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The map label for this gene is pcm [H]

Identifier: 150396388

GI number: 150396388

Start: 1245925

End: 1246578

Strand: Direct

Name: pcm [H]

Synonym: Smed_1169

Alternate gene names: 150396388

Gene position: 1245925-1246578 (Clockwise)

Preceding gene: 150396387

Following gene: 150396389

Centisome position: 32.94

GC content: 61.77

Gene sequence:

>654_bases
TTGAACGCACGGGTTTCCCAGCAGGAGGGGTTTGCAGCGATGGTGCTGCGGCTTCGCTCGGGCGGCATCGTCAACCATGA
GCTTCTGAAGGCCGTGGAGCAGACGCCCCGGCTGCTTTTCGCTCCGCCGCAATATCAGGACGAAGTCTACTCCAAGCGGC
TGATCCCGCTCGATTGCGGTTCCTTCATGGAAGGCTGCGACATGGCCGTCCGGCTTTTGCACTGTCTCAACCTGAAGTCC
GGCCAGCGTATACTCGAGGTCGGAACCGGCAGCGGCTTTACGGCGGCCGTTATGGGGCGGATCGCCGAGCGCGTGCTGAC
GATCGACCGATATCAGACGCTGGTCGCATCGGCGCAAAAGAACCTGGAGAAGGCGGGCATCCGCAACGTCGTCGTCCGGC
AGGCGGATGGTAGCGCAGGCGTTCCGGGCGAGGGCACGTTCGACCGCATCCTCATTACCGCTGCGTTCAACAGCCTTCCG
CGGATGTTCTCTGATCACCTCGTTTCCGGTGGTACCCTGCTTGTGCCGATCATGATGAGCGAAACGCATTGCCGGATCGT
TCGCGTCAACCGCACCGGCAGCCGTTTCGACCGGGAAGATCTGTTCGATGCGCCTTACCTTCCGATCGTCCCCCAGGTAG
CGTCGTTCCTTTAG

Upstream 100 bases:

>100_bases
TGCGGGATAACAAGATATCGGTAACCCCGCTTAAACTAGATATGACCGATCATGCCGTTCAGGATCTTATAGCGCGCGCA
TTGCGGAAAGGGACGGTCGC

Downstream 100 bases:

>100_bases
AGCGGCTCGGCGTGTTCAGAACAAGCGCACCATACGATCTTAATAGAATCTTGACTCGCTCCAGCTCCGCGCAAGCTTGC
AGTCCTAGGCCGGACAGCAT

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT [H]

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MNARVSQQEGFAAMVLRLRSGGIVNHELLKAVEQTPRLLFAPPQYQDEVYSKRLIPLDCGSFMEGCDMAVRLLHCLNLKS
GQRILEVGTGSGFTAAVMGRIAERVLTIDRYQTLVASAQKNLEKAGIRNVVVRQADGSAGVPGEGTFDRILITAAFNSLP
RMFSDHLVSGGTLLVPIMMSETHCRIVRVNRTGSRFDREDLFDAPYLPIVPQVASFL

Sequences:

>Translated_217_residues
MNARVSQQEGFAAMVLRLRSGGIVNHELLKAVEQTPRLLFAPPQYQDEVYSKRLIPLDCGSFMEGCDMAVRLLHCLNLKS
GQRILEVGTGSGFTAAVMGRIAERVLTIDRYQTLVASAQKNLEKAGIRNVVVRQADGSAGVPGEGTFDRILITAAFNSLP
RMFSDHLVSGGTLLVPIMMSETHCRIVRVNRTGSRFDREDLFDAPYLPIVPQVASFL
>Mature_217_residues
MNARVSQQEGFAAMVLRLRSGGIVNHELLKAVEQTPRLLFAPPQYQDEVYSKRLIPLDCGSFMEGCDMAVRLLHCLNLKS
GQRILEVGTGSGFTAAVMGRIAERVLTIDRYQTLVASAQKNLEKAGIRNVVVRQADGSAGVPGEGTFDRILITAAFNSLP
RMFSDHLVSGGTLLVPIMMSETHCRIVRVNRTGSRFDREDLFDAPYLPIVPQVASFL

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789100, Length=198, Percent_Identity=28.7878787878788, Blast_Score=105, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 23864; Mature: 23864

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: PS01279 PCMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNARVSQQEGFAAMVLRLRSGGIVNHELLKAVEQTPRLLFAPPQYQDEVYSKRLIPLDCG
CCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCEEECCCCHHHHHHHCCCCCCCCH
SFMEGCDMAVRLLHCLNLKSGQRILEVGTGSGFTAAVMGRIAERVLTIDRYQTLVASAQK
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLEKAGIRNVVVRQADGSAGVPGEGTFDRILITAAFNSLPRMFSDHLVSGGTLLVPIMMS
HHHHHCHHHHEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEC
ETHCRIVRVNRTGSRFDREDLFDAPYLPIVPQVASFL
CCCEEEEEECCCCCCCCHHHHCCCCCCCHHHHHHHCC
>Mature Secondary Structure
MNARVSQQEGFAAMVLRLRSGGIVNHELLKAVEQTPRLLFAPPQYQDEVYSKRLIPLDCG
CCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCEEECCCCHHHHHHHCCCCCCCCH
SFMEGCDMAVRLLHCLNLKSGQRILEVGTGSGFTAAVMGRIAERVLTIDRYQTLVASAQK
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLEKAGIRNVVVRQADGSAGVPGEGTFDRILITAAFNSLPRMFSDHLVSGGTLLVPIMMS
HHHHHCHHHHEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEC
ETHCRIVRVNRTGSRFDREDLFDAPYLPIVPQVASFL
CCCEEEEEECCCCCCCCHHHHCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9304864; 11481430 [H]