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The map label for this gene is carB1 [H]

Identifier: 150400482

GI number: 150400482

Start: 48701

End: 50140

Strand: Direct

Name: carB1 [H]

Synonym: Maeo_0044

Alternate gene names: 150400482

Gene position: 48701-50140 (Clockwise)

Preceding gene: 150400481

Following gene: 150400483

Centisome position: 3.1

GC content: 35.56

Gene sequence:

>1440_bases
TTGAAACAGGACAACATAAAAAAGGTTATGATTTTAGGTTCTGGTCCTATCGTAATAGGGCAGGCGGCAGAGTTTGATTT
TTCTGGTTCTCAGGCATGTAAATCTTTGAGAGAGGAAGGGATATACACCATTTTAGTAAATTCAAATCCTGCAACAATTC
AAACTGATAAGGATATTGCAGACAAGGTTTATCTTGAACCATTGAACCCAAAAATAGTGGAGAAAATTATAGAAAAAGAA
AGACCTGATGCAATACTACCTACAATGGGCGGGCAGACAGGTTTAAATCTTGCCGTAGATTTGCATAGAATGGGAATATT
GGAAAAATATGGAGTAAAATTACTTGGTTCAACTGTTGATGTGATTGAAACTAGTGAAGATAGAGAATTATTTAATAAAG
CAATGGATGAAATTGGAGAGCCTGTCACAAAATCTTATGCAGTTGAATCAATTGAGAAAGCTCTTGAAGCTGTGGAAGAA
TTGGGCTACCCCGTAATAGTTAGACCCGCCTTTACACTTGGCGGAACTGGTGGGGGTATTCCATATACAGAAGAAGAATT
AAGAGATATCGTAGAAAAAGGACTTAACTATTCTATGATTAACCAAGTTTTGATTGACGAAAGTGTGCTTGGCTGGAAGG
AGTATGAATATGAAGTAATGAGAGACGCAAAAGATAACTGTATAATTGTATGTAATATGGAAAATATCGACCCTATGGGA
ATACATACAGGAGAAAGTATTGTTGTAGCGCCATCTCAAACTCTTAGCGATAAAATGCACCAAAGGCTTAGAAATGCGGC
TTTAAAAATAATTAGGCACTTAAACATTATCGGTGGCTGTAATGTTCAGTTTTCAGTGAGCCCAGATGATAGCGAATATA
GAGTAATTGAGGTAAATCCGCGAGTTAGTAGGAGCTCCGCATTAGCAAGTAAGGCAACAGGTTATTCAATTGCTAAAATT
GCAGCTAAAATTGCCATCGGTAAAACACTTAATGAGATTAAAAATGATATTACAGCAGAAACCACGGCCGCATTTGAGCC
GACTTTGGATTATGTGATTGTAAAAATACCAAGATGGCCATTTGATAAATTTAAAACTGTTGATAAACATCTTGGAACAA
GCATGAAATCAACAGGGGAAGTTATGGCAATAGGTAGGACATTAGAGGAAGCACTTCAAAAAGCATGCAGGAGCCTTGAT
TTGGGGAGACAAGGTTTAATTGGAGATGGCAATGATATATCCCATACCAACGAAGAAATTAACGATATATTAAAAAATCC
GACCGATGAAAGAATATTTGTAATAGCTGAGGCACTTGATAAGGGCTGGACAGTTGAGGAAATTTGTGAAATTACAAAAA
TTAATGAATTTTTCATTAAAAAAATAAAAAATATTGTAGATACCAAAAAAGAATTGGAAATATTATCTAAATTGATTTAA

Upstream 100 bases:

>100_bases
TTTTATATGATTTACCCATATAATATTTATATATAATTCTGATGAAAACTTTTTTTTATACAATTTATCTTATACAATTA
TTAAGTTAATAGGTGGAATT

Downstream 100 bases:

>100_bases
TTATTAAGTTAATTATTAGTATTAAGAATATTGGTATTACTAATTATTAACATTACTATATTTTATTATATTTTTATTAT
TAGCATGTTTGTCGGTGAAT

Product: carbamoyl-phosphate synthase L chain ATP-binding

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase ammonia chain [H]

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MKQDNIKKVMILGSGPIVIGQAAEFDFSGSQACKSLREEGIYTILVNSNPATIQTDKDIADKVYLEPLNPKIVEKIIEKE
RPDAILPTMGGQTGLNLAVDLHRMGILEKYGVKLLGSTVDVIETSEDRELFNKAMDEIGEPVTKSYAVESIEKALEAVEE
LGYPVIVRPAFTLGGTGGGIPYTEEELRDIVEKGLNYSMINQVLIDESVLGWKEYEYEVMRDAKDNCIIVCNMENIDPMG
IHTGESIVVAPSQTLSDKMHQRLRNAALKIIRHLNIIGGCNVQFSVSPDDSEYRVIEVNPRVSRSSALASKATGYSIAKI
AAKIAIGKTLNEIKNDITAETTAAFEPTLDYVIVKIPRWPFDKFKTVDKHLGTSMKSTGEVMAIGRTLEEALQKACRSLD
LGRQGLIGDGNDISHTNEEINDILKNPTDERIFVIAEALDKGWTVEEICEITKINEFFIKKIKNIVDTKKELEILSKLI

Sequences:

>Translated_479_residues
MKQDNIKKVMILGSGPIVIGQAAEFDFSGSQACKSLREEGIYTILVNSNPATIQTDKDIADKVYLEPLNPKIVEKIIEKE
RPDAILPTMGGQTGLNLAVDLHRMGILEKYGVKLLGSTVDVIETSEDRELFNKAMDEIGEPVTKSYAVESIEKALEAVEE
LGYPVIVRPAFTLGGTGGGIPYTEEELRDIVEKGLNYSMINQVLIDESVLGWKEYEYEVMRDAKDNCIIVCNMENIDPMG
IHTGESIVVAPSQTLSDKMHQRLRNAALKIIRHLNIIGGCNVQFSVSPDDSEYRVIEVNPRVSRSSALASKATGYSIAKI
AAKIAIGKTLNEIKNDITAETTAAFEPTLDYVIVKIPRWPFDKFKTVDKHLGTSMKSTGEVMAIGRTLEEALQKACRSLD
LGRQGLIGDGNDISHTNEEINDILKNPTDERIFVIAEALDKGWTVEEICEITKINEFFIKKIKNIVDTKKELEILSKLI
>Mature_479_residues
MKQDNIKKVMILGSGPIVIGQAAEFDFSGSQACKSLREEGIYTILVNSNPATIQTDKDIADKVYLEPLNPKIVEKIIEKE
RPDAILPTMGGQTGLNLAVDLHRMGILEKYGVKLLGSTVDVIETSEDRELFNKAMDEIGEPVTKSYAVESIEKALEAVEE
LGYPVIVRPAFTLGGTGGGIPYTEEELRDIVEKGLNYSMINQVLIDESVLGWKEYEYEVMRDAKDNCIIVCNMENIDPMG
IHTGESIVVAPSQTLSDKMHQRLRNAALKIIRHLNIIGGCNVQFSVSPDDSEYRVIEVNPRVSRSSALASKATGYSIAKI
AAKIAIGKTLNEIKNDITAETTAAFEPTLDYVIVKIPRWPFDKFKTVDKHLGTSMKSTGEVMAIGRTLEEALQKACRSLD
LGRQGLIGDGNDISHTNEEINDILKNPTDERIFVIAEALDKGWTVEEICEITKINEFFIKKIKNIVDTKKELEILSKLI

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0458

COG function: function code EF; Carbamoylphosphate synthase large subunit (split gene in MJ)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=468, Percent_Identity=52.7777777777778, Blast_Score=506, Evalue=1e-143,
Organism=Homo sapiens, GI21361331, Length=476, Percent_Identity=49.5798319327731, Blast_Score=489, Evalue=1e-138,
Organism=Homo sapiens, GI169790915, Length=476, Percent_Identity=49.5798319327731, Blast_Score=488, Evalue=1e-138,
Organism=Homo sapiens, GI170295797, Length=446, Percent_Identity=48.4304932735426, Blast_Score=447, Evalue=1e-126,
Organism=Escherichia coli, GI1786216, Length=472, Percent_Identity=53.3898305084746, Blast_Score=523, Evalue=1e-149,
Organism=Caenorhabditis elegans, GI193204318, Length=468, Percent_Identity=53.4188034188034, Blast_Score=508, Evalue=1e-144,
Organism=Saccharomyces cerevisiae, GI6322569, Length=476, Percent_Identity=52.1008403361345, Blast_Score=507, Evalue=1e-144,
Organism=Saccharomyces cerevisiae, GI6322331, Length=461, Percent_Identity=54.0130151843818, Blast_Score=498, Evalue=1e-141,
Organism=Drosophila melanogaster, GI45555749, Length=460, Percent_Identity=53.9130434782609, Blast_Score=517, Evalue=1e-147,
Organism=Drosophila melanogaster, GI24642586, Length=460, Percent_Identity=53.9130434782609, Blast_Score=517, Evalue=1e-147,

Paralogues:

None

Copy number: 4701 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR005479
- InterPro:   IPR005483
- InterPro:   IPR005481
- InterPro:   IPR005480
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF00289 CPSase_L_chain; PF02786 CPSase_L_D2; PF02787 CPSase_L_D3 [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 53047; Mature: 53047

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS50975 ATP_GRASP ; PS00866 CPSASE_1 ; PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQDNIKKVMILGSGPIVIGQAAEFDFSGSQACKSLREEGIYTILVNSNPATIQTDKDIA
CCCCCCEEEEEEECCCEEEECCCCCCCCHHHHHHHHHHCCEEEEEECCCCCEEECCCHHH
DKVYLEPLNPKIVEKIIEKERPDAILPTMGGQTGLNLAVDLHRMGILEKYGVKLLGSTVD
HHHEECCCCHHHHHHHHHHCCCCEECCCCCCCCCCEEEEHHHHHHHHHHHCHHHHCCCHH
VIETSEDRELFNKAMDEIGEPVTKSYAVESIEKALEAVEELGYPVIVRPAFTLGGTGGGI
HHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCEEECCCCCCC
PYTEEELRDIVEKGLNYSMINQVLIDESVLGWKEYEYEVMRDAKDNCIIVCNMENIDPMG
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEEEECCCCCCCC
IHTGESIVVAPSQTLSDKMHQRLRNAALKIIRHLNIIGGCNVQFSVSPDDSEYRVIEVNP
CCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEECCCCCCEEEEEECC
RVSRSSALASKATGYSIAKIAAKIAIGKTLNEIKNDITAETTAAFEPTLDYVIVKIPRWP
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCEEEEECCCCC
FDKFKTVDKHLGTSMKSTGEVMAIGRTLEEALQKACRSLDLGRQGLIGDGNDISHTNEEI
CHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHH
NDILKNPTDERIFVIAEALDKGWTVEEICEITKINEFFIKKIKNIVDTKKELEILSKLI
HHHHCCCCCCEEEEEEEHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure
MKQDNIKKVMILGSGPIVIGQAAEFDFSGSQACKSLREEGIYTILVNSNPATIQTDKDIA
CCCCCCEEEEEEECCCEEEECCCCCCCCHHHHHHHHHHCCEEEEEECCCCCEEECCCHHH
DKVYLEPLNPKIVEKIIEKERPDAILPTMGGQTGLNLAVDLHRMGILEKYGVKLLGSTVD
HHHEECCCCHHHHHHHHHHCCCCEECCCCCCCCCCEEEEHHHHHHHHHHHCHHHHCCCHH
VIETSEDRELFNKAMDEIGEPVTKSYAVESIEKALEAVEELGYPVIVRPAFTLGGTGGGI
HHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCEEECCCCCCC
PYTEEELRDIVEKGLNYSMINQVLIDESVLGWKEYEYEVMRDAKDNCIIVCNMENIDPMG
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEEEECCCCCCCC
IHTGESIVVAPSQTLSDKMHQRLRNAALKIIRHLNIIGGCNVQFSVSPDDSEYRVIEVNP
CCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEECCCCCCEEEEEECC
RVSRSSALASKATGYSIAKIAAKIAIGKTLNEIKNDITAETTAAFEPTLDYVIVKIPRWP
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCEEEEECCCCC
FDKFKTVDKHLGTSMKSTGEVMAIGRTLEEALQKACRSLDLGRQGLIGDGNDISHTNEEI
CHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHH
NDILKNPTDERIFVIAEALDKGWTVEEICEITKINEFFIKKIKNIVDTKKELEILSKLI
HHHHCCCCCCEEEEEEEHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]