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The map label for this gene is bepIM [H]

Identifier: 150400370

GI number: 150400370

Start: 1651035

End: 1652141

Strand: Reverse

Name: bepIM [H]

Synonym: Mevan_1633

Alternate gene names: 150400370

Gene position: 1652141-1651035 (Counterclockwise)

Preceding gene: 150400371

Following gene: 150400365

Centisome position: 96.05

GC content: 33.24

Gene sequence:

>1107_bases
ATGAAAAAATCCCATGAAATACATAAAAATCAAGGCAAATTAATAAGTACAGAAAATATTAAATTAGTTGACACTATGAA
ACCCATTAACTATGCCAAAAAATTTAAAGTTATTTCGTTATTTAGTGGTTGTGGTGGAATGGATTTGGGTTTTAAAGGCG
GTTTTGAAATATTTAAACAACATTATGAACATAACCCCTATGAAATAATATTTTCTAATGATATATCGGATAAAGCATGC
AGAACTTATGAAAGTAATTTTTGCCATTCTTCAGTTTGTGCAGACATAAAGGATATAAAAAATGAAGATATCCCTAATGC
AGACATTGTAATAGGGGGATTCCCTTGCCAAGATTTTAGTCATGCTGGAAAAAGAAAAGGACTTTCTGCTGAAAGAGGTA
GACTTTACTTAGAAATGAAAAGAGTAATTGATTATATTAAACCTATTGCTTTTGTAGCTGAAAATGTAGACGGAATTAGA
ACGAATTCTAAAGGAAAAGATACTACTGCCTTAGACATTATCCTAAAAGATTTTATGGACTCGGGCTACCAAGTAGCCTA
TAAAGTACTTAATACTGCAGATTATGGAGTACCCCAAACTAGAATAAGGGTAATAATAATGGGCATCAGGAATGATTATG
TTGGGGACATAATGTATCCTAAACCCGTTAGGGGTAATGAAGGACTTTTTGAATGGATGACGTCAAAAGAAGCCATTGAC
GATTTATGGGATAAAATAGACTCAAAAGAGGTATTTAATCATTCTTCAAAAGATTATTCTAAAGCTAAATTTTACCCCGG
TAAAAAAATGCAAGGTAATTATAAAATATTGGCAGATCGCCCCTCAATCACAATACGGGCTGAACATCATGGAAACATCG
AAGCCCACTATAGGTCATATAATAATGAAGATCCTGAAGATATGGGGTATTGGAGAAGATTAAGTGTTAGAGAATGTGCA
AGATTGCAGTCGTTTCCGGATGATTTTAATTTTCCATTTTCTGCATCTGAAGCATATAAACAAATTGGTAATGCTGTTCC
ACCGATACTAGGGTGGCATATTGCAAGGGCATTGTACCATACTTTGACGAAAAATAAAAAAAGCTAA

Upstream 100 bases:

>100_bases
AAGAAAGGAAAAAATGATTAAAAATATTCAAAAAATATTATAATTAAAATGTACTATACAGTAATGAGCATATTTAGAAT
AATTTTTGGTGAATAATTTG

Downstream 100 bases:

>100_bases
TTTAATATTTTTTTTCATAAATATCTGATAAATCCGTATTCCTTCCATAATACATTTTTGCTTCGGGTGTTCCATTGAAT
TGACCGTGGCTAAATCTACA

Product: DNA-cytosine methyltransferase

Products: NA

Alternate protein names: M.BepI; Cytosine-specific methyltransferase BepI [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MKKSHEIHKNQGKLISTENIKLVDTMKPINYAKKFKVISLFSGCGGMDLGFKGGFEIFKQHYEHNPYEIIFSNDISDKAC
RTYESNFCHSSVCADIKDIKNEDIPNADIVIGGFPCQDFSHAGKRKGLSAERGRLYLEMKRVIDYIKPIAFVAENVDGIR
TNSKGKDTTALDIILKDFMDSGYQVAYKVLNTADYGVPQTRIRVIIMGIRNDYVGDIMYPKPVRGNEGLFEWMTSKEAID
DLWDKIDSKEVFNHSSKDYSKAKFYPGKKMQGNYKILADRPSITIRAEHHGNIEAHYRSYNNEDPEDMGYWRRLSVRECA
RLQSFPDDFNFPFSASEAYKQIGNAVPPILGWHIARALYHTLTKNKKS

Sequences:

>Translated_368_residues
MKKSHEIHKNQGKLISTENIKLVDTMKPINYAKKFKVISLFSGCGGMDLGFKGGFEIFKQHYEHNPYEIIFSNDISDKAC
RTYESNFCHSSVCADIKDIKNEDIPNADIVIGGFPCQDFSHAGKRKGLSAERGRLYLEMKRVIDYIKPIAFVAENVDGIR
TNSKGKDTTALDIILKDFMDSGYQVAYKVLNTADYGVPQTRIRVIIMGIRNDYVGDIMYPKPVRGNEGLFEWMTSKEAID
DLWDKIDSKEVFNHSSKDYSKAKFYPGKKMQGNYKILADRPSITIRAEHHGNIEAHYRSYNNEDPEDMGYWRRLSVRECA
RLQSFPDDFNFPFSASEAYKQIGNAVPPILGWHIARALYHTLTKNKKS
>Mature_368_residues
MKKSHEIHKNQGKLISTENIKLVDTMKPINYAKKFKVISLFSGCGGMDLGFKGGFEIFKQHYEHNPYEIIFSNDISDKAC
RTYESNFCHSSVCADIKDIKNEDIPNADIVIGGFPCQDFSHAGKRKGLSAERGRLYLEMKRVIDYIKPIAFVAENVDGIR
TNSKGKDTTALDIILKDFMDSGYQVAYKVLNTADYGVPQTRIRVIIMGIRNDYVGDIMYPKPVRGNEGLFEWMTSKEAID
DLWDKIDSKEVFNHSSKDYSKAKFYPGKKMQGNYKILADRPSITIRAEHHGNIEAHYRSYNNEDPEDMGYWRRLSVRECA
RLQSFPDDFNFPFSASEAYKQIGNAVPPILGWHIARALYHTLTKNKKS

Specific function: This methylase recognizes the double-stranded sequence CGCG, causes specific methylation on C-1 on both strands, and protects the DNA from cleavage by the BepI endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788271, Length=383, Percent_Identity=27.154046997389, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 42105; Mature: 42105

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: PS00094 C5_MTASE_1 ; PS00095 C5_MTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSHEIHKNQGKLISTENIKLVDTMKPINYAKKFKVISLFSGCGGMDLGFKGGFEIFKQ
CCCCCCCCCCCCCEEECCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
HYEHNPYEIIFSNDISDKACRTYESNFCHSSVCADIKDIKNEDIPNADIVIGGFPCQDFS
HHCCCCEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHH
HAGKRKGLSAERGRLYLEMKRVIDYIKPIAFVAENVDGIRTNSKGKDTTALDIILKDFMD
HHHHHCCCCCHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHC
SGYQVAYKVLNTADYGVPQTRIRVIIMGIRNDYVGDIMYPKPVRGNEGLFEWMTSKEAID
CCHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
DLWDKIDSKEVFNHSSKDYSKAKFYPGKKMQGNYKILADRPSITIRAEHHGNIEAHYRSY
HHHHHCCHHHHHCCCCCCCCHHHCCCCCCCCCCEEEEECCCCEEEEECCCCCEEEEECCC
NNEDPEDMGYWRRLSVRECARLQSFPDDFNFPFSASEAYKQIGNAVPPILGWHIARALYH
CCCCHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHH
TLTKNKKS
HHHCCCCC
>Mature Secondary Structure
MKKSHEIHKNQGKLISTENIKLVDTMKPINYAKKFKVISLFSGCGGMDLGFKGGFEIFKQ
CCCCCCCCCCCCCEEECCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
HYEHNPYEIIFSNDISDKACRTYESNFCHSSVCADIKDIKNEDIPNADIVIGGFPCQDFS
HHCCCCEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHH
HAGKRKGLSAERGRLYLEMKRVIDYIKPIAFVAENVDGIRTNSKGKDTTALDIILKDFMD
HHHHHCCCCCHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHC
SGYQVAYKVLNTADYGVPQTRIRVIIMGIRNDYVGDIMYPKPVRGNEGLFEWMTSKEAID
CCHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
DLWDKIDSKEVFNHSSKDYSKAKFYPGKKMQGNYKILADRPSITIRAEHHGNIEAHYRSY
HHHHHCCHHHHHCCCCCCCCHHHCCCCCCCCCCEEEEECCCCEEEEECCCCCEEEEECCC
NNEDPEDMGYWRRLSVRECARLQSFPDDFNFPFSASEAYKQIGNAVPPILGWHIARALYH
CCCCHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHH
TLTKNKKS
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2784204 [H]