The gene/protein map for NC_009634 is currently unavailable.
Definition Methanococcus vannielii SB chromosome, complete genome.
Accession NC_009634
Length 1,720,048

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The map label for this gene is proS

Identifier: 150400311

GI number: 150400311

Start: 1587694

End: 1589079

Strand: Reverse

Name: proS

Synonym: Mevan_1574

Alternate gene names: 150400311

Gene position: 1589079-1587694 (Counterclockwise)

Preceding gene: 150400314

Following gene: 150400310

Centisome position: 92.39

GC content: 33.69

Gene sequence:

>1386_bases
ATGGAATTTTCAGAATGGTACAGCGAAATTTTAGAAAAGGCTGGAATTTACGATTTGAGGTACCCTATAAAAGGATGCGG
TGTTTATTTACCTTATGGTTTCAAAATCAGACGTTATTCATTTGAAATTCTAAGAAAGTTGTTAGATGACACGAACCATG
ATGAAACACTATTTCCAATGCTAATTCCTGAAAACTTGCTTGCAAAAGAAGGAGAACATATTAAAGGGTTTGAAGATGAA
GTATACTGGGTAACCCATGGTGGAAAAAGCCCTTTAGAAATAAAACTCGCTTTAAGGCCAACTTCTGAAACTACGATGTA
CTACATGATGAAACAGTGGATTAAAGTTCATACTGATCTTCCAATGAAACTATATCAAGTTGTGAACACTTTTAGATATG
AAACAAAACATACTCGACCATTAATTAGATTAAGAGAAATAATGAGTTTTAAAGAGGCCCATACAGCCCATTCAACCCAG
GACGACTGTAACAAGCAGATAAAAGAAGCTATTGAAATATATGGCAAATTTTTTGATGAAATATGTATCCCAGTCATTAT
TTCAAAAAGACCCGAATGGGATAAGTTTCCCGGTGCAGACTACACAATGGCGTTTGATACAATTTTCCCAGATGGAAAAA
CGATGCAGATTGGAACGGTTCATAATTTGGGCCAAAATTTTGCAAAGACGTTTGAACTCGAATTTGAAACTCCTGACGGA
AATAAAGACTATGCCTACCAAACCTGCTATGGAATATCTGATAGGGCGATTGCATCATTAATTGCAATTCATGGGGATGA
AAAAGGACTTGTAATTCCAATAGATGTTGCACCAATTCAGATTGTACTTATTCCATTACTATTTAAAGGAAAAGAAGAAA
TTGTAATTGAAAAAATTAAAGAATTAAGTAAAATTTTAAAAACAAAATACCGAGTTCATGTTGATGATAGAGACATTCGC
CCAGGCCGAAAATATAACGACTGGGAGATAAAAGGAGTTCCTTTAAGGATTGAAATTGGCCCACGAGATATTGAAGAAGG
AAAGGCAGTTATTGTTAGAAGAGATAATGGCCAAAAAATGACGGTTGAAGCTTTTAACATTTTAAACGAAGTAGAAAAAA
CCTTTGAAGATTATAAGGCTAGTTTATTACAAAAAGCGACTGAAAAACTTGAAAAATACATAACCGTAATAGAAAACGAT
AAAAAAGACCTTTTATTACTTTCAGAAAAGGTAAAATTTGCTCTTTCTGAAAGTAAAGGCATTATACTCGTCCCATACAT
TGAATCAATCTATAATGAGGAATTTGAAGGGTTAATTGATGCATCCGTACTTGGATTAACTACTTACAATGGTAAAGAGT
ACATCTCAATTGCAAGAACTTACTAA

Upstream 100 bases:

>100_bases
ATTATAATCAATAATCTACAAAACTACAAATATCCTTTGAATCGGCATGTGCGTTCATTATGTATTAATTATGTACTTAT
ATACTATTTATGGTGAAAAA

Downstream 100 bases:

>100_bases
ATGTTTTTAAATTAAATTCATTGTTTTCTAAATACTACTATTTCTTTTATTTTTAAAACCGTAGACTATATAAAGAATAT
ATTATAAATTGATTTATATT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MEFSEWYSEILEKAGIYDLRYPIKGCGVYLPYGFKIRRYSFEILRKLLDDTNHDETLFPMLIPENLLAKEGEHIKGFEDE
VYWVTHGGKSPLEIKLALRPTSETTMYYMMKQWIKVHTDLPMKLYQVVNTFRYETKHTRPLIRLREIMSFKEAHTAHSTQ
DDCNKQIKEAIEIYGKFFDEICIPVIISKRPEWDKFPGADYTMAFDTIFPDGKTMQIGTVHNLGQNFAKTFELEFETPDG
NKDYAYQTCYGISDRAIASLIAIHGDEKGLVIPIDVAPIQIVLIPLLFKGKEEIVIEKIKELSKILKTKYRVHVDDRDIR
PGRKYNDWEIKGVPLRIEIGPRDIEEGKAVIVRRDNGQKMTVEAFNILNEVEKTFEDYKASLLQKATEKLEKYITVIEND
KKDLLLLSEKVKFALSESKGIILVPYIESIYNEEFEGLIDASVLGLTTYNGKEYISIARTY

Sequences:

>Translated_461_residues
MEFSEWYSEILEKAGIYDLRYPIKGCGVYLPYGFKIRRYSFEILRKLLDDTNHDETLFPMLIPENLLAKEGEHIKGFEDE
VYWVTHGGKSPLEIKLALRPTSETTMYYMMKQWIKVHTDLPMKLYQVVNTFRYETKHTRPLIRLREIMSFKEAHTAHSTQ
DDCNKQIKEAIEIYGKFFDEICIPVIISKRPEWDKFPGADYTMAFDTIFPDGKTMQIGTVHNLGQNFAKTFELEFETPDG
NKDYAYQTCYGISDRAIASLIAIHGDEKGLVIPIDVAPIQIVLIPLLFKGKEEIVIEKIKELSKILKTKYRVHVDDRDIR
PGRKYNDWEIKGVPLRIEIGPRDIEEGKAVIVRRDNGQKMTVEAFNILNEVEKTFEDYKASLLQKATEKLEKYITVIEND
KKDLLLLSEKVKFALSESKGIILVPYIESIYNEEFEGLIDASVLGLTTYNGKEYISIARTY
>Mature_461_residues
MEFSEWYSEILEKAGIYDLRYPIKGCGVYLPYGFKIRRYSFEILRKLLDDTNHDETLFPMLIPENLLAKEGEHIKGFEDE
VYWVTHGGKSPLEIKLALRPTSETTMYYMMKQWIKVHTDLPMKLYQVVNTFRYETKHTRPLIRLREIMSFKEAHTAHSTQ
DDCNKQIKEAIEIYGKFFDEICIPVIISKRPEWDKFPGADYTMAFDTIFPDGKTMQIGTVHNLGQNFAKTFELEFETPDG
NKDYAYQTCYGISDRAIASLIAIHGDEKGLVIPIDVAPIQIVLIPLLFKGKEEIVIEKIKELSKILKTKYRVHVDDRDIR
PGRKYNDWEIKGVPLRIEIGPRDIEEGKAVIVRRDNGQKMTVEAFNILNEVEKTFEDYKASLLQKATEKLEKYITVIEND
KKDLLLLSEKVKFALSESKGIILVPYIESIYNEEFEGLIDASVLGLTTYNGKEYISIARTY

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily

Homologues:

Organism=Homo sapiens, GI62241042, Length=444, Percent_Identity=34.6846846846847, Blast_Score=287, Evalue=1e-77,
Organism=Escherichia coli, GI1786392, Length=172, Percent_Identity=27.3255813953488, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71991342, Length=435, Percent_Identity=33.7931034482759, Blast_Score=266, Evalue=2e-71,
Organism=Caenorhabditis elegans, GI71991346, Length=355, Percent_Identity=35.7746478873239, Blast_Score=253, Evalue=2e-67,
Organism=Saccharomyces cerevisiae, GI6321808, Length=450, Percent_Identity=35.1111111111111, Blast_Score=264, Evalue=2e-71,
Organism=Drosophila melanogaster, GI24649468, Length=447, Percent_Identity=33.3333333333333, Blast_Score=250, Evalue=1e-66,
Organism=Drosophila melanogaster, GI24649466, Length=447, Percent_Identity=33.3333333333333, Blast_Score=250, Evalue=1e-66,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_METVS (A6USJ4)

Other databases:

- EMBL:   CP000742
- RefSeq:   YP_001324078.1
- ProteinModelPortal:   A6USJ4
- SMR:   A6USJ4
- STRING:   A6USJ4
- GeneID:   5325457
- GenomeReviews:   CP000742_GR
- KEGG:   mvn:Mevan_1574
- eggNOG:   arNOG04466
- HOGENOM:   HBG334108
- OMA:   PGWKFNE
- ProtClustDB:   PRK08661
- GO:   GO:0005737
- HAMAP:   MF_01571
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004499
- InterPro:   IPR017449
- InterPro:   IPR015264
- InterPro:   IPR016061
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.30.110.30
- PANTHER:   PTHR11451:SF6
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00408

Pfam domain/function: PF03129 HGTP_anticodon; PF09181 ProRS-C_2; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd; SSF64586 Pro-tRNA_synth_II_C

EC number: =6.1.1.15

Molecular weight: Translated: 53496; Mature: 53496

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MEFSEWYSEILEKAGIYDLRYPIKGCGVYLPYGFKIRRYSFEILRKLLDDTNHDETLFPM
CCHHHHHHHHHHHCCCEEEECCCCCCCEEECCCEEEEHHHHHHHHHHHCCCCCCCEEEEE
LIPENLLAKEGEHIKGFEDEVYWVTHGGKSPLEIKLALRPTSETTMYYMMKQWIKVHTDL
ECCCHHHHCCCCCCCCCCCCEEEEEECCCCCEEEEEEEECCCCHHHHHHHHHHHHHHCCC
PMKLYQVVNTFRYETKHTRPLIRLREIMSFKEAHTAHSTQDDCNKQIKEAIEIYGKFFDE
CHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
ICIPVIISKRPEWDKFPGADYTMAFDTIFPDGKTMQIGTVHNLGQNFAKTFELEFETPDG
HHHHHEECCCCCCCCCCCCCEEEEEEEECCCCCEEEECCHHHHHHHHHEEEEEEEECCCC
NKDYAYQTCYGISDRAIASLIAIHGDEKGLVIPIDVAPIQIVLIPLLFKGKEEIVIEKIK
CCCCCEEEECCCCHHHHHHHHHEECCCCCEEEEECCCCHHHHEEHHHHCCCHHHHHHHHH
ELSKILKTKYRVHVDDRDIRPGRKYNDWEIKGVPLRIEIGPRDIEEGKAVIVRRDNGQKM
HHHHHHHHHEEEEECCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCCEEEEECCCCCEE
TVEAFNILNEVEKTFEDYKASLLQKATEKLEKYITVIENDKKDLLLLSEKVKFALSESKG
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHCCCCCC
IILVPYIESIYNEEFEGLIDASVLGLTTYNGKEYISIARTY
EEEEECHHHHCCHHHHHHHCCCEEEEEEECCCEEEEEEECC
>Mature Secondary Structure
MEFSEWYSEILEKAGIYDLRYPIKGCGVYLPYGFKIRRYSFEILRKLLDDTNHDETLFPM
CCHHHHHHHHHHHCCCEEEECCCCCCCEEECCCEEEEHHHHHHHHHHHCCCCCCCEEEEE
LIPENLLAKEGEHIKGFEDEVYWVTHGGKSPLEIKLALRPTSETTMYYMMKQWIKVHTDL
ECCCHHHHCCCCCCCCCCCCEEEEEECCCCCEEEEEEEECCCCHHHHHHHHHHHHHHCCC
PMKLYQVVNTFRYETKHTRPLIRLREIMSFKEAHTAHSTQDDCNKQIKEAIEIYGKFFDE
CHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
ICIPVIISKRPEWDKFPGADYTMAFDTIFPDGKTMQIGTVHNLGQNFAKTFELEFETPDG
HHHHHEECCCCCCCCCCCCCEEEEEEEECCCCCEEEECCHHHHHHHHHEEEEEEEECCCC
NKDYAYQTCYGISDRAIASLIAIHGDEKGLVIPIDVAPIQIVLIPLLFKGKEEIVIEKIK
CCCCCEEEECCCCHHHHHHHHHEECCCCCEEEEECCCCHHHHEEHHHHCCCHHHHHHHHH
ELSKILKTKYRVHVDDRDIRPGRKYNDWEIKGVPLRIEIGPRDIEEGKAVIVRRDNGQKM
HHHHHHHHHEEEEECCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCCEEEEECCCCCEE
TVEAFNILNEVEKTFEDYKASLLQKATEKLEKYITVIENDKKDLLLLSEKVKFALSESKG
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHCCCCCC
IILVPYIESIYNEEFEGLIDASVLGLTTYNGKEYISIARTY
EEEEECHHHHCCHHHHHHHCCCEEEEEEECCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA