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The map label for this gene is merA [H]

Identifier: 150395312

GI number: 150395312

Start: 92349

End: 93770

Strand: Direct

Name: merA [H]

Synonym: Smed_0084

Alternate gene names: 150395312

Gene position: 92349-93770 (Clockwise)

Preceding gene: 150395311

Following gene: 150395313

Centisome position: 2.44

GC content: 64.35

Gene sequence:

>1422_bases
GTGGTGAAGCAATTGACACCGGACATCTGCGTAATTGGCGGCGGTGCAGCCGGGCTCACGGTAGCCGCCGGAGCTGCCGC
CTTCGGCGTGCCCGTCGTGCTGGTGGAAAAGGGGCCGATGGGCGGCGACTGCCTGAATCACGGCTGCGTGCCTTCGAAGG
CTCTGATCGCGGCGTCAAGGCATGCGCATTCCATCAGGGTTGCAGCGGAATTCGGCATCGCCGCAGCAGGGCCCGTCATC
GATCAGGAACGCCTGACGGCGCGCATCCAGTCCGTCATAGTCGGCATTGCACCGCATGATTCCGTCGAGCGCTTCACGAG
CCTCGGGGTCGAGGTCATCAAGGATGAGGCTTGCTTCGTCGATAACCGCACCATCGCTGCCGGTGACCGCCTCATCCGCG
CCCGGCGTTTCGTGATCGCTACCGGCTCATCGCCGGCAATTCCCCCGATCCCGGGGCTGGCGGAGACGCCGTTCCTCACC
AATGAAACGCTCTTCAGTCTGAAACGCCTGCCGCGCCATCTTGTCGTCATCGGCGGGGGGCCGGTCGGACTCGAAATGGC
TGGGGCTCACCGCCGCCTGGGCGTCGAGGTGACGGTCGTGGACAAGACGGAGGCCCTGTCCGGGCAGGATCCGGAACTGG
CGGCGATTGTGCTCGACGGGCTGAGAGCGGAAGGCGTGCACCTCCATGAACGCACGGCGATCCACGCGGTCGAACGGACG
GAGCGTGGTATCCGGCTGCGGTGCGAAAACGGGAACGGTCCTTTCGGGATCGAAGGAAGCGATCTGCTGGTGGCCGCCGG
CCGCGCGCCCGTTCATGCATCTCTCGGTCTCGATGCGGCAGGGATCAGACACGATCCGAAGCGTATCGAGGTCGGTCCGA
ATCTAAGGACCAGCAACCGCCGGGTCTATGCGATCGGCGATGCGGCGGGTGGCCTATTCACGCATCAGGCGAGCTATCAT
GCCCGCCTGGTGCTGCAGCAGATACTCTTCCGCCTGCCGGCGCGCGAGAAGCCGTTCATCGTTCCACGGGTGATCTTCAC
CGAGCCCGAACTGGCTCATGTGGGGCTGACGGAAGAGCGTGCGCGCGAAGCAGCCCCGGGGGCGACGATCCTTCGCCTGG
ATTATTCCGCGAACGACCGCTCCCGTACCGATGGTCTTGGCCGAGGGTTGATCAAGGTGGTCGTCGGCAGGCGGGGCCGG
GTGCTCGGTGCAGCCATTGCGGGTTCCGGAGCCGGGGAGATGATCAATCTCTGGGCCTTCGCCGTTGCCAATCGCCTGAC
CCTCAAGCATTTCCAGACTTATGTCGCACCCTATCCGACCCTCTCGGAGATTGGAAAACAGGCGGCGATCTCCTATTATT
CCCCGATGGCGCGAAATCGCCTTCTGCGGACCGTGCTTCGGTTCCTCAGACATTTCGGCTGA

Upstream 100 bases:

>100_bases
CTCATCGCCCTTGCGCTGATTGCTACATTGCCCCTGGCATTCAGGCTTATACAGTCGCGCCGCAAAGAAGCGTGACGGAG
ATACGGATAGGGTAGAGGGC

Downstream 100 bases:

>100_bases
CGGCAGGGAATATTGAGAGTCATGGTGGAAGATGCGCGTCCGGCCGATCCGAGTGCGGCGCCACGAGCGGCGGCCGGCTA
TCTTGGTGGCCTTTCCGGCA

Product: pyridine nucleotide-disulfide oxidoreductase dimerisation region

Products: NA

Alternate protein names: Hg(II) reductase [H]

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MVKQLTPDICVIGGGAAGLTVAAGAAAFGVPVVLVEKGPMGGDCLNHGCVPSKALIAASRHAHSIRVAAEFGIAAAGPVI
DQERLTARIQSVIVGIAPHDSVERFTSLGVEVIKDEACFVDNRTIAAGDRLIRARRFVIATGSSPAIPPIPGLAETPFLT
NETLFSLKRLPRHLVVIGGGPVGLEMAGAHRRLGVEVTVVDKTEALSGQDPELAAIVLDGLRAEGVHLHERTAIHAVERT
ERGIRLRCENGNGPFGIEGSDLLVAAGRAPVHASLGLDAAGIRHDPKRIEVGPNLRTSNRRVYAIGDAAGGLFTHQASYH
ARLVLQQILFRLPAREKPFIVPRVIFTEPELAHVGLTEERAREAAPGATILRLDYSANDRSRTDGLGRGLIKVVVGRRGR
VLGAAIAGSGAGEMINLWAFAVANRLTLKHFQTYVAPYPTLSEIGKQAAISYYSPMARNRLLRTVLRFLRHFG

Sequences:

>Translated_473_residues
MVKQLTPDICVIGGGAAGLTVAAGAAAFGVPVVLVEKGPMGGDCLNHGCVPSKALIAASRHAHSIRVAAEFGIAAAGPVI
DQERLTARIQSVIVGIAPHDSVERFTSLGVEVIKDEACFVDNRTIAAGDRLIRARRFVIATGSSPAIPPIPGLAETPFLT
NETLFSLKRLPRHLVVIGGGPVGLEMAGAHRRLGVEVTVVDKTEALSGQDPELAAIVLDGLRAEGVHLHERTAIHAVERT
ERGIRLRCENGNGPFGIEGSDLLVAAGRAPVHASLGLDAAGIRHDPKRIEVGPNLRTSNRRVYAIGDAAGGLFTHQASYH
ARLVLQQILFRLPAREKPFIVPRVIFTEPELAHVGLTEERAREAAPGATILRLDYSANDRSRTDGLGRGLIKVVVGRRGR
VLGAAIAGSGAGEMINLWAFAVANRLTLKHFQTYVAPYPTLSEIGKQAAISYYSPMARNRLLRTVLRFLRHFG
>Mature_473_residues
MVKQLTPDICVIGGGAAGLTVAAGAAAFGVPVVLVEKGPMGGDCLNHGCVPSKALIAASRHAHSIRVAAEFGIAAAGPVI
DQERLTARIQSVIVGIAPHDSVERFTSLGVEVIKDEACFVDNRTIAAGDRLIRARRFVIATGSSPAIPPIPGLAETPFLT
NETLFSLKRLPRHLVVIGGGPVGLEMAGAHRRLGVEVTVVDKTEALSGQDPELAAIVLDGLRAEGVHLHERTAIHAVERT
ERGIRLRCENGNGPFGIEGSDLLVAAGRAPVHASLGLDAAGIRHDPKRIEVGPNLRTSNRRVYAIGDAAGGLFTHQASYH
ARLVLQQILFRLPAREKPFIVPRVIFTEPELAHVGLTEERAREAAPGATILRLDYSANDRSRTDGLGRGLIKVVVGRRGR
VLGAAIAGSGAGEMINLWAFAVANRLTLKHFQTYVAPYPTLSEIGKQAAISYYSPMARNRLLRTVLRFLRHFG

Specific function: Resistance to Hg(2+) in bacteria appears to be governed by a specialized system which includes mercuric reductase. MerA protein is responsible for volatilizing mercury as Hg(0) [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 HMA domains [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=468, Percent_Identity=30.1282051282051, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI50301238, Length=464, Percent_Identity=27.801724137931, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI148277065, Length=444, Percent_Identity=25.9009009009009, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI33519430, Length=444, Percent_Identity=25.9009009009009, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI33519428, Length=444, Percent_Identity=25.9009009009009, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI33519426, Length=444, Percent_Identity=25.9009009009009, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI148277071, Length=444, Percent_Identity=25.9009009009009, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI22035672, Length=428, Percent_Identity=27.803738317757, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI291045266, Length=431, Percent_Identity=27.1461716937355, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI291045268, Length=423, Percent_Identity=25.531914893617, Blast_Score=91, Evalue=3e-18,
Organism=Escherichia coli, GI1786307, Length=461, Percent_Identity=28.8503253796095, Blast_Score=161, Evalue=7e-41,
Organism=Escherichia coli, GI87081717, Length=448, Percent_Identity=28.5714285714286, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1789915, Length=430, Percent_Identity=30.2325581395349, Blast_Score=148, Evalue=9e-37,
Organism=Escherichia coli, GI87082354, Length=468, Percent_Identity=25.6410256410256, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1789065, Length=193, Percent_Identity=27.979274611399, Blast_Score=66, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI32565766, Length=477, Percent_Identity=31.0272536687631, Blast_Score=171, Evalue=8e-43,
Organism=Caenorhabditis elegans, GI17557007, Length=473, Percent_Identity=28.7526427061311, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71983419, Length=437, Percent_Identity=28.1464530892449, Blast_Score=121, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI71983429, Length=437, Percent_Identity=28.1464530892449, Blast_Score=121, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI71982272, Length=435, Percent_Identity=27.3563218390805, Blast_Score=96, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6321091, Length=479, Percent_Identity=29.4363256784969, Blast_Score=162, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6325166, Length=467, Percent_Identity=26.5524625267666, Blast_Score=113, Evalue=8e-26,
Organism=Saccharomyces cerevisiae, GI6325240, Length=469, Percent_Identity=26.4392324093817, Blast_Score=103, Evalue=8e-23,
Organism=Drosophila melanogaster, GI21358499, Length=463, Percent_Identity=30.4535637149028, Blast_Score=182, Evalue=6e-46,
Organism=Drosophila melanogaster, GI24640549, Length=471, Percent_Identity=30.9978768577495, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI24640553, Length=471, Percent_Identity=30.9978768577495, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI24640551, Length=471, Percent_Identity=30.9978768577495, Blast_Score=149, Evalue=4e-36,
Organism=Drosophila melanogaster, GI17737741, Length=464, Percent_Identity=29.5258620689655, Blast_Score=133, Evalue=2e-31,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR017969
- InterPro:   IPR006121
- InterPro:   IPR000815
- InterPro:   IPR021179
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00403 HMA; PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.16.1.1 [H]

Molecular weight: Translated: 50369; Mature: 50369

Theoretical pI: Translated: 10.18; Mature: 10.18

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKQLTPDICVIGGGAAGLTVAAGAAAFGVPVVLVEKGPMGGDCLNHGCVPSKALIAASR
CCCCCCCCEEEEECCCCCHHHHCCHHHHCCCEEEEECCCCCCHHHCCCCCCCHHHHHHHC
HAHSIRVAAEFGIAAAGPVIDQERLTARIQSVIVGIAPHDSVERFTSLGVEVIKDEACFV
CCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHEEECCCCHHHHHHHHCCHHEECCCEEEE
DNRTIAAGDRLIRARRFVIATGSSPAIPPIPGLAETPFLTNETLFSLKRLPRHLVVIGGG
CCCEEECCHHHEEEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCEEEEECCC
PVGLEMAGAHRRLGVEVTVVDKTEALSGQDPELAAIVLDGLRAEGVHLHERTAIHAVERT
CCCEEECCCCCEECEEEEEEECHHCCCCCCCCEEEEEEECCCCCCEEEHHHHHHHHHHHC
ERGIRLRCENGNGPFGIEGSDLLVAAGRAPVHASLGLDAAGIRHDPKRIEVGPNLRTSNR
CCCEEEEECCCCCCCCCCCCCEEEECCCCCEEEECCCCCCCCCCCCCEEEECCCCCCCCC
RVYAIGDAAGGLFTHQASYHARLVLQQILFRLPAREKPFIVPRVIFTEPELAHVGLTEER
EEEEEECCCCCEEEECCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCEECCCCHHH
AREAAPGATILRLDYSANDRSRTDGLGRGLIKVVVGRRGRVLGAAIAGSGAGEMINLWAF
HHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHH
AVANRLTLKHFQTYVAPYPTLSEIGKQAAISYYSPMARNRLLRTVLRFLRHFG
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVKQLTPDICVIGGGAAGLTVAAGAAAFGVPVVLVEKGPMGGDCLNHGCVPSKALIAASR
CCCCCCCCEEEEECCCCCHHHHCCHHHHCCCEEEEECCCCCCHHHCCCCCCCHHHHHHHC
HAHSIRVAAEFGIAAAGPVIDQERLTARIQSVIVGIAPHDSVERFTSLGVEVIKDEACFV
CCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHEEECCCCHHHHHHHHCCHHEECCCEEEE
DNRTIAAGDRLIRARRFVIATGSSPAIPPIPGLAETPFLTNETLFSLKRLPRHLVVIGGG
CCCEEECCHHHEEEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCEEEEECCC
PVGLEMAGAHRRLGVEVTVVDKTEALSGQDPELAAIVLDGLRAEGVHLHERTAIHAVERT
CCCEEECCCCCEECEEEEEEECHHCCCCCCCCEEEEEEECCCCCCEEEHHHHHHHHHHHC
ERGIRLRCENGNGPFGIEGSDLLVAAGRAPVHASLGLDAAGIRHDPKRIEVGPNLRTSNR
CCCEEEEECCCCCCCCCCCCCEEEECCCCCEEEECCCCCCCCCCCCCEEEECCCCCCCCC
RVYAIGDAAGGLFTHQASYHARLVLQQILFRLPAREKPFIVPRVIFTEPELAHVGLTEER
EEEEEECCCCCEEEECCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCEECCCCHHH
AREAAPGATILRLDYSANDRSRTDGLGRGLIKVVVGRRGRVLGAAIAGSGAGEMINLWAF
HHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHH
AVANRLTLKHFQTYVAPYPTLSEIGKQAAISYYSPMARNRLLRTVLRFLRHFG
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2536669; 10559175; 2067577 [H]