDTRFKREDFDKLLLQARSELDEAKRKALYHTMA
LMVRDEGGLILPVFNDYVNAASASLKGFVHDIGNDLSNGYVGSRVWFDS

Sequences:

>Translated_529_residues
MTEFTKRPDGLIVPAGINRRGFLAGTAALGIAAGLGSSLVGGEARAQEPKRGGHLKLGLDGGATTDSLDPASYSGSVSFV
IGHLWGDTLVESHPETGAPLPSIASSWESSADASVWTFKIRKDIAFHDGRKLAVADVIATLKRHSDEGSKSGALGLMRSV
KAIEEKAGDLVLTLTEGNADLPLLLTDYHLIIQPDGGVDNPASPVGTGPYKLASYEAGIRATFEKNTADWRSDRGYVDSV
EIIVMNDTTARIAALSSGQVHFINGVEPKTVPLLKRAPRVEILQTSGKGFYSFLMHCDTSPFDNNDLRLALKYAIDRQAI
LDRVLGGFGTLGNDYPVNENYALAPEGIEQRAYDPDKAAFHYKKSGHDRPILLRTSDAAFPGAVDASVLFQESARKAGIE
IEVRREPEDGYWTNVWNVQPFCASYWGGRPTQDSRYSTSYLSSAEWNDTRFKREDFDKLLLQARSELDEAKRKALYHTMA
LMVRDEGGLILPVFNDYVNAASASLKGFVHDIGNDLSNGYVGSRVWFDS
>Mature_528_residues
TEFTKRPDGLIVPAGINRRGFLAGTAALGIAAGLGSSLVGGEARAQEPKRGGHLKLGLDGGATTDSLDPASYSGSVSFVI
GHLWGDTLVESHPETGAPLPSIASSWESSADASVWTFKIRKDIAFHDGRKLAVADVIATLKRHSDEGSKSGALGLMRSVK
AIEEKAGDLVLTLTEGNADLPLLLTDYHLIIQPDGGVDNPASPVGTGPYKLASYEAGIRATFEKNTADWRSDRGYVDSVE
IIVMNDTTARIAALSSGQVHFINGVEPKTVPLLKRAPRVEILQTSGKGFYSFLMHCDTSPFDNNDLRLALKYAIDRQAIL
DRVLGGFGTLGNDYPVNENYALAPEGIEQRAYDPDKAAFHYKKSGHDRPILLRTSDAAFPGAVDASVLFQESARKAGIEI
EVRREPEDGYWTNVWNVQPFCASYWGGRPTQDSRYSTSYLSSAEWNDTRFKREDFDKLLLQARSELDEAKRKALYHTMAL
MVRDEGGLILPVFNDYVNAASASLKGFVHDIGNDLSNGYVGSRVWFDS

Specific function: Probably part of an ABC transporter complex that could be involved in peptide import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=549, Percent_Identity=24.2258652094718, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1789966, Length=527, Percent_Identity=20.8728652751423, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1787052, Length=440, Percent_Identity=23.8636363636364, Blast_Score=95, Evalue=9e-21,
Organism=Escherichia coli, GI1787551, Length=331, Percent_Identity=23.2628398791541, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1789887, Length=320, Percent_Identity=24.0625, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 57508; Mature: 57377

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEFTKRPDGLIVPAGINRRGFLAGTAALGIAAGLGSSLVGGEARAQEPKRGGHLKLGLD
CCCCCCCCCCEEEECCCCCCCEEEHHHHHHHHHHCCCHHCCCCHHCCCCCCCCEEEEEEC
GGATTDSLDPASYSGSVSFVIGHLWGDTLVESHPETGAPLPSIASSWESSADASVWTFKI
CCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCEEEEEEE
RKDIAFHDGRKLAVADVIATLKRHSDEGSKSGALGLMRSVKAIEEKAGDLVLTLTEGNAD
EECEEECCCCEEHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCC
LPLLLTDYHLIIQPDGGVDNPASPVGTGPYKLASYEAGIRATFEKNTADWRSDRGYVDSV
CCEEEECEEEEECCCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCCCCCCCCCCEE
EIIVMNDTTARIAALSSGQVHFINGVEPKTVPLLKRAPRVEILQTSGKGFYSFLMHCDTS
EEEEECCCCEEEEEECCCCEEEECCCCCCCCHHHHCCCCEEEEEECCCHHHHHHHHCCCC
PFDNNDLRLALKYAIDRQAILDRVLGGFGTLGNDYPVNENYALAPEGIEQRAYDPDKAAF
CCCCCCEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCHHHCCCCCCHHEE
HYKKSGHDRPILLRTSDAAFPGAVDASVLFQESARKAGIEIEVRREPEDGYWTNVWNVQP
EECCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCEEEECCCCH
FCASYWGGRPTQDSRYSTSYLSSAEWNDTRFKREDFDKLLLQARSELDEAKRKALYHTMA
HHHHHCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEE
LMVRDEGGLILPVFNDYVNAASASLKGFVHDIGNDLSNGYVGSRVWFDS
EEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCEEEECC
>Mature Secondary Structure 
TEFTKRPDGLIVPAGINRRGFLAGTAALGIAAGLGSSLVGGEARAQEPKRGGHLKLGLD
CCCCCCCCCEEEECCCCCCCEEEHHHHHHHHHHCCCHHCCCCHHCCCCCCCCEEEEEEC
GGATTDSLDPASYSGSVSFVIGHLWGDTLVESHPETGAPLPSIASSWESSADASVWTFKI
CCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCEEEEEEE
RKDIAFHDGRKLAVADVIATLKRHSDEGSKSGALGLMRSVKAIEEKAGDLVLTLTEGNAD
EECEEECCCCEEHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCC
LPLLLTDYHLIIQPDGGVDNPASPVGTGPYKLASYEAGIRATFEKNTADWRSDRGYVDSV
CCEEEECEEEEECCCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCCCCCCCCCCEE
EIIVMNDTTARIAALSSGQVHFINGVEPKTVPLLKRAPRVEILQTSGKGFYSFLMHCDTS
EEEEECCCCEEEEEECCCCEEEECCCCCCCCHHHHCCCCEEEEEECCCHHHHHHHHCCCC
PFDNNDLRLALKYAIDRQAILDRVLGGFGTLGNDYPVNENYALAPEGIEQRAYDPDKAAF
CCCCCCEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCHHHCCCCCCHHEE
HYKKSGHDRPILLRTSDAAFPGAVDASVLFQESARKAGIEIEVRREPEDGYWTNVWNVQP
EECCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCEEEECCCCH
FCASYWGGRPTQDSRYSTSYLSSAEWNDTRFKREDFDKLLLQARSELDEAKRKALYHTMA
HHHHHCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEE
LMVRDEGGLILPVFNDYVNAASASLKGFVHDIGNDLSNGYVGSRVWFDS
EEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA