Click here to switch to the map view.

The map label for this gene is ilvB [H]

Identifier: 150395406

GI number: 150395406

Start: 196777

End: 198390

Strand: Direct

Name: ilvB [H]

Synonym: Smed_0179

Alternate gene names: 150395406

Gene position: 196777-198390 (Clockwise)

Preceding gene: 150395405

Following gene: 150395407

Centisome position: 5.2

GC content: 65.55

Gene sequence:

>1614_bases
ATGAGCGTCGAATTGAAGACCGTCGGCGAAACCCTCGTCGACCTTCTGGAGGCAAACGGCGTGGAGGTCGTATTCGGCAT
TCCCGGCGTGCACACGGTCGAGCTTTATCGTGGCCTTTCCGCATCGAAAATCCGCCACGTGACGCCTCGCCACGAACAGG
GTGCCGGCTTCATGGCCGATGGCTATGCACGCGTCAGCGGCAAGCCGGGCGTGGCGCTCGTGATCACCGGCCCGGGCCTC
ACCAACACGATCACCGCCATGGCGCAGGCCCGGCAGGACTCGATCCCGATGCTGGTGATCTCTGGCGTCAACCGCCGCGA
TTCGCTCGGTCATGGGCAGGGGTTGCTCCACGAACTGCCGGATCAGCACGGAATGATCAAGACGCTGGCACTTTATTCGC
ACACGCTGCTCAACCCCGCGGATCTGCCGCTCGTCGTGGACCGCGCCTTTGCGGTTCTGCTTTCCGGCCGTCCGGGCCCG
GTCCACATCGAGATTCCGACCGACGTGATGGCGCTCAGGATCGAGGACCGGCCCTCGGCGCCGGCAACGGCAACCCGGCC
GCGCTCCGACAGCGAAACGCTGCAGAGGGCAGCGATCCTTTGTGCGGAGGCCGCCCGCCCGGTCATCGTGTGCGGCGGCG
GTGCCCTGACGGCGGAAGCGGAAGTGCGTGAACTTGCCGAAAGGATCGGCGCGCCGGTCGTCACGACGGTCAACGCCCGC
GGCATTCTTGCCGGCCATCCGCTCCGGGTGCCGGCAAGCCCCAGCCTCAAGGCGGTGCGCGCCCTTTTGCGAGAGGCGGA
TCTCGTGCTGGCGCTCGGCACCGAGATGGGCCAGACGGATTACGATCTCTACGCCGATGGCGGTTTTCCGCCGCTGAAGA
ACCTTGTTCGCACCGACATCGATGCGGCGCAGCTCGCACGCGGGCCGCAGGCAGCGCTTTCGATCCTTTCAGGCGCGAGG
GCCGCCACCGCGGGTATTCTGGGCTTCCTGCCGGATCATGCCGCGGCCAGGGACGGCATTCTTCGGGCGGAGGCCACGCG
CAAGGCGGCATTGAGAGAGCTGACACCGAAAATGCTTGCCGAGGTCGGCGTCGTCGAGGCGATCTACCGCACGCTTCCAG
ATTGCATCATCGTCGGCGATTCGACTCAGGCCGTCTATGCCGGGAACCTCTATTGCGATGCGCCGCGCCAAAGGGCCTGG
TTCAATTCGGCGACGGGCTACGGCTCCCTCGGCTATGCGCCCCCCGCCGCGGTCGGTGCTGCGGTTGCCGATCGGGAGCG
ACCGGTCATATGTCTTGTCGGCGACGGCGGATTTCAGTTTTCCCTGGCGGAGATCGGCTCGGCAGTGGATGCGGCCGCCC
AGGTCGTTTTCCTCGTCTGGAATAATGACGGGTACCGGGAAATCGAGTCCTACATGGTCGAAAACGGAATCACGCCGGAA
GGCGTCAAACCCTCCGCACCCGATTTCCTGCTTGCTGCAAAGGCCTATGGCGTGCCTGCGGAGCGACTGGCGACGGTGAA
GGATCTGCCGCGTGCGCTTGCCGATGCGGTGTCGCGATCGGGGCCTTCGCTGATCGAGATCCATCAGGAAAGGACTGCCG
GAGCCACAGCGTGA

Upstream 100 bases:

>100_bases
TTCGCGTGAGCCTCACCGTTCCCGATGATGTCATTGAAGAGGCATGCCGCCGCATTGCTGCTCTCGCCCTACGCTGCACC
GCCTGCAGGGAGCGCCGCGC

Downstream 100 bases:

>100_bases
CGCCCTGGTGAAGGGCTCATCCAGCCCCGGATTTGACTTTTCCGGCCCGGAGTGGTGTTAAGTGCGCACATGCTCGAATC
CGGCCACGTTCTTCGTTTAG

Product: hypothetical protein

Products: NA

Alternate protein names: AHAS-I; Acetohydroxy-acid synthase I large subunit; ALS-I [H]

Number of amino acids: Translated: 537; Mature: 536

Protein sequence:

>537_residues
MSVELKTVGETLVDLLEANGVEVVFGIPGVHTVELYRGLSASKIRHVTPRHEQGAGFMADGYARVSGKPGVALVITGPGL
TNTITAMAQARQDSIPMLVISGVNRRDSLGHGQGLLHELPDQHGMIKTLALYSHTLLNPADLPLVVDRAFAVLLSGRPGP
VHIEIPTDVMALRIEDRPSAPATATRPRSDSETLQRAAILCAEAARPVIVCGGGALTAEAEVRELAERIGAPVVTTVNAR
GILAGHPLRVPASPSLKAVRALLREADLVLALGTEMGQTDYDLYADGGFPPLKNLVRTDIDAAQLARGPQAALSILSGAR
AATAGILGFLPDHAAARDGILRAEATRKAALRELTPKMLAEVGVVEAIYRTLPDCIIVGDSTQAVYAGNLYCDAPRQRAW
FNSATGYGSLGYAPPAAVGAAVADRERPVICLVGDGGFQFSLAEIGSAVDAAAQVVFLVWNNDGYREIESYMVENGITPE
GVKPSAPDFLLAAKAYGVPAERLATVKDLPRALADAVSRSGPSLIEIHQERTAGATA

Sequences:

>Translated_537_residues
MSVELKTVGETLVDLLEANGVEVVFGIPGVHTVELYRGLSASKIRHVTPRHEQGAGFMADGYARVSGKPGVALVITGPGL
TNTITAMAQARQDSIPMLVISGVNRRDSLGHGQGLLHELPDQHGMIKTLALYSHTLLNPADLPLVVDRAFAVLLSGRPGP
VHIEIPTDVMALRIEDRPSAPATATRPRSDSETLQRAAILCAEAARPVIVCGGGALTAEAEVRELAERIGAPVVTTVNAR
GILAGHPLRVPASPSLKAVRALLREADLVLALGTEMGQTDYDLYADGGFPPLKNLVRTDIDAAQLARGPQAALSILSGAR
AATAGILGFLPDHAAARDGILRAEATRKAALRELTPKMLAEVGVVEAIYRTLPDCIIVGDSTQAVYAGNLYCDAPRQRAW
FNSATGYGSLGYAPPAAVGAAVADRERPVICLVGDGGFQFSLAEIGSAVDAAAQVVFLVWNNDGYREIESYMVENGITPE
GVKPSAPDFLLAAKAYGVPAERLATVKDLPRALADAVSRSGPSLIEIHQERTAGATA
>Mature_536_residues
SVELKTVGETLVDLLEANGVEVVFGIPGVHTVELYRGLSASKIRHVTPRHEQGAGFMADGYARVSGKPGVALVITGPGLT
NTITAMAQARQDSIPMLVISGVNRRDSLGHGQGLLHELPDQHGMIKTLALYSHTLLNPADLPLVVDRAFAVLLSGRPGPV
HIEIPTDVMALRIEDRPSAPATATRPRSDSETLQRAAILCAEAARPVIVCGGGALTAEAEVRELAERIGAPVVTTVNARG
ILAGHPLRVPASPSLKAVRALLREADLVLALGTEMGQTDYDLYADGGFPPLKNLVRTDIDAAQLARGPQAALSILSGARA
ATAGILGFLPDHAAARDGILRAEATRKAALRELTPKMLAEVGVVEAIYRTLPDCIIVGDSTQAVYAGNLYCDAPRQRAWF
NSATGYGSLGYAPPAAVGAAVADRERPVICLVGDGGFQFSLAEIGSAVDAAAQVVFLVWNNDGYREIESYMVENGITPEG
VKPSAPDFLLAAKAYGVPAERLATVKDLPRALADAVSRSGPSLIEIHQERTAGATA

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=562, Percent_Identity=25.4448398576512, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI21361361, Length=523, Percent_Identity=27.7246653919694, Blast_Score=137, Evalue=3e-32,
Organism=Escherichia coli, GI1790104, Length=536, Percent_Identity=32.8358208955224, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI87081685, Length=541, Percent_Identity=29.2051756007394, Blast_Score=197, Evalue=2e-51,
Organism=Escherichia coli, GI1786717, Length=474, Percent_Identity=28.9029535864979, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1788716, Length=558, Percent_Identity=26.7025089605735, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1787096, Length=550, Percent_Identity=23.6363636363636, Blast_Score=100, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17542570, Length=572, Percent_Identity=27.2727272727273, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17531299, Length=558, Percent_Identity=23.8351254480287, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17531301, Length=558, Percent_Identity=23.8351254480287, Blast_Score=96, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6323755, Length=570, Percent_Identity=27.3684210526316, Blast_Score=181, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6320816, Length=553, Percent_Identity=26.5822784810127, Blast_Score=117, Evalue=5e-27,
Organism=Drosophila melanogaster, GI19922626, Length=484, Percent_Identity=28.099173553719, Blast_Score=146, Evalue=4e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 56320; Mature: 56189

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVELKTVGETLVDLLEANGVEVVFGIPGVHTVELYRGLSASKIRHVTPRHEQGAGFMAD
CCEEHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHCCCCHHHHHCCCCCCCCCCCEEEC
GYARVSGKPGVALVITGPGLTNTITAMAQARQDSIPMLVISGVNRRDSLGHGQGLLHELP
CEEEECCCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHCC
DQHGMIKTLALYSHTLLNPADLPLVVDRAFAVLLSGRPGPVHIEIPTDVMALRIEDRPSA
CCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHEECCCCCEEEEEECCCEEEEEECCCCCC
PATATRPRSDSETLQRAAILCAEAARPVIVCGGGALTAEAEVRELAERIGAPVVTTVNAR
CCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCEECHHHHHHHHHHHCCCEEEEECCC
GILAGHPLRVPASPSLKAVRALLREADLVLALGTEMGQTDYDLYADGGFPPLKNLVRTDI
EEEECCCEECCCCCCHHHHHHHHHHCCEEEEECCCCCCCCCEEEECCCCCHHHHHHHCCC
DAAQLARGPQAALSILSGARAATAGILGFLPDHAAARDGILRAEATRKAALRELTPKMLA
CHHHHCCCHHHHHHHHHCCHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHCHHHHH
EVGVVEAIYRTLPDCIIVGDSTQAVYAGNLYCDAPRQRAWFNSATGYGSLGYAPPAAVGA
HHHHHHHHHHHCCCEEEECCCCCEEEECCEEECCCHHHHHCCCCCCCCCCCCCCHHHHHH
AVADRERPVICLVGDGGFQFSLAEIGSAVDAAAQVVFLVWNNDGYREIESYMVENGITPE
HHHCCCCCEEEEECCCCCEEEHHHHCHHHHCCCEEEEEEECCCCHHHHHHHHHHCCCCCC
GVKPSAPDFLLAAKAYGVPAERLATVKDLPRALADAVSRSGPSLIEIHQERTAGATA
CCCCCCCCCEEEHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHCCCCCC
>Mature Secondary Structure 
SVELKTVGETLVDLLEANGVEVVFGIPGVHTVELYRGLSASKIRHVTPRHEQGAGFMAD
CEEHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHCCCCHHHHHCCCCCCCCCCCEEEC
GYARVSGKPGVALVITGPGLTNTITAMAQARQDSIPMLVISGVNRRDSLGHGQGLLHELP
CEEEECCCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHCC
DQHGMIKTLALYSHTLLNPADLPLVVDRAFAVLLSGRPGPVHIEIPTDVMALRIEDRPSA
CCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHEECCCCCEEEEEECCCEEEEEECCCCCC
PATATRPRSDSETLQRAAILCAEAARPVIVCGGGALTAEAEVRELAERIGAPVVTTVNAR
CCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCEECHHHHHHHHHHHCCCEEEEECCC
GILAGHPLRVPASPSLKAVRALLREADLVLALGTEMGQTDYDLYADGGFPPLKNLVRTDI
EEEECCCEECCCCCCHHHHHHHHHHCCEEEEECCCCCCCCCEEEECCCCCHHHHHHHCCC
DAAQLARGPQAALSILSGARAATAGILGFLPDHAAARDGILRAEATRKAALRELTPKMLA
CHHHHCCCHHHHHHHHHCCHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHCHHHHH
EVGVVEAIYRTLPDCIIVGDSTQAVYAGNLYCDAPRQRAWFNSATGYGSLGYAPPAAVGA
HHHHHHHHHHHCCCEEEECCCCCEEEECCEEECCCHHHHHCCCCCCCCCCCCCCHHHHHH
AVADRERPVICLVGDGGFQFSLAEIGSAVDAAAQVVFLVWNNDGYREIESYMVENGITPE
HHHCCCCCEEEEECCCCCEEEHHHHCHHHHCCCEEEEEEECCCCHHHHHHHHHHCCCCCC
GVKPSAPDFLLAAKAYGVPAERLATVKDLPRALADAVSRSGPSLIEIHQERTAGATA
CCCCCCCCCEEEHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2989782; 2989781; 7686882; 9278503 [H]