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The map label for this gene is zraS [H]

Identifier: 150395313

GI number: 150395313

Start: 93792

End: 95273

Strand: Direct

Name: zraS [H]

Synonym: Smed_0085

Alternate gene names: 150395313

Gene position: 93792-95273 (Clockwise)

Preceding gene: 150395312

Following gene: 150395314

Centisome position: 2.48

GC content: 64.1

Gene sequence:

>1482_bases
ATGGTGGAAGATGCGCGTCCGGCCGATCCGAGTGCGGCGCCACGAGCGGCGGCCGGCTATCTTGGTGGCCTTTCCGGCAA
ATTGCTGCTGCTGACGGTTGCCTTCGTCATGCTGGCCGAGGTGCTGATCTTCGTGCCCTCCGTCGCCAATATGCGCATTC
GCTGGCTGCAGGACCGCCTGAACACCGTGGCGGCGGCGGCTGTCGTCGTCGACGGCCTGCAGAATGTCGAGCTGCCCCGC
GCCGTTCAGCGCGAGACCCTGATGGCGACCGGCACGAAAGCCATCGTCATCCGCCGAAAGGATGCCTCGCGGATGATCGC
CAGCGTCGATATGCCCCCGGCCATCGACGGGGAGTACGACCTTGCCGACTTCACGGCCCTCGGCGCAGTCCGGGACGCTT
TCGACACGCTTCTTTTCGGGGGCCACCGGGTGGTGCGAATCTATGGGCCGCTAGGCGAGGGCGATGCGACGATCGAGCTG
GTGATGAAGGATGCCAAGCTGCGCACGGCCATGCTCGTCTATTCCCGCAACGTCTTCCTGCTGTCGATCGTCATATCGCT
GATCACCGCGGCCCTCATCTTCCTGGCAATCAATCGCATGCTGATCGTTCCGATACGACGGCTGACCAAAAGCATGCAGG
AGTTCTCGAACGAGCCGTCGAACCCCGAACGCGTGCTCGTGCCGCCGGACGGCAAGGATGAACTGGCGGTGGCCGGGCAG
CACCTTGCCAGCATGCAGCGCGAATTGCAGAGAACTTTGAAACAGCAGAAGAGCCTTGCCGAGCTCGGCCTTGCCGTTTC
CAAGATCAACCACGACATGCGCAACATCCTCTCTTCCGCGCAGCTCATTTCCGACCGCCTGGCCGCTGTCGACGATCCGG
TGGTCAAGCGCTTCGCACCGACATTGCTCAGGGCCATCGATCGCGCCGTCGGCTACACGCGCGAGGTTCTCTCCTACGGG
CGCACCACGGAGGCGGAGCCCCATCGCCGCTTCGTGTCGCTCCGGCCGCTTGTCGAGGACGTGGCCGAACTGCTTTCCGT
CGACCGGCAGGACGGCATCGATTTCCAGATACAGGTGCGAGAAGACATCGAGGTGGATGCGGATAGCGAGCAGCTCTTTC
GCGTCGTCCACAATATCTGCCGCAATGCCGTCGAGGCGCTTGCAAACTACACGCCCGAGAACGGTTCCGAGAGACGGATC
TCCGTTTCGGCGGTGCGGACCGGCAGCGTTGTTACGATTTCGATCGAGGACACCGGTCCTGGCATGCCGGCCAAAGCGCG
CGAAAATCTCTTCGCCGCCTTCCGGGGCTCGGCCCGATCGGGCGGCACGGGCCTGGGTCTCGCAATCGCACGCGAACTGG
TGCTCGCCCATGGCGGAACCATCGCCCTCGTCGAAAAGCCCACCCCCGGCACGCTGTTTCGCATAGAACTGCCGGACCGC
CCGGTACGGCTCGATGCCTTCCGCGCCAAGGGACGGTCCTGA

Upstream 100 bases:

>100_bases
GGCGATCTCCTATTATTCCCCGATGGCGCGAAATCGCCTTCTGCGGACCGTGCTTCGGTTCCTCAGACATTTCGGCTGAC
GGCAGGGAATATTGAGAGTC

Downstream 100 bases:

>100_bases
TGAGGTTCTCGTCCGCACCGGAGGAGACGCAGGGATACCGTCGCAAAAACAGAATTTTTGGCTAAAGGCGCTTGCAATCG
CCGAAAGGACCCTTTAGAGG

Product: integral membrane sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MVEDARPADPSAAPRAAAGYLGGLSGKLLLLTVAFVMLAEVLIFVPSVANMRIRWLQDRLNTVAAAAVVVDGLQNVELPR
AVQRETLMATGTKAIVIRRKDASRMIASVDMPPAIDGEYDLADFTALGAVRDAFDTLLFGGHRVVRIYGPLGEGDATIEL
VMKDAKLRTAMLVYSRNVFLLSIVISLITAALIFLAINRMLIVPIRRLTKSMQEFSNEPSNPERVLVPPDGKDELAVAGQ
HLASMQRELQRTLKQQKSLAELGLAVSKINHDMRNILSSAQLISDRLAAVDDPVVKRFAPTLLRAIDRAVGYTREVLSYG
RTTEAEPHRRFVSLRPLVEDVAELLSVDRQDGIDFQIQVREDIEVDADSEQLFRVVHNICRNAVEALANYTPENGSERRI
SVSAVRTGSVVTISIEDTGPGMPAKARENLFAAFRGSARSGGTGLGLAIARELVLAHGGTIALVEKPTPGTLFRIELPDR
PVRLDAFRAKGRS

Sequences:

>Translated_493_residues
MVEDARPADPSAAPRAAAGYLGGLSGKLLLLTVAFVMLAEVLIFVPSVANMRIRWLQDRLNTVAAAAVVVDGLQNVELPR
AVQRETLMATGTKAIVIRRKDASRMIASVDMPPAIDGEYDLADFTALGAVRDAFDTLLFGGHRVVRIYGPLGEGDATIEL
VMKDAKLRTAMLVYSRNVFLLSIVISLITAALIFLAINRMLIVPIRRLTKSMQEFSNEPSNPERVLVPPDGKDELAVAGQ
HLASMQRELQRTLKQQKSLAELGLAVSKINHDMRNILSSAQLISDRLAAVDDPVVKRFAPTLLRAIDRAVGYTREVLSYG
RTTEAEPHRRFVSLRPLVEDVAELLSVDRQDGIDFQIQVREDIEVDADSEQLFRVVHNICRNAVEALANYTPENGSERRI
SVSAVRTGSVVTISIEDTGPGMPAKARENLFAAFRGSARSGGTGLGLAIARELVLAHGGTIALVEKPTPGTLFRIELPDR
PVRLDAFRAKGRS
>Mature_493_residues
MVEDARPADPSAAPRAAAGYLGGLSGKLLLLTVAFVMLAEVLIFVPSVANMRIRWLQDRLNTVAAAAVVVDGLQNVELPR
AVQRETLMATGTKAIVIRRKDASRMIASVDMPPAIDGEYDLADFTALGAVRDAFDTLLFGGHRVVRIYGPLGEGDATIEL
VMKDAKLRTAMLVYSRNVFLLSIVISLITAALIFLAINRMLIVPIRRLTKSMQEFSNEPSNPERVLVPPDGKDELAVAGQ
HLASMQRELQRTLKQQKSLAELGLAVSKINHDMRNILSSAQLISDRLAAVDDPVVKRFAPTLLRAIDRAVGYTREVLSYG
RTTEAEPHRRFVSLRPLVEDVAELLSVDRQDGIDFQIQVREDIEVDADSEQLFRVVHNICRNAVEALANYTPENGSERRI
SVSAVRTGSVVTISIEDTGPGMPAKARENLFAAFRGSARSGGTGLGLAIARELVLAHGGTIALVEKPTPGTLFRIELPDR
PVRLDAFRAKGRS

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=246, Percent_Identity=30.0813008130081, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788549, Length=242, Percent_Identity=28.5123966942149, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1790300, Length=271, Percent_Identity=29.520295202952, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788393, Length=293, Percent_Identity=25.938566552901, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1789149, Length=192, Percent_Identity=29.1666666666667, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786912, Length=119, Percent_Identity=35.2941176470588, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1787374, Length=482, Percent_Identity=21.5767634854772, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87082128, Length=312, Percent_Identity=25, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 53750; Mature: 53750

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEDARPADPSAAPRAAAGYLGGLSGKLLLLTVAFVMLAEVLIFVPSVANMRIRWLQDRL
CCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
NTVAAAAVVVDGLQNVELPRAVQRETLMATGTKAIVIRRKDASRMIASVDMPPAIDGEYD
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEEECCHHHHHHCCCCCCCCCCCCC
LADFTALGAVRDAFDTLLFGGHRVVRIYGPLGEGDATIELVMKDAKLRTAMLVYSRNVFL
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHHCCHHH
LSIVISLITAALIFLAINRMLIVPIRRLTKSMQEFSNEPSNPERVLVPPDGKDELAVAGQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHH
HLASMQRELQRTLKQQKSLAELGLAVSKINHDMRNILSSAQLISDRLAAVDDPVVKRFAP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
TLLRAIDRAVGYTREVLSYGRTTEAEPHRRFVSLRPLVEDVAELLSVDRQDGIDFQIQVR
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
EDIEVDADSEQLFRVVHNICRNAVEALANYTPENGSERRISVSAVRTGSVVTISIEDTGP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCEEEEEEECCCC
GMPAKARENLFAAFRGSARSGGTGLGLAIARELVLAHGGTIALVEKPTPGTLFRIELPDR
CCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEECCCC
PVRLDAFRAKGRS
CEEEHHHHCCCCC
>Mature Secondary Structure
MVEDARPADPSAAPRAAAGYLGGLSGKLLLLTVAFVMLAEVLIFVPSVANMRIRWLQDRL
CCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
NTVAAAAVVVDGLQNVELPRAVQRETLMATGTKAIVIRRKDASRMIASVDMPPAIDGEYD
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEEECCHHHHHHCCCCCCCCCCCCC
LADFTALGAVRDAFDTLLFGGHRVVRIYGPLGEGDATIELVMKDAKLRTAMLVYSRNVFL
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHHCCHHH
LSIVISLITAALIFLAINRMLIVPIRRLTKSMQEFSNEPSNPERVLVPPDGKDELAVAGQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHH
HLASMQRELQRTLKQQKSLAELGLAVSKINHDMRNILSSAQLISDRLAAVDDPVVKRFAP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
TLLRAIDRAVGYTREVLSYGRTTEAEPHRRFVSLRPLVEDVAELLSVDRQDGIDFQIQVR
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
EDIEVDADSEQLFRVVHNICRNAVEALANYTPENGSERRISVSAVRTGSVVTISIEDTGP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCEEEEEEECCCC
GMPAKARENLFAAFRGSARSGGTGLGLAIARELVLAHGGTIALVEKPTPGTLFRIELPDR
CCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEECCCC
PVRLDAFRAKGRS
CEEEHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 2666400; 11243806 [H]