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The map label for this gene is murE

Identifier: 150009090

GI number: 150009090

Start: 2936500

End: 2937960

Strand: Reverse

Name: murE

Synonym: BDI_2491

Alternate gene names: 150009090

Gene position: 2937960-2936500 (Counterclockwise)

Preceding gene: 150009091

Following gene: 150009089

Centisome position: 61.06

GC content: 50.86

Gene sequence:

>1461_bases
ATGGAACTAAGGGATTTAATCGGTGCGTTGGAAGCTCCTATGTTGATGGGGGCGGATAATGTGGATATCACGAATATTCA
ATCAGACTCTCGTCGGGTGACGAAAGGGTCTTTGTTTGTCGCTGTGAGAGGTACGGCCGTGGATGGCCATGCCTATATGG
ACAGCGCCATAGAGAAAGGGGCTGTCGCTATCGTATGCGAGGAGACTCCGTCCTATTTGGAGGGGAGATGCTCGTTTATC
GTGGTGAAGGATTCGGCGGAGGCACTAGGTCTTTTGGTTTCCCGCTGGTATGGCGATCCTTCCCGGAAATTGATGCTGGT
GGGCGTGACGGGTACGAACGGGAAGACTACGATCGCTACCTTATTATATGAGATGTTCCGGAAGATGGGGCATAAGGTGG
GATTGCTTTCCACGGTATGTAATTATATAGATGGCGAGGCGGTACCGACGGATCATACGACACCGGACCCGCTTACGCTT
CATTCCTTGATGGCCCGGATGGTAGAGGCCGGATGCGAGTATGCTTTTATGGAGGTTAGCTCTCATTCGATCGATCAGCG
GCGCATCAGCGGCTTGTCTTTCGACGGGGGGATTTTCACGAACCTGACCCGCGATCATTTGGATTACCATAAGACCGTGG
AGAATTACTTGAAAGCGAAGAAGAAATTCTTCGATGACCTGCCTGCCGGGACTTTTGCCTTGACGAATGCCGATGATAAA
TCCGGCTTGGTGATGTTGCAGAATACGAAGGCGAAGAAGCTGACCTATTCCTTGCGTACCTTGGCCGATTTCAAGGGAAA
GATATTGGAATCTCATTTCGAGGGTACAGACTTGATCATTAACGGTCGGGAGGTTATGGTGCATTTCGTGGGACGTTTCA
ACGCTTATAACTTGCTGGCCGTGTATGGTGCTGCGGTTTCTTTAGGGAAAGATCCCGAGGAGGTGTTGATCGTGCTGAGC
ACGCTTCGCTCCGTATCCGGTCGCTTCGAGACGATCCAGTCTCCGCTCGGTTATACGGCGATCGTGGATTATGCTCACAC
ACCGGATGCCTTGACGAATGTACTGAATGGCATTCATGAGGTATTGGATGGGAAAGGACGTATCATTACCGTCGTGGGTG
CCGGGGGAAACCGGGACAAGGGGAAACGCCCGCTGATGGCGAAAGAGGCTGTCAAGCTGAGCGATCAGGTAATTCTGACT
TCCGATAACCCGCGCTTTGAGGAACCGGATGATATTATTAACGATATGGTAGCCGGATTGACGAAAATGGATATGGAACG
TACCTTATGTATCACGGACCGTACGCAAGCGATCAAGACCGCTACGATGTTGGCGAAGAAAGGGGATGTGATCCTTGTTG
CCGGAAAAGGCCACGAAGACTATCAGGAGATCAAGGGGGTGAAACAGCACTTTGACGACCGTGAGAAGTTACGCGAGATT
TTTTCGACACAACAATCATAA

Upstream 100 bases:

>100_bases
GTGTCTCTTTGTCGGGTGTCGGACGGGTCGTTTCGCAGAGTATTCAACCCGGACAGCGAGTGTCTAAGGGGCAGACCGTA
TTATTAACATTGAAATAGGT

Downstream 100 bases:

>100_bases
TATTTAAGACGACATGCTTTATTATCTTTTTAATTATTTGGATCAGTTGGATTTCCCGGGAGCGGGTATGTTTAAGTACG
TATCTTTCCGCTCGGGGTTG

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MELRDLIGALEAPMLMGADNVDITNIQSDSRRVTKGSLFVAVRGTAVDGHAYMDSAIEKGAVAIVCEETPSYLEGRCSFI
VVKDSAEALGLLVSRWYGDPSRKLMLVGVTGTNGKTTIATLLYEMFRKMGHKVGLLSTVCNYIDGEAVPTDHTTPDPLTL
HSLMARMVEAGCEYAFMEVSSHSIDQRRISGLSFDGGIFTNLTRDHLDYHKTVENYLKAKKKFFDDLPAGTFALTNADDK
SGLVMLQNTKAKKLTYSLRTLADFKGKILESHFEGTDLIINGREVMVHFVGRFNAYNLLAVYGAAVSLGKDPEEVLIVLS
TLRSVSGRFETIQSPLGYTAIVDYAHTPDALTNVLNGIHEVLDGKGRIITVVGAGGNRDKGKRPLMAKEAVKLSDQVILT
SDNPRFEEPDDIINDMVAGLTKMDMERTLCITDRTQAIKTATMLAKKGDVILVAGKGHEDYQEIKGVKQHFDDREKLREI
FSTQQS

Sequences:

>Translated_486_residues
MELRDLIGALEAPMLMGADNVDITNIQSDSRRVTKGSLFVAVRGTAVDGHAYMDSAIEKGAVAIVCEETPSYLEGRCSFI
VVKDSAEALGLLVSRWYGDPSRKLMLVGVTGTNGKTTIATLLYEMFRKMGHKVGLLSTVCNYIDGEAVPTDHTTPDPLTL
HSLMARMVEAGCEYAFMEVSSHSIDQRRISGLSFDGGIFTNLTRDHLDYHKTVENYLKAKKKFFDDLPAGTFALTNADDK
SGLVMLQNTKAKKLTYSLRTLADFKGKILESHFEGTDLIINGREVMVHFVGRFNAYNLLAVYGAAVSLGKDPEEVLIVLS
TLRSVSGRFETIQSPLGYTAIVDYAHTPDALTNVLNGIHEVLDGKGRIITVVGAGGNRDKGKRPLMAKEAVKLSDQVILT
SDNPRFEEPDDIINDMVAGLTKMDMERTLCITDRTQAIKTATMLAKKGDVILVAGKGHEDYQEIKGVKQHFDDREKLREI
FSTQQS
>Mature_486_residues
MELRDLIGALEAPMLMGADNVDITNIQSDSRRVTKGSLFVAVRGTAVDGHAYMDSAIEKGAVAIVCEETPSYLEGRCSFI
VVKDSAEALGLLVSRWYGDPSRKLMLVGVTGTNGKTTIATLLYEMFRKMGHKVGLLSTVCNYIDGEAVPTDHTTPDPLTL
HSLMARMVEAGCEYAFMEVSSHSIDQRRISGLSFDGGIFTNLTRDHLDYHKTVENYLKAKKKFFDDLPAGTFALTNADDK
SGLVMLQNTKAKKLTYSLRTLADFKGKILESHFEGTDLIINGREVMVHFVGRFNAYNLLAVYGAAVSLGKDPEEVLIVLS
TLRSVSGRFETIQSPLGYTAIVDYAHTPDALTNVLNGIHEVLDGKGRIITVVGAGGNRDKGKRPLMAKEAVKLSDQVILT
SDNPRFEEPDDIINDMVAGLTKMDMERTLCITDRTQAIKTATMLAKKGDVILVAGKGHEDYQEIKGVKQHFDDREKLREI
FSTQQS

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily

Homologues:

Organism=Escherichia coli, GI1786273, Length=488, Percent_Identity=37.2950819672131, Blast_Score=263, Evalue=3e-71,
Organism=Escherichia coli, GI1786274, Length=329, Percent_Identity=26.1398176291793, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURE_PARD8 (A6LEU7)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001303833.1
- ProteinModelPortal:   A6LEU7
- SMR:   A6LEU7
- STRING:   A6LEU7
- GeneID:   5307640
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_2491
- eggNOG:   COG0769
- HOGENOM:   HBG602753
- OMA:   GHEEGQT
- ProtClustDB:   PRK00139
- BioCyc:   PDIS435591:BDI_2491-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00208
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- TIGRFAMs:   TIGR01085

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.13

Molecular weight: Translated: 53398; Mature: 53398

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: NA

Important sites: BINDING 30-30 BINDING 180-180 BINDING 186-186 BINDING 188-188 BINDING 378-378 BINDING 455-455 BINDING 459-459

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELRDLIGALEAPMLMGADNVDITNIQSDSRRVTKGSLFVAVRGTAVDGHAYMDSAIEKG
CCHHHHHHHHCCCCEECCCCCEEECCCCCCCEEECCEEEEEEECEEECCHHHHHHHHCCC
AVAIVCEETPSYLEGRCSFIVVKDSAEALGLLVSRWYGDPSRKLMLVGVTGTNGKTTIAT
CEEEEECCCCCCCCCCEEEEEEECCHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHH
LLYEMFRKMGHKVGLLSTVCNYIDGEAVPTDHTTPDPLTLHSLMARMVEAGCEYAFMEVS
HHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECC
SHSIDQRRISGLSFDGGIFTNLTRDHLDYHKTVENYLKAKKKFFDDLPAGTFALTNADDK
CCCCHHHHHCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCC
SGLVMLQNTKAKKLTYSLRTLADFKGKILESHFEGTDLIINGREVMVHFVGRFNAYNLLA
CCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHCCCHHHHH
VYGAAVSLGKDPEEVLIVLSTLRSVSGRFETIQSPLGYTAIVDYAHTPDALTNVLNGIHE
HHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHH
VLDGKGRIITVVGAGGNRDKGKRPLMAKEAVKLSDQVILTSDNPRFEEPDDIINDMVAGL
HHCCCCCEEEEEECCCCCCCCCCCCHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHH
TKMDMERTLCITDRTQAIKTATMLAKKGDVILVAGKGHEDYQEIKGVKQHFDDREKLREI
HHHCCCCEEEECCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCHHHHHHHH
FSTQQS
HCCCCC
>Mature Secondary Structure
MELRDLIGALEAPMLMGADNVDITNIQSDSRRVTKGSLFVAVRGTAVDGHAYMDSAIEKG
CCHHHHHHHHCCCCEECCCCCEEECCCCCCCEEECCEEEEEEECEEECCHHHHHHHHCCC
AVAIVCEETPSYLEGRCSFIVVKDSAEALGLLVSRWYGDPSRKLMLVGVTGTNGKTTIAT
CEEEEECCCCCCCCCCEEEEEEECCHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHH
LLYEMFRKMGHKVGLLSTVCNYIDGEAVPTDHTTPDPLTLHSLMARMVEAGCEYAFMEVS
HHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECC
SHSIDQRRISGLSFDGGIFTNLTRDHLDYHKTVENYLKAKKKFFDDLPAGTFALTNADDK
CCCCHHHHHCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCC
SGLVMLQNTKAKKLTYSLRTLADFKGKILESHFEGTDLIINGREVMVHFVGRFNAYNLLA
CCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHCCCHHHHH
VYGAAVSLGKDPEEVLIVLSTLRSVSGRFETIQSPLGYTAIVDYAHTPDALTNVLNGIHE
HHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHH
VLDGKGRIITVVGAGGNRDKGKRPLMAKEAVKLSDQVILTSDNPRFEEPDDIINDMVAGL
HHCCCCCEEEEEECCCCCCCCCCCCHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHH
TKMDMERTLCITDRTQAIKTATMLAKKGDVILVAGKGHEDYQEIKGVKQHFDDREKLREI
HHHCCCCEEEECCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCHHHHHHHH
FSTQQS
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA