Click here to switch to the map view.

The map label for this gene is aatA [H]

Identifier: 150395405

GI number: 150395405

Start: 195590

End: 196780

Strand: Direct

Name: aatA [H]

Synonym: Smed_0178

Alternate gene names: 150395405

Gene position: 195590-196780 (Clockwise)

Preceding gene: 150395404

Following gene: 150395406

Centisome position: 5.17

GC content: 64.65

Gene sequence:

>1191_bases
TTGCGCTACGCGTCGATCACATCCCGCCTCGCCGACCTCGGTTCCGGAAAATGGACGCTGCATGTTCGCGCGCGTCAGCT
GAAGGCGAGCGGGGCCGATATCATCGAACTGACCATCGGCGAACCCGATCTGCCGCCGGATCCCGCGTTGTTGGAGGAGT
GCCAGCGGGCCATGAACGCGGGGCGCTATCGGTATTCGAACGGCCGCGGTGAACCGGCGGTCGTTGCGGCCCTGACGGAG
AAGTACCGGCGCCGCCGCTCCGGCGTTACGAAGGAAAATATTCTCTGCTTTCCGGGAACGCAGACGGCGCTCTTCGCCGT
CATGTTCGGCCTTGCGGAGGCAGGCGACGGCGTGCTCGTCGGAGACCCGCTCTACGCCACCTATGAAGGGGTGATCCGCT
CGACGGGCGCCCATCCGGTGTTCGTGCCGCTCAACCCGCAACACGGCTTTCACATGCAGGCCGAGGACCTGGAGAAGGCG
GTCACGCCCGAGTGCCGGGTGCTGCTCCTCAACACCCCGCACAATCCGACCGGTGCCGTCCTGACGCCGGAAGAGATTGC
GGCGATCGGTGCGGTCGCACGACGTCACGATCTCTGGATCGTCTGCGACGAAGTGTATGAGGAACTCGTCTTCGACGCCG
CCTTTGCTTCGCCCTTCGACAATCCTGATCTCGCCGAACGAACAGTGGTCGTCTCGTCCATCTCCAAGTCGCATGCAGCA
ACGGGCTTTCGCAGCGGCTGGGCGGTTGGACCGGCCGAGTTCACCGAGCGCCTGCTGCCGATTTCCGAGACCATGCTCTT
TGGTCAGCAGCCGTTCATCGCAGATATGACCGCCTACGCGCTGACGCACGAGATCGGCACGGCGAGACAAATGCGGGAAT
CCTACAGTCACCGCGCCCGACTGACCATCGACGGCCTTGCGGGAGTGCCCGGCGTTTCGGTGCTGCCGCCCGAAGCGGGT
ATGTTCGCGCTGATCGACGTCTCGGGCACAAGCCTCTCCGGCGAAGCCTTCGCCTGGGCGCTCCTCGAGGAGGAGGGGGT
TGCAGTCATGCCGGGGTCTTCTTTCGGCGACACGGCACGAAACTTCCTTCGCGTGAGCCTCACCGTTCCCGATGATGTCA
TTGAAGAGGCATGCCGCCGCATTGCTGCTCTCGCCCTACGCTGCACCGCCTGCAGGGAGCGCCGCGCATGA

Upstream 100 bases:

>100_bases
TTTTCGCGAGGGCGAGCTGGTCAGCATCGCCATGGCGTCGGTTGAAGCCCTGCTCGGTCTTCCCATCAAAAAAACAGAAA
ACAAATAAGGGAGCTACTTT

Downstream 100 bases:

>100_bases
GCGTCGAATTGAAGACCGTCGGCGAAACCCTCGTCGACCTTCTGGAGGCAAACGGCGTGGAGGTCGTATTCGGCATTCCC
GGCGTGCACACGGTCGAGCT

Product: class I and II aminotransferase

Products: NA

Alternate protein names: AspAT; Transaminase A [H]

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MRYASITSRLADLGSGKWTLHVRARQLKASGADIIELTIGEPDLPPDPALLEECQRAMNAGRYRYSNGRGEPAVVAALTE
KYRRRRSGVTKENILCFPGTQTALFAVMFGLAEAGDGVLVGDPLYATYEGVIRSTGAHPVFVPLNPQHGFHMQAEDLEKA
VTPECRVLLLNTPHNPTGAVLTPEEIAAIGAVARRHDLWIVCDEVYEELVFDAAFASPFDNPDLAERTVVVSSISKSHAA
TGFRSGWAVGPAEFTERLLPISETMLFGQQPFIADMTAYALTHEIGTARQMRESYSHRARLTIDGLAGVPGVSVLPPEAG
MFALIDVSGTSLSGEAFAWALLEEEGVAVMPGSSFGDTARNFLRVSLTVPDDVIEEACRRIAALALRCTACRERRA

Sequences:

>Translated_396_residues
MRYASITSRLADLGSGKWTLHVRARQLKASGADIIELTIGEPDLPPDPALLEECQRAMNAGRYRYSNGRGEPAVVAALTE
KYRRRRSGVTKENILCFPGTQTALFAVMFGLAEAGDGVLVGDPLYATYEGVIRSTGAHPVFVPLNPQHGFHMQAEDLEKA
VTPECRVLLLNTPHNPTGAVLTPEEIAAIGAVARRHDLWIVCDEVYEELVFDAAFASPFDNPDLAERTVVVSSISKSHAA
TGFRSGWAVGPAEFTERLLPISETMLFGQQPFIADMTAYALTHEIGTARQMRESYSHRARLTIDGLAGVPGVSVLPPEAG
MFALIDVSGTSLSGEAFAWALLEEEGVAVMPGSSFGDTARNFLRVSLTVPDDVIEEACRRIAALALRCTACRERRA
>Mature_396_residues
MRYASITSRLADLGSGKWTLHVRARQLKASGADIIELTIGEPDLPPDPALLEECQRAMNAGRYRYSNGRGEPAVVAALTE
KYRRRRSGVTKENILCFPGTQTALFAVMFGLAEAGDGVLVGDPLYATYEGVIRSTGAHPVFVPLNPQHGFHMQAEDLEKA
VTPECRVLLLNTPHNPTGAVLTPEEIAAIGAVARRHDLWIVCDEVYEELVFDAAFASPFDNPDLAERTVVVSSISKSHAA
TGFRSGWAVGPAEFTERLLPISETMLFGQQPFIADMTAYALTHEIGTARQMRESYSHRARLTIDGLAGVPGVSVLPPEAG
MFALIDVSGTSLSGEAFAWALLEEEGVAVMPGSSFGDTARNFLRVSLTVPDDVIEEACRRIAALALRCTACRERRA

Specific function: Required for symbiotic nitrogen fixation [H]

COG id: COG0436

COG function: function code E; Aspartate/tyrosine/aromatic aminotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI56713256, Length=426, Percent_Identity=25.5868544600939, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI56713254, Length=425, Percent_Identity=25.6470588235294, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI95147551, Length=274, Percent_Identity=28.8321167883212, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI169881279, Length=274, Percent_Identity=28.8321167883212, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI4507369, Length=377, Percent_Identity=23.8726790450928, Blast_Score=111, Evalue=9e-25,
Organism=Homo sapiens, GI169881281, Length=161, Percent_Identity=31.055900621118, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI149944424, Length=252, Percent_Identity=25.7936507936508, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1786816, Length=357, Percent_Identity=28.5714285714286, Blast_Score=124, Evalue=9e-30,
Organism=Escherichia coli, GI1788722, Length=363, Percent_Identity=29.4765840220386, Blast_Score=118, Evalue=8e-28,
Organism=Escherichia coli, GI1788627, Length=377, Percent_Identity=25.7294429708223, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1787909, Length=367, Percent_Identity=21.5258855585831, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1790797, Length=308, Percent_Identity=24.6753246753247, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1787710, Length=304, Percent_Identity=24.0131578947368, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI71994472, Length=340, Percent_Identity=27.3529411764706, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI71994476, Length=340, Percent_Identity=27.3529411764706, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17567369, Length=386, Percent_Identity=25.6476683937824, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17567663, Length=377, Percent_Identity=24.9336870026525, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6322401, Length=393, Percent_Identity=27.9898218829517, Blast_Score=147, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6320317, Length=344, Percent_Identity=25.5813953488372, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI28573069, Length=300, Percent_Identity=28.6666666666667, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24646114, Length=300, Percent_Identity=28.6666666666667, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI28573067, Length=300, Percent_Identity=28.6666666666667, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI28573065, Length=300, Percent_Identity=28.6666666666667, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI18859735, Length=379, Percent_Identity=26.1213720316623, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001176
- InterPro:   IPR004839
- InterPro:   IPR004838
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =2.6.1.1 [H]

Molecular weight: Translated: 42856; Mature: 42856

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYASITSRLADLGSGKWTLHVRARQLKASGADIIELTIGEPDLPPDPALLEECQRAMNA
CCCHHHHHHHHHCCCCEEEEEEEEEEHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHC
GRYRYSNGRGEPAVVAALTEKYRRRRSGVTKENILCFPGTQTALFAVMFGLAEAGDGVLV
CCEEECCCCCCHHHHHHHHHHHHHHHCCCCHHCEEEECCCHHHHHHHHHHHHHCCCCEEE
GDPLYATYEGVIRSTGAHPVFVPLNPQHGFHMQAEDLEKAVTPECRVLLLNTPHNPTGAV
CCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHCCCCEEEEEEECCCCCCCCE
LTPEEIAAIGAVARRHDLWIVCDEVYEELVFDAAFASPFDNPDLAERTVVVSSISKSHAA
ECHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHC
TGFRSGWAVGPAEFTERLLPISETMLFGQQPFIADMTAYALTHEIGTARQMRESYSHRAR
CCCCCCCCCCHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LTIDGLAGVPGVSVLPPEAGMFALIDVSGTSLSGEAFAWALLEEEGVAVMPGSSFGDTAR
EEECCCCCCCCCEECCCCCCEEEEEEECCCCCCCCCEEEEEECCCCEEEECCCCCCHHHH
NFLRVSLTVPDDVIEEACRRIAALALRCTACRERRA
CEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRYASITSRLADLGSGKWTLHVRARQLKASGADIIELTIGEPDLPPDPALLEECQRAMNA
CCCHHHHHHHHHCCCCEEEEEEEEEEHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHC
GRYRYSNGRGEPAVVAALTEKYRRRRSGVTKENILCFPGTQTALFAVMFGLAEAGDGVLV
CCEEECCCCCCHHHHHHHHHHHHHHHCCCCHHCEEEECCCHHHHHHHHHHHHHCCCCEEE
GDPLYATYEGVIRSTGAHPVFVPLNPQHGFHMQAEDLEKAVTPECRVLLLNTPHNPTGAV
CCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHCCCCEEEEEEECCCCCCCCE
LTPEEIAAIGAVARRHDLWIVCDEVYEELVFDAAFASPFDNPDLAERTVVVSSISKSHAA
ECHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHC
TGFRSGWAVGPAEFTERLLPISETMLFGQQPFIADMTAYALTHEIGTARQMRESYSHRAR
CCCCCCCCCCHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LTIDGLAGVPGVSVLPPEAGMFALIDVSGTSLSGEAFAWALLEEEGVAVMPGSSFGDTAR
EEECCCCCCCCCEECCCCCCEEEEEEECCCCCCCCCEEEEEECCCCEEEECCCCCCHHHH
NFLRVSLTVPDDVIEEACRRIAALALRCTACRERRA
CEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2019560; 8096210; 11481430 [H]