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The map label for this gene is mleN [H]

Identifier: 150009314

GI number: 150009314

Start: 3224300

End: 3225754

Strand: Direct

Name: mleN [H]

Synonym: BDI_2716

Alternate gene names: 150009314

Gene position: 3224300-3225754 (Clockwise)

Preceding gene: 150009312

Following gene: 150009315

Centisome position: 67.01

GC content: 51.62

Gene sequence:

>1455_bases
GTGAATAAACCCATGGCAGAAAAGCCCCGGACACCCTCCATCGCGATCTCTTTACTACCCGTAGTGACATTAGTCATCAT
GCTGGCCGTAGCCATCTATATTTTCCGGGGCGATTCCCTTGGAGGAGCCAGCCAAATCGTATTATTGATAGCCACCTCGA
TCTGCTCTTTGATCGCCGTCTTTCATTGCAAGATCAAGTGGGAAGATATCGAGGGGCAAATCGCCCGTAACATCTATGGG
ATCGCACCGTCTGTCATCATCCTTTTATTGATCGGAACCCTATCGGGCAGTTGGATGGTAAGCGGGATCGTGCCGTCCTT
GATCTATTACGGGTTGCAAGTCATGCAAACGGATTTCTTCCTCGTGGCCTGTTGCCTGCTATGCAGTATCGTATCGGTCA
TGACGGGTAGCTCGTGGACTACCATCGCCACCATCGGGATCGCCTTGATCGGGATTGGCGAGGCGCAAGGCTTCAGTACG
GGGTGGGTAGCGGGAGCGATTATATCGGGCGCTTATTTCGGGGATAAGATGTCGCCGTTATCAGACACGACGGTATTGGC
GGCCTCCGTAACGGACACGCCCCTTTTCACGCATATCCGGTATATGCTTTATACGACCGTTCCCTCTATGATCGTCACAC
TGATCGTATTCAGCATCGCCGGATTCTCGAGGGAGACAGCGGACGCATCCCAGATAGCCGCTTTCTCCGAAGCCTTGAAA
GGCTCGTTCCATATCACGCCTTGGCTTATGATCGTACCGATCGTTACCGGAATCATGATCGCCAAGAAGACACCTTCGAT
CGTAGTGCTTTTCGCCTCCTCCATACTCGCCGGAATATTCGCCTTGATCTTCCAGCCAAACGCCTTATTGGAGATTTCCG
GGATCACGGATAGCGGAATTATCGCCTATATCAAAGGCCTGCTGATGACTTTCTATGACAGCACGCAGATACAGACCGGC
AACGAGGCCTTGAACTCTCTAGTCTCTACCCGGGGAATGGCCGGGATGATGAACACGATTTGGCTGATCATTTGCGCCAT
GTGTTTCGGCGGGGCCATGTCCGCTAGCGGCATGCTGGAAAGCATCACACGTATCTTCTTGCATTTCATGCGAGGCCGTA
CCAGCATGGTCGCCTCTACCGTCGTGTCCGGATTATCCTTGAACATCTGCACGGCGGACCAGTTTATCGCCATCATCCTG
AACAGCGAAATGTTTAAAGAAGTATATAAGCAACGTGGATTCGAGAGCCGCCTGCTTAGCCGGACAACGGAAGACTCAGT
CACCGTCACCTCCGTGCTGATCCCGTGGACGACTTGCGGCATGACACAATCTACAATCCTCGGCGTATCTACTTGGACTT
ATTTCCCTTATTGCGTCTTTAATATCGTCAGCCCGTTTATGAGCATTCTTATAGCGGCAACCGGTTATAAGATCGTCCAA
AAGACTGTGAAATAA

Upstream 100 bases:

>100_bases
TCCTTTTTTATTTTTGCAGCGAAATTAATTAGAATCGATAACACAAGGCAAGTCCGTATAAAGAATTTATTCGGATTATC
CATGATAAATAACAAACGAC

Downstream 100 bases:

>100_bases
GGAAATCAATCTATAATTCATAGCGTTATTCAAGGTATTTTTAGTATTTTCGCACCATCATTATTTAAGCGACTGAATGA
ACAATGGAAAATCATCTTAA

Product: Na+/H+ antiporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MNKPMAEKPRTPSIAISLLPVVTLVIMLAVAIYIFRGDSLGGASQIVLLIATSICSLIAVFHCKIKWEDIEGQIARNIYG
IAPSVIILLLIGTLSGSWMVSGIVPSLIYYGLQVMQTDFFLVACCLLCSIVSVMTGSSWTTIATIGIALIGIGEAQGFST
GWVAGAIISGAYFGDKMSPLSDTTVLAASVTDTPLFTHIRYMLYTTVPSMIVTLIVFSIAGFSRETADASQIAAFSEALK
GSFHITPWLMIVPIVTGIMIAKKTPSIVVLFASSILAGIFALIFQPNALLEISGITDSGIIAYIKGLLMTFYDSTQIQTG
NEALNSLVSTRGMAGMMNTIWLIICAMCFGGAMSASGMLESITRIFLHFMRGRTSMVASTVVSGLSLNICTADQFIAIIL
NSEMFKEVYKQRGFESRLLSRTTEDSVTVTSVLIPWTTCGMTQSTILGVSTWTYFPYCVFNIVSPFMSILIAATGYKIVQ
KTVK

Sequences:

>Translated_484_residues
MNKPMAEKPRTPSIAISLLPVVTLVIMLAVAIYIFRGDSLGGASQIVLLIATSICSLIAVFHCKIKWEDIEGQIARNIYG
IAPSVIILLLIGTLSGSWMVSGIVPSLIYYGLQVMQTDFFLVACCLLCSIVSVMTGSSWTTIATIGIALIGIGEAQGFST
GWVAGAIISGAYFGDKMSPLSDTTVLAASVTDTPLFTHIRYMLYTTVPSMIVTLIVFSIAGFSRETADASQIAAFSEALK
GSFHITPWLMIVPIVTGIMIAKKTPSIVVLFASSILAGIFALIFQPNALLEISGITDSGIIAYIKGLLMTFYDSTQIQTG
NEALNSLVSTRGMAGMMNTIWLIICAMCFGGAMSASGMLESITRIFLHFMRGRTSMVASTVVSGLSLNICTADQFIAIIL
NSEMFKEVYKQRGFESRLLSRTTEDSVTVTSVLIPWTTCGMTQSTILGVSTWTYFPYCVFNIVSPFMSILIAATGYKIVQ
KTVK
>Mature_484_residues
MNKPMAEKPRTPSIAISLLPVVTLVIMLAVAIYIFRGDSLGGASQIVLLIATSICSLIAVFHCKIKWEDIEGQIARNIYG
IAPSVIILLLIGTLSGSWMVSGIVPSLIYYGLQVMQTDFFLVACCLLCSIVSVMTGSSWTTIATIGIALIGIGEAQGFST
GWVAGAIISGAYFGDKMSPLSDTTVLAASVTDTPLFTHIRYMLYTTVPSMIVTLIVFSIAGFSRETADASQIAAFSEALK
GSFHITPWLMIVPIVTGIMIAKKTPSIVVLFASSILAGIFALIFQPNALLEISGITDSGIIAYIKGLLMTFYDSTQIQTG
NEALNSLVSTRGMAGMMNTIWLIICAMCFGGAMSASGMLESITRIFLHFMRGRTSMVASTVVSGLSLNICTADQFIAIIL
NSEMFKEVYKQRGFESRLLSRTTEDSVTVTSVLIPWTTCGMTQSTILGVSTWTYFPYCVFNIVSPFMSILIAATGYKIVQ
KTVK

Specific function: Couples proton uptake and Na(+) efflux to the substrate- product malate/lactate antiport, in an electroneutral malate- 2H(+)/Na(+)-lactate exchange. Plays a role in supporting growth to high density on malate at reduced protonmotive force [H]

COG id: COG1757

COG function: function code C; Na+/H+ antiporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nhaC Na(+)/H(+) antiporter family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004770
- InterPro:   IPR018461 [H]

Pfam domain/function: PF03553 Na_H_antiporter [H]

EC number: NA

Molecular weight: Translated: 52052; Mature: 52052

Theoretical pI: Translated: 7.87; Mature: 7.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKPMAEKPRTPSIAISLLPVVTLVIMLAVAIYIFRGDSLGGASQIVLLIATSICSLIAV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
FHCKIKWEDIEGQIARNIYGIAPSVIILLLIGTLSGSWMVSGIVPSLIYYGLQVMQTDFF
HHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
LVACCLLCSIVSVMTGSSWTTIATIGIALIGIGEAQGFSTGWVAGAIISGAYFGDKMSPL
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHCCCCCCCC
SDTTVLAASVTDTPLFTHIRYMLYTTVPSMIVTLIVFSIAGFSRETADASQIAAFSEALK
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHC
GSFHITPWLMIVPIVTGIMIAKKTPSIVVLFASSILAGIFALIFQPNALLEISGITDSGI
CCCCCCHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHCCCCEEEEECCCCCHH
IAYIKGLLMTFYDSTQIQTGNEALNSLVSTRGMAGMMNTIWLIICAMCFGGAMSASGMLE
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHH
SITRIFLHFMRGRTSMVASTVVSGLSLNICTADQFIAIILNSEMFKEVYKQRGFESRLLS
HHHHHHHHHHHCCHHHHHHHHHCCCEEEEEEHHHHHHHHHCHHHHHHHHHHCCHHHHHHH
RTTEDSVTVTSVLIPWTTCGMTQSTILGVSTWTYFPYCVFNIVSPFMSILIAATGYKIVQ
CCCCCCEEEEEEEECHHHCCCCHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
KTVK
HHCC
>Mature Secondary Structure
MNKPMAEKPRTPSIAISLLPVVTLVIMLAVAIYIFRGDSLGGASQIVLLIATSICSLIAV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
FHCKIKWEDIEGQIARNIYGIAPSVIILLLIGTLSGSWMVSGIVPSLIYYGLQVMQTDFF
HHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
LVACCLLCSIVSVMTGSSWTTIATIGIALIGIGEAQGFSTGWVAGAIISGAYFGDKMSPL
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHCCCCCCCC
SDTTVLAASVTDTPLFTHIRYMLYTTVPSMIVTLIVFSIAGFSRETADASQIAAFSEALK
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHC
GSFHITPWLMIVPIVTGIMIAKKTPSIVVLFASSILAGIFALIFQPNALLEISGITDSGI
CCCCCCHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHCCCCEEEEECCCCCHH
IAYIKGLLMTFYDSTQIQTGNEALNSLVSTRGMAGMMNTIWLIICAMCFGGAMSASGMLE
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHH
SITRIFLHFMRGRTSMVASTVVSGLSLNICTADQFIAIILNSEMFKEVYKQRGFESRLLS
HHHHHHHHHHHCCHHHHHHHHHCCCEEEEEEHHHHHHHHHCHHHHHHHHHHCCHHHHHHH
RTTEDSVTVTSVLIPWTTCGMTQSTILGVSTWTYFPYCVFNIVSPFMSILIAATGYKIVQ
CCCCCCEEEEEEEECHHHCCCCHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
KTVK
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 10903309 [H]