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The map label for this gene is cobA [H]

Identifier: 150396949

GI number: 150396949

Start: 1826125

End: 1826964

Strand: Reverse

Name: cobA [H]

Synonym: Smed_1746

Alternate gene names: 150396949

Gene position: 1826964-1826125 (Counterclockwise)

Preceding gene: 150396950

Following gene: 150396948

Centisome position: 48.31

GC content: 62.62

Gene sequence:

>840_bases
ATGACGGATATCTTTACCGGCTTGCCTGAACTCGAGGCTGGTTCGGTCTGGCTCGTCGGAGCGGGTCCGGGCGACCCCGG
CCTTTTGACGTTGCATGCCGCGAATGCGCTCCGTCAGGCCGATGTGATCGTTCACGATGCCCTGGTCAACGCGGATTGTC
TTGAGTTGGCCAAGCCGAGCGCAACTCTCGAATTTGCGGGCAAGCGCGGCGGCAAGCCTTCGCCGAAGCAACGCGACATC
TCGCTGCGTCTCGTCGAACTGGCACGCGAGGGGAAAAGAGTCTTGCGTCTCAAGGGCGGTGATCCTTTCGTCTTCGGGCG
AGGCGGAGAAGAGGCACTGACCCTGGTCGAGCACCGCATACCGTTCCGCATCGTTCCCGGCATCACAGCCGGGATAGGCG
GGCTTGCCTATGCCGGTATTCCGGTAACCCATCGCGAGGTCAATCATGCTGTCACATTCCTGACGGGCCATGATTCTTCG
GGTGTCGTCCCGGACAGGATCGACTGGGTCGGCATTGCCAGGGGCTCTCCAGTCATCGTCATGTACATGGCGATGAAACA
TATCGGCCAGATCTCCGCCAACCTCATCGCCGCCGGTCGCTCGCCGGATGAGCCGGTCGCCTTTGTCTGCAACGCCGCGA
CACCGCAGCAGGTGGTGCTTGAAACAACGCTTTGCCGCTGCGAAACCGACATCGCCGCTTCGGGACTCGAGCCGCCGGCA
ATCGTCGTTGTCGGCGAGGTCGTGCGGCTGCGCGCCTCACTCGATTGGCTCGGTGCGCTCGGAGGCCGAGCGCTTGCTCC
CGATCCGTTTTCGAGCCGCATTCTCCGGAATCCGGCATGA

Upstream 100 bases:

>100_bases
AGCCGATTCAATTGTCTGTGGCAGTTTCCTTCATACTGACTTTAAGTTCTTGCTCGGTCCGACCATATGGAATGCCTTGC
TTTGCCCGGAGTGTCGACCG

Downstream 100 bases:

>100_bases
AGGGGATCATGATTGCAGCCCCGAGTTCCGGCTCTGGCAAAACCACCGTGACCCTCGGCCTGATGCGGGCGCTGAAGCGG
CGCGGAGTATCGATCGCCCC

Product: uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM [H]

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MTDIFTGLPELEAGSVWLVGAGPGDPGLLTLHAANALRQADVIVHDALVNADCLELAKPSATLEFAGKRGGKPSPKQRDI
SLRLVELAREGKRVLRLKGGDPFVFGRGGEEALTLVEHRIPFRIVPGITAGIGGLAYAGIPVTHREVNHAVTFLTGHDSS
GVVPDRIDWVGIARGSPVIVMYMAMKHIGQISANLIAAGRSPDEPVAFVCNAATPQQVVLETTLCRCETDIAASGLEPPA
IVVVGEVVRLRASLDWLGALGGRALAPDPFSSRILRNPA

Sequences:

>Translated_279_residues
MTDIFTGLPELEAGSVWLVGAGPGDPGLLTLHAANALRQADVIVHDALVNADCLELAKPSATLEFAGKRGGKPSPKQRDI
SLRLVELAREGKRVLRLKGGDPFVFGRGGEEALTLVEHRIPFRIVPGITAGIGGLAYAGIPVTHREVNHAVTFLTGHDSS
GVVPDRIDWVGIARGSPVIVMYMAMKHIGQISANLIAAGRSPDEPVAFVCNAATPQQVVLETTLCRCETDIAASGLEPPA
IVVVGEVVRLRASLDWLGALGGRALAPDPFSSRILRNPA
>Mature_278_residues
TDIFTGLPELEAGSVWLVGAGPGDPGLLTLHAANALRQADVIVHDALVNADCLELAKPSATLEFAGKRGGKPSPKQRDIS
LRLVELAREGKRVLRLKGGDPFVFGRGGEEALTLVEHRIPFRIVPGITAGIGGLAYAGIPVTHREVNHAVTFLTGHDSSG
VVPDRIDWVGIARGSPVIVMYMAMKHIGQISANLIAAGRSPDEPVAFVCNAATPQQVVLETTLCRCETDIAASGLEPPAI
VVVGEVVRLRASLDWLGALGGRALAPDPFSSRILRNPA

Specific function: Catalyzes the methylation of both C-2 and C-7 of uroporphyrinogen III leading to precorrin-1 and precorrin-2; their oxidative esterification gives respectively factor I octamethyl ester and sirohydrochlorin [H]

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=244, Percent_Identity=43.8524590163934, Blast_Score=195, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6322922, Length=255, Percent_Identity=30.9803921568627, Blast_Score=114, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.107 [H]

Molecular weight: Translated: 29370; Mature: 29239

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDIFTGLPELEAGSVWLVGAGPGDPGLLTLHAANALRQADVIVHDALVNADCLELAKPS
CCCCCCCCCCCCCCCEEEEECCCCCCCEEEEECHHHHHHHHHEEHHHHCCCHHHHHCCCC
ATLEFAGKRGGKPSPKQRDISLRLVELAREGKRVLRLKGGDPFVFGRGGEEALTLVEHRI
CEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCEEEEECCCHHHHHHHHHCC
PFRIVPGITAGIGGLAYAGIPVTHREVNHAVTFLTGHDSSGVVPDRIDWVGIARGSPVIV
CEEECCCCHHCCCCHHHCCCCCCHHCCCEEEEEEECCCCCCCCCCCCCEEEECCCCCEEE
MYMAMKHIGQISANLIAAGRSPDEPVAFVCNAATPQQVVLETTLCRCETDIAASGLEPPA
HHHHHHHHHHHHHEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCHHHCCCCCCE
IVVVGEVVRLRASLDWLGALGGRALAPDPFSSRILRNPA
EEEEHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCC
>Mature Secondary Structure 
TDIFTGLPELEAGSVWLVGAGPGDPGLLTLHAANALRQADVIVHDALVNADCLELAKPS
CCCCCCCCCCCCCCEEEEECCCCCCCEEEEECHHHHHHHHHEEHHHHCCCHHHHHCCCC
ATLEFAGKRGGKPSPKQRDISLRLVELAREGKRVLRLKGGDPFVFGRGGEEALTLVEHRI
CEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCEEEEECCCHHHHHHHHHCC
PFRIVPGITAGIGGLAYAGIPVTHREVNHAVTFLTGHDSSGVVPDRIDWVGIARGSPVIV
CEEECCCCHHCCCCHHHCCCCCCHHCCCEEEEEEECCCCCCCCCCCCCEEEECCCCCEEE
MYMAMKHIGQISANLIAAGRSPDEPVAFVCNAATPQQVVLETTLCRCETDIAASGLEPPA
HHHHHHHHHHHHHEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCHHHCCCCCCE
IVVVGEVVRLRASLDWLGALGGRALAPDPFSSRILRNPA
EEEEHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2211520; 2546914 [H]