Click here to switch to the map view.

The map label for this gene is purB [H]

Identifier: 150010292

GI number: 150010292

Start: 4542809

End: 4544158

Strand: Direct

Name: purB [H]

Synonym: BDI_3725

Alternate gene names: 150010292

Gene position: 4542809-4544158 (Clockwise)

Preceding gene: 150010291

Following gene: 150010293

Centisome position: 94.42

GC content: 44.3

Gene sequence:

>1350_bases
ATGTATATGGTATTATCGACACTGACTGCTATTTCGCCCGTGGATGGGCGATACAGGAATAAGGCTGAGAATCTGGCGGC
TTATTTTTCAGAATACGCACTTATCAAGTATAGGGTGCGAGTTGAGATCGAGTATTTTATTACTCTATCTGAGTTTCTTC
CGCAATTAAGCGAGTTAAATGCGGCGGAAGTAAAAAAGGGGCTCCGGAAAATTTATCAGGAGTTTACGGAGGAGGACGCT
TTACGAGTGAAGGAGATCGAGAGTGTGACAAACCACGATGTGAAAGCGGTGGAGTACTTTATTAAGGAAAAAACAAATCA
TTTCCTTGCGGAGAAATACCACGAGTTTATTCATTTTGGATTGACTTCTCAGGATATTAATAACACATCTGTGCCTTTAT
CGATCAAGGACGCTTTGCAAGAGGTGTATTATCCGGGTTTGGAAGAGGTGATCGGCGCCTTGAAAAAATACGCCGAGGAA
TGGATGGATATTCCGATGTTGGCTAAGACACATGGGCAACCGGCATCTCCGACTCGTTTGGGTAAGGAGGTGATGGTCTT
TGTTTACCGTTTGGAGCAACAGTTGGCATTGTTAAAGGCCGTTCCTATCTCCGCTAAATTCGGTGGGGCTACAGGAAACT
TCAATGCGCATCATGTAGCGTATCCGGAATATGATTGGAGGGCATTCGGTAACAAGTTCGTTAGTGAGGTGTTAGGCTTG
AAGCGTGAGGAGTGGACTACCCAGATCTCTAATTATGATAATCTGGCAGCGATCTTCGATGGCTTGAAGCGTATCAATAC
GATCTTGATCGACTTGAATCGTGACTTCTGGCAATATATCTCCATGGAGTATTTCAAGCAAAAGATCAAGGCGGGTGAGA
TCGGTTCGTCTGCTATGCCACATAAGGTGAACCCGATTGATTTCGAGAACGCTGAGGGTAATCTAGGGATCGCTAACGCT
ATTTTAGAGCATTTGGCTACGAAATTGCCGGTATCTCGTTTACAAAGAGACTTGACGGATTCTACCGTGTTGAGAAACGT
AGGCGTGCCTTTGGCACATATAGAAATTGCCTTCAAGAGCTTAACAAAAGGCTTGGGTAAATTGTTATTGAACGAGAACG
CCCTAAGTAGGGATTTGAATAATTGCTGGGCTGTGGTAGCCGAGGGTATCCAGACGGTCTTACGCCGTGAGGGTTATCCG
AAGCCTTACGAGGCATTAAAAGCTTTGACACGTACGAACCAGACTGTTACGGAGCAATCTATCAAGGAGTTTATCGAAAC
CTTGAATGTTAGCGACCGGATTAAAGATGAATTAAGGGCTATAACGCCTCATAATTATACAGGAATTTAG

Upstream 100 bases:

>100_bases
GTGTCTGATGGTCCTAAAAACGGGGCATTTAGTTAAAAATAACGATTGCTTACCTTATTATTCAATGGAAAAGACTTAAC
TTTGCGACTCTATATTAATA

Downstream 100 bases:

>100_bases
TGATAATACAGGTAGCCGTGAGGCTACCAAGTTTTTAACCATTGTTTAATTTATAACAAATGAGTACAGAAGAAAGAGAT
GAATCTCAACCGCGTCCAAA

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MYMVLSTLTAISPVDGRYRNKAENLAAYFSEYALIKYRVRVEIEYFITLSEFLPQLSELNAAEVKKGLRKIYQEFTEEDA
LRVKEIESVTNHDVKAVEYFIKEKTNHFLAEKYHEFIHFGLTSQDINNTSVPLSIKDALQEVYYPGLEEVIGALKKYAEE
WMDIPMLAKTHGQPASPTRLGKEVMVFVYRLEQQLALLKAVPISAKFGGATGNFNAHHVAYPEYDWRAFGNKFVSEVLGL
KREEWTTQISNYDNLAAIFDGLKRINTILIDLNRDFWQYISMEYFKQKIKAGEIGSSAMPHKVNPIDFENAEGNLGIANA
ILEHLATKLPVSRLQRDLTDSTVLRNVGVPLAHIEIAFKSLTKGLGKLLLNENALSRDLNNCWAVVAEGIQTVLRREGYP
KPYEALKALTRTNQTVTEQSIKEFIETLNVSDRIKDELRAITPHNYTGI

Sequences:

>Translated_449_residues
MYMVLSTLTAISPVDGRYRNKAENLAAYFSEYALIKYRVRVEIEYFITLSEFLPQLSELNAAEVKKGLRKIYQEFTEEDA
LRVKEIESVTNHDVKAVEYFIKEKTNHFLAEKYHEFIHFGLTSQDINNTSVPLSIKDALQEVYYPGLEEVIGALKKYAEE
WMDIPMLAKTHGQPASPTRLGKEVMVFVYRLEQQLALLKAVPISAKFGGATGNFNAHHVAYPEYDWRAFGNKFVSEVLGL
KREEWTTQISNYDNLAAIFDGLKRINTILIDLNRDFWQYISMEYFKQKIKAGEIGSSAMPHKVNPIDFENAEGNLGIANA
ILEHLATKLPVSRLQRDLTDSTVLRNVGVPLAHIEIAFKSLTKGLGKLLLNENALSRDLNNCWAVVAEGIQTVLRREGYP
KPYEALKALTRTNQTVTEQSIKEFIETLNVSDRIKDELRAITPHNYTGI
>Mature_449_residues
MYMVLSTLTAISPVDGRYRNKAENLAAYFSEYALIKYRVRVEIEYFITLSEFLPQLSELNAAEVKKGLRKIYQEFTEEDA
LRVKEIESVTNHDVKAVEYFIKEKTNHFLAEKYHEFIHFGLTSQDINNTSVPLSIKDALQEVYYPGLEEVIGALKKYAEE
WMDIPMLAKTHGQPASPTRLGKEVMVFVYRLEQQLALLKAVPISAKFGGATGNFNAHHVAYPEYDWRAFGNKFVSEVLGL
KREEWTTQISNYDNLAAIFDGLKRINTILIDLNRDFWQYISMEYFKQKIKAGEIGSSAMPHKVNPIDFENAEGNLGIANA
ILEHLATKLPVSRLQRDLTDSTVLRNVGVPLAHIEIAFKSLTKGLGKLLLNENALSRDLNNCWAVVAEGIQTVLRREGYP
KPYEALKALTRTNQTVTEQSIKEFIETLNVSDRIKDELRAITPHNYTGI

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI183227688, Length=435, Percent_Identity=25.2873563218391, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI1787376, Length=449, Percent_Identity=51.6703786191537, Blast_Score=444, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6323391, Length=308, Percent_Identity=24.025974025974, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24647570, Length=253, Percent_Identity=26.4822134387352, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013539
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 51134; Mature: 51134

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYMVLSTLTAISPVDGRYRNKAENLAAYFSEYALIKYRVRVEIEYFITLSEFLPQLSELN
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHEEEEEEEEEEHEEEHHHHHHHHHCCC
AAEVKKGLRKIYQEFTEEDALRVKEIESVTNHDVKAVEYFIKEKTNHFLAEKYHEFIHFG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LTSQDINNTSVPLSIKDALQEVYYPGLEEVIGALKKYAEEWMDIPMLAKTHGQPASPTRL
CCCCCCCCCCCCEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHH
GKEVMVFVYRLEQQLALLKAVPISAKFGGATGNFNAHHVAYPEYDWRAFGNKFVSEVLGL
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHCC
KREEWTTQISNYDNLAAIFDGLKRINTILIDLNRDFWQYISMEYFKQKIKAGEIGSSAMP
CHHHHHHHHCCCCHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCC
HKVNPIDFENAEGNLGIANAILEHLATKLPVSRLQRDLTDSTVLRNVGVPLAHIEIAFKS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LTKGLGKLLLNENALSRDLNNCWAVVAEGIQTVLRREGYPKPYEALKALTRTNQTVTEQS
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHH
IKEFIETLNVSDRIKDELRAITPHNYTGI
HHHHHHHCCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MYMVLSTLTAISPVDGRYRNKAENLAAYFSEYALIKYRVRVEIEYFITLSEFLPQLSELN
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHEEEEEEEEEEHEEEHHHHHHHHHCCC
AAEVKKGLRKIYQEFTEEDALRVKEIESVTNHDVKAVEYFIKEKTNHFLAEKYHEFIHFG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LTSQDINNTSVPLSIKDALQEVYYPGLEEVIGALKKYAEEWMDIPMLAKTHGQPASPTRL
CCCCCCCCCCCCEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHH
GKEVMVFVYRLEQQLALLKAVPISAKFGGATGNFNAHHVAYPEYDWRAFGNKFVSEVLGL
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHCC
KREEWTTQISNYDNLAAIFDGLKRINTILIDLNRDFWQYISMEYFKQKIKAGEIGSSAMP
CHHHHHHHHCCCCHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCC
HKVNPIDFENAEGNLGIANAILEHLATKLPVSRLQRDLTDSTVLRNVGVPLAHIEIAFKS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LTKGLGKLLLNENALSRDLNNCWAVVAEGIQTVLRREGYPKPYEALKALTRTNQTVTEQS
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHH
IKEFIETLNVSDRIKDELRAITPHNYTGI
HHHHHHHCCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]