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The map label for this gene is carB2 [H]

Identifier: 150400483

GI number: 150400483

Start: 50244

End: 52058

Strand: Direct

Name: carB2 [H]

Synonym: Maeo_0045

Alternate gene names: 150400483

Gene position: 50244-52058 (Clockwise)

Preceding gene: 150400482

Following gene: 150400484

Centisome position: 3.2

GC content: 34.05

Gene sequence:

>1815_bases
ATGATGGAAACAGAAAAACTAAAAGAAATAATATTTAATGCCAAAAAAATGGGTTTTTCAGATGCCCAGTTATCTAATTT
ATTGAATAAAAGCGAATCAGAGATAAAAAACCTTAGAAAAGAATTAAATGTAATGCCTGTTTTTAAAATGGTGGATACCT
GCGCAGCAGAATTTGAGGCAAAAACTCCATATTATTATTCTTGCTACGAAAATTTATTTTATGATGAACAAAATGAAAGC
ATACCAACAAAAAGAAAAAAGGTAATAATATTGGGTTCTGGACCTATCAGAATTGGACAAGGTGTAGAATTTGATTATTC
CACCGTTCATGCCATATATGCTTTAAAAGATATGGGTATTGAAGCAATAATTGTAAATAACAATCCTGAAACTGTATCCA
CTGATTACGATACCTCCGATAAATTATATTTTGAGCCATTAACCTATGAGGAAATTATGAATATCGTTGAAAATGAAAAT
AAAAATGGCGATTTATTGGGAGTAATAGTTCAATTTGGAGGGCAGACTGCAATAAACCTTGCTATGAGATTATATGATTC
TGGTGTAAATATATTGGGAACTTCTCCAAAATCTATTGATTTAGCTGAGGATAGGGAGCAATTTTCAGAGATATTAAATA
AAATAGGTATTCCACAGGCTGAGGGTGCTACTGTATATAATGAAAAAGATGCACTTGTTGTAGCTAAAAAAATTGGATAT
CCTGTTCTTGTTCGACCTTCCTATGTTTTGGGGGGTAGGGCTATGGAAATTGTATATAATGAAGAGGAATTAAAGAACTA
CATGAAAGAAGCCGTAAAAGTATCATCTGAACATCCCATATTAATCGATAAGTTTTTAGATGAGGCTACGGAGGTTGATG
TAGATGCTATTTGCGATGGGGACGATGTATTTATTGGGGGAATTATGGAGCATATCGAGGAAGCTGGAATTCACAGTGGA
GATAGTGCCTGTGTAATACCATCTCAAACTTTATCTCCACAAGTAATAGATACCATTATTGACCATACCACCAATCTTGC
AAAGGAATTGGGTGTAATTGGACTGTTGAATATACAGTATGCCGTTAAAGATGGTGTAGTTTATATTATTGAAGCAAATC
CACGGGCAAGTAGGACCATACCATATGTAAGTAAATCTGTGGGAGCTCCCCTTGCCAAAATTGCTACAAAGGTAATCATG
GGGCAAAAATTAAAAGATATGGGATATATTGGATTAGCTCAATCAAAATATGTGAGCATTAAAGAAGCCGTATTCCCATT
CCAAAAGCTAACCGGAGTAGACCCAGTGCTTAGTCCAGAAATGAAATCCACAGGAGAGGCTATTGGAATAGATACAGATT
TTGGTAGGGCATTTTATAAAGCTCAACTGTCTGCCAACATGGAGCTCCCAATGTCTGGAACTGTGTTCATAAGTGTTAAG
AAAAAGGATAGAAAACATTTAGAAAAAATGGCAAAAGAATTTAATAAATTTGGATTTAATATTATTGCAACAGATGGAAC
AGCTGAGGAAATTAGAAGTAATGGAGTAGATGCTACAATAGTCAAAAAAATATCCGAAGGTATAAAAGGTAGCATATTGG
AAGAAATACAAAAAGGAAATGTTGATTTAATCATAAATACCTCTTCTGGAAGTAGGGCAAAATCTGATGGATACTACATA
AGACGAGCCGCCGTTGAATTTAATATACCATATATTACAACAATACAGGGAGCACTATCCACTATAAAGGCAATAAAATC
TGTTAAAGAAGGAGATTTGGGGGTTTATTCTTTAAATGACCTTGAAAATAAATAA

Upstream 100 bases:

>100_bases
TTAAGTTAATTATTAGTATTAAGAATATTGGTATTACTAATTATTAACATTACTATATTTTATTATATTTTTATTATTAG
CATGTTTGTCGGTGAATAAT

Downstream 100 bases:

>100_bases
TAAATATAATAGTAAAATACTAGTGATACAATGGAAAAATACTTAAAAGAATTAAGAAATAGGCACCTAAAAAAAAGAAA
AGAAAAAAATCCTGACCGCC

Product: carbamoyl-phosphate synthase L chain ATP-binding

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase ammonia chain [H]

Number of amino acids: Translated: 604; Mature: 604

Protein sequence:

>604_residues
MMETEKLKEIIFNAKKMGFSDAQLSNLLNKSESEIKNLRKELNVMPVFKMVDTCAAEFEAKTPYYYSCYENLFYDEQNES
IPTKRKKVIILGSGPIRIGQGVEFDYSTVHAIYALKDMGIEAIIVNNNPETVSTDYDTSDKLYFEPLTYEEIMNIVENEN
KNGDLLGVIVQFGGQTAINLAMRLYDSGVNILGTSPKSIDLAEDREQFSEILNKIGIPQAEGATVYNEKDALVVAKKIGY
PVLVRPSYVLGGRAMEIVYNEEELKNYMKEAVKVSSEHPILIDKFLDEATEVDVDAICDGDDVFIGGIMEHIEEAGIHSG
DSACVIPSQTLSPQVIDTIIDHTTNLAKELGVIGLLNIQYAVKDGVVYIIEANPRASRTIPYVSKSVGAPLAKIATKVIM
GQKLKDMGYIGLAQSKYVSIKEAVFPFQKLTGVDPVLSPEMKSTGEAIGIDTDFGRAFYKAQLSANMELPMSGTVFISVK
KKDRKHLEKMAKEFNKFGFNIIATDGTAEEIRSNGVDATIVKKISEGIKGSILEEIQKGNVDLIINTSSGSRAKSDGYYI
RRAAVEFNIPYITTIQGALSTIKAIKSVKEGDLGVYSLNDLENK

Sequences:

>Translated_604_residues
MMETEKLKEIIFNAKKMGFSDAQLSNLLNKSESEIKNLRKELNVMPVFKMVDTCAAEFEAKTPYYYSCYENLFYDEQNES
IPTKRKKVIILGSGPIRIGQGVEFDYSTVHAIYALKDMGIEAIIVNNNPETVSTDYDTSDKLYFEPLTYEEIMNIVENEN
KNGDLLGVIVQFGGQTAINLAMRLYDSGVNILGTSPKSIDLAEDREQFSEILNKIGIPQAEGATVYNEKDALVVAKKIGY
PVLVRPSYVLGGRAMEIVYNEEELKNYMKEAVKVSSEHPILIDKFLDEATEVDVDAICDGDDVFIGGIMEHIEEAGIHSG
DSACVIPSQTLSPQVIDTIIDHTTNLAKELGVIGLLNIQYAVKDGVVYIIEANPRASRTIPYVSKSVGAPLAKIATKVIM
GQKLKDMGYIGLAQSKYVSIKEAVFPFQKLTGVDPVLSPEMKSTGEAIGIDTDFGRAFYKAQLSANMELPMSGTVFISVK
KKDRKHLEKMAKEFNKFGFNIIATDGTAEEIRSNGVDATIVKKISEGIKGSILEEIQKGNVDLIINTSSGSRAKSDGYYI
RRAAVEFNIPYITTIQGALSTIKAIKSVKEGDLGVYSLNDLENK
>Mature_604_residues
MMETEKLKEIIFNAKKMGFSDAQLSNLLNKSESEIKNLRKELNVMPVFKMVDTCAAEFEAKTPYYYSCYENLFYDEQNES
IPTKRKKVIILGSGPIRIGQGVEFDYSTVHAIYALKDMGIEAIIVNNNPETVSTDYDTSDKLYFEPLTYEEIMNIVENEN
KNGDLLGVIVQFGGQTAINLAMRLYDSGVNILGTSPKSIDLAEDREQFSEILNKIGIPQAEGATVYNEKDALVVAKKIGY
PVLVRPSYVLGGRAMEIVYNEEELKNYMKEAVKVSSEHPILIDKFLDEATEVDVDAICDGDDVFIGGIMEHIEEAGIHSG
DSACVIPSQTLSPQVIDTIIDHTTNLAKELGVIGLLNIQYAVKDGVVYIIEANPRASRTIPYVSKSVGAPLAKIATKVIM
GQKLKDMGYIGLAQSKYVSIKEAVFPFQKLTGVDPVLSPEMKSTGEAIGIDTDFGRAFYKAQLSANMELPMSGTVFISVK
KKDRKHLEKMAKEFNKFGFNIIATDGTAEEIRSNGVDATIVKKISEGIKGSILEEIQKGNVDLIINTSSGSRAKSDGYYI
RRAAVEFNIPYITTIQGALSTIKAIKSVKEGDLGVYSLNDLENK

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0458

COG function: function code EF; Carbamoylphosphate synthase large subunit (split gene in MJ)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI170295797, Length=578, Percent_Identity=38.9273356401384, Blast_Score=419, Evalue=1e-117,
Organism=Homo sapiens, GI169790915, Length=578, Percent_Identity=38.9273356401384, Blast_Score=418, Evalue=1e-117,
Organism=Homo sapiens, GI21361331, Length=578, Percent_Identity=38.9273356401384, Blast_Score=418, Evalue=1e-117,
Organism=Homo sapiens, GI18105007, Length=594, Percent_Identity=37.7104377104377, Blast_Score=400, Evalue=1e-111,
Organism=Homo sapiens, GI106049528, Length=447, Percent_Identity=22.8187919463087, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI106049295, Length=447, Percent_Identity=22.8187919463087, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI106049292, Length=447, Percent_Identity=22.8187919463087, Blast_Score=72, Evalue=2e-12,
Organism=Escherichia coli, GI1786216, Length=574, Percent_Identity=50, Blast_Score=574, Evalue=1e-165,
Organism=Caenorhabditis elegans, GI193204318, Length=605, Percent_Identity=35.8677685950413, Blast_Score=358, Evalue=5e-99,
Organism=Saccharomyces cerevisiae, GI6322331, Length=591, Percent_Identity=38.0710659898477, Blast_Score=413, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6322569, Length=599, Percent_Identity=38.2303839732888, Blast_Score=387, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24642586, Length=593, Percent_Identity=35.0758853288364, Blast_Score=367, Evalue=1e-101,
Organism=Drosophila melanogaster, GI45555749, Length=517, Percent_Identity=36.3636363636364, Blast_Score=350, Evalue=2e-96,

Paralogues:

None

Copy number: 4701 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR005479
- InterPro:   IPR005483
- InterPro:   IPR005481
- InterPro:   IPR005480
- InterPro:   IPR011607
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF00289 CPSase_L_chain; PF02786 CPSase_L_D2; PF02787 CPSase_L_D3; PF02142 MGS [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 66602; Mature: 66602

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS50975 ATP_GRASP ; PS00866 CPSASE_1 ; PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMETEKLKEIIFNAKKMGFSDAQLSNLLNKSESEIKNLRKELNVMPVFKMVDTCAAEFEA
CCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
KTPYYYSCYENLFYDEQNESIPTKRKKVIILGSGPIRIGQGVEFDYSTVHAIYALKDMGI
CCCEEEEHHHHHHCCCCCCCCCCCCCEEEEECCCCEEECCCCCCCHHHHHHHHHHHHCCE
EAIIVNNNPETVSTDYDTSDKLYFEPLTYEEIMNIVENENKNGDLLGVIVQFGGQTAINL
EEEEECCCCCEEECCCCCCCCEEECCCCHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHH
AMRLYDSGVNILGTSPKSIDLAEDREQFSEILNKIGIPQAEGATVYNEKDALVVAKKIGY
HHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEECCCCCEEEEHHCCC
PVLVRPSYVLGGRAMEIVYNEEELKNYMKEAVKVSSEHPILIDKFLDEATEVDVDAICDG
CEEECCCEEECCEEEEEEECHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHCCCHHEEECC
DDVFIGGIMEHIEEAGIHSGDSACVIPSQTLSPQVIDTIIDHTTNLAKELGVIGLLNIQY
CCEEEHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEE
AVKDGVVYIIEANPRASRTIPYVSKSVGAPLAKIATKVIMGQKLKDMGYIGLAQSKYVSI
EECCCEEEEEECCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEECCCHHHHH
KEAVFPFQKLTGVDPVLSPEMKSTGEAIGIDTDFGRAFYKAQLSANMELPMSGTVFISVK
HHHHCCHHHHCCCCCCCCCCHHCCCCEEECCCHHHHHHHHHHHCCCCCCCCCCEEEEEEC
KKDRKHLEKMAKEFNKFGFNIIATDGTAEEIRSNGVDATIVKKISEGIKGSILEEIQKGN
CHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHCCC
VDLIINTSSGSRAKSDGYYIRRAAVEFNIPYITTIQGALSTIKAIKSVKEGDLGVYSLND
EEEEEECCCCCCCCCCCEEEEEEEEEECCCEEEHHHHHHHHHHHHHHHCCCCCEEEECCC
LENK
CCCC
>Mature Secondary Structure
MMETEKLKEIIFNAKKMGFSDAQLSNLLNKSESEIKNLRKELNVMPVFKMVDTCAAEFEA
CCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
KTPYYYSCYENLFYDEQNESIPTKRKKVIILGSGPIRIGQGVEFDYSTVHAIYALKDMGI
CCCEEEEHHHHHHCCCCCCCCCCCCCEEEEECCCCEEECCCCCCCHHHHHHHHHHHHCCE
EAIIVNNNPETVSTDYDTSDKLYFEPLTYEEIMNIVENENKNGDLLGVIVQFGGQTAINL
EEEEECCCCCEEECCCCCCCCEEECCCCHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHH
AMRLYDSGVNILGTSPKSIDLAEDREQFSEILNKIGIPQAEGATVYNEKDALVVAKKIGY
HHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEECCCCCEEEEHHCCC
PVLVRPSYVLGGRAMEIVYNEEELKNYMKEAVKVSSEHPILIDKFLDEATEVDVDAICDG
CEEECCCEEECCEEEEEEECHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHCCCHHEEECC
DDVFIGGIMEHIEEAGIHSGDSACVIPSQTLSPQVIDTIIDHTTNLAKELGVIGLLNIQY
CCEEEHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEE
AVKDGVVYIIEANPRASRTIPYVSKSVGAPLAKIATKVIMGQKLKDMGYIGLAQSKYVSI
EECCCEEEEEECCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEECCCHHHHH
KEAVFPFQKLTGVDPVLSPEMKSTGEAIGIDTDFGRAFYKAQLSANMELPMSGTVFISVK
HHHHCCHHHHCCCCCCCCCCHHCCCCEEECCCHHHHHHHHHHHCCCCCCCCCCEEEEEEC
KKDRKHLEKMAKEFNKFGFNIIATDGTAEEIRSNGVDATIVKKISEGIKGSILEEIQKGN
CHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHCCC
VDLIINTSSGSRAKSDGYYIRRAAVEFNIPYITTIQGALSTIKAIKSVKEGDLGVYSLND
EEEEEECCCCCCCCCCCEEEEEEEEEECCCEEEHHHHHHHHHHHHHHHCCCCCEEEECCC
LENK
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]