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The map label for this gene is ntrB [H]

Identifier: 150396308

GI number: 150396308

Start: 1156651

End: 1157793

Strand: Direct

Name: ntrB [H]

Synonym: Smed_1089

Alternate gene names: 150396308

Gene position: 1156651-1157793 (Clockwise)

Preceding gene: 150396307

Following gene: 150396309

Centisome position: 30.58

GC content: 61.07

Gene sequence:

>1143_bases
ATGACAGAGAAGGCACCGGCTGACGGCGCAAACGATCTTTCCATGGCCGTGCTGAACGCGATTCAGAATCCGGTCATCCT
GGTGGATGAGAACGGCTTTGTCGCCTTTGCGAACTGGGAGGCGGAATCCTTCTTCGGTGCCAGCGCCAACCATCTTGCGC
GGCATGACATCAGCGCGTTCATTCCCTTCGGCAGCCCGCTCCTGACGCTGATCGATCAGGTTCGCGAGCGGCGCGCTGCG
GTGAACGAGTACCGGGTAGACCTCAGCTCTCCGCGCCTCGGGGCCGACAAGCTCGTCGATCTCTACGTGGCCCCGGTACT
CTCGCAACCCGGATCGGTGGTGGTCGTCTTTCAAGAGCGGTCGATGGCGGACAAGATCGATCGCCAGCTGACACATCGGA
CGGCGGCGCGATCCGTCACGGGGCTTGCTTCGATGCTGGCGCACGAAATCAAAAACCCTCTTTCCGGCATTCGCGGCGCC
GCGCAGCTCCTCGAAACCTCCGTCAACGACGAAGACAGGGCGCTCACAAGATTGATCTGTGACGAGACCGACCGCATCGT
CTCGCTCGTGGACCGGATGGAGGTCTTCTCCGACGAGCGTCCCGTCGACCGCGTGCCCCTTAACATTCATGCGATACTCG
ACCACGTCAAGGCAATCGCGAAGGCCGGCTTTGCCCGGCGGATCAAGATCTCCGAACATTATGACCCGTCGCTTCCGCCC
GTTTTCGCAAATCGCGACCAGCTGGTTCAGGTGTTCCTCAATCTGGTAAAGAATGCGGCCGAGGCGATCGGCGACAGGGC
GGACGGCGAAATTCTGCTGACGACGGCCTATAGGCCGGGCATTCGCCTCTCGGTCGCCGGTACGCGCGAAAAAATCTCGC
TCCCGCTGGAATTCTGCGTGCATGACAACGGGCCGGGTGTACCCGCCGATCTTCTGCCGCATCTTTTCGACCCTTTCATC
ACCACCAAGACGAACGGGTCCGGCCTCGGTCTCGCGCTTGTGGCGAAGATCATCGGCGGCCATGGCGGCATCGTCGAATG
CGACAGCCAGCATAGCCGCACGATATTCCGCGTTCTGATGCCGGCGTCCAAGGGCCCAGCGGCAGATGACGAAACTCCGA
TGACAAAAGGAACCAATGGATGA

Upstream 100 bases:

>100_bases
CTGCGATCCTCACATCGACGGACACGGTTTTCGTGCGCGACGGCGTTGCGGCCGCCATTGCCCGCTCCGGCGATGTGGCG
GCGAGGGAGGAGATTGCGGC

Downstream 100 bases:

>100_bases
CGGGTGCAACGATCCTTGTCGCGGATGACGATGCAGCCATTCGCACCGTTCTGAATCAGGCGCTCAGCCGTGCCGGATAC
GATGTGCGCATCACCTCCAA

Product: nitrogen-specific signal transduction histidine kinase NtrB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MTEKAPADGANDLSMAVLNAIQNPVILVDENGFVAFANWEAESFFGASANHLARHDISAFIPFGSPLLTLIDQVRERRAA
VNEYRVDLSSPRLGADKLVDLYVAPVLSQPGSVVVVFQERSMADKIDRQLTHRTAARSVTGLASMLAHEIKNPLSGIRGA
AQLLETSVNDEDRALTRLICDETDRIVSLVDRMEVFSDERPVDRVPLNIHAILDHVKAIAKAGFARRIKISEHYDPSLPP
VFANRDQLVQVFLNLVKNAAEAIGDRADGEILLTTAYRPGIRLSVAGTREKISLPLEFCVHDNGPGVPADLLPHLFDPFI
TTKTNGSGLGLALVAKIIGGHGGIVECDSQHSRTIFRVLMPASKGPAADDETPMTKGTNG

Sequences:

>Translated_380_residues
MTEKAPADGANDLSMAVLNAIQNPVILVDENGFVAFANWEAESFFGASANHLARHDISAFIPFGSPLLTLIDQVRERRAA
VNEYRVDLSSPRLGADKLVDLYVAPVLSQPGSVVVVFQERSMADKIDRQLTHRTAARSVTGLASMLAHEIKNPLSGIRGA
AQLLETSVNDEDRALTRLICDETDRIVSLVDRMEVFSDERPVDRVPLNIHAILDHVKAIAKAGFARRIKISEHYDPSLPP
VFANRDQLVQVFLNLVKNAAEAIGDRADGEILLTTAYRPGIRLSVAGTREKISLPLEFCVHDNGPGVPADLLPHLFDPFI
TTKTNGSGLGLALVAKIIGGHGGIVECDSQHSRTIFRVLMPASKGPAADDETPMTKGTNG
>Mature_379_residues
TEKAPADGANDLSMAVLNAIQNPVILVDENGFVAFANWEAESFFGASANHLARHDISAFIPFGSPLLTLIDQVRERRAAV
NEYRVDLSSPRLGADKLVDLYVAPVLSQPGSVVVVFQERSMADKIDRQLTHRTAARSVTGLASMLAHEIKNPLSGIRGAA
QLLETSVNDEDRALTRLICDETDRIVSLVDRMEVFSDERPVDRVPLNIHAILDHVKAIAKAGFARRIKISEHYDPSLPPV
FANRDQLVQVFLNLVKNAAEAIGDRADGEILLTTAYRPGIRLSVAGTREKISLPLEFCVHDNGPGVPADLLPHLFDPFIT
TKTNGSGLGLALVAKIIGGHGGIVECDSQHSRTIFRVLMPASKGPAADDETPMTKGTNG

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=372, Percent_Identity=31.1827956989247, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1788549, Length=335, Percent_Identity=28.0597014925373, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1790436, Length=224, Percent_Identity=32.1428571428571, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1788393, Length=215, Percent_Identity=29.3023255813954, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1789808, Length=217, Percent_Identity=25.8064516129032, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1790567, Length=103, Percent_Identity=34.9514563106796, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 41128; Mature: 40997

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEKAPADGANDLSMAVLNAIQNPVILVDENGFVAFANWEAESFFGASANHLARHDISAF
CCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCEEEEECCCHHHHCCCCHHHHHHHCCCEE
IPFGSPLLTLIDQVRERRAAVNEYRVDLSSPRLGADKLVDLYVAPVLSQPGSVVVVFQER
CCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCC
SMADKIDRQLTHRTAARSVTGLASMLAHEIKNPLSGIRGAAQLLETSVNDEDRALTRLIC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
DETDRIVSLVDRMEVFSDERPVDRVPLNIHAILDHVKAIAKAGFARRIKISEHYDPSLPP
CCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCC
VFANRDQLVQVFLNLVKNAAEAIGDRADGEILLTTAYRPGIRLSVAGTREKISLPLEFCV
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEECCCCCCEECCEEEEE
HDNGPGVPADLLPHLFDPFITTKTNGSGLGLALVAKIIGGHGGIVECDSQHSRTIFRVLM
ECCCCCCCHHHHHHHHCCCEEECCCCCCHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHC
PASKGPAADDETPMTKGTNG
CCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TEKAPADGANDLSMAVLNAIQNPVILVDENGFVAFANWEAESFFGASANHLARHDISAF
CCCCCCCCCCHHHHHHHHHHCCCEEEEECCCEEEEECCCHHHHCCCCHHHHHHHCCCEE
IPFGSPLLTLIDQVRERRAAVNEYRVDLSSPRLGADKLVDLYVAPVLSQPGSVVVVFQER
CCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCC
SMADKIDRQLTHRTAARSVTGLASMLAHEIKNPLSGIRGAAQLLETSVNDEDRALTRLIC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
DETDRIVSLVDRMEVFSDERPVDRVPLNIHAILDHVKAIAKAGFARRIKISEHYDPSLPP
CCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCC
VFANRDQLVQVFLNLVKNAAEAIGDRADGEILLTTAYRPGIRLSVAGTREKISLPLEFCV
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEECCCCCCEECCEEEEE
HDNGPGVPADLLPHLFDPFITTKTNGSGLGLALVAKIIGGHGGIVECDSQHSRTIFRVLM
ECCCCCCCHHHHHHHHCCCEEECCCCCCHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHC
PASKGPAADDETPMTKGTNG
CCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430; 2881918 [H]