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The map label for this gene is mleN [H]

Identifier: 150009315

GI number: 150009315

Start: 3225838

End: 3227304

Strand: Direct

Name: mleN [H]

Synonym: BDI_2717

Alternate gene names: 150009315

Gene position: 3225838-3227304 (Clockwise)

Preceding gene: 150009314

Following gene: 150009316

Centisome position: 67.05

GC content: 49.28

Gene sequence:

>1467_bases
ATGGAAAATCATCTTAAGAAGATGCCCTCCCCTCTACTTTCACTAGTGCCTATCCTCGTGCTGGTAGCTCTTTTGTTTGT
AACCATACGCACTTTTGGCAGCGACGCGTTGAGCGGCGGAAGCCAAGTCGTATTACTTACCACCACGGCGGTGGCCTCCC
TTTTGGCCATGATCTTCTGCAAAGTCAAATGGCGTGATATAGAAGAGGCGATATGTAAAAACATATTGGGAGTGGCCACA
GCTATAATTATCCTATTGTTGATTGGTGCTTTATCCGGTAGCTGGATGATCAGCGGTATCGTCCCGACCTTGATTTATTA
CGGGATGCAAATCATCCATCCGAGTTTCTTCCTTGCCTCTTGTTGCGTGATCTGCGCTGTCGTATCCGTTATGACCGGTA
GCTCGTGGACTACCATCGCCACGATCGGTATCGCCTTGATGGGTATCGGCGAGGCACAGGGCTTCGCTACGGGATGGGTA
GCCGGAGCGATCATCTCGGGCGCTTATTTCGGGGATAAGATCTCACCGCTCTCGGACACGACGGTATTAGCTTCTTCCGT
AACGCATACCCCTTTGTTCTCCCATATCCGGTATATGATGCTTACGACAATACCGTCTATGGTGATCACGTTGATTATCT
TTACCATAGCGGGTTTTACGCATACCTCGAACGCATCCGGGCAAATAGCCGAGTTCGCCGCCTCGTTGAACGAAAGTTTT
AATATCTCGCTCTGGCTACTGATCGTACCGGTGGTAACGGGTATATTAATCGCCAAAAAAGTACCGTCTTTGATCACCTT
GTTTATCTCGACGGCGATGGCCGGGGTGTTCGCGATCTTTTTCCAGCCTCATTTATTGCAAGAGGTAGCGGGAGCGTCGG
TAGCGGATGCCTCCTCCCTGTTTAAGGGATTATTCATGACATTTTACGGGAGTACCCAGATCGATACGGGTAACGAGGCA
TTGAACAGCTTGGTCGCTACCCGGGGTATGGCGGGCATGATGAATACGATTTGGCTGATCATCTGCGCCATGTGCTTCGG
CGGCTCCATGACGGCCAGCGGTATGCTGGAAAGCCTTACGTCTGTATTCCTTCGCTTTATGAAACGGAGGGTCGGCATGG
TGGCGTCTACCGTATGTTCCGGCTTATTCTTGAATATCGTTACGGCCGACCAATACATATCCATTATCCTTACCGGAAAT
ATGTTCAAGGATATCTACAAGAAAAAAGGATATGAGAGTCGCCTGTTAAGCCGTACGACGGAAGATTCCGTAACCGTAAC
GTCCGTGCTGGTACCGTGGAACACCTGCGGCATGACGCAAGCCACGATATTAGGCGTAGCGACCCTGACGTATCTTCCTT
ATTGCTTCTTTAACCTGATCAGTCCGTTGATGAGTATTACGATGGCCGCCATTGGCTTCAAAATAAAGCAACACCATGAA
GAAGATCAGAGTATCATTGTTAACTAA

Upstream 100 bases:

>100_bases
AAAAGACTGTGAAATAAGGAAATCAATCTATAATTCATAGCGTTATTCAAGGTATTTTTAGTATTTTCGCACCATCATTA
TTTAAGCGACTGAATGAACA

Downstream 100 bases:

>100_bases
AGTCGTTATCGCTATCGCCGCCGGCGTATTGTTCGGCCAGTTTTTACCGGGAGGAATCGCACGTATCTTTGTAACCTTCA
ATTCATTGTTCGGCAACTTC

Product: Na+/H+ antiporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MENHLKKMPSPLLSLVPILVLVALLFVTIRTFGSDALSGGSQVVLLTTTAVASLLAMIFCKVKWRDIEEAICKNILGVAT
AIIILLLIGALSGSWMISGIVPTLIYYGMQIIHPSFFLASCCVICAVVSVMTGSSWTTIATIGIALMGIGEAQGFATGWV
AGAIISGAYFGDKISPLSDTTVLASSVTHTPLFSHIRYMMLTTIPSMVITLIIFTIAGFTHTSNASGQIAEFAASLNESF
NISLWLLIVPVVTGILIAKKVPSLITLFISTAMAGVFAIFFQPHLLQEVAGASVADASSLFKGLFMTFYGSTQIDTGNEA
LNSLVATRGMAGMMNTIWLIICAMCFGGSMTASGMLESLTSVFLRFMKRRVGMVASTVCSGLFLNIVTADQYISIILTGN
MFKDIYKKKGYESRLLSRTTEDSVTVTSVLVPWNTCGMTQATILGVATLTYLPYCFFNLISPLMSITMAAIGFKIKQHHE
EDQSIIVN

Sequences:

>Translated_488_residues
MENHLKKMPSPLLSLVPILVLVALLFVTIRTFGSDALSGGSQVVLLTTTAVASLLAMIFCKVKWRDIEEAICKNILGVAT
AIIILLLIGALSGSWMISGIVPTLIYYGMQIIHPSFFLASCCVICAVVSVMTGSSWTTIATIGIALMGIGEAQGFATGWV
AGAIISGAYFGDKISPLSDTTVLASSVTHTPLFSHIRYMMLTTIPSMVITLIIFTIAGFTHTSNASGQIAEFAASLNESF
NISLWLLIVPVVTGILIAKKVPSLITLFISTAMAGVFAIFFQPHLLQEVAGASVADASSLFKGLFMTFYGSTQIDTGNEA
LNSLVATRGMAGMMNTIWLIICAMCFGGSMTASGMLESLTSVFLRFMKRRVGMVASTVCSGLFLNIVTADQYISIILTGN
MFKDIYKKKGYESRLLSRTTEDSVTVTSVLVPWNTCGMTQATILGVATLTYLPYCFFNLISPLMSITMAAIGFKIKQHHE
EDQSIIVN
>Mature_488_residues
MENHLKKMPSPLLSLVPILVLVALLFVTIRTFGSDALSGGSQVVLLTTTAVASLLAMIFCKVKWRDIEEAICKNILGVAT
AIIILLLIGALSGSWMISGIVPTLIYYGMQIIHPSFFLASCCVICAVVSVMTGSSWTTIATIGIALMGIGEAQGFATGWV
AGAIISGAYFGDKISPLSDTTVLASSVTHTPLFSHIRYMMLTTIPSMVITLIIFTIAGFTHTSNASGQIAEFAASLNESF
NISLWLLIVPVVTGILIAKKVPSLITLFISTAMAGVFAIFFQPHLLQEVAGASVADASSLFKGLFMTFYGSTQIDTGNEA
LNSLVATRGMAGMMNTIWLIICAMCFGGSMTASGMLESLTSVFLRFMKRRVGMVASTVCSGLFLNIVTADQYISIILTGN
MFKDIYKKKGYESRLLSRTTEDSVTVTSVLVPWNTCGMTQATILGVATLTYLPYCFFNLISPLMSITMAAIGFKIKQHHE
EDQSIIVN

Specific function: Couples proton uptake and Na(+) efflux to the substrate- product malate/lactate antiport, in an electroneutral malate- 2H(+)/Na(+)-lactate exchange. Plays a role in supporting growth to high density on malate at reduced protonmotive force [H]

COG id: COG1757

COG function: function code C; Na+/H+ antiporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nhaC Na(+)/H(+) antiporter family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004770
- InterPro:   IPR018461 [H]

Pfam domain/function: PF03553 Na_H_antiporter [H]

EC number: NA

Molecular weight: Translated: 52329; Mature: 52329

Theoretical pI: Translated: 7.91; Mature: 7.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENHLKKMPSPLLSLVPILVLVALLFVTIRTFGSDALSGGSQVVLLTTTAVASLLAMIFC
CCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCEEEEEEHHHHHHHHHHHHH
KVKWRDIEEAICKNILGVATAIIILLLIGALSGSWMISGIVPTLIYYGMQIIHPSFFLAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
CCVICAVVSVMTGSSWTTIATIGIALMGIGEAQGFATGWVAGAIISGAYFGDKISPLSDT
HHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCH
TVLASSVTHTPLFSHIRYMMLTTIPSMVITLIIFTIAGFTHTSNASGQIAEFAASLNESF
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCC
NISLWLLIVPVVTGILIAKKVPSLITLFISTAMAGVFAIFFQPHLLQEVAGASVADASSL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
FKGLFMTFYGSTQIDTGNEALNSLVATRGMAGMMNTIWLIICAMCFGGSMTASGMLESLT
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
SVFLRFMKRRVGMVASTVCSGLFLNIVTADQYISIILTGNMFKDIYKKKGYESRLLSRTT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECHHHHHHHHHCCHHHHHHHCCC
EDSVTVTSVLVPWNTCGMTQATILGVATLTYLPYCFFNLISPLMSITMAAIGFKIKQHHE
CCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EDQSIIVN
CCCCEECC
>Mature Secondary Structure
MENHLKKMPSPLLSLVPILVLVALLFVTIRTFGSDALSGGSQVVLLTTTAVASLLAMIFC
CCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCEEEEEEHHHHHHHHHHHHH
KVKWRDIEEAICKNILGVATAIIILLLIGALSGSWMISGIVPTLIYYGMQIIHPSFFLAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
CCVICAVVSVMTGSSWTTIATIGIALMGIGEAQGFATGWVAGAIISGAYFGDKISPLSDT
HHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCH
TVLASSVTHTPLFSHIRYMMLTTIPSMVITLIIFTIAGFTHTSNASGQIAEFAASLNESF
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCC
NISLWLLIVPVVTGILIAKKVPSLITLFISTAMAGVFAIFFQPHLLQEVAGASVADASSL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
FKGLFMTFYGSTQIDTGNEALNSLVATRGMAGMMNTIWLIICAMCFGGSMTASGMLESLT
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
SVFLRFMKRRVGMVASTVCSGLFLNIVTADQYISIILTGNMFKDIYKKKGYESRLLSRTT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECHHHHHHHHHCCHHHHHHHCCC
EDSVTVTSVLVPWNTCGMTQATILGVATLTYLPYCFFNLISPLMSITMAAIGFKIKQHHE
CCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EDQSIIVN
CCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 10903309 [H]