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The map label for this gene is ntrC [H]

Identifier: 150396309

GI number: 150396309

Start: 1157790

End: 1159244

Strand: Direct

Name: ntrC [H]

Synonym: Smed_1090

Alternate gene names: 150396309

Gene position: 1157790-1159244 (Clockwise)

Preceding gene: 150396308

Following gene: 150396310

Centisome position: 30.61

GC content: 60.62

Gene sequence:

>1455_bases
ATGACGGGTGCAACGATCCTTGTCGCGGATGACGATGCAGCCATTCGCACCGTTCTGAATCAGGCGCTCAGCCGTGCCGG
ATACGATGTGCGCATCACCTCCAATGCCGCCACACTATGGCGCTGGATAGCCGCCGGCGACGGCGATCTGGTCGTGACCG
ATGTCGTGATGCCGGATGAGAACGCCTTCGATCTCCTGCCGCGGATCAAGAAAGCTCGGCCGGATCTGCCGGTGCTTGTG
ATGAGCGCTCAAAACACCTTCATGACGGCCATCAAGGCTTCGGAAAAGGGCGCCTATGACTATCTGCCGAAGCCGTTCGA
TCTGACGGAGCTGATCGGCATTATCGGCCGGGCGCTCGCCGAACCGAAGCGGCGGCCGTCGAAACTCGACGACGATTCCC
AGGACGGCATGCCTCTCGTCGGACGCTCCGCCGCGATGCAGGAAATCTACCGCGTGCTTGCCCGGTTGATGCAGACGGAC
CTGACGCTGATGATCACCGGCGAGTCGGGAACCGGCAAGGAACTGGTTGCGCGGGCATTGCACGATTATGGCAAGCGGAG
AAACGGTCCCTTCGTCGCCATCAATATGGCGGCGATTCCGCGCGATCTCATCGAGTCGGAACTGTTCGGTCACGAAAAGG
GGGCTTTCACCGGCGCTCAAACCCGCTCCACCGGTCGTTTCGAGCAGGCCGAAGGGGGAACGCTCTTCCTCGATGAGATC
GGCGACATGCCCATGGATGCGCAGACGCGTCTCCTGCGTGTGCTGCAGCAGGGCGAATACACGACCGTCGGCGGACGCAC
GCCGATTAGATCGGACGTCCGCATCGTCGCGGCAACCAACAAGGACCTGAAACAATCGATCAATCAGGGTCTTTTCCGCG
AGGACCTCTACTATCGTCTGAACGTCGTGCCGCTTCGCCTGCCGCCGCTTCGGGACCGGGCCGAGGATATTCCGGATCTC
GTCCGCCACTTCGTCCAGCAAGCCGAAAAAGAGGGCCTGGACGTCAAGCGCTTCGATCAGGAGGCGCTGGAGCTGATGAA
AGCACATCCCTGGCCGGGCAATGTGCGCGAACTCGAAAACCTCATTCGCCGTTTGACGGCACTTTATCCACAGGACGTCA
TCACGCGCGAAATCATCGAAAACGAGCTGCGTTCGGAGATCCCGGACAGTCCGATCGAGAAAACCGCAGCCCGCTCCGGC
TCGCTGTCGATCTCGCAGGCCGTGGAGGAGAACATGAGGCAGTATTTCGCGAGCTTTGGCGATGGTTTACCGCCCTCTGG
TCTTTATGACCGCGTCCTCGCGGAGATGGAATATCCTCTGATTCTCGCGGCCTTGACGGCTACCCGCGGCAACCAGATCA
AGGCGGCCGACCTGCTGGGTCTCAACCGCAATACGCTGCGCAAGAAGATCCGTGAACTCGGTGTCTCTGTCTATCGCAGC
TCGCGTAGCGCTTGA

Upstream 100 bases:

>100_bases
GCGACAGCCAGCATAGCCGCACGATATTCCGCGTTCTGATGCCGGCGTCCAAGGGCCCAGCGGCAGATGACGAAACTCCG
ATGACAAAAGGAACCAATGG

Downstream 100 bases:

>100_bases
CTGAGATGCAACACCGTTGCATTTTCGCCACAATGCGTTGCTTGAACCGCGCATAGCGCCGATTCGCCGGCGGTCGGTCG
CAGCCGATCGTGCGGAAACC

Product: Fis family nitrogen metabolism transcriptional regulator NtrC

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MTGATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFDLLPRIKKARPDLPVLV
MSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKRRPSKLDDDSQDGMPLVGRSAAMQEIYRVLARLMQTD
LTLMITGESGTGKELVARALHDYGKRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQTRSTGRFEQAEGGTLFLDEI
GDMPMDAQTRLLRVLQQGEYTTVGGRTPIRSDVRIVAATNKDLKQSINQGLFREDLYYRLNVVPLRLPPLRDRAEDIPDL
VRHFVQQAEKEGLDVKRFDQEALELMKAHPWPGNVRELENLIRRLTALYPQDVITREIIENELRSEIPDSPIEKTAARSG
SLSISQAVEENMRQYFASFGDGLPPSGLYDRVLAEMEYPLILAALTATRGNQIKAADLLGLNRNTLRKKIRELGVSVYRS
SRSA

Sequences:

>Translated_484_residues
MTGATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFDLLPRIKKARPDLPVLV
MSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKRRPSKLDDDSQDGMPLVGRSAAMQEIYRVLARLMQTD
LTLMITGESGTGKELVARALHDYGKRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQTRSTGRFEQAEGGTLFLDEI
GDMPMDAQTRLLRVLQQGEYTTVGGRTPIRSDVRIVAATNKDLKQSINQGLFREDLYYRLNVVPLRLPPLRDRAEDIPDL
VRHFVQQAEKEGLDVKRFDQEALELMKAHPWPGNVRELENLIRRLTALYPQDVITREIIENELRSEIPDSPIEKTAARSG
SLSISQAVEENMRQYFASFGDGLPPSGLYDRVLAEMEYPLILAALTATRGNQIKAADLLGLNRNTLRKKIRELGVSVYRS
SRSA
>Mature_483_residues
TGATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFDLLPRIKKARPDLPVLVM
SAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKRRPSKLDDDSQDGMPLVGRSAAMQEIYRVLARLMQTDL
TLMITGESGTGKELVARALHDYGKRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQTRSTGRFEQAEGGTLFLDEIG
DMPMDAQTRLLRVLQQGEYTTVGGRTPIRSDVRIVAATNKDLKQSINQGLFREDLYYRLNVVPLRLPPLRDRAEDIPDLV
RHFVQQAEKEGLDVKRFDQEALELMKAHPWPGNVRELENLIRRLTALYPQDVITREIIENELRSEIPDSPIEKTAARSGS
LSISQAVEENMRQYFASFGDGLPPSGLYDRVLAEMEYPLILAALTATRGNQIKAADLLGLNRNTLRKKIRELGVSVYRSS
RSA

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=484, Percent_Identity=44.8347107438017, Blast_Score=405, Evalue=1e-114,
Organism=Escherichia coli, GI1788550, Length=477, Percent_Identity=38.3647798742138, Blast_Score=327, Evalue=1e-90,
Organism=Escherichia coli, GI1790437, Length=466, Percent_Identity=41.2017167381974, Blast_Score=319, Evalue=2e-88,
Organism=Escherichia coli, GI1788905, Length=384, Percent_Identity=40.1041666666667, Blast_Score=280, Evalue=1e-76,
Organism=Escherichia coli, GI87082117, Length=343, Percent_Identity=43.4402332361516, Blast_Score=247, Evalue=1e-66,
Organism=Escherichia coli, GI1789087, Length=348, Percent_Identity=41.3793103448276, Blast_Score=246, Evalue=3e-66,
Organism=Escherichia coli, GI1789233, Length=337, Percent_Identity=42.7299703264095, Blast_Score=244, Evalue=1e-65,
Organism=Escherichia coli, GI87082152, Length=231, Percent_Identity=45.8874458874459, Blast_Score=214, Evalue=7e-57,
Organism=Escherichia coli, GI87081872, Length=344, Percent_Identity=37.7906976744186, Blast_Score=208, Evalue=5e-55,
Organism=Escherichia coli, GI1786524, Length=249, Percent_Identity=43.3734939759036, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI1787583, Length=243, Percent_Identity=41.9753086419753, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1789828, Length=231, Percent_Identity=37.2294372294372, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI87081858, Length=323, Percent_Identity=29.1021671826625, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1786784, Length=107, Percent_Identity=31.7757009345794, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1789809, Length=110, Percent_Identity=32.7272727272727, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1790552, Length=106, Percent_Identity=36.7924528301887, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1789661, Length=70, Percent_Identity=42.8571428571429, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 53819; Mature: 53688

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDE
CCCCEEEEECCHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCEEEEEEECCCC
NAFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALA
CHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
EPKRRPSKLDDDSQDGMPLVGRSAAMQEIYRVLARLMQTDLTLMITGESGTGKELVARAL
CHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHH
HDYGKRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQTRSTGRFEQAEGGTLFLDEI
HHHHHCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHH
GDMPMDAQTRLLRVLQQGEYTTVGGRTPIRSDVRIVAATNKDLKQSINQGLFREDLYYRL
CCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHEEE
NVVPLRLPPLRDRAEDIPDLVRHFVQQAEKEGLDVKRFDQEALELMKAHPWPGNVRELEN
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHH
LIRRLTALYPQDVITREIIENELRSEIPDSPIEKTAARSGSLSISQAVEENMRQYFASFG
HHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHC
DGLPPSGLYDRVLAEMEYPLILAALTATRGNQIKAADLLGLNRNTLRKKIRELGVSVYRS
CCCCCCHHHHHHHHHCCCCHHHHHHHHCCCCCEEHHHHHCCCHHHHHHHHHHHHHHHHHH
SRSA
CCCC
>Mature Secondary Structure 
TGATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDE
CCCEEEEECCHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCEEEEEEECCCC
NAFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALA
CHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
EPKRRPSKLDDDSQDGMPLVGRSAAMQEIYRVLARLMQTDLTLMITGESGTGKELVARAL
CHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHH
HDYGKRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQTRSTGRFEQAEGGTLFLDEI
HHHHHCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHH
GDMPMDAQTRLLRVLQQGEYTTVGGRTPIRSDVRIVAATNKDLKQSINQGLFREDLYYRL
CCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHEEE
NVVPLRLPPLRDRAEDIPDLVRHFVQQAEKEGLDVKRFDQEALELMKAHPWPGNVRELEN
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHH
LIRRLTALYPQDVITREIIENELRSEIPDSPIEKTAARSGSLSISQAVEENMRQYFASFG
HHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHC
DGLPPSGLYDRVLAEMEYPLILAALTATRGNQIKAADLLGLNRNTLRKKIRELGVSVYRS
CCCCCCHHHHHHHHHCCCCHHHHHHHHCCCCCEEHHHHHCCCHHHHHHHHHHHHHHHHHH
SRSA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2881918; 11481430; 8478331 [H]