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The map label for this gene is dppA [H]

Identifier: 150395555

GI number: 150395555

Start: 358968

End: 360563

Strand: Direct

Name: dppA [H]

Synonym: Smed_0329

Alternate gene names: 150395555

Gene position: 358968-360563 (Clockwise)

Preceding gene: 150395554

Following gene: 150395556

Centisome position: 9.49

GC content: 60.09

Gene sequence:

>1596_bases
ATGAAAAAGCTCACTACTCTTTTAGCGGCGACGGCGCTCGCCACGCTTATGGCCGGCACCGCCTGGTCGAAAACGTTCGT
CTTCTGCTCGGAAGGTTCGCCGGAGGGCTTTGATCCTGGCCTCTATACGGCCGGCACCACGTTCGACGCTGCCGCCCACA
CCGTTTACAGCCGTCTTCTCGAGTTCAAGAAGGGCACGACCGAAACGGAACCCGGGCTCGCCGAAAGCTGGACGATCTCC
GACGACGGCCTCGAATACACCTTCAAGCTGCGTCCCGGCGTCAAGTTTCAGACGACCGAATACTTCACTCCGACCCGTGA
ATTGAACGCCGACGATGTCGTCTTTTCGATCGAGCGCCAGTGGAAATCGGACCATCCATGGCATGGCTATGTGACCGGCG
GTTCCTGGGAATATTTTGCCGGCATGGGGCTGCCGGAACTGCTCGAGTCTGTCGAGAAGGTCGACGACATGACCGTCAAG
ATCAAGCTGAAGCGCAAGGAAGCGCCGTTCCTGGCCAATCTTGCCATGCCCTTCGCGTCGATCATGTCGAAGGAATATGC
CGACAAGCTGCAGGCCGAAGGCAAGATGAACCAGCTCAACCAGATGCCGCTCGGCACCGGTCCCTTCGCCTTTGTCGCCT
ATCAGCAGGACGCGGTCATCCGCTACAAGGCGCATCCGGAATTCTGGGGCGGAAAGCAGAAGATCGACGATCTGGTCTTT
GCGATCACCACGGACGCGGCCGTTCGCTTCCAGAAGCTGCAGGCCGGCGAATGCCACCTGATGCCCTATCCGAACGCGGC
GGATGTCGAGGCAATGAAGGCCGATCCGAACCTCAAGGTGATGGAGCAGGCCGGCCTCAACGTCGCCTATCTCGCCTATA
ACACGACGCAGCCGCCCTTCGATAAGCTCGAGGTGCGCAAGGCGCTGAACAAGGCGATCAACAAGGAGGCGATCGTCGAC
GCGGTCTTCCAGGGACAGGCGCAACCGGCGACCAATCCGATTCCGCCGACCATGTGGTCCTATAACGAGCAGATCGAAGA
CGACACCTATGATCCGGAAGCGGCGAAGAAGATGCTCGAGGATGCCGGCGTGAAAGATCTTTCGATGAAGGTCTGGGCGA
TGCCAGTGGCGCGTCCCTACATGCTCAACGCCCGTCGCGCCGCCGAACTGATGCAGGCCGACTTCGCCAAGGTCGGCGTC
AAGGTCGAAATCGTCTCCTACGAATGGGCCGAATATCTCGAGAAGTCCAAGGCGAAGGACCGCGACGGTGCCGTGATCCT
CGGCTGGACAGGCGACAACGGCGATCCGGACAACTTCCTCGACACGCTGCTCGGTTGCGACGCCGTCGGCGGCAACAACC
GCGCGCAATGGTGCAACCAGGAGTTCGACGAACTCGTCACGAAGGCGAAGGAAGCATCCGACGTCGCAGAGCGCACCAAG
CTCTATGAAGAGGCGCAGGTCGTCTTCAAGCGCGAAGCCCCCTGGGCTACGCTCGACCACTCGCTCTCCATCGTCCCGAT
GCGCAAGAATGTCGAAGGCTTCGTGCAGAGCCCGCTCGGCGACTTTGCTTTCGACGGCGTTGATATTGTAGAGTAA

Upstream 100 bases:

>100_bases
TGATAAGCCAAATGTCGTTCCATCCGCTCTTGACCGGGTGTAAACGTCATGCCGTGTCGGATAAATACGAGAACAAAAAA
CAAATCTGGGAAACAAGCTC

Downstream 100 bases:

>100_bases
TCTTATCGACTGATCCGAAGGGGGCGTTTGCCGCTGGCGAGCGCCCCTCTTCCTTTTTGCCAGTGCCGCCCCTTCTGCGG
CCACCTTCGGCATGAGGTTT

Product: extracellular solute-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: Dipeptide-binding protein; DBP [H]

Number of amino acids: Translated: 531; Mature: 531

Protein sequence:

>531_residues
MKKLTTLLAATALATLMAGTAWSKTFVFCSEGSPEGFDPGLYTAGTTFDAAAHTVYSRLLEFKKGTTETEPGLAESWTIS
DDGLEYTFKLRPGVKFQTTEYFTPTRELNADDVVFSIERQWKSDHPWHGYVTGGSWEYFAGMGLPELLESVEKVDDMTVK
IKLKRKEAPFLANLAMPFASIMSKEYADKLQAEGKMNQLNQMPLGTGPFAFVAYQQDAVIRYKAHPEFWGGKQKIDDLVF
AITTDAAVRFQKLQAGECHLMPYPNAADVEAMKADPNLKVMEQAGLNVAYLAYNTTQPPFDKLEVRKALNKAINKEAIVD
AVFQGQAQPATNPIPPTMWSYNEQIEDDTYDPEAAKKMLEDAGVKDLSMKVWAMPVARPYMLNARRAAELMQADFAKVGV
KVEIVSYEWAEYLEKSKAKDRDGAVILGWTGDNGDPDNFLDTLLGCDAVGGNNRAQWCNQEFDELVTKAKEASDVAERTK
LYEEAQVVFKREAPWATLDHSLSIVPMRKNVEGFVQSPLGDFAFDGVDIVE

Sequences:

>Translated_531_residues
MKKLTTLLAATALATLMAGTAWSKTFVFCSEGSPEGFDPGLYTAGTTFDAAAHTVYSRLLEFKKGTTETEPGLAESWTIS
DDGLEYTFKLRPGVKFQTTEYFTPTRELNADDVVFSIERQWKSDHPWHGYVTGGSWEYFAGMGLPELLESVEKVDDMTVK
IKLKRKEAPFLANLAMPFASIMSKEYADKLQAEGKMNQLNQMPLGTGPFAFVAYQQDAVIRYKAHPEFWGGKQKIDDLVF
AITTDAAVRFQKLQAGECHLMPYPNAADVEAMKADPNLKVMEQAGLNVAYLAYNTTQPPFDKLEVRKALNKAINKEAIVD
AVFQGQAQPATNPIPPTMWSYNEQIEDDTYDPEAAKKMLEDAGVKDLSMKVWAMPVARPYMLNARRAAELMQADFAKVGV
KVEIVSYEWAEYLEKSKAKDRDGAVILGWTGDNGDPDNFLDTLLGCDAVGGNNRAQWCNQEFDELVTKAKEASDVAERTK
LYEEAQVVFKREAPWATLDHSLSIVPMRKNVEGFVQSPLGDFAFDGVDIVE
>Mature_531_residues
MKKLTTLLAATALATLMAGTAWSKTFVFCSEGSPEGFDPGLYTAGTTFDAAAHTVYSRLLEFKKGTTETEPGLAESWTIS
DDGLEYTFKLRPGVKFQTTEYFTPTRELNADDVVFSIERQWKSDHPWHGYVTGGSWEYFAGMGLPELLESVEKVDDMTVK
IKLKRKEAPFLANLAMPFASIMSKEYADKLQAEGKMNQLNQMPLGTGPFAFVAYQQDAVIRYKAHPEFWGGKQKIDDLVF
AITTDAAVRFQKLQAGECHLMPYPNAADVEAMKADPNLKVMEQAGLNVAYLAYNTTQPPFDKLEVRKALNKAINKEAIVD
AVFQGQAQPATNPIPPTMWSYNEQIEDDTYDPEAAKKMLEDAGVKDLSMKVWAMPVARPYMLNARRAAELMQADFAKVGV
KVEIVSYEWAEYLEKSKAKDRDGAVILGWTGDNGDPDNFLDTLLGCDAVGGNNRAQWCNQEFDELVTKAKEASDVAERTK
LYEEAQVVFKREAPWATLDHSLSIVPMRKNVEGFVQSPLGDFAFDGVDIVE

Specific function: Dipeptide-binding protein of a transport system that can be subject to osmotic shock. DppA is also required for peptide chemotaxis [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=524, Percent_Identity=53.8167938931298, Blast_Score=583, Evalue=1e-168,
Organism=Escherichia coli, GI1787551, Length=504, Percent_Identity=36.9047619047619, Blast_Score=353, Evalue=1e-98,
Organism=Escherichia coli, GI1787052, Length=536, Percent_Identity=30.0373134328358, Blast_Score=192, Evalue=6e-50,
Organism=Escherichia coli, GI1787762, Length=515, Percent_Identity=26.4077669902913, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1789887, Length=465, Percent_Identity=25.8064516129032, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI1789397, Length=546, Percent_Identity=24.3589743589744, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1787495, Length=565, Percent_Identity=23.5398230088496, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87081878, Length=447, Percent_Identity=23.7136465324385, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 59126; Mature: 59126

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLTTLLAATALATLMAGTAWSKTFVFCSEGSPEGFDPGLYTAGTTFDAAAHTVYSRLL
CCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCEECCCCHHHHHHHHHHHHH
EFKKGTTETEPGLAESWTISDDGLEYTFKLRPGVKFQTTEYFTPTRELNADDVVFSIERQ
HHHCCCCCCCCCCCCEEEECCCCCEEEEEECCCCEEEECCCCCCCCCCCCCCEEEEEEHH
WKSDHPWHGYVTGGSWEYFAGMGLPELLESVEKVDDMTVKIKLKRKEAPFLANLAMPFAS
CCCCCCEEEEEECCCCHHHHCCCHHHHHHHHHHHCCEEEEEEEECCCCCHHHHHHHHHHH
IMSKEYADKLQAEGKMNQLNQMPLGTGPFAFVAYQQDAVIRYKAHPEFWGGKQKIDDLVF
HHHHHHHHHHHCCCCHHHHHCCCCCCCCEEEEEEECCEEEEEECCCCCCCCHHHHHHEEE
AITTDAAVRFQKLQAGECHLMPYPNAADVEAMKADPNLKVMEQAGLNVAYLAYNTTQPPF
EEECHHHHHHHHHCCCCEEEECCCCCCCCEEEECCCCCEEHHHCCCEEEEEEECCCCCCH
DKLEVRKALNKAINKEAIVDAVFQGQAQPATNPIPPTMWSYNEQIEDDTYDPEAAKKMLE
HHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
DAGVKDLSMKVWAMPVARPYMLNARRAAELMQADFAKVGVKVEIVSYEWAEYLEKSKAKD
HCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHCCCC
RDGAVILGWTGDNGDPDNFLDTLLGCDAVGGNNRAQWCNQEFDELVTKAKEASDVAERTK
CCCEEEEEECCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYEEAQVVFKREAPWATLDHSLSIVPMRKNVEGFVQSPLGDFAFDGVDIVE
HHHHHHHHHCCCCCCEECCCCEEEEEECCCHHHHHHCCCCHHHCCCEEECC
>Mature Secondary Structure
MKKLTTLLAATALATLMAGTAWSKTFVFCSEGSPEGFDPGLYTAGTTFDAAAHTVYSRLL
CCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCEECCCCHHHHHHHHHHHHH
EFKKGTTETEPGLAESWTISDDGLEYTFKLRPGVKFQTTEYFTPTRELNADDVVFSIERQ
HHHCCCCCCCCCCCCEEEECCCCCEEEEEECCCCEEEECCCCCCCCCCCCCCEEEEEEHH
WKSDHPWHGYVTGGSWEYFAGMGLPELLESVEKVDDMTVKIKLKRKEAPFLANLAMPFAS
CCCCCCEEEEEECCCCHHHHCCCHHHHHHHHHHHCCEEEEEEEECCCCCHHHHHHHHHHH
IMSKEYADKLQAEGKMNQLNQMPLGTGPFAFVAYQQDAVIRYKAHPEFWGGKQKIDDLVF
HHHHHHHHHHHCCCCHHHHHCCCCCCCCEEEEEEECCEEEEEECCCCCCCCHHHHHHEEE
AITTDAAVRFQKLQAGECHLMPYPNAADVEAMKADPNLKVMEQAGLNVAYLAYNTTQPPF
EEECHHHHHHHHHCCCCEEEECCCCCCCCEEEECCCCCEEHHHCCCEEEEEEECCCCCCH
DKLEVRKALNKAINKEAIVDAVFQGQAQPATNPIPPTMWSYNEQIEDDTYDPEAAKKMLE
HHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
DAGVKDLSMKVWAMPVARPYMLNARRAAELMQADFAKVGVKVEIVSYEWAEYLEKSKAKD
HCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHCCCC
RDGAVILGWTGDNGDPDNFLDTLLGCDAVGGNNRAQWCNQEFDELVTKAKEASDVAERTK
CCCEEEEEECCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYEEAQVVFKREAPWATLDHSLSIVPMRKNVEGFVQSPLGDFAFDGVDIVE
HHHHHHHHHCCCCCCEECCCCEEEEEECCCHHHHHHCCCCHHHCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1702779; 1956284; 7536291; 8041620; 9278503; 9298646; 9600841; 8563629; 8527431 [H]