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The map label for this gene is dapF

Identifier: 150400642

GI number: 150400642

Start: 217494

End: 218381

Strand: Direct

Name: dapF

Synonym: Maeo_0204

Alternate gene names: 150400642

Gene position: 217494-218381 (Clockwise)

Preceding gene: 150400641

Following gene: 150400645

Centisome position: 13.86

GC content: 32.21

Gene sequence:

>888_bases
ATGGGTTTAAACTTTACAAAAATGCACGGACTTGGAAACGATTATATCGTAATAAATGAATTCGATGGAGAAAAAATAAA
GGAAGACGAAAAGGCAGAATTTTCTAAAAAAATCTGTAAAAGGGGATTTTCAATAGGTGCCGATGGTGTAATATTTGTCC
AACCTGCAACCGATGAAAAATATGATATAAGATTTAGAATTTTTAACAGCGACGGGTCAGAAGCTGAGATGTGCGGAAAT
GGAATAAGATGCTTCTCAAAATTTGTTTATGAAAGAGTAATGCAAAAAAATCCATTAAATGTTGAAACCATGGGCGGTCT
TAGGGTTTCAGAAATGAATATAGAAAATAATATTGTAAAATCTATTAAAGTATTTATGGGAGCTCCCACATTTGAACTAA
AAGACATACCTATGGTAGTAGAAGGAAAGTCAGAAAACGATGTATTTTTAGACGAAGAAATTGAATTAAAAAATGCACTA
TTAAATTCTGTAAAATTAAGTGTTGTGAATGTTGGAAATCCTCATGCGGTAATATTTATGAAGGACAATAATTTAAAGGC
ACCATTCATAAGAACCCACTTAAATATATTGGGTAAAGAAATAGAGCATCATAGTGTATTTCCAGAAAAAATAAATGTTC
ATTTTGTAGAGATAGTAAATCCACAGGAGATAAAAATAATTACTTGGGAAAGGGGGGCAGGTTATACCACAGCTTGTGGA
ACAGGGACAACTAGCTCTGTAATAATTGCCAACAAATTAGGAAAAACAGGAAATAAAGTTTTGGCACATTTAGACGGTGG
AGATTTAGAAATTGAAATTAAAGAAGATGGAGTTTATATGGAAGGGGATGCCGTAATTGTTTATGACGGGGAATTATATC
AATATTAA

Upstream 100 bases:

>100_bases
TCTTGAATATATGTCACACATCACACATATAATTCATTAATCAATATATAGTGTTTTTAAATTTGTCGGTCTAAAATATT
AAAATAAAAGGTGAAATAAT

Downstream 100 bases:

>100_bases
AAATAATTATAAATATTAATGGATTATTTTTTAATTATTATTTTTTTGGTTTTGATTTTATTTGAAATAAAGACTTATAT
GGATAGCAATAACTCCCATA

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MGLNFTKMHGLGNDYIVINEFDGEKIKEDEKAEFSKKICKRGFSIGADGVIFVQPATDEKYDIRFRIFNSDGSEAEMCGN
GIRCFSKFVYERVMQKNPLNVETMGGLRVSEMNIENNIVKSIKVFMGAPTFELKDIPMVVEGKSENDVFLDEEIELKNAL
LNSVKLSVVNVGNPHAVIFMKDNNLKAPFIRTHLNILGKEIEHHSVFPEKINVHFVEIVNPQEIKIITWERGAGYTTACG
TGTTSSVIIANKLGKTGNKVLAHLDGGDLEIEIKEDGVYMEGDAVIVYDGELYQY

Sequences:

>Translated_295_residues
MGLNFTKMHGLGNDYIVINEFDGEKIKEDEKAEFSKKICKRGFSIGADGVIFVQPATDEKYDIRFRIFNSDGSEAEMCGN
GIRCFSKFVYERVMQKNPLNVETMGGLRVSEMNIENNIVKSIKVFMGAPTFELKDIPMVVEGKSENDVFLDEEIELKNAL
LNSVKLSVVNVGNPHAVIFMKDNNLKAPFIRTHLNILGKEIEHHSVFPEKINVHFVEIVNPQEIKIITWERGAGYTTACG
TGTTSSVIIANKLGKTGNKVLAHLDGGDLEIEIKEDGVYMEGDAVIVYDGELYQY
>Mature_294_residues
GLNFTKMHGLGNDYIVINEFDGEKIKEDEKAEFSKKICKRGFSIGADGVIFVQPATDEKYDIRFRIFNSDGSEAEMCGNG
IRCFSKFVYERVMQKNPLNVETMGGLRVSEMNIENNIVKSIKVFMGAPTFELKDIPMVVEGKSENDVFLDEEIELKNALL
NSVKLSVVNVGNPHAVIFMKDNNLKAPFIRTHLNILGKEIEHHSVFPEKINVHFVEIVNPQEIKIITWERGAGYTTACGT
GTTSSVIIANKLGKTGNKVLAHLDGGDLEIEIKEDGVYMEGDAVIVYDGELYQY

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=294, Percent_Identity=33.6734693877551, Blast_Score=169, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_META3 (A6UTH4)

Other databases:

- EMBL:   CP000743
- RefSeq:   YP_001324408.1
- ProteinModelPortal:   A6UTH4
- SMR:   A6UTH4
- STRING:   A6UTH4
- GeneID:   5326820
- GenomeReviews:   CP000743_GR
- KEGG:   mae:Maeo_0204
- eggNOG:   arNOG07445
- HOGENOM:   HBG399442
- OMA:   HTGIGFD
- ProtClustDB:   PRK00450
- BioCyc:   MAEO419665:MAEO_0204-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 32981; Mature: 32850

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 78-78 ACT_SITE 239-239

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLNFTKMHGLGNDYIVINEFDGEKIKEDEKAEFSKKICKRGFSIGADGVIFVQPATDEK
CCCCEEEECCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCC
YDIRFRIFNSDGSEAEMCGNGIRCFSKFVYERVMQKNPLNVETMGGLRVSEMNIENNIVK
EEEEEEEECCCCCHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEECCEEEEEECCCHHHHH
SIKVFMGAPTFELKDIPMVVEGKSENDVFLDEEIELKNALLNSVKLSVVNVGNPHAVIFM
HHHHHHCCCCEEECCCCEEEECCCCCCEEECCCHHHHHHHHCCEEEEEEECCCCEEEEEE
KDNNLKAPFIRTHLNILGKEIEHHSVFPEKINVHFVEIVNPQEIKIITWERGAGYTTACG
ECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCEEEEEEEECCCCEEEECC
TGTTSSVIIANKLGKTGNKVLAHLDGGDLEIEIKEDGVYMEGDAVIVYDGELYQY
CCCCCEEEEEECCCCCCCEEEEEECCCCEEEEEECCCEEEECCEEEEECCCEECC
>Mature Secondary Structure 
GLNFTKMHGLGNDYIVINEFDGEKIKEDEKAEFSKKICKRGFSIGADGVIFVQPATDEK
CCCEEEECCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCC
YDIRFRIFNSDGSEAEMCGNGIRCFSKFVYERVMQKNPLNVETMGGLRVSEMNIENNIVK
EEEEEEEECCCCCHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEECCEEEEEECCCHHHHH
SIKVFMGAPTFELKDIPMVVEGKSENDVFLDEEIELKNALLNSVKLSVVNVGNPHAVIFM
HHHHHHCCCCEEECCCCEEEECCCCCCEEECCCHHHHHHHHCCEEEEEEECCCCEEEEEE
KDNNLKAPFIRTHLNILGKEIEHHSVFPEKINVHFVEIVNPQEIKIITWERGAGYTTACG
ECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCEEEEEEEECCCCEEEECC
TGTTSSVIIANKLGKTGNKVLAHLDGGDLEIEIKEDGVYMEGDAVIVYDGELYQY
CCCCCEEEEEECCCCCCCEEEEEECCCCEEEEEECCCEEEECCEEEEECCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA