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The map label for this gene is potB [H]

Identifier: 150397370

GI number: 150397370

Start: 2240015

End: 2240929

Strand: Direct

Name: potB [H]

Synonym: Smed_2169

Alternate gene names: 150397370

Gene position: 2240015-2240929 (Clockwise)

Preceding gene: 150397369

Following gene: 150397371

Centisome position: 59.23

GC content: 62.95

Gene sequence:

>915_bases
GTGCCGGCAGGTGATGAGGGGCCACTTCAACGATCAGACGGAAATTCCATGCGCTCTGCAACGAACACGAAAAGGAACCT
GGTGACGGCGGCGCTAGTCGCGCCGGCAGCCGCGTGGCTCGCGGTCTTCCTGGTGCTCCCTTTCATGGCCATGCTCGTCT
TCGCCTTTGGCGAGCGGGCGCCTGAAGGCGGATACCAGGCGGCCTTCACCTTCACGCAGTTCGCCAATCTGCCGACCCGG
GCGGCAGCCTTCTGGAACACCCTGCTCCTGGCACCTGCTGGTGCCCTGCTCTGCCTGCTCATCGCCTATCCGGTCGCCTA
CTACCTCGCCGTCAAGGCAGACCCGCGCTACCGCCTCATCCTCGTTTCGCTCGTCGTCGTTCCGTTCTGGACGAGCCTGC
TCGTGCGTACCTATGCCTGGATGTACATTCTCGGCTCGCGCGGCATTCCCAATCTCCTGTCGATGATCGGTATAGAGGAC
GTCCGAATGCTGAACACGCCCGGCGCCGTGCTGCTCGGCATCGTTTACGGCTACCTGCCGCTGATGATCATGCCGATTTA
TGTGAGCCTCGAAAAACTCGATCGACGGCTGCTGGAGGCCTCTGCCGATCTCGGCGGCAAGCCAGTCTCCACGTTTTTCG
GCGTCACGCTGCCGCTGTCGTTGCCCGGAGTCATGACGGGCGTCGCACTCGTCACCATCCTGCTTCTCGGCGAGTATCTG
ATCCCGCAGCTGCTCGGCGGCGGCAAGGTCTTCTTTCTCGGCAATGCACTGGTAGATCTCTTCCTGCAGTCGCGTAACTG
GCCCTTCGGCTCGGCGATCGCCGTGACCCTCGTCGCCGTGGTCGTCGTCGTCCTCATGGTCGCGATGCGGGTCGCCTGGA
AGGTCGCGGGCACAAGACAGGTGGATCTCGTCTGA

Upstream 100 bases:

>100_bases
GCCGGGCCGAGCTGATGGCGCGCTTCAAATCGGCTTGAGTTGGGAGTGACCGCCCCTCATCCGCCTGCCGGCACCTTCTC
CCCGTAGTGACGGGGAGAAG

Downstream 100 bases:

>100_bases
TGCGCGCCTTCATCTCGTCGGTCTATCTCTTCCTCTATGCGCCGATCGCCCTCGTCGTGCTGTTCTCGTTCAATGCCGGC
CGCAATGCGAGCGAGTTCGC

Product: binding-protein-dependent transport systems inner membrane component

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MPAGDEGPLQRSDGNSMRSATNTKRNLVTAALVAPAAAWLAVFLVLPFMAMLVFAFGERAPEGGYQAAFTFTQFANLPTR
AAAFWNTLLLAPAGALLCLLIAYPVAYYLAVKADPRYRLILVSLVVVPFWTSLLVRTYAWMYILGSRGIPNLLSMIGIED
VRMLNTPGAVLLGIVYGYLPLMIMPIYVSLEKLDRRLLEASADLGGKPVSTFFGVTLPLSLPGVMTGVALVTILLLGEYL
IPQLLGGGKVFFLGNALVDLFLQSRNWPFGSAIAVTLVAVVVVVLMVAMRVAWKVAGTRQVDLV

Sequences:

>Translated_304_residues
MPAGDEGPLQRSDGNSMRSATNTKRNLVTAALVAPAAAWLAVFLVLPFMAMLVFAFGERAPEGGYQAAFTFTQFANLPTR
AAAFWNTLLLAPAGALLCLLIAYPVAYYLAVKADPRYRLILVSLVVVPFWTSLLVRTYAWMYILGSRGIPNLLSMIGIED
VRMLNTPGAVLLGIVYGYLPLMIMPIYVSLEKLDRRLLEASADLGGKPVSTFFGVTLPLSLPGVMTGVALVTILLLGEYL
IPQLLGGGKVFFLGNALVDLFLQSRNWPFGSAIAVTLVAVVVVVLMVAMRVAWKVAGTRQVDLV
>Mature_303_residues
PAGDEGPLQRSDGNSMRSATNTKRNLVTAALVAPAAAWLAVFLVLPFMAMLVFAFGERAPEGGYQAAFTFTQFANLPTRA
AAFWNTLLLAPAGALLCLLIAYPVAYYLAVKADPRYRLILVSLVVVPFWTSLLVRTYAWMYILGSRGIPNLLSMIGIEDV
RMLNTPGAVLLGIVYGYLPLMIMPIYVSLEKLDRRLLEASADLGGKPVSTFFGVTLPLSLPGVMTGVALVTILLLGEYLI
PQLLGGGKVFFLGNALVDLFLQSRNWPFGSAIAVTLVAVVVVVLMVAMRVAWKVAGTRQVDLV

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=266, Percent_Identity=35.7142857142857, Blast_Score=164, Evalue=5e-42,
Organism=Escherichia coli, GI1787080, Length=233, Percent_Identity=39.4849785407725, Blast_Score=164, Evalue=6e-42,
Organism=Escherichia coli, GI1787713, Length=217, Percent_Identity=33.1797235023041, Blast_Score=103, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32798; Mature: 32667

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAGDEGPLQRSDGNSMRSATNTKRNLVTAALVAPAAAWLAVFLVLPFMAMLVFAFGERA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PEGGYQAAFTFTQFANLPTRAAAFWNTLLLAPAGALLCLLIAYPVAYYLAVKADPRYRLI
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
LVSLVVVPFWTSLLVRTYAWMYILGSRGIPNLLSMIGIEDVRMLNTPGAVLLGIVYGYLP
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHCCCCHHHHHHHHHHHHH
LMIMPIYVSLEKLDRRLLEASADLGGKPVSTFFGVTLPLSLPGVMTGVALVTILLLGEYL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
IPQLLGGGKVFFLGNALVDLFLQSRNWPFGSAIAVTLVAVVVVVLMVAMRVAWKVAGTRQ
HHHHHCCCEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
VDLV
ECCC
>Mature Secondary Structure 
PAGDEGPLQRSDGNSMRSATNTKRNLVTAALVAPAAAWLAVFLVLPFMAMLVFAFGERA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC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ECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]