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The map label for this gene is mscL [H]

Identifier: 150395393

GI number: 150395393

Start: 184078

End: 184506

Strand: Direct

Name: mscL [H]

Synonym: Smed_0166

Alternate gene names: 150395393

Gene position: 184078-184506 (Clockwise)

Preceding gene: 150395391

Following gene: 150395394

Centisome position: 4.87

GC content: 58.97

Gene sequence:

>429_bases
ATGCTGAACGAATTCAAGGAGTTTATCGCCCGCGGCAATGTCATGGACCTTGCGGTCGGCGTGATCATCGGTGCGGCCTT
TAGCAAGATCGTCGATTCGGTCGTCAACGACCTTGTCATGCCCGTCGTCGGAGCGCTCACCGGGGGCGGCTTTGACTTCT
CCGATTATTTCCTGCCTCTCTCGGGGAACGTCACCGCGCAGACGCTTGCGGCCGCACGTGAACAGGGCGCCGTCTTCGCT
TACGGCAATTTCATCACCGTGTTCATCAACTTCCTGATCCTTGCCTGGATCATCTTTCTTCTGATCAAGCTGGTGAATCG
CGCCCGCGCCTCGGTCGAGCGGGAAAAGGCTCCGGAACCGGCTGCACCTCCGCCGCAGGACATCCTGCTTCTTTCCGAAA
TTCGCGATCTCCTGAAGCAGCGCGCCTGA

Upstream 100 bases:

>100_bases
AGTGCGATTTCCTCACGCGGGGCTTTGGCCAGATTCCTACCGGCGGTTGCCTTGCAGGACAGGTGCGAGTGGCCGTTCGG
GGATTTTTACGGAGAGAGGC

Downstream 100 bases:

>100_bases
GCCGTTCATCGGCCGGGCGCTCGCCGGGCCGCGCGAAACATCACGGAAATCGATAAACAGGTCAGAGAAATTCGCGTGAT
CGGCGACGCCGAAGCGTTTA

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 142; Mature: 142

Protein sequence:

>142_residues
MLNEFKEFIARGNVMDLAVGVIIGAAFSKIVDSVVNDLVMPVVGALTGGGFDFSDYFLPLSGNVTAQTLAAAREQGAVFA
YGNFITVFINFLILAWIIFLLIKLVNRARASVEREKAPEPAAPPPQDILLLSEIRDLLKQRA

Sequences:

>Translated_142_residues
MLNEFKEFIARGNVMDLAVGVIIGAAFSKIVDSVVNDLVMPVVGALTGGGFDFSDYFLPLSGNVTAQTLAAAREQGAVFA
YGNFITVFINFLILAWIIFLLIKLVNRARASVEREKAPEPAAPPPQDILLLSEIRDLLKQRA
>Mature_142_residues
MLNEFKEFIARGNVMDLAVGVIIGAAFSKIVDSVVNDLVMPVVGALTGGGFDFSDYFLPLSGNVTAQTLAAAREQGAVFA
YGNFITVFINFLILAWIIFLLIKLVNRARASVEREKAPEPAAPPPQDILLLSEIRDLLKQRA

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell [H]

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family [H]

Homologues:

Organism=Escherichia coli, GI1789686, Length=145, Percent_Identity=46.2068965517241, Blast_Score=106, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019823
- InterPro:   IPR001185 [H]

Pfam domain/function: PF01741 MscL [H]

EC number: NA

Molecular weight: Translated: 15424; Mature: 15424

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNEFKEFIARGNVMDLAVGVIIGAAFSKIVDSVVNDLVMPVVGALTGGGFDFSDYFLPL
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCEECC
SGNVTAQTLAAAREQGAVFAYGNFITVFINFLILAWIIFLLIKLVNRARASVEREKAPEP
CCCHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AAPPPQDILLLSEIRDLLKQRA
CCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLNEFKEFIARGNVMDLAVGVIIGAAFSKIVDSVVNDLVMPVVGALTGGGFDFSDYFLPL
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCEECC
SGNVTAQTLAAAREQGAVFAYGNFITVFINFLILAWIIFLLIKLVNRARASVEREKAPEP
CCCHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AAPPPQDILLLSEIRDLLKQRA
CCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]