Click here to switch to the map view.

The map label for this gene is rffG [H]

Identifier: 150010203

GI number: 150010203

Start: 4439000

End: 4439950

Strand: Direct

Name: rffG [H]

Synonym: BDI_3626

Alternate gene names: 150010203

Gene position: 4439000-4439950 (Clockwise)

Preceding gene: 150010202

Following gene: 150010204

Centisome position: 92.26

GC content: 39.12

Gene sequence:

>951_bases
TTGATAACTGGAGCATCTGGTTTGATATGTTCAGCTTTTATAGATCAATTAATGGGCGATAATGAACGAGAAAATTTAGG
TATAAAGATCTATGCAATAAGTAGGAATAAGGATTACGCTCAAAAAAGGTTTCAACAGTATTGGAATAATCCGTTATTTA
CATTTATGCAACATGACATTATAAAGCCTCTTTCATTGGATGTTCCGCTAGATTATATTATCCATGGAGCGAGTAACGCC
TCTCCGAAACGTTACGCTACAGATCCGGTCGGAACTATGAAAGCCAATCTTTGGGGCGTGGCGAATACGTTGGATTTGGC
CAAGGAGAAACAAGCTCGCCTTTTGTATATTTCATCTGGTGAAGTCTATGGAGAAGGAGATGGAACGGATTTTACGGAAA
CTTATAGTGGCTATGTGGATTGTTTAAATCTCAGAGCTTGTTACCCTTCTGGTAAGCGAGCTTCTGAGACGTTGTGTATT
GCTTATAAAGAGCAATACGGTGTAGATATTGTAATTGCTCGTCCATGTCATATTTATGGTTCAGATATTGGACGTGACGA
TCGAGCATTCGCTCAGTTTTTGCGAAAGGCCCAAGTGGGTGAAGATATTATATTGAAGAGCGAGGGACAGCAGATTCGTT
CTTATTGCCATGTAATGGATTGTGCTTCGGCGTTGTTCTATATCTTATTACGAGGAAAGGATGGAGAGGCTTATAATATT
GCCAATCGAGAATCGATATTATCCATTAAAGAGTTGGCGGAAATGATAGCGAATATGGCTGGGGTAAAGATAGTATATGA
TATTCCTTCCGAGCAAGAAGTAAAGGGGTATTCTAAAGTGCAACGAGCGGTCTTGGATTCTTCCAAATTAGAGTCTTTGG
GTTGGAAGCCGTTTATCTTTTTAGAGGATGGATTGAATAGAGTTTTAAATAGTATGATGTGTCATAAATGA

Upstream 100 bases:

>100_bases
AAGATATAGAGATTTTTACTTCGTTGTTAGATTATAGGAAAGAGGAGGGATTGAAGTGAATGTTGGAACGTTGATAGGTG
ATTTAAAAAGCAAGTCTATT

Downstream 100 bases:

>100_bases
AACGAGTCGGAGATTATATAAATGCTAATTTGTCACAAATAAGTGTTCTTCGGGAACTTACTGATAAAGAAAGGGAACGA
TTGAGGAAGTGCTTGTTCTC

Product: putative nucleotide-sugar dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MITGASGLICSAFIDQLMGDNERENLGIKIYAISRNKDYAQKRFQQYWNNPLFTFMQHDIIKPLSLDVPLDYIIHGASNA
SPKRYATDPVGTMKANLWGVANTLDLAKEKQARLLYISSGEVYGEGDGTDFTETYSGYVDCLNLRACYPSGKRASETLCI
AYKEQYGVDIVIARPCHIYGSDIGRDDRAFAQFLRKAQVGEDIILKSEGQQIRSYCHVMDCASALFYILLRGKDGEAYNI
ANRESILSIKELAEMIANMAGVKIVYDIPSEQEVKGYSKVQRAVLDSSKLESLGWKPFIFLEDGLNRVLNSMMCHK

Sequences:

>Translated_316_residues
MITGASGLICSAFIDQLMGDNERENLGIKIYAISRNKDYAQKRFQQYWNNPLFTFMQHDIIKPLSLDVPLDYIIHGASNA
SPKRYATDPVGTMKANLWGVANTLDLAKEKQARLLYISSGEVYGEGDGTDFTETYSGYVDCLNLRACYPSGKRASETLCI
AYKEQYGVDIVIARPCHIYGSDIGRDDRAFAQFLRKAQVGEDIILKSEGQQIRSYCHVMDCASALFYILLRGKDGEAYNI
ANRESILSIKELAEMIANMAGVKIVYDIPSEQEVKGYSKVQRAVLDSSKLESLGWKPFIFLEDGLNRVLNSMMCHK
>Mature_316_residues
MITGASGLICSAFIDQLMGDNERENLGIKIYAISRNKDYAQKRFQQYWNNPLFTFMQHDIIKPLSLDVPLDYIIHGASNA
SPKRYATDPVGTMKANLWGVANTLDLAKEKQARLLYISSGEVYGEGDGTDFTETYSGYVDCLNLRACYPSGKRASETLCI
AYKEQYGVDIVIARPCHIYGSDIGRDDRAFAQFLRKAQVGEDIILKSEGQQIRSYCHVMDCASALFYILLRGKDGEAYNI
ANRESILSIKELAEMIANMAGVKIVYDIPSEQEVKGYSKVQRAVLDSSKLESLGWKPFIFLEDGLNRVLNSMMCHK

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=318, Percent_Identity=33.3333333333333, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI7657641, Length=326, Percent_Identity=24.5398773006135, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1788353, Length=346, Percent_Identity=23.121387283237, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1788589, Length=351, Percent_Identity=24.2165242165242, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI48994969, Length=349, Percent_Identity=23.7822349570201, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17539532, Length=308, Percent_Identity=31.8181818181818, Blast_Score=147, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI115532424, Length=326, Percent_Identity=24.5398773006135, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17568069, Length=329, Percent_Identity=24.9240121580547, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21356223, Length=313, Percent_Identity=33.2268370607029, Blast_Score=147, Evalue=8e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 35459; Mature: 35459

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITGASGLICSAFIDQLMGDNERENLGIKIYAISRNKDYAQKRFQQYWNNPLFTFMQHDI
CCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCHHHHHHHHHC
IKPLSLDVPLDYIIHGASNASPKRYATDPVGTMKANLWGVANTLDLAKEKQARLLYISSG
CCCCCCCCCHHHEEECCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHCCCCEEEEEECC
EVYGEGDGTDFTETYSGYVDCLNLRACYPSGKRASETLCIAYKEQYGVDIVIARPCHIYG
EEEECCCCCCHHHHHHHHHHHHEEEEECCCCCCCCCEEEEEEECCCCEEEEEECCCEEEC
SDIGRDDRAFAQFLRKAQVGEDIILKSEGQQIRSYCHVMDCASALFYILLRGKDGEAYNI
CCCCCCHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHEEEECCCCCEEEC
ANRESILSIKELAEMIANMAGVKIVYDIPSEQEVKGYSKVQRAVLDSSKLESLGWKPFIF
CCHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
LEDGLNRVLNSMMCHK
EHHHHHHHHHHHHCCC
>Mature Secondary Structure
MITGASGLICSAFIDQLMGDNERENLGIKIYAISRNKDYAQKRFQQYWNNPLFTFMQHDI
CCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCHHHHHHHHHC
IKPLSLDVPLDYIIHGASNASPKRYATDPVGTMKANLWGVANTLDLAKEKQARLLYISSG
CCCCCCCCCHHHEEECCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHCCCCEEEEEECC
EVYGEGDGTDFTETYSGYVDCLNLRACYPSGKRASETLCIAYKEQYGVDIVIARPCHIYG
EEEECCCCCCHHHHHHHHHHHHEEEEECCCCCCCCCEEEEEEECCCCEEEEEECCCEEEC
SDIGRDDRAFAQFLRKAQVGEDIILKSEGQQIRSYCHVMDCASALFYILLRGKDGEAYNI
CCCCCCHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHEEEECCCCCEEEC
ANRESILSIKELAEMIANMAGVKIVYDIPSEQEVKGYSKVQRAVLDSSKLESLGWKPFIF
CCHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
LEDGLNRVLNSMMCHK
EHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]