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The map label for this gene is potI [H]

Identifier: 150397371

GI number: 150397371

Start: 2240929

End: 2241747

Strand: Direct

Name: potI [H]

Synonym: Smed_2170

Alternate gene names: 150397371

Gene position: 2240929-2241747 (Clockwise)

Preceding gene: 150397370

Following gene: 150397372

Centisome position: 59.25

GC content: 62.76

Gene sequence:

>819_bases
ATGCGCGCCTTCATCTCGTCGGTCTATCTCTTCCTCTATGCGCCGATCGCCCTCGTCGTGCTGTTCTCGTTCAATGCCGG
CCGCAATGCGAGCGAGTTCGCGGGCTTCTCGACTGCATGGTACGGCAAGGCGCTCGGCAACACCTTCCTCATCTCCGCGC
TGCAGAACAGCCTGCTCATTGCCTTCACCAGCGCCGCACTCGCCGCCGTCTTCGGGACCATGGCGGCGCTCGGCATGGAG
CGGCTCGGCCCCCGCATGCGCGCGCTCTTCGACGCACTCTTCGCAGCCGCGATCGTCGTGCCCGGCGTCGTCATCGGCAT
TGCCACGCTTGTTGCACTGGTTGCGGTGTTCTCCTTCGTCAACCCGGCAATTGCAGCCTTTTGGTCGGGCGATCGACCGC
CGCAGCTAGGGCTCGGTTACGGATCGATCATCGCCGCCCATGGCCTTTTTTCGATGGCGCTCGTCACCATGATCGTCAAG
GCGCGGATCGCGAGCCTCGGCCGCGACATCGTCGAAGCGTCAGGCGATCTCTATGCGACGCCGCTCACCACCTTCCGGCT
CATCGTCCTGCCGCAGATCCTGCCTTCGATCCTCGCAGGCTTCCTCCTCGCTTTCACCTTCTCCTTCGACGACTTCATCA
TCGCCTTCTTCGTCGCCGGTTCGAAGACAACCCTGCCGATCTATGTCTTCGCCTCGATCCGCCGGGGCGTGACGCCGGAA
ATCAATGCGATCGCGACTCTGGTTCTGGTCGCGTCGCTGCTTCTGATCCTGACCGCCCGCGTGCTCATGCGCGAAAAGAA
AAGCAAATCCGGGGAGTAA

Upstream 100 bases:

>100_bases
CGATCGCCGTGACCCTCGTCGCCGTGGTCGTCGTCGTCCTCATGGTCGCGATGCGGGTCGCCTGGAAGGTCGCGGGCACA
AGACAGGTGGATCTCGTCTG

Downstream 100 bases:

>100_bases
AACAATGATTCTGAAAGACCGGATCGCGATCGTGACCGGAGCGGGCTCCGGGATCGGCCAGGCGGGTGCCGCCATCATGG
CGCGCGAAGGCGCGCATGTG

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MRAFISSVYLFLYAPIALVVLFSFNAGRNASEFAGFSTAWYGKALGNTFLISALQNSLLIAFTSAALAAVFGTMAALGME
RLGPRMRALFDALFAAAIVVPGVVIGIATLVALVAVFSFVNPAIAAFWSGDRPPQLGLGYGSIIAAHGLFSMALVTMIVK
ARIASLGRDIVEASGDLYATPLTTFRLIVLPQILPSILAGFLLAFTFSFDDFIIAFFVAGSKTTLPIYVFASIRRGVTPE
INAIATLVLVASLLLILTARVLMREKKSKSGE

Sequences:

>Translated_272_residues
MRAFISSVYLFLYAPIALVVLFSFNAGRNASEFAGFSTAWYGKALGNTFLISALQNSLLIAFTSAALAAVFGTMAALGME
RLGPRMRALFDALFAAAIVVPGVVIGIATLVALVAVFSFVNPAIAAFWSGDRPPQLGLGYGSIIAAHGLFSMALVTMIVK
ARIASLGRDIVEASGDLYATPLTTFRLIVLPQILPSILAGFLLAFTFSFDDFIIAFFVAGSKTTLPIYVFASIRRGVTPE
INAIATLVLVASLLLILTARVLMREKKSKSGE
>Mature_272_residues
MRAFISSVYLFLYAPIALVVLFSFNAGRNASEFAGFSTAWYGKALGNTFLISALQNSLLIAFTSAALAAVFGTMAALGME
RLGPRMRALFDALFAAAIVVPGVVIGIATLVALVAVFSFVNPAIAAFWSGDRPPQLGLGYGSIIAAHGLFSMALVTMIVK
ARIASLGRDIVEASGDLYATPLTTFRLIVLPQILPSILAGFLLAFTFSFDDFIIAFFVAGSKTTLPIYVFASIRRGVTPE
INAIATLVLVASLLLILTARVLMREKKSKSGE

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787081, Length=265, Percent_Identity=41.8867924528302, Blast_Score=158, Evalue=3e-40,
Organism=Escherichia coli, GI1787368, Length=271, Percent_Identity=30.6273062730627, Blast_Score=134, Evalue=6e-33,
Organism=Escherichia coli, GI1787714, Length=262, Percent_Identity=32.0610687022901, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788764, Length=221, Percent_Identity=28.9592760180996, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 28873; Mature: 28873

Theoretical pI: Translated: 10.43; Mature: 10.43

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]