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The map label for this gene is mltB [H]

Identifier: 150397314

GI number: 150397314

Start: 2174863

End: 2176080

Strand: Direct

Name: mltB [H]

Synonym: Smed_2113

Alternate gene names: 150397314

Gene position: 2174863-2176080 (Clockwise)

Preceding gene: 150397313

Following gene: 150397315

Centisome position: 57.51

GC content: 64.45

Gene sequence:

>1218_bases
ATGACAGGCCTAGCGCGCAAAGCCCTTCTCTCCCTCGGATTGCTCGCGGTTGCCGGCGCGCCGGCCCTGGCGCAAGCTCC
GGCTCAGCCACGAAATCCGGCCGTTGCGTGCGGCGGCGATCTCGCCACGTTCCTCGCGGGAATCAAGGCGGAAGCGATTG
CCAAGGGCATCCCCGCAGACGCGGCAGAGCGGGCGCTTGCGGGCGCCGCTATCGACCAGAAAGTGCTGAGCCGCGACCGG
GCGCAAGGGGTATTCAAGCAGACCTTCACGGAGTTCTCGAAGCGAACCGTCAGCAAGTCGCGCCTCGACATCGGTGCACG
GAAGATGCGGGAATATGCCGAAGTCTTTGCCCGGGCCGAGCAGGAATTCGGCGTGCCGGCGCCCGTGATCACCGCGTTTT
GGGCGATGGAAACTGACTTCGGCGCCGTACAGGGTGATTTCAATACGTTGAACGCATTGGTGACGCTCGCACATGACTGC
CGCCGCCCGGAAATGTTCCGGCCGCAACTCCTCGCCGCGATCGAAATGGTTCAGCACGGCGATCTCGATCCCGCGACGAC
GACCGGTGCATGGGCGGGCGAGATCGGCCAGGTGCAGATGCTGCCGGAAGATATCATTGCCTATGGCATCGACGGCGACG
GCGACGGCCATGTCAATCTCAAGACGAGCTCTCCGGATGCGATCCTGACGGCGGCTAAATTCATTCAGAGCCTCGGTTTC
AAGGCCGGCGAGCCGTGGATCCAGGAAGTCCGGGTCCCGGATCAGCTTCCCTGGGAGAAGACGGGCCTGCAGCCGGGAAT
GACGGCCGGCGACTGGTTTGCTCTCGGCGTCACCCCGCGGGACGGCAACACGTCCAACAGTGCGCTCGAAGCTTCGCTCG
TCCTCCCTCAGGGCCGCAAGGGCGCCGCCTTCCTGACCTATCCGAACTTCAACATCTATCTCGAGTGGAACCAGTCCTTC
ATATACACCACCTCCGCCGCCTACTTCGCGACCCGACTGGCCGGCGCCCCCCCTTATCAGCAGGGCAACCCGGAACCCGG
GCTCGGCGACGCCGAGATGAAGGCCCTGCAGACCCGATTGCAGGCACTTGGTCACGACGTCGGCAAGATCGACGGGATCC
TCGGCTCGGGCACGCGTGCTGCGCTGCAGAAGGAACAGCTGAAACTCGGGCTTCCCGCGGACGGTTGGGCAACGCAGAAT
CTCCTGGAGGCGCTCTGA

Upstream 100 bases:

>100_bases
TTCATTCGGTCATAATGCTCTGAGAGAGCACCGGCAGTCACCCTCGACAGAGTTCAGGCCCGGCGGCACTTTGTGCGGCC
GGCCACGGAAAGGAATTGCA

Downstream 100 bases:

>100_bases
GAGCGTTCGGCGTTTTGCTGAGACGCCTTTTCGGGCCAGGCAAATTCGGACAGAATGGCATGCCCGACCTTTCCTGAAGG
CGCTCCGGGATTTCCGCCTA

Product: lytic murein transglycosylase

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MTGLARKALLSLGLLAVAGAPALAQAPAQPRNPAVACGGDLATFLAGIKAEAIAKGIPADAAERALAGAAIDQKVLSRDR
AQGVFKQTFTEFSKRTVSKSRLDIGARKMREYAEVFARAEQEFGVPAPVITAFWAMETDFGAVQGDFNTLNALVTLAHDC
RRPEMFRPQLLAAIEMVQHGDLDPATTTGAWAGEIGQVQMLPEDIIAYGIDGDGDGHVNLKTSSPDAILTAAKFIQSLGF
KAGEPWIQEVRVPDQLPWEKTGLQPGMTAGDWFALGVTPRDGNTSNSALEASLVLPQGRKGAAFLTYPNFNIYLEWNQSF
IYTTSAAYFATRLAGAPPYQQGNPEPGLGDAEMKALQTRLQALGHDVGKIDGILGSGTRAALQKEQLKLGLPADGWATQN
LLEAL

Sequences:

>Translated_405_residues
MTGLARKALLSLGLLAVAGAPALAQAPAQPRNPAVACGGDLATFLAGIKAEAIAKGIPADAAERALAGAAIDQKVLSRDR
AQGVFKQTFTEFSKRTVSKSRLDIGARKMREYAEVFARAEQEFGVPAPVITAFWAMETDFGAVQGDFNTLNALVTLAHDC
RRPEMFRPQLLAAIEMVQHGDLDPATTTGAWAGEIGQVQMLPEDIIAYGIDGDGDGHVNLKTSSPDAILTAAKFIQSLGF
KAGEPWIQEVRVPDQLPWEKTGLQPGMTAGDWFALGVTPRDGNTSNSALEASLVLPQGRKGAAFLTYPNFNIYLEWNQSF
IYTTSAAYFATRLAGAPPYQQGNPEPGLGDAEMKALQTRLQALGHDVGKIDGILGSGTRAALQKEQLKLGLPADGWATQN
LLEAL
>Mature_404_residues
TGLARKALLSLGLLAVAGAPALAQAPAQPRNPAVACGGDLATFLAGIKAEAIAKGIPADAAERALAGAAIDQKVLSRDRA
QGVFKQTFTEFSKRTVSKSRLDIGARKMREYAEVFARAEQEFGVPAPVITAFWAMETDFGAVQGDFNTLNALVTLAHDCR
RPEMFRPQLLAAIEMVQHGDLDPATTTGAWAGEIGQVQMLPEDIIAYGIDGDGDGHVNLKTSSPDAILTAAKFIQSLGFK
AGEPWIQEVRVPDQLPWEKTGLQPGMTAGDWFALGVTPRDGNTSNSALEASLVLPQGRKGAAFLTYPNFNIYLEWNQSFI
YTTSAAYFATRLAGAPPYQQGNPEPGLGDAEMKALQTRLQALGHDVGKIDGILGSGTRAALQKEQLKLGLPADGWATQNL
LEAL

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=235, Percent_Identity=28.936170212766, Blast_Score=88, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 43072; Mature: 42941

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGLARKALLSLGLLAVAGAPALAQAPAQPRNPAVACGGDLATFLAGIKAEAIAKGIPAD
CCCHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCCH
AAERALAGAAIDQKVLSRDRAQGVFKQTFTEFSKRTVSKSRLDIGARKMREYAEVFARAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QEFGVPAPVITAFWAMETDFGAVQGDFNTLNALVTLAHDCRRPEMFRPQLLAAIEMVQHG
HHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCC
DLDPATTTGAWAGEIGQVQMLPEDIIAYGIDGDGDGHVNLKTSSPDAILTAAKFIQSLGF
CCCCCCCCCCCCCCCCEEEECCHHHEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHCC
KAGEPWIQEVRVPDQLPWEKTGLQPGMTAGDWFALGVTPRDGNTSNSALEASLVLPQGRK
CCCCCHHHHCCCCCCCCCHHCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCC
GAAFLTYPNFNIYLEWNQSFIYTTSAAYFATRLAGAPPYQQGNPEPGLGDAEMKALQTRL
CCEEEEECCEEEEEEECCEEEEECHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHH
QALGHDVGKIDGILGSGTRAALQKEQLKLGLPADGWATQNLLEAL
HHHCCCHHHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHC
>Mature Secondary Structure 
TGLARKALLSLGLLAVAGAPALAQAPAQPRNPAVACGGDLATFLAGIKAEAIAKGIPAD
CCHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCCH
AAERALAGAAIDQKVLSRDRAQGVFKQTFTEFSKRTVSKSRLDIGARKMREYAEVFARAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QEFGVPAPVITAFWAMETDFGAVQGDFNTLNALVTLAHDCRRPEMFRPQLLAAIEMVQHG
HHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCC
DLDPATTTGAWAGEIGQVQMLPEDIIAYGIDGDGDGHVNLKTSSPDAILTAAKFIQSLGF
CCCCCCCCCCCCCCCCEEEECCHHHEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHCC
KAGEPWIQEVRVPDQLPWEKTGLQPGMTAGDWFALGVTPRDGNTSNSALEASLVLPQGRK
CCCCCHHHHCCCCCCCCCHHCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCC
GAAFLTYPNFNIYLEWNQSFIYTTSAAYFATRLAGAPPYQQGNPEPGLGDAEMKALQTRL
CCEEEEECCEEEEEEECCEEEEECHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHH
QALGHDVGKIDGILGSGTRAALQKEQLKLGLPADGWATQNLLEAL
HHHCCCHHHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]