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The map label for this gene is murE [H]

Identifier: 150397288

GI number: 150397288

Start: 2145344

End: 2146804

Strand: Reverse

Name: murE [H]

Synonym: Smed_2087

Alternate gene names: 150397288

Gene position: 2146804-2145344 (Counterclockwise)

Preceding gene: 150397289

Following gene: 150397287

Centisome position: 56.77

GC content: 64.89

Gene sequence:

>1461_bases
ATGAAGATCAGGGACCTGGCGGGATCGAGTTTCCCGGAACTTTCGGCGCAGCTGAAGGGCGACGCCGCGAACATCGAGAT
CGGCGGCATTACGGCCGATAGCCGGCAGGTCAGGCCCGGCGACCTCTTCGTCGCGGTCGCCGGAGCGAAGGCGGACGGGT
CCGCTTACATAACGGACGCGCTATCGCGCGGGGCCTCGGCGGTCGTCGCGGGAACCAGCGCCCGGGCAGAGGCTGGCATC
AACGTTTTCACGATCTCCGAACCACGCCGTTTCCTGGCGAAGGCGTCGTCGAGCTTTTTCGGACGCCAGCCCGAGACGAT
GGTGGCGGTCACAGGTACGGCGGGGAAGACGTCCGTTGCCTCCTTCACCCGGCAGATCTGGGCGCATTCCGGTTTCTCGG
CGGCGATGATCGGCACCACGGGCGTCGTATCACCCGGCCGCGCCGAATACGGTTCTCTGACGACACCGGATCCGGTGTCG
CTGCACAAGCTGCTCGCCGAACTGGCTGATGAGGGCGTGACCCACGCCGCAATGGAGGCGTCGAGCCACGGCCTGGACCA
GCAACGGCTGGACGGCGTCAATCTCGCTGCCGCCGCCTTTACGAATCTCGGCCGCGATCACATGGACTACCATCCGACGG
TTGAGCATTACATGGCCTCGAAGATGCGCCTCTTTGCGGCGTTGCTGCCGAAAGGTTCGCCGGCCGTGATCTTCGCCGAT
GACCAATGGTCCGCCGATGCGATCGCTGCCGCTCGCAAGGCAGGGCACGATGTGCGGACCGTTGGGCGCAATGGCGATTT
CATCGCGCTGAAACGTGTCGAGCATTTCCGCCACAAGCAGTCGGCGGAGGTTCATGTCGGCGACGACATCTACGAGATAC
ACGTGCCGCTCGCCGGTGACTTCCAGGTCGCCAATGCACTCGTTGCGGCGGGGCTTGCAATGTCAACGGGGATTTCCGCG
AAGGCAGCTTTCTCGGCGCTGGAGAGGCTGCAGGGCGCCTCAGGGCGCCTCGAACTCGTCGGGCAGACGAAGGATGGCGC
GCTCGCCTACGTGGATTACGCCCACAAGCCGGACGCGCTCTCAAATGTGCTCGAATCCCTGCGTCCTTTTACCACTGGCC
GGGTCGTCGTGGTTTTCGGTTGCGGCGGCGACCGGGACAAGGGCAAGCGCCCGATCATGGGAGAGATCGCCAGCCGCCTT
GCCGATGTCGTCATTGTCACGGACGACAACCCGCGCTCGGAAGTGCCGGAGGTGATCCGCGCCGAGATCATGGCGGCGGC
AAAGGGCGCTACGGAGATTGGCGATCGCGCCGAGGCGATCCGCGTTGCCGTCGGCATGCTGAAGACCGGCGATACGCTGA
TCGTCGCGGGCAAAGGGCATGAAGAGGGGCAGACGGTTGGTTCCGTGACGCTGCCTTTCTCCGACCACGCGGAGGTCCGC
AAGGCACTGGGAGGTCTTTGA

Upstream 100 bases:

>100_bases
AGAGACCATGCTGCATTCTAAAGCCTATGGCGGTCCCTGTGCGTCTGACATGACGCGCAGAGATCCCTGTAGGATCATAT
CGATGTGAGACGTGCAGTTC

Downstream 100 bases:

>100_bases
TTTGAACTGGCTCTGGACAAGCAGCGATCTTCTGGGCGCGATGAACGGCCGTCCCGTCGGGAACCTGCCGGAAGGGATCA
CCGGCATTTCGATCGACAGC

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MKIRDLAGSSFPELSAQLKGDAANIEIGGITADSRQVRPGDLFVAVAGAKADGSAYITDALSRGASAVVAGTSARAEAGI
NVFTISEPRRFLAKASSSFFGRQPETMVAVTGTAGKTSVASFTRQIWAHSGFSAAMIGTTGVVSPGRAEYGSLTTPDPVS
LHKLLAELADEGVTHAAMEASSHGLDQQRLDGVNLAAAAFTNLGRDHMDYHPTVEHYMASKMRLFAALLPKGSPAVIFAD
DQWSADAIAAARKAGHDVRTVGRNGDFIALKRVEHFRHKQSAEVHVGDDIYEIHVPLAGDFQVANALVAAGLAMSTGISA
KAAFSALERLQGASGRLELVGQTKDGALAYVDYAHKPDALSNVLESLRPFTTGRVVVVFGCGGDRDKGKRPIMGEIASRL
ADVVIVTDDNPRSEVPEVIRAEIMAAAKGATEIGDRAEAIRVAVGMLKTGDTLIVAGKGHEEGQTVGSVTLPFSDHAEVR
KALGGL

Sequences:

>Translated_486_residues
MKIRDLAGSSFPELSAQLKGDAANIEIGGITADSRQVRPGDLFVAVAGAKADGSAYITDALSRGASAVVAGTSARAEAGI
NVFTISEPRRFLAKASSSFFGRQPETMVAVTGTAGKTSVASFTRQIWAHSGFSAAMIGTTGVVSPGRAEYGSLTTPDPVS
LHKLLAELADEGVTHAAMEASSHGLDQQRLDGVNLAAAAFTNLGRDHMDYHPTVEHYMASKMRLFAALLPKGSPAVIFAD
DQWSADAIAAARKAGHDVRTVGRNGDFIALKRVEHFRHKQSAEVHVGDDIYEIHVPLAGDFQVANALVAAGLAMSTGISA
KAAFSALERLQGASGRLELVGQTKDGALAYVDYAHKPDALSNVLESLRPFTTGRVVVVFGCGGDRDKGKRPIMGEIASRL
ADVVIVTDDNPRSEVPEVIRAEIMAAAKGATEIGDRAEAIRVAVGMLKTGDTLIVAGKGHEEGQTVGSVTLPFSDHAEVR
KALGGL
>Mature_486_residues
MKIRDLAGSSFPELSAQLKGDAANIEIGGITADSRQVRPGDLFVAVAGAKADGSAYITDALSRGASAVVAGTSARAEAGI
NVFTISEPRRFLAKASSSFFGRQPETMVAVTGTAGKTSVASFTRQIWAHSGFSAAMIGTTGVVSPGRAEYGSLTTPDPVS
LHKLLAELADEGVTHAAMEASSHGLDQQRLDGVNLAAAAFTNLGRDHMDYHPTVEHYMASKMRLFAALLPKGSPAVIFAD
DQWSADAIAAARKAGHDVRTVGRNGDFIALKRVEHFRHKQSAEVHVGDDIYEIHVPLAGDFQVANALVAAGLAMSTGISA
KAAFSALERLQGASGRLELVGQTKDGALAYVDYAHKPDALSNVLESLRPFTTGRVVVVFGCGGDRDKGKRPIMGEIASRL
ADVVIVTDDNPRSEVPEVIRAEIMAAAKGATEIGDRAEAIRVAVGMLKTGDTLIVAGKGHEEGQTVGSVTLPFSDHAEVR
KALGGL

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=504, Percent_Identity=38.2936507936508, Blast_Score=249, Evalue=2e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 50804; Mature: 50804

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRDLAGSSFPELSAQLKGDAANIEIGGITADSRQVRPGDLFVAVAGAKADGSAYITDA
CCCCCCCCCCCCHHHHHHCCCCCEEEEECEECCCCCCCCCCEEEEEECCCCCCCHHHHHH
LSRGASAVVAGTSARAEAGINVFTISEPRRFLAKASSSFFGRQPETMVAVTGTAGKTSVA
HHCCCCEEEECCCCHHHCCCEEEECCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHH
SFTRQIWAHSGFSAAMIGTTGVVSPGRAEYGSLTTPDPVSLHKLLAELADEGVTHAAMEA
HHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHH
SSHGLDQQRLDGVNLAAAAFTNLGRDHMDYHPTVEHYMASKMRLFAALLPKGSPAVIFAD
HHCCCCHHHCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEC
DQWSADAIAAARKAGHDVRTVGRNGDFIALKRVEHFRHKQSAEVHVGDDIYEIHVPLAGD
CCCCHHHHHHHHHCCCCEEEECCCCCEEEEHHHHHHHCCCCCEEEECCCEEEEEECCCCC
FQVANALVAAGLAMSTGISAKAAFSALERLQGASGRLELVGQTKDGALAYVDYAHKPDAL
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEECCCCCHHH
SNVLESLRPFTTGRVVVVFGCGGDRDKGKRPIMGEIASRLADVVIVTDDNPRSEVPEVIR
HHHHHHHCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHEEEEEEECCCCHHHHHHHHH
AEIMAAAKGATEIGDRAEAIRVAVGMLKTGDTLIVAGKGHEEGQTVGSVTLPFSDHAEVR
HHHHHHHCCCHHHHHHHHHHHHHHHHEECCCEEEEECCCCCCCCCEEEEECCCCCHHHHH
KALGGL
HHHCCC
>Mature Secondary Structure
MKIRDLAGSSFPELSAQLKGDAANIEIGGITADSRQVRPGDLFVAVAGAKADGSAYITDA
CCCCCCCCCCCCHHHHHHCCCCCEEEEECEECCCCCCCCCCEEEEEECCCCCCCHHHHHH
LSRGASAVVAGTSARAEAGINVFTISEPRRFLAKASSSFFGRQPETMVAVTGTAGKTSVA
HHCCCCEEEECCCCHHHCCCEEEECCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHH
SFTRQIWAHSGFSAAMIGTTGVVSPGRAEYGSLTTPDPVSLHKLLAELADEGVTHAAMEA
HHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHH
SSHGLDQQRLDGVNLAAAAFTNLGRDHMDYHPTVEHYMASKMRLFAALLPKGSPAVIFAD
HHCCCCHHHCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEC
DQWSADAIAAARKAGHDVRTVGRNGDFIALKRVEHFRHKQSAEVHVGDDIYEIHVPLAGD
CCCCHHHHHHHHHCCCCEEEECCCCCEEEEHHHHHHHCCCCCEEEECCCEEEEEECCCCC
FQVANALVAAGLAMSTGISAKAAFSALERLQGASGRLELVGQTKDGALAYVDYAHKPDAL
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEECCCCCHHH
SNVLESLRPFTTGRVVVVFGCGGDRDKGKRPIMGEIASRLADVVIVTDDNPRSEVPEVIR
HHHHHHHCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHEEEEEEECCCCHHHHHHHHH
AEIMAAAKGATEIGDRAEAIRVAVGMLKTGDTLIVAGKGHEEGQTVGSVTLPFSDHAEVR
HHHHHHHCCCHHHHHHHHHHHHHHHHEECCCEEEEECCCCCCCCCEEEEECCCCCHHHHH
KALGGL
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]