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The map label for this gene is rluB [H]

Identifier: 150010293

GI number: 150010293

Start: 4544218

End: 4545705

Strand: Direct

Name: rluB [H]

Synonym: BDI_3726

Alternate gene names: 150010293

Gene position: 4544218-4545705 (Clockwise)

Preceding gene: 150010292

Following gene: 150010294

Centisome position: 94.45

GC content: 47.04

Gene sequence:

>1488_bases
ATGAGTACAGAAGAAAGAGATGAATCTCAACCGCGTCCAAAAAAAGTGATACCAAGTATCAGACGGGAAAACACAGACCA
AGAAGGTGAAAGAAGACCTTACAATCAGGGTTATAATAAACCTGAGGGTAGCTATGAACGTAGACCGTATCGGTCTTATG
GAGAAAATAACCGTCCTTCATACGGGGATCGTCCGAGCCGTCCGCGTTCTTACGATAATAACCGTGAAGGCGGGTATGGC
AACAACCGTGAGGGGGGCTATGGTAATAGTCGTCCATCTTATGGCAACAATCGCGATGGTGGGTATGGAAATAATCGCCC
GTCTTATGGCAACAACCGTGAAGGTGGCTACAACAGCGGCCGCTCGTCTTATGGAAATAACCGCCCGTCATATGGGAATG
GAGGAGGGCAATACAATCGTCCGTCTCGTCCTAATTATGATGGACCGGCAAAGGCTTATTCCGCTAGTCCTTCCGGAGAG
GGAATGTCTGCGGATGGTATGAAGAAAAGAAGACCTAGAGTAGGTGATACCCGGGTTGATTACAATGATTCACGTTCAAG
CGGCGGGGACGGTTACGCACCCAGACGTTCGTATGGTAACGATAACTCTTATGGCGGTAATCGTCAAGGGGGTGGTGGAT
ATGGAAATAATCGCCGTCCTTCTTACAATAATAATCGCCGTTCCGGTGGAGGAGGTGGTTTCCAAAGACAGCAGGGTTAC
AACATGCGTCCGAAACAGATCAAGTATAAGGAAGTCTTGGCGGATCCGAACGAACCGATTCGTTTGAACAAATTCTTATC
TAACGCTGGTGTTTGCTCTCGTCGTGAGGCGGATGAGTTTATCCAGAAAGGGGCTGTTAAGGTTAATGATGTCGTGGTAA
CGGAATTGGGTACTAAGATTACTCGTCAAGATGTTGTTACATTTAACGAGAAGCCGGTTCAGATCGAGAGCAAGGTGTAT
ATCGTATTGAACAAGCCGAAGAATTGCGTAACGACTTCTGATGATCCTCAAGAACGCTTGACAGTAATGGATTTGGTGAA
GAACGCTTGCCAAGAACGTATTTATCCGGTAGGCCGTTTGGACCGCAATACGACAGGTGTCTTGTTATTGACGAACGATG
GTGATTTAGCTTCTAAATTGACTCACCCATCTTTCAAGAAGAAGAAGATCTACCATGTGTGGTTGGATAAGAACGTGGCT
ATCGAGGATATGGAGAAAATCGCCAACGGCCTTGAGCTTGAGGATGGGGAGATCCATGCGGACGCTATCAGCTATGCTAG
CGAGGACGATAAGAGCCAAGTGGGTATCGAGATCCATTCCGGCCGTAACCGTATCGTGCGCCGTATTTTCGAATCGTTGG
GATATCATGTGACGAAATTGGATCGTGTTTATTTCGCCGGTTTGACTAAGAAGAACTTAGGCCGCGGTAAATGGCGTTAC
TTAAATGAAAGGGAAGTGAACGCTCTTAGGATGGGAGCTTTCGAATAA

Upstream 100 bases:

>100_bases
AATTAAGGGCTATAACGCCTCATAATTATACAGGAATTTAGTGATAATACAGGTAGCCGTGAGGCTACCAAGTTTTTAAC
CATTGTTTAATTTATAACAA

Downstream 100 bases:

>100_bases
AAAAAACGATACAGATGGAAACAATTAAACGAACAAAAATTGTAGACGTACTGAAAAGCGACGCTTTCGGTACTACCGTG
AACGTGAAAGGATGGGTTCG

Product: ribosomal large subunit pseudouridine synthase B

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MSTEERDESQPRPKKVIPSIRRENTDQEGERRPYNQGYNKPEGSYERRPYRSYGENNRPSYGDRPSRPRSYDNNREGGYG
NNREGGYGNSRPSYGNNRDGGYGNNRPSYGNNREGGYNSGRSSYGNNRPSYGNGGGQYNRPSRPNYDGPAKAYSASPSGE
GMSADGMKKRRPRVGDTRVDYNDSRSSGGDGYAPRRSYGNDNSYGGNRQGGGGYGNNRRPSYNNNRRSGGGGGFQRQQGY
NMRPKQIKYKEVLADPNEPIRLNKFLSNAGVCSRREADEFIQKGAVKVNDVVVTELGTKITRQDVVTFNEKPVQIESKVY
IVLNKPKNCVTTSDDPQERLTVMDLVKNACQERIYPVGRLDRNTTGVLLLTNDGDLASKLTHPSFKKKKIYHVWLDKNVA
IEDMEKIANGLELEDGEIHADAISYASEDDKSQVGIEIHSGRNRIVRRIFESLGYHVTKLDRVYFAGLTKKNLGRGKWRY
LNEREVNALRMGAFE

Sequences:

>Translated_495_residues
MSTEERDESQPRPKKVIPSIRRENTDQEGERRPYNQGYNKPEGSYERRPYRSYGENNRPSYGDRPSRPRSYDNNREGGYG
NNREGGYGNSRPSYGNNRDGGYGNNRPSYGNNREGGYNSGRSSYGNNRPSYGNGGGQYNRPSRPNYDGPAKAYSASPSGE
GMSADGMKKRRPRVGDTRVDYNDSRSSGGDGYAPRRSYGNDNSYGGNRQGGGGYGNNRRPSYNNNRRSGGGGGFQRQQGY
NMRPKQIKYKEVLADPNEPIRLNKFLSNAGVCSRREADEFIQKGAVKVNDVVVTELGTKITRQDVVTFNEKPVQIESKVY
IVLNKPKNCVTTSDDPQERLTVMDLVKNACQERIYPVGRLDRNTTGVLLLTNDGDLASKLTHPSFKKKKIYHVWLDKNVA
IEDMEKIANGLELEDGEIHADAISYASEDDKSQVGIEIHSGRNRIVRRIFESLGYHVTKLDRVYFAGLTKKNLGRGKWRY
LNEREVNALRMGAFE
>Mature_494_residues
STEERDESQPRPKKVIPSIRRENTDQEGERRPYNQGYNKPEGSYERRPYRSYGENNRPSYGDRPSRPRSYDNNREGGYGN
NREGGYGNSRPSYGNNRDGGYGNNRPSYGNNREGGYNSGRSSYGNNRPSYGNGGGQYNRPSRPNYDGPAKAYSASPSGEG
MSADGMKKRRPRVGDTRVDYNDSRSSGGDGYAPRRSYGNDNSYGGNRQGGGGYGNNRRPSYNNNRRSGGGGGFQRQQGYN
MRPKQIKYKEVLADPNEPIRLNKFLSNAGVCSRREADEFIQKGAVKVNDVVVTELGTKITRQDVVTFNEKPVQIESKVYI
VLNKPKNCVTTSDDPQERLTVMDLVKNACQERIYPVGRLDRNTTGVLLLTNDGDLASKLTHPSFKKKKIYHVWLDKNVAI
EDMEKIANGLELEDGEIHADAISYASEDDKSQVGIEIHSGRNRIVRRIFESLGYHVTKLDRVYFAGLTKKNLGRGKWRYL
NEREVNALRMGAFE

Specific function: Responsible for synthesis of pseudouridine from uracil- 2633 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=237, Percent_Identity=33.3333333333333, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1787524, Length=241, Percent_Identity=34.0248962655602, Blast_Score=112, Evalue=8e-26,
Organism=Escherichia coli, GI1788510, Length=232, Percent_Identity=30.6034482758621, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI87081838, Length=179, Percent_Identity=28.4916201117318, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 55726; Mature: 55595

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCHHHCCCCC
>Mature Secondary Structure 
STEERDESQPRPKKVIPSIRRENTDQEGERRPYNQGYNKPEGSYERRPYRSYGENNRPS
CCCCCCCCCCCCHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCCCCCCCHHCCCCCCCC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CCCCCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7934829; 9384377; 9888802 [H]