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The map label for this gene is dus [H]

Identifier: 150396307

GI number: 150396307

Start: 1155641

End: 1156654

Strand: Direct

Name: dus [H]

Synonym: Smed_1088

Alternate gene names: 150396307

Gene position: 1155641-1156654 (Clockwise)

Preceding gene: 150396304

Following gene: 150396308

Centisome position: 30.56

GC content: 62.33

Gene sequence:

>1014_bases
ATGCATTTGCCATCCACGGCTCTTTCATCTTCGTTTCTGATCGGGAATGTCCAAATCCGTAATCGCGTAGCGCTTGCGCC
CATGTCCGGCGTGACGGATCTGCCCTTTCGCATGCTTGCCTGGCGCTTCGGCGCGGGCTTTGTCGTGACCGAGATGGTGG
CGAGTCGCGAGCTGGTTGGCAATGCCGCCGAATCCTGGGCGCGGCTGAAGAATTCCGGCATCGATCCGCATATTGTCCAA
CTTGCCGGGCGTGAGGCGCATTGGATGGCCGAGGCGGCGAGGATCGTTGAGGCCAATGGTGCCGATATCATCGATATCAA
CATGGGCTGTCCCGCCAAGAAGGTTACCGGTGGCTATTCCGGATCTGCACTCATGCGCGACCCGGATCACGCGCTGTCTC
TGATCGAGGCGACGGTGAAAGCAGCGACGGTTCCGGTAACGCTCAAGATGCGACTCGGTTGGGATGAGAATTCGATCAAT
GCGCCGCTGATTGCCCGCAGAGCCGAGGAAGCCGGCGTGAAGGCGATCACCATTCACGGGCGCACGCGCATGCAGTTCTA
CAACGGCAAGGCCGATTGGGATGCGATCCGCTCGGTCCGCGAGGTGACTTCGGTCCCGCTGATCGCCAATGGCGATGTTG
ATTCCGTTGCGGATGCGCATGAAATCCTGCGCCGCTCGGGTGCCGACGCCGTCATGGTCGGTCGATCTTGCCAGGGTCGG
CCGTGGCATGCGGGCGTGCTCGCCGGCACGGCTGCCCATCCGGATGCGGCGGGCGTCGCACGCATATTTGCGGAGCATTA
CGAGACGCTCCTTGAATTCTACGGCGTGGAGGTCGGCCTTCGCACTGCCCGAAAGCATGCCGGCTGGTATCTCGATCGTT
TCGCAACCGAGCTCCCCGTCTCGCAGAAGGCTGCGATCCTCACATCGACGGACACGGTTTTCGTGCGCGACGGCGTTGCG
GCCGCCATTGCCCGCTCCGGCGATGTGGCGGCGAGGGAGGAGATTGCGGCATGA

Upstream 100 bases:

>100_bases
CGACGCGCCAAAAGGAATTTTGTCCCGCATGCCGAAAAAGCTTGGCAAGCGGAAGAACAATGTCTAAAAATAGTGCACGA
TTTCAAGGTGCCTGAAAGAT

Downstream 100 bases:

>100_bases
CAGAGAAGGCACCGGCTGACGGCGCAAACGATCTTTCCATGGCCGTGCTGAACGCGATTCAGAATCCGGTCATCCTGGTG
GATGAGAACGGCTTTGTCGC

Product: nifR3 family TIM-barrel protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MHLPSTALSSSFLIGNVQIRNRVALAPMSGVTDLPFRMLAWRFGAGFVVTEMVASRELVGNAAESWARLKNSGIDPHIVQ
LAGREAHWMAEAARIVEANGADIIDINMGCPAKKVTGGYSGSALMRDPDHALSLIEATVKAATVPVTLKMRLGWDENSIN
APLIARRAEEAGVKAITIHGRTRMQFYNGKADWDAIRSVREVTSVPLIANGDVDSVADAHEILRRSGADAVMVGRSCQGR
PWHAGVLAGTAAHPDAAGVARIFAEHYETLLEFYGVEVGLRTARKHAGWYLDRFATELPVSQKAAILTSTDTVFVRDGVA
AAIARSGDVAAREEIAA

Sequences:

>Translated_337_residues
MHLPSTALSSSFLIGNVQIRNRVALAPMSGVTDLPFRMLAWRFGAGFVVTEMVASRELVGNAAESWARLKNSGIDPHIVQ
LAGREAHWMAEAARIVEANGADIIDINMGCPAKKVTGGYSGSALMRDPDHALSLIEATVKAATVPVTLKMRLGWDENSIN
APLIARRAEEAGVKAITIHGRTRMQFYNGKADWDAIRSVREVTSVPLIANGDVDSVADAHEILRRSGADAVMVGRSCQGR
PWHAGVLAGTAAHPDAAGVARIFAEHYETLLEFYGVEVGLRTARKHAGWYLDRFATELPVSQKAAILTSTDTVFVRDGVA
AAIARSGDVAAREEIAA
>Mature_337_residues
MHLPSTALSSSFLIGNVQIRNRVALAPMSGVTDLPFRMLAWRFGAGFVVTEMVASRELVGNAAESWARLKNSGIDPHIVQ
LAGREAHWMAEAARIVEANGADIIDINMGCPAKKVTGGYSGSALMRDPDHALSLIEATVKAATVPVTLKMRLGWDENSIN
APLIARRAEEAGVKAITIHGRTRMQFYNGKADWDAIRSVREVTSVPLIANGDVDSVADAHEILRRSGADAVMVGRSCQGR
PWHAGVLAGTAAHPDAAGVARIFAEHYETLLEFYGVEVGLRTARKHAGWYLDRFATELPVSQKAAILTSTDTVFVRDGVA
AAIARSGDVAAREEIAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=230, Percent_Identity=32.1739130434783, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI239788462, Length=229, Percent_Identity=32.3144104803493, Blast_Score=118, Evalue=9e-27,
Organism=Homo sapiens, GI31742496, Length=237, Percent_Identity=31.6455696202532, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI8923374, Length=255, Percent_Identity=28.6274509803922, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI40807366, Length=217, Percent_Identity=29.9539170506912, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1789660, Length=291, Percent_Identity=46.7353951890034, Blast_Score=247, Evalue=1e-66,
Organism=Escherichia coli, GI1788462, Length=232, Percent_Identity=36.2068965517241, Blast_Score=114, Evalue=9e-27,
Organism=Escherichia coli, GI145693211, Length=252, Percent_Identity=25, Blast_Score=72, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17543114, Length=226, Percent_Identity=34.5132743362832, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17507177, Length=247, Percent_Identity=29.9595141700405, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI25144369, Length=255, Percent_Identity=27.843137254902, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17510279, Length=241, Percent_Identity=27.8008298755187, Blast_Score=87, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6323560, Length=262, Percent_Identity=30.9160305343511, Blast_Score=111, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6323433, Length=314, Percent_Identity=26.4331210191083, Blast_Score=96, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6323437, Length=234, Percent_Identity=31.6239316239316, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24585320, Length=275, Percent_Identity=32.3636363636364, Blast_Score=124, Evalue=9e-29,
Organism=Drosophila melanogaster, GI24580595, Length=216, Percent_Identity=33.7962962962963, Blast_Score=114, Evalue=7e-26,
Organism=Drosophila melanogaster, GI19920448, Length=216, Percent_Identity=33.7962962962963, Blast_Score=114, Evalue=7e-26,
Organism=Drosophila melanogaster, GI45549423, Length=263, Percent_Identity=31.5589353612167, Blast_Score=101, Evalue=6e-22,
Organism=Drosophila melanogaster, GI19921524, Length=224, Percent_Identity=30.8035714285714, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 36115; Mature: 36115

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLPSTALSSSFLIGNVQIRNRVALAPMSGVTDLPFRMLAWRFGAGFVVTEMVASRELVG
CCCCCCHHCCCEEEEEEEECCEEEECCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHH
NAAESWARLKNSGIDPHIVQLAGREAHWMAEAARIVEANGADIIDINMGCPAKKVTGGYS
HHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCEEEECCCCCHHHCCCCCC
GSALMRDPDHALSLIEATVKAATVPVTLKMRLGWDENSINAPLIARRAEEAGVKAITIHG
CCCCCCCHHHHHHHHHHHHHHEEEEEEEEEEECCCCCCCCCCHHHHHHHHCCCEEEEEEC
RTRMQFYNGKADWDAIRSVREVTSVPLIANGDVDSVADAHEILRRSGADAVMVGRSCQGR
CEEEEEECCCCCHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHCCCCEEEECCCCCCC
PWHAGVLAGTAAHPDAAGVARIFAEHYETLLEFYGVEVGLRTARKHAGWYLDRFATELPV
CCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHCCC
SQKAAILTSTDTVFVRDGVAAAIARSGDVAAREEIAA
CCCEEEEEECCEEEEECCHHHHHHCCCCCHHHHHHCC
>Mature Secondary Structure
MHLPSTALSSSFLIGNVQIRNRVALAPMSGVTDLPFRMLAWRFGAGFVVTEMVASRELVG
CCCCCCHHCCCEEEEEEEECCEEEECCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHH
NAAESWARLKNSGIDPHIVQLAGREAHWMAEAARIVEANGADIIDINMGCPAKKVTGGYS
HHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCEEEECCCCCHHHCCCCCC
GSALMRDPDHALSLIEATVKAATVPVTLKMRLGWDENSINAPLIARRAEEAGVKAITIHG
CCCCCCCHHHHHHHHHHHHHHEEEEEEEEEEECCCCCCCCCCHHHHHHHHCCCEEEEEEC
RTRMQFYNGKADWDAIRSVREVTSVPLIANGDVDSVADAHEILRRSGADAVMVGRSCQGR
CEEEEEECCCCCHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHCCCCEEEECCCCCCC
PWHAGVLAGTAAHPDAAGVARIFAEHYETLLEFYGVEVGLRTARKHAGWYLDRFATELPV
CCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHCCC
SQKAAILTSTDTVFVRDGVAAAIARSGDVAAREEIAA
CCCEEEEEECCEEEEECCHHHHHHCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8412703 [H]