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The map label for this gene is murF [H]

Identifier: 150397287

GI number: 150397287

Start: 2143909

End: 2145342

Strand: Reverse

Name: murF [H]

Synonym: Smed_2086

Alternate gene names: 150397287

Gene position: 2145342-2143909 (Counterclockwise)

Preceding gene: 150397288

Following gene: 150397286

Centisome position: 56.73

GC content: 63.46

Gene sequence:

>1434_bases
TTGAACTGGCTCTGGACAAGCAGCGATCTTCTGGGCGCGATGAACGGCCGTCCCGTCGGGAACCTGCCGGAAGGGATCAC
CGGCATTTCGATCGACAGCCGGACAATCGGCAATGGCGAGGCTTTTTTCGCGATCAGGGGCGATCGCGTCGACGGCCATG
ACTATGCGGGGATCGCCCTTGCTAACGGAGCAGCGCTTCTTGTCGTCAGCGAAGCGAAGCTGCCGGCGCTCGGGCGCCTG
ATCGCCCCGATGATCGTCGTGGACGACGTGCTGGAGGCAATGATCCGGCTGGGTTGCGCGGCACGCGACCGAAGCGCCGC
AAAGATCATTGCCGTTACCGGATCGGTCGGCAAGACCACGACGAAAGAGATGTTGAGGCACATGCTTTCGCCGCTGGGTC
GGGTCCACGCCTCCGTCGCATCCTTCAACAATCACTGGGGCGTGCCCCTGACGCTTGCCCGAATGCCGGAGACGACCGAT
TACGGCGTCTTTGAGATCGGGATGAACCATGCCGGCGAAATTCGGCCGCTGACACGGATGGTTCGTCCGCATGTGGCGGT
CGTGACCAGCATTGCTGCCGCCCATCTCGGGAATTTTGCGAGTCTGGACGACATCGCCGCGGCCAAGGCTGAGATCTTCG
AGGGGGTCGTCAACGGCGGCCATGCGGTGATCAACAGGGACAGTGCTCAATACGAACTGCTGGAGAAGGCCGCCGCGACA
GCCGGGGTCAGCCATGTCCATTTCTTCGGCGCCAATCCAAAATCGGAATTCCGCCTGGCCGAGTTCGCCGGTGGTGCGGA
AGGTTCGGTGCTGTGGGCGGGTGTCGGAGGCAGAACGCTTGAAATCGCGATTGGCGCACCGGGGCGCCATATCGCCGAGA
ACGCTCTGGCGGCGATCGCCGCAGCGACACTGGCAGGCGCGGACACAGATCGCGTCGCCGCATCGCTTGCAACCATGCAG
CCGGAAAAGGGCAGGGGATTGCGGCACCGTCTCAGGATGGGTGCCGGCTACATGACGCTGATCGACGAGAGCTATAACGC
CAACCCGGCTTCAATGCGGGCGGCGATCACGCTGCTCCGCGATGCCGAGCCCTCGCCCGGCGGCCGGCGCATCGCCATTC
TTGGCGACATGCTGGAAATGGGCGAGCATTCGGCGGCGGTTCATGCGGCACTTGCGAGACCCATTGTCGATGCGGGTATC
GCCGACGTCTGGCTGACGGGGACCGAGATGGCACATTTGCGCGATGCCCTGCCGTCGGAGGTGTCCGTCGTCCATCGCGA
TCTGGTGAGCGACCTGACGGCCTACGCTCTCGGCGCAGTTGCAGCCGGCGACGTGGTGATGGTCAAGTCTTCGAAAAGCA
CCGGTTGCGCAAAGATCGTCCAGGCGCTTCTCGATGCCCATCCGCATGACGAGGCCGGAAAAAGGGAAGCATAG

Upstream 100 bases:

>100_bases
GTCGCGGGCAAAGGGCATGAAGAGGGGCAGACGGTTGGTTCCGTGACGCTGCCTTTCTCCGACCACGCGGAGGTCCGCAA
GGCACTGGGAGGTCTTTGAT

Downstream 100 bases:

>100_bases
GGGACGGTGTCGGCCTGCTGCTTCCTTGAAGCGGAACCGATATGACACAACTGGAAGTGCCGCGGCGGATTTTGCGACCC
TGGAAGGGCGCAAGGCGCCG

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MNWLWTSSDLLGAMNGRPVGNLPEGITGISIDSRTIGNGEAFFAIRGDRVDGHDYAGIALANGAALLVVSEAKLPALGRL
IAPMIVVDDVLEAMIRLGCAARDRSAAKIIAVTGSVGKTTTKEMLRHMLSPLGRVHASVASFNNHWGVPLTLARMPETTD
YGVFEIGMNHAGEIRPLTRMVRPHVAVVTSIAAAHLGNFASLDDIAAAKAEIFEGVVNGGHAVINRDSAQYELLEKAAAT
AGVSHVHFFGANPKSEFRLAEFAGGAEGSVLWAGVGGRTLEIAIGAPGRHIAENALAAIAAATLAGADTDRVAASLATMQ
PEKGRGLRHRLRMGAGYMTLIDESYNANPASMRAAITLLRDAEPSPGGRRIAILGDMLEMGEHSAAVHAALARPIVDAGI
ADVWLTGTEMAHLRDALPSEVSVVHRDLVSDLTAYALGAVAAGDVVMVKSSKSTGCAKIVQALLDAHPHDEAGKREA

Sequences:

>Translated_477_residues
MNWLWTSSDLLGAMNGRPVGNLPEGITGISIDSRTIGNGEAFFAIRGDRVDGHDYAGIALANGAALLVVSEAKLPALGRL
IAPMIVVDDVLEAMIRLGCAARDRSAAKIIAVTGSVGKTTTKEMLRHMLSPLGRVHASVASFNNHWGVPLTLARMPETTD
YGVFEIGMNHAGEIRPLTRMVRPHVAVVTSIAAAHLGNFASLDDIAAAKAEIFEGVVNGGHAVINRDSAQYELLEKAAAT
AGVSHVHFFGANPKSEFRLAEFAGGAEGSVLWAGVGGRTLEIAIGAPGRHIAENALAAIAAATLAGADTDRVAASLATMQ
PEKGRGLRHRLRMGAGYMTLIDESYNANPASMRAAITLLRDAEPSPGGRRIAILGDMLEMGEHSAAVHAALARPIVDAGI
ADVWLTGTEMAHLRDALPSEVSVVHRDLVSDLTAYALGAVAAGDVVMVKSSKSTGCAKIVQALLDAHPHDEAGKREA
>Mature_477_residues
MNWLWTSSDLLGAMNGRPVGNLPEGITGISIDSRTIGNGEAFFAIRGDRVDGHDYAGIALANGAALLVVSEAKLPALGRL
IAPMIVVDDVLEAMIRLGCAARDRSAAKIIAVTGSVGKTTTKEMLRHMLSPLGRVHASVASFNNHWGVPLTLARMPETTD
YGVFEIGMNHAGEIRPLTRMVRPHVAVVTSIAAAHLGNFASLDDIAAAKAEIFEGVVNGGHAVINRDSAQYELLEKAAAT
AGVSHVHFFGANPKSEFRLAEFAGGAEGSVLWAGVGGRTLEIAIGAPGRHIAENALAAIAAATLAGADTDRVAASLATMQ
PEKGRGLRHRLRMGAGYMTLIDESYNANPASMRAAITLLRDAEPSPGGRRIAILGDMLEMGEHSAAVHAALARPIVDAGI
ADVWLTGTEMAHLRDALPSEVSVVHRDLVSDLTAYALGAVAAGDVVMVKSSKSTGCAKIVQALLDAHPHDEAGKREA

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=438, Percent_Identity=34.0182648401827, Blast_Score=176, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 49809; Mature: 49809

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNWLWTSSDLLGAMNGRPVGNLPEGITGISIDSRTIGNGEAFFAIRGDRVDGHDYAGIAL
CCCEECCHHHHHCCCCCCCCCCCCCCEEEEECCEECCCCCEEEEEECCCCCCCCCCCEEE
ANGAALLVVSEAKLPALGRLIAPMIVVDDVLEAMIRLGCAARDRSAAKIIAVTGSVGKTT
ECCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCHH
TKEMLRHMLSPLGRVHASVASFNNHWGVPLTLARMPETTDYGVFEIGMNHAGEIRPLTRM
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCEEEECCCCCCCHHHHHHH
VRPHVAVVTSIAAAHLGNFASLDDIAAAKAEIFEGVVNGGHAVINRDSAQYELLEKAAAT
HCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHH
AGVSHVHFFGANPKSEFRLAEFAGGAEGSVLWAGVGGRTLEIAIGAPGRHIAENALAAIA
CCCCEEEEECCCCCCCEEHHHHCCCCCCCEEEEECCCEEEEEEECCCCHHHHHHHHHHHH
AATLAGADTDRVAASLATMQPEKGRGLRHRLRMGAGYMTLIDESYNANPASMRAAITLLR
HHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHH
DAEPSPGGRRIAILGDMLEMGEHSAAVHAALARPIVDAGIADVWLTGTEMAHLRDALPSE
CCCCCCCCCEEEEEHHHHHCCCHHHHHHHHHHHHHHHCCCHHEEECCCHHHHHHHCCCHH
VSVVHRDLVSDLTAYALGAVAAGDVVMVKSSKSTGCAKIVQALLDAHPHDEAGKREA
HHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MNWLWTSSDLLGAMNGRPVGNLPEGITGISIDSRTIGNGEAFFAIRGDRVDGHDYAGIAL
CCCEECCHHHHHCCCCCCCCCCCCCCEEEEECCEECCCCCEEEEEECCCCCCCCCCCEEE
ANGAALLVVSEAKLPALGRLIAPMIVVDDVLEAMIRLGCAARDRSAAKIIAVTGSVGKTT
ECCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCHH
TKEMLRHMLSPLGRVHASVASFNNHWGVPLTLARMPETTDYGVFEIGMNHAGEIRPLTRM
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCEEEECCCCCCCHHHHHHH
VRPHVAVVTSIAAAHLGNFASLDDIAAAKAEIFEGVVNGGHAVINRDSAQYELLEKAAAT
HCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHH
AGVSHVHFFGANPKSEFRLAEFAGGAEGSVLWAGVGGRTLEIAIGAPGRHIAENALAAIA
CCCCEEEEECCCCCCCEEHHHHCCCCCCCEEEEECCCEEEEEEECCCCHHHHHHHHHHHH
AATLAGADTDRVAASLATMQPEKGRGLRHRLRMGAGYMTLIDESYNANPASMRAAITLLR
HHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHH
DAEPSPGGRRIAILGDMLEMGEHSAAVHAALARPIVDAGIADVWLTGTEMAHLRDALPSE
CCCCCCCCCEEEEEHHHHHCCCHHHHHHHHHHHHHHHCCCHHEEECCCHHHHHHHCCCHH
VSVVHRDLVSDLTAYALGAVAAGDVVMVKSSKSTGCAKIVQALLDAHPHDEAGKREA
HHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]